RESEARCH FUNDS
Additional research funds (ML Euro) are made available in addition to those of Sapienza, assigned to the supervisors and tutors of doctoral students, including specific funds relating to international agreements or networks.
In particular, we highlight funds available on the following topics relevant to all doctoral curricula:
(Curriculum 1) antimicrobial resistance, infectious diseases including emerging ones, RNA viruses, retroviruses and microbiota.
(Curriculum 2) RNA:protein interactions, structure and inhibition of proteins, drug design, NADPH oxidase and ROS.
(Curriculum 3) genomic stability, mechanisms of cardiovascular damage, aggregation pathologies/Alzheimer's, neuromuscular pathologies, liver tumors, metastases and exosomes, neuroblastoma, cellular models and organoids.
The main ACTIVE FUNDED PROJECTS are indicated below.
The funding was obtained as part of competitive calls, the main ones of which are listed below, grouped by type of call/financing body.
PRIN_ MUR 2022: 11 projects financed.
PNRR: 7 projects funded within the National Centers (CN3- RNA Therapies), Extended Partnerships (PE7, PE13) and Rome Technopole (Spoke1), PNRR-Health Chronic Non-Communicable Diseases
MINISTRY OF HEALTH Finalized Research: 3 projects (2019 call and 2021 call)
LAZIO REGION: 2 projects funded by 2020 call.
FOUNDATIONS OR PRIVATE RESEARCH INSTITUTIONS: 5 AIRC Projects, 2 Telethons, 1 AriSLA, 2 Pasteur Institute Cenci Bolognetti Foundation, 2 Italian Institute of Technology, 1 MDA on Muscular Dystrophy.
EUROPEAN PROJECTS: 1 project.
SPACES AVAILABLE FOR PHD STUDENTS
Doctoral students are always assigned a dedicated work space in the individual laboratories of their supervisors and tutors. In the Departments to which the individual supervisors belong, there are also common rooms dedicated to doctoral students (some examples given below).
Department of Biochemical Sciences (Building CU027) has a room with workstations available for doctoral students and post-docs working in the Department, as well as a reading room in building CU010.
Dept. of Medical and Surgical Sciences and Biotechnology has a common student study room open from 8am to 6.30pm.
Building RM026 Viale di Porta Tiburtina 28, doctoral students are assigned a dedicated work space in the laboratories on the ground floor.
Department of Public Health and Infectious Diseases (building CU015) Microbiology Section, a room with workstations is available for doctoral students working in the Section. Department of Biology and Biotechnology, Institute of Genetics (CU022) all PhD students have a desk in a shared study room (third floor).
Laboratories and specialized equipment available in the individual Departments are described in detail in the section SCIENTIFIC EQUIPMENT.
SCIENTIFIC EQUIPMENT.
Specialized hardware and software equipment. A computerized classroom is available in the Department of Biochemical Sciences equipped with 15 computing stations, dedicated to advanced computing and also available for frontal and tutorial teaching activities of the doctorate.
Computers are also available for advanced calculation in the fields of bioinformatics and structural biology with some dedicated programs such as: Pymol 2.5, Pymod 3, DockingPie 1.2 Python 3, MVD 7.0, Vina, Autodock Gromacs, Amber,CCP4 suite (Coot) Phenix, Chimera Relion Cryospark
It is also noted:
- Access to High Performance computing through CINECA. This method is particularly important for the analysis of complex data (Big Data), a line of research active within our Doctorate.
- Access to the major biological databases (publicly available): students are instructed on their use and methods of access.
Main scientific equipment: In the research laboratories to which the doctoral students belong, the basic equipment for carrying out the experimental activities is available. Here we highlight some particularly important equipment present in the departments of the supervisors, for which they are responsible or which doctoral students can access. Whenever possible, the web addresses of the individual platforms/or laboratories in which they are present have been indicated.
