The annual report must be drawn up following the specimen available in the indicated section and must be sent to the Doctorate Secretariat Mrs. Angela Tilia (
amministrazionedottorati.dsb@uniroma1.it) by 1 October.
During the FIRST YEAR OF THE COURSE, the doctoral students of the 39th cycle regularly attended the seminars (in presence, in room D of Forensic Medicine, as indicated in the "Seminars" section of the website) and the educational activities organized by the Teaching Board and the BeMM Doctoral School (see “Training offer” section). They also participated in the JOURNAL CLUB (see website section) and defined the thesis project project. Furthermore, they participated in courses and conferences. The lines of research addressed are the following:: “Stabilità strutturale e funzionale della proteina PTPN3 e delle sue varianti somatiche missenso in tessuti tumorali-Structural analysis of protein variants involved in cancer- Innovative approaches to identify and optimize new therapeutic agents for the treatment of infectious and genetic diseases- Developing a ferritin base disruptor nanoparticle: a potential treatment for hypercholesterolemia- Development of targeted transport systems for active substances such as drugs- : Cryo-EM structure of Apo-PdxR from Bacillus clausii in complex with its target DNA- Structural Bioinformatics applied to the analysis of proteins involved in pathological processes and their interaction with small molecules- Exploring the biochemical properties of the proteins MINT 2 and MAGI 1 including their folding pathways and binding interactions- Dys-regulated protein O-GlcNacylation in the hippocampus of Rett syndrome mouse models: A promising molecular link between metabolic signalling and neuronal damage-Dipeptidyl peptidase 4 activity is associated with accelerated aging in down syndrome .Tra le conferenze: 37th EPS European Peptide Symposium” Florence, from August 25 to 29, 2024.- “48th FEBS Congress, 29 June – 3 July 2024”, Milan.At the end of the year, the results of their research were summarized in a written report and presented to the collegial Progress Reports (see poster in the Insights section of the website) for admission to the following year which is approved by the teaching board based on the written report and the PROGRESS REPORT
During the SECOND YEAR OF THE COURSE, the doctoral students of the 38th cycle regularly attended the seminars (in Forensic Medicine room D, as indicated in the "Seminars" section of the website); the teaching activities organized by the Teaching Board and the BeMM Doctoral School (see "Educational offer" section) and continued the experimental activities relating to their thesis project Furthermore, they participated in conferences relevant to their lines of research, listed below:: - Expression, purification and characterization of PA2072 by Pseudomonas aeruginosa- Characterization of Down Syndrome through identification and prioritization of rare and unique genomic variants on whole genome sequencing data- Addressing the communication pathways of contiguous domains in a multi-domain system- folding and binding- STAT3 involvement in energy metabolism of cancer cells- Studies on the inhibition of pyridox(am)ine 5’-phosphate oxidase- Development of selective inhibitors for the PDIA3 protein for pharmacological use - HPV and new therapeutic strategy- Development of a predictor for the inference of protein variants associated with rare diseases whose activity can be stabilized or recovered by means of second generation pharmacological chaperones”- Structural and functional characterization of proteins from Pseudomonas aeruginosa- Molecular mechanisms responsible for metabolic alterations in neurodegenerative process - Role of miR-802 in insulin signaling and its impact on neurodegeneration”- Study of new diagnostic and prognostic markers in protein misfolding diseases- Alteration of metabolic pathways in tumors”. At the end of the year, the results of their research were summarized in a written report and presented to the collegial Progress Reports (see poster in the Insights section of the website) for admission to the following year which is approved by the teaching body on the basis of the written report and the PROGRESS REPORT
