Elenco delle attività formative previste per i dottorandi del secondo anno |
Natural organic compounds of food and pharmaceutical interest
data presunta: 12/2025 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 8
docente del corso: Dott. Marco Franceschin qualifica: Ricercatore affiliazione: Italiana
programma delle attività: Learning outcomes
The aim of the course is to connect basic knowledge of organic chemistry and pharmaceutical chemistry to the field of natural organic compounds. Indeed, natural organic compounds represent a very wide range of different compounds of food and pharmaceutical interest. During the course, the main biogenetic pathways and structural classification will be presented. At the end of the course, students should be able to recognize active substances in medicines and food that are of biological origins, suggesting the classes to which they belong. Moreover, a focus on food and drug additives as well as module 3 “quality” of the dossier and basis of GMP will be made for substances of natural origin.
Syllabus
• Techniques for the study and characterization of secondary metabolites, main biogenetic pathways
• Acetate and mevalonate pathway: terpenes and cannabinoids
• Shikimic acid pathway: flavonoids
• Examples of secondary metabolites with known biological activity
• Alkaloids: examples of natural antitumoral compounds
• Natural antioxidants (polyphenols, vitamin E and vitamin C)
• Nutrition facts label: food and drug additives (preservatives and colorants)
• module 3 “quality” of the dossier: structure and information; basis of GMP (Good Manufacturing Practice)
modalità di accertamento finale: Test di verifica finale
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Multidimensional mass spectrometry for applications in the (bio) chemical, pharmaceutical and food fields
data presunta: 01/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 8
docente del corso: Dott. Davide Corinti qualifica: Ricercatore affiliazione: Italiana
programma delle attività: Learning outcomes
Main goals of this course are:
• to provide an introduction to mass spectrometry and its hyphenated techniques (Ion Mobility, MALDI imaging, CID, UVPD, etc.);
• to demonstrate and discuss applications of MS and related techniques in biological, pharmaceutical and food chemistry;
At the end of the course, students should be able to:
• understand the basic concepts of mass spectrometry and related techniques;
• demonstrate an understanding of how and why mass spectrometry can be applied to life sciences;
• critically assess current literature on mass spectrometry analyses.
In addition to theoretical lectures, students will attend practical lessons on mass spectrometry experiments.
Syllabus
• Introduction to mass spectrometry.
• Ion sources (ESI, APCI, MALDI, APPI) and their application in life sciences.
• Ion activation methods (CID, UVPD, IRPD, EXD) with examples.
• Ion mobility-MS and its use for complex biological matrices and native mass spectrometry of biomolecular ions.
• MALDI imaging for clinical diagnosis and analysis of biological tissues.
modalità di accertamento finale: Test di verifica finale
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Development of NMR protocols for food analysis
data presunta: 02/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 8
docente del corso: Dott. Mattia Spano qualifica: Ricercatore affiliazione: Italiana
programma delle attività: Learning outcomes
The objective of the course is to train the students in developing a NMR protocol for the study of foods, from sampling to data processing. The aspects involved in the analytical protocol development will be considered: sampling, differences between liquid and solid samples, extraction methods, sample preparation for NMR analysis, NMR parameters for experiment setup, data processing.
An example of NMR metabolomics application will be discussed in the context of innovative food matrices namely Novel Foods.
In this context, Novel Food field will be discussed in terms of in terms of legislative regulation, scientific process for new matrices approval, food safety, nutritional benefits and potential risks.
Syllabus
• Sampling approaches.
• Extraction methods for NMR analysis.
• NMR sample preparation (solvent, buffer, internal standard, stabilizers)
• Acquisition parameters for NMR experiments
• NMR spectra processing
• Novel Foods: legislation, new matrices characterization, nutritional benefits and risks, novel food approval
modalità di accertamento finale: Test di verifica finale
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Advanced methodologies for studying complex matrices
data presunta: 03/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 8
docente del corso: Prof Andrea Calcaterra qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes:
Rational retrosynthetic analysis of selected compounds through the disconnection of key bonds. Identification of synthons and suitable starting materials. Rational design of synthetic strategy of target compounds based on the retrosynthetic approach. Application of key reactions to solve synthetic problems.
Syllabus:
Classifications in synthetic methodology. Retrosynthetic analysis. Synthons and reagents. Group disconnections. Regio- and chemoselectivity. Useful reactions in total synthesis. Control of stereochemistry. Catalytic asymmetric reactions. Biosynthesis of selected alkaloids. Synthetic procedures for the construction of various alkaloid scaffolds: tetrahydroisoquinoline alkaloids, monoterpene indole alkaloids (iboga and aspidosperma alkaloids), indole diterpene alkaloids.
Selected total synthesis: thyroxine, longifolene, reserpine, strychnine, vinblastine and vincristine, estrone, menthol, taxol, epothilone A. Overview on the synthesis of complex marine natural products.
modalità di accertamento finale: Test di verifica finale
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Chiral photon/chiral molecule interaction as a spectroscopic tool for chiral recognition
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Stefano Stranges qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
Contents provided in the lectures will allow acquiring a deeper knowledge of basic concepts of molecular chirality and optical activity, fundamental aspects in both food and drug sciences, as well as of different approaches to characterize optical active molecules. Special focus is given on the recently developed PhotoElectron Circular Dichroism (PECD) technique in the context of chiral recognition, when this approach involves synchrotron radiation and state-of-the-art theoretical models.
Syllabus
• Optical activity and chirality. Optical activity and Group Theory. Molecular symmetry: dissymmetric and asymmetric molecules.
