Matrix Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI), a multicomponent imaging technique able to detect and localize several compounds onto a tissue
sample in the same run. Because it can deliver in-situ spatial visualization of biologically relevant compounds (e.g. peptides, lipids, metabolites and xenobiotics) this tissue profiling
based on mass spectrometry has found several applications from histopathology to drug development.
The association between lipid metabolism and long-term outcomes is relevant for tumor diagnosis and therapy. Archival material such as formalin-fixed and paraffin embedded (FFPE) tissues is a highly valuable resource for this aim as it is linked to long-term clinical follow-up.
Therefore, there is a need to develop robust methodologies able to detect lipids in FFPE material and correlate them with clinical outcomes. This seminar will talk about the use of MALDI-MSI to reveal and spatially resolve lipid alterations in tumor specimens as FFPE. This analysis together with hyphenated techniques aims also to obtain further information about cellular membrane composition in cancer as a future perspective.
20/12/2019
Dr. Marco Giampà-Department of Clinical and Molecular Medicine, NTNU–Norwegian University of Science and Technology 11:30, Aula B, CU019