2 Oxford Nanopore Technologies genomic sequencers type MK1C
Lifereal Nanoready (Nanodrop)
FastGene B/G FASBOX Nippon Genetics
QIAcuity One 5-PLEX Device (Digital PCR) QIAGEN,
Microscopy Facility (Confocal, Time Lapse Microscopy etc... in Via dei Sardi). https://research.uniroma1.it/laboratorio/193436
Facility Rarity (X-ray and Gravity simulator) https://research.uniroma1.it/laboratorio/192411
https://research.uniroma1.it/laboratorio/192452
VEVO 3100 VISUALSONIC ultrasound system. https://research.uniroma1.it/laboratorio/145227.
HYP-ACB https://research.uniroma1.it/laboratorio/144671#/0
PROFACT https://research.uniroma1.it/laboratorio/145145#/0
ARP https://research.uniroma1.it/laboratorio/144295
https://research.uniroma1.it/laboratorio/145238
Spinning disk confocal microscope; Utlima 2P Plus multiphoton microscope; 2 patch clamp setups; 2 calcium imaging setups; 1 multielectrode array
https://research.uniroma1.it/laboratorio/16382
BD FACSAria III for cell sorting, BD FACSCanto II for flow cytometric analysis and BD FACSymphony for multiparametric analyzes (up to 30 colors),
BD Rhapsody System for transcriptome analysis at the single cell level,
Illumina HiSeq1500 next-generation sequencing (NGS) platform, Thermo Scientific Orbitrap Fusion Tribid mass spectrometer for proteomics and metabolomics analysis, Bio-Plex MAGIX multiplex reader, Leica EG1150 H heated paraffin embedding module and microtome semi-automatic Leica RM2245, a Zeiss Axio Scope A.1 fluorescence microscope supported by the AxioCam 208 camera, a ZEISS spectral confocal microscope on an inverted stand Model LSM 980 implemented with an Airyscan2 super resolution system for fast live-imaging and time lapse and with the AxioObserver 7 software.
Celldiscoverer 7 confocal microscope, Carl Zeiss AG; Q Exactive plusTM Hybrid Quadrupole-OrbitrapTM Mass Spectrometer (Thermo Fisher Scientific);
Equipment at Spallanzani INMI: Celldiscoverer 7 confocal microscope, Carl Zeiss AG; EXOID IZON microvesicle analyzer; Q Exactive plusTM Hybrid Quadrupole-OrbitrapTM Mass Spectrometer (Thermo Fisher Scientific)
https://research.uniroma1.it/laboratorio/145303#/0
FLUORIMETER: CytoFLEX V5/B5/R3 (13 Detectors, 3 Lasers) - Beckman Coulter
DIGITAL PCR: Digital Qiacuity One, 5-Plex Device - QIAGEN
CHEMILUMINESCENCE: Chemidoc Image System - BioRad
P2 cell room at CU026. The research facilities within the CLN2S (https://n4n.iit.it/facilities) can be used by doctoral students with an IIT scholarship.
Conventional flow cytometers: BD LSR Fortessa (13 colors) and FACSCAnto (6 colors). Access to the Cytek Aurora CS spectral sorter acquired with the University's Large Equipment Call.
UV Perkin-Elmer 552 Spectrophotometer, NMR Bruker AC400 Spectrometer,Thermo Scientific UltiMate 3000 UHPLC System,Thermo Scientific MSQ Plus Mass Spectrometer, CoronaVeo Charged Aerosol Detector,Buchi RII Rotavapors,Heidolph Rotavapors,PARR Hydrogenator,Stuart melting point apparatus, Biotage Initiator Microwave Reactor, Biotage Isolera One Flash Chromatography System,Perkin Elmer EnSight Multimode Plate Reader, ThalesNano H-Cube Pro Hydrogenation Flow Reactor, Thermo Scientific Q Exactive UHMR Hybrid Quadrupole-Orbitrap Mass Spectrometer
LIBRARIES: the real and virtual libraries of Sapienza and all the others are accessible to students
associated offices
ASSOCIATED HEALTHCARE FACILITIES: The research activity of some doctoral students takes place within facilities with healthcare aspects, such as: Policlinico Umberto I
INMI Lazzaro Spallanzani,
IRCCS Neuromed
ICOT Latina
S.Maria Goretti Latina Hospital Bambino Gesù Pediatric Hospital
JOURNAL SUBSCRIPTIONS: Access to major online scientific journals