During the THIRD YEAR OF THE COURSE, doctoral students of the 37th cycle attended the lessons and seminars organized by teaching board and the BeMM Doctoral School and concluded the experimental activities relating to the thesis project. They did it to the drafting of the final thesis and all the theses were uploaded to the doctoral office website. The titles of the theses are as follows:: Investigating the Ferroportin-Ferroxidase System- Deciphering the activation mechanism of Sigma 1 Receptor and the nature of its endogenous ligands- The role of selected single-point mutations in the Fragile-X Messenger Ribonucleoprotein: from (mis)folding properties to Liquid--Liquid Phase Separation- Structural and functional characterization of a humanized neutralizing antibody targeting the SARS-CoV-2 Receptor Binding Domain (RBD)- Nitric Oxide and Hydrogen Sulfide interplay and tolerance in Pseudomonas aeruginosa: role of sulfide catabolism and aerobic respiration- Deciphering the interplay between the Unfolded Protein Response and metabolic defects in Down syndrome neuropathology- Targeting mitochodrial homeostasis with small molecules: from environmental contaminants to inhibitors of nucleotide recycling- Engineered human ferritins for the selective delivery of bioactive molecules to pathological sites. Le dottorande Megliorini e Torrieri di Tullio conseguiranno il titolo di dottorato nelle prossime sessioni al termine dei 36 mesi di attività
RESULTS OF 2022-2023
During the
FIRST YEAR OF THE COURSE, the doctoral students of the 38th cycle regularly attended the seminarsand the teaching activities organized by the Teaching Body and the BeMM Doctoral School and defined the thesis project. Furthermore, they participated in courses and conferences. The lines of research addressed are the following:
- Expression, purification and characterization of the diguanilate cyclase PA14_0847 by Pseudomonas aeruginosa- “Integration of multi-omics data for the development of a predictor to infer Trisomy 21 severity”- Comparison of the folding and the binding features of domains in isolation and in a multidomain construct”- STAT3 involvement in energy metabolism of cancer cells.- Studies on the inhibition of pyridox(am)ine 5’-phosphate oxidase- DEVELOPMENT OF SELECTIVE INHIBITORS FOR THE PDIA3 PROTEIN FOR PHARMACOLOGICAL USE- HPV and lesions: new therapeutic strategy- Development of a predictor for the inference of protein variants associated with rare diseases whose activity can be stabilized or recovered by means of second generation pharmacological chaperones”- “Development of a predictor for the inference of protein variants associated with rare diseases whose activity can be stabilized or recovered by means of second generation pharmacological chaperones”- Structural and functional characterization of proteins involved in H2S homeostasis and in lipid transport in Pseudomonas aeruginosa- “Molecular mechanisms responsible for metabolic alterations in neurodegenerative process - Role of miR-802 in insulin signaling and its impact on neurodegeneration”- Study of new diagnostic and prognostic markers in protein misfolding diseases- Alteration of metabolic pathways in tumors
During the
SECOND YEAR OF THE COURSE, the 37th cycle doctoral students regularly attended the seminars and activities organized by teaching board and the BeMM Doctoral School and continued the experimental activities relating to their thesis project. Furthermore, they participated in conferences relevant to their lines of research, listed below:- “Study of the Ferroportin-Ceruloplasmin system”- Structure-function relationships in Sigma 1 Receptor- Characterization of KH domains of FMRP, a protein involved in the Fragile X syndrome.”- Development and characterization of neutralizing antibodies against SARS-CoV2-Biochemical characterization of bacterial hydrogen sulfide metabolism: the case of
Pseudomonas aeruginosa- Development of a low-intensity pulsed ultrasound print-head to drive the differentiation of 3D bioprinted skeletal stem cells- Molecular mechanism of Alzheimer-like neuropathology in Down Syndrome (DS) associated with loss of proteostasis- Nitro-oxidative stress induced by relevant environmental contaminants in human cell lines- ENGINEERED HUMAN FERRITINS FOR CANCER THERAPY .
Among conferences:-34th meeting "Riunione Nazionale dei Dottorandi di Ricerca in Discipline Biochimiche", Brallo di Pregola (PV)-62° SIB Congress – 2023, Firenze.-ERICE 2023 International School of Crystallography 58th Course-The 47
th FEBS Congress, 2023 – Tours, France-“8
th International School on Biological Crystallization” 21/26 May 2023, Granada, Spain.- Italian Crystallographic Association: “4rd Meeting of the Biological MacroMolecules Section” 5-6 June 2023, Fiesole (FI), Italy.
