YURI PROZZILLO

Dottore di ricerca

ciclo: XXXV



Titolo della tesi: Non-canonical role of chromatin remodelling complexes: lessons from SRCAP ATPase

Chromatin is a highly dynamic nucleoprotein structure which organization is finely regulated to ensure the balance between packaging and accessibility of the eukaryotic genome. Such regulation is mainly mediated by covalent histone modifiers and chromatin remodeling complexes (CRCs). Four families of ATP-dependent CRCs are currently known (INO80, SWI/SNF, ISWI, CHD). Among them the human INO80 family includes the SRCAP and Tip60/p400 complexes. The main function of these two complexes is to promote the exchange of the canonical histone H2A with the histone H2A variants (H2A.X, H2A.Z). The results of two recently papers, published by our group, support the existence of an evolutionarily conserved phenomenon in which the subunits of SRCAP and Tip60/p400 complexes relocate from chromatin to mitotic apparatus, playing a role in human and Drosophila cell division. In this context, my PhD project is aimed to deepen our understanding of the extra-chromatin roles played by the SRCAP subunit and to get mechanistic insight on the molecular aspects of this multi-functional ATPase and on its interaction network during mitosis and cytokinesis. Moreover, because Srcap gene dominant mutations are known to cause the Floating-Harbor developmental syndrome (FHS), it was also analysed the progression of cell division in one lymphoblastoid cell line derived from a FHS patient. The results of my work suggested that SRCAP may be involved in: i) promoting mitotic spindle dynamics by ensuring microtubules polymerization and/or stabilization, as a new microtubule associated protein (MAP), and ii) contributing to secure the final cut necessary for proper execution of abscission during cytokinesis. Finally, these results suggested that, in addition to the chromatin regulation defects, a misfunctional SRCAP ATPase causes cell division defects that contribute to the onset of the developmental abnormalities characteristic of FHS.

Produzione scientifica

11573/1663238 - 2023 - Epigenetic silencing of P-element reporter genes induced by transcriptionally active domains of constitutive heterochromatin in Drosophila melanogaster
Messina, Giovanni; Celauro, Emanuele; Massimiliano Marsano, Renè; Prozzillo, Yuri; Dimitri, Patrizio - 01a Articolo in rivista
rivista: GENES (Basel : Molecular Diversity Preservation International) pp. 12- - issn: 2073-4425 - wos: WOS:000915022800001 (3) - scopus: 2-s2.0-85146777640 (3)

11573/1679303 - 2023 - Knockdown of DOM/Tip60 complex subunits impairs male meiosis of Drosophila melanogaster
Prozzillo, Yuri; Fattorini, Gaia; Ferreri, Diego; Leo, Manuela; Dimitri, Patrizio; Messina, Giovanni - 01a Articolo in rivista
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. - - issn: 2073-4409 - wos: WOS:000997355800001 (3) - scopus: 2-s2.0-85160620364 (3)

11573/1693460 - 2023 - Unconventional roles of chromatin remodelers and long non-coding RNAs in cell division
Prozzillo, Yuri; Santopietro, Maria Virginia; Messina, Giovanni; Dimitri, Patrizio - 01g Articolo di rassegna (Review)
rivista: CELLULAR AND MOLECULAR LIFE SCIENCES (Basel: Birkhäuser 1997-) pp. - - issn: 1420-9071 - wos: WOS:001104924100002 (0) - scopus: 2-s2.0-85177187164 (0)

11573/1656716 - 2022 - The green valley of Drosophila melanogaster constitutive heterochromatin: protein-coding genes involved in cell division control
Messina, Giovanni; Prozzillo, Yuri; Bizzochi, Greta; Massimiliano Marsano, Renè; Dimitri, Patrizio - 01g Articolo di rassegna (Review)
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. - - issn: 2073-4409 - wos: WOS:000868028500001 (2) - scopus: 2-s2.0-85139783403 (2)

11573/1651744 - 2022 - Evolutionary conserved relocation of chromatin remodeling complexes to the mitotic apparatus
Messina, Giovanni; Prozzillo, Yuri; Delle Monache, Francesca; Santopietro, Maria Virginia; Dimitri, Patrizio - 01a Articolo in rivista
rivista: BMC BIOLOGY (London : BioMed Central) pp. 1-21 - issn: 1741-7007 - wos: WOS:000835708700001 (6) - scopus: 2-s2.0-85135351885 (7)

11573/1567844 - 2021 - The ATPase SRCAP is associated with the mitotic apparatus, uncovering novel molecular aspects of Floating-Harbor syndrome
Messina, Giovanni; Prozzillo, Yuri; Delle Monache, Francesca; Santopietro, Maria Virginia; Atterrato, Maria Teresa; Dimitri, Patrizio - 01a Articolo in rivista
rivista: BMC BIOLOGY (London : BioMed Central) pp. - - issn: 1741-7007 - wos: WOS:000694234400002 (10) - scopus: 2-s2.0-85114364487 (12)

11573/1546026 - 2021 - In vivo silencing of genes coding for dTip60 chromatin remodeling complex subunits affects polytene chromosome organization and proper development in Drosophila melanogaster
Prozzillo, Y.; Cuticone, S.; Ferreri, D.; Fattorini, G.; Messina, G.; Dimitri, P. - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel (Matthaeustrasse 11) : Molecular Diversity Preservation International MDPI) pp. 1-15 - issn: 1661-6596 - wos: WOS:000650414000001 (6) - scopus: 2-s2.0-85104674127 (6)

11573/1502855 - 2021 - droTAG: adapting dTAG toolkit to Drosophila melanogaster
Prozzillo, Yuri; Messina, Giovanni - 01f Lettera, Nota
rivista: AMERICAN JOURNAL OF BIOMEDICAL SCIENCE & RESEARCH (Rancho Cucamonga CA: BiomedGrid LLC) pp. 368-369 - issn: 2642-1747 - wos: (0) - scopus: (0)

11573/1657143 - 2020 - Targeted protein degradation tools: overview and future perspectives
Prozzillo, Yuri; Fattorini, Gaia; Santopietro, Maria Virginia; Suglia, Luigi; Ruggiero, Alessandra; Ferreri, Diego; Messina, Giovanni - 01g Articolo di rassegna (Review)
rivista: BIOLOGY (Basel : MDPI) pp. 1-15 - issn: 2079-7737 - wos: WOS:000602249600001 (26) - scopus: 2-s2.0-85096663235 (29)

11573/1313151 - 2019 - The true story of Yeti, the "abominable" heterochromatic gene of drosophila melanogaster
Prozzillo, Yuri; Delle Monache, Francesca; Ferreri, Diego; Cuticone, Stefano; Dimitri, Patrizio; Messina, Giovanni - 01