Three-dimensional (3D) bioprinting has revolutionized the field of tissue engineering and
regenerative medicine (TERM), offering the ability to fabricate tissues with high precision.
Despite this progress, replicating the complex structure and cellular diversity of native
tissues remains a challenge. Traditional 3D bioprinting methods often face limitations in
incorporating multiple cell types and biomaterials while maintaining fine spatial control over
cellular environments. Furthermore, these methods typically lack the ability to modulate
cellular behavior prior to deposition, restricting tissue maturation and functional outcomes.
We are working on advanced 3D bioprinting strategies leveraging microfluidic-assisted
technologies to precisely compartmentalize biomaterials, growth factors and cells, enabling
the fabrication of complex tissue constructs with functional gradients. Unconventional
microfluidic printheads integrated with flow-focusing and passive mixer modules can be
used to dynamically control fiber diameter, biomaterial composition, and cell distribution.
This innovation supports the creation of compartmentalized 3D layers with tailored cell
densities, mimicking the intricate architecture of native tissues and their interfaces.
Harnessing microfluidic-assisted 3D bioprinting, we can engineer constructs that can closely
mimic patient-specific districts with bioengineering platforms that use planar and
unconventional deposition approaches. Our versatile system not only enables the
generation of physiologically relevant tissue substitutes but also offers a powerful platform
for disease modeling and drug development through the recreation of diverse cellular
microenvironments within a single construct. By overcoming the inherent limitations of
conventional bioprinting techniques, this method holds immense potential for advancing
TERM applications, providing novel solutions for aging-related tissue degeneration and improving therapeutic strategies.
21/03/2025 at 12.00 aula Ulrico Bracci (Policlinico Umberto I) Dott. Gianluca Cidonio Department of Mechanical and Aerospace Engineering University of Rome La Sapienza