During the
THIRD YEAR OF THE COURSE, the 36th cycle doctoral students attended the lessons and seminars organized by teaching board and the BeMM Doctoral School and concluded the experimental activities relating to the thesis project. They did it to the drafting of the final thesis and all the theses were uploaded to the doctoral office website. The titles of the theses are as follows:-- Use of Olive Oil Polyphenols to Counteract Neuroinflammation and Neurodegenerative Diseases- Sex-dependent Brain-specific Alterations of Insulin Signalling Uncover Early Molecular Mechanisms Leading to Cognitive Decline- Inhibiting STAT3 Signaling Pathways by Using Natural Compounds to Modulate Chemical Carcinogenesis- In Silico Design and Evaluation of Exon Skipping-Inducing Antisense Oligonucleotides for a Potential Therapeutic Intervention in Cancer- Structural Bioinformatics: Methods Development and Applications to Biomedical Problems- Insights into the Mechanism(s) of Action of the Membrane-Active HostDefence Esc Peptides on Model and Living Systems- Macromolecular Interactions in Solution – Interferometric and Turbidimetric Studies
RESULTS OF 2021-2022
During the
FIRST YEAR OF THE COURSE, the doctoral students of the 37th cycle regularly attended the seminars (in electronic mode) and the teaching activities organized by the Teaching Body and the BeMM Doctoral School and defined the thesis project. Furthermore, they participated in courses and conferences organized, mainly, remotely. The lines of research addressed are the following: - Study of ferroxidase-permease systems involved in the transport of iron across membranes -Study of the structure-activity relationship in the Sigma 1 receptor -Biochemical characterization of the KH domains of FMRP, a protein involved in fragile X syndrome -Development and characterization of neutralizing antibodies for the treatment of COVID-19 -Biochemical characterization of bacterial metabolism of hydrogen sulphide: the case of Pseudomonas aeruginosa -3D bone bioprinting through direct differentiation of stem cells using Low Intensity Pulsed Ultrasound: study of the biological effects and pathways activated in vitro and in vivo by LIPUS. -Development of a "multi-omics" data integration platform for the inference of molecular interaction networks between pathogen and target cell -Theranostic nanosystems for the Drug Targeting of Sunitinib Maleate -Modulation of nitro-oxidative stress in eukaryotic cells: involvement of the nitric oxide signaling pathway in physio-pathological contexts compatible with the activation of inflammatory pathways -Human ferritins engineered for selective drug transport -Development of EPR methods for the study of proteins and radicals in cells
During the
SECOND YEAR OF THE COURSE, the 36th cycle doctoral students regularly attended the seminars and activities organized by teaching board and the BeMM Doctoral School and continued the experimental activities relating to their thesis project. Furthermore, they participated in conferences (mainly organized remotely) relevant to their lines of research, listed below: -Structural biology for the synergistic development of innovative drugs (BIOSTRUCT-FARM) -Slippery memory: how olive oil polyphenols could spare us from neurodegeneration -Understanding the mechanisms of folding and binding of MATH and SPRY domains -The role of BVR-A in the development of brain insulin resistance -STAT3 mediated carcinogenesis and influence of organochlorine environmental pollutants on chemoresistance -Role of Gasotransmitters in energy metabolism and in bacterial resistance to nitrosative stress -In silico design and evaluation of exon skipping-inducing antisense oligonucleotides for therapeutic intervention in rare cancers -Study of the interactions between macromolecules by bio-layer interferometry and turbidimetry -The characterization of Frog Skin-derived antimicrobial peptides for the development of new anti-infective agents with expanding properties
During the
THIRD YEAR OF THE COURSE, the 35th cycle doctoral students attended the lessons and seminars organized by teaching board and the BeMM Doctoral School and concluded the experimental activities relating to the thesis project. They did it to the drafting of the final thesis and all the theses were uploaded to the doctoral office website. The titles of the theses are as follows: -Structural bioinformatics analysis of the SARS-COV-2 proteome evolution to characterize the emerging variants of the virus and to suggest possible therapeutic strategies -Biocatalytic Applications of Lathyrus ccera amine oxidase -PARP-1 and Alzheimer’s disease: from molecular functions to biological outcomes in Drosophila melanogaster models -From root extract to antiarthritic facts: trip across a novel therapeutic approach to inflammation and pain -Comparison of the folding and the binding features of domains in isolation and in a multidomain construct -L-Arginine in Pseudomonas Aeruginosa controls c-di-GMP levels and biofilm formation