• Optical activity manifestations as radiation absorption, dispersion, and emission (CD, ORD, CPL). Radiation polarizations, basic concepts and light-matter interaction. Optical Rotation Dispersion and Circular Dichroism of chiral samples.
• Synchrotron radiation accelerators and Elettra Laboratory as ideal polarized photon source. Key features of SR. Photoabsorption and photoemission Circular Dichroism, NCD and PECD.
• PECD experiment and theory synergism as powerful tool for chiral recognition.
• Perspectives in PECD research activity of molecular chirality. Recent results on model chiral molecules and foreseen developments
modalità di accertamento finale:
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Exploring the intrinsic properties of bioinorganic complexes by ion-molecule reactions
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Elisa Crestoni qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
The objective of the course is to provide fundamental knowledge regarding ion-molecule reactions (IMRs) that can be carried out in the gas phase inside an ion trap mass spectrometer, like FT-ICR MS. An important advantage to working in the gas phase, an environment lacking solvent and counter ions, is that the intrinsic behavior of ionic species can be elucidated. Some general aspects including how to measure the rate constants and obtain the yield of the reaction, will be illustrated, and discussed in relation to the double well potential energy profile scheme, besides a description of the best suited equipment for IRMs. Several types of IMRs have been developed and applications in analytical chemistry, biochemistry and astrochemistry will be offered taken from recent example of literature.
Syllabus
• FT-ICR MS methodology to perform IMRs;
• How to measure the experimental rate constants; how to calculate the collision rate constant and the efficiency of the reaction;
• Types of IMRs: electron-transfer; proton-transfer; H/D exchange; H/D echange; nucleophilic displacement; radiative association;
• Applications: determination of thermodynamic and kinetic parameters; chiral recognition; functional-group selective identification; oxygen atom transfer from high-valent metal-oxo complexes of bioinorganic relevance; effect of local environment in phosphorylated peptides; coordination of prosthetic group in systems of increased complexity.
modalità di accertamento finale:
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A dynamic approach to investigate the stereochemical (in)stability of chiral compounds
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Alessia Ciogli qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
At the end of course, the PhD student will be familiar with the dynamic techniques helpful to investigate flexible molecules and he could approach the development of enantioselective chromatographic methods.
Syllabus
The presence of stereogenic elements is a common feature in pharmaceutical and biopharmaceutical compounds and demand of optically pure stereoisomers is a frequent issue in drug design:
• The stereochemical lability of chiral species.
• Theoretical aspect racemization and diasteromerization processes
• Off-column approach: classical kinetic techniques.
• On-column approach: dynamic HPLC and dynamic NMR.
• An overview of both off-column and on-column approaches will be presented followed by significant examples of flexible (conformational and configurational) molecules of pharmaceutical interest.
modalità di accertamento finale:
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NMR methodologies in the study of complex matrices
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Luisa Mannina qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
The aim of this course is to provide knowledge regarding the application of NMR based metabolomic in the study of biological fluids to reveal specific diseases and the effect of particular dietetic regimes. Crucial aspects of the NMR methodology as well of the chemometric approach will be discussed. Different applications will be reported. The student will know the NMR spectra of some biological fluids such urines and saliva and the different ways to carried out the metabolite Moreover, the student will understand and critically evaluate the best chemometric approach to be used according to the specific problem
Syllabus
NMR methodologies.
Samples preparation for NMR analysis.
NMR spectra of biological fluids and metabolite quantification.
Chemometric analysis.
modalità di accertamento finale:
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Advanced ion spectroscopy techniques: identification and structural characterization of bioactive molecules
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Barbara Chiavarino qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
Topics of the module include:
• Gas Phase IR Spectroscopy of Ions and IRMPD Specificities: introduction of the technique.
• Description the experimental set-ups:
1. Ion trap mass spectrometers (Paul trap and FT-ICR mass spectrometers) coupled with different ion sources (IE, CI and ESI)
2. Powerful infrared tunable laser sources: IR free electron lasers (IR-FEL’s) and tabletop optical parametric oscillator/amplifier OPO/OPA lasers.
• Application to the IRMPD to elucidate the structure of gaseous molecular ions or ion-molecule complexes.
• Kinetic measurements
Syllabus
• Overview of a relatively novel approach to perform vibrational spectroscopy of gaseous ions using the tools of mass spectrometry.
• Perspectives of the application of IR multi-photon dissociation (IRMPD) action spectroscopy to obtain structural information on ions of fundamental, biological, pharmaceutical interest and its recent application in mass spectrometry-based metabolomics analysis.
• Understand the principles and application fields of IRMPD spectroscopy.
• Design and identify which systems can be successfully studied with ion spectroscopy.
• Critically compare experimental with theoretical IR spectra.
• Analytically estimate the current literature on IRMPD action spectroscopy.
modalità di accertamento finale:
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Click chemistry, a powerful molecular assembly tool for the widest range of applications.
data presunta: 04/2026 - tipologia: riconducibile al progetto formativo - modalità di erogazione: Ex-cathedra - numero ore: 2
docente del corso: Prof Giancarlo Fabrizi qualifica: Professore affiliazione: Italiana
programma delle attività: Learning outcomes
At the end of course, the PhD student will be familiar with the following topics:
• Click chemistry principles
• Click chemistry reaction types
• Clip chemistry
• Mainly bimolecular applications of click chemistry.
Syllabus
• Metal-catalyzed click reactions
• Metal-free click reactions
• Click reactions promoted by light and oxidation
• 4. Clip chemistry: rapid and regioselective disconnection.
modalità di accertamento finale:
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