Over the last decade, cryo-electron microscopy (cryo-EM) has stepped up as the mainstream technology for studying the structure of cells, viruses and protein complexes at molecular resolution. In particular, single-particle electron microscopy has the ability to unravel the three-dimensional structure of biological molecules and assemblies by imaging non-crystalline specimens. The Cryo-EM Laboratory at University of Milan focuses on the architectural characterization of macromolecular complexes as targets for a deeper understanding of the molecular bases of both diseases and physiological pathways. In this talk, theoretical and practical aspects of single-particle EM will be addressed together with “real life” examples. In particular, the talk will cover the architectural characterization of (i) the anti-CRISPR protein AcrIIA6 from virulent streptococcal phage, (ii) the Azospirillum brasilense Glutamate Synthase in its different oligomeric assemblies, and (iii) cardiac amyloid fibrils from an immunoglobulin light chain (AL) amyloidosis patient.
May 15 2020, 12 am, online
Dr. Paolo Swuec Department of BioSciences University of Milan The link will be published on this site on the morning of May 15th
https://meet.google.com/gbs-swyw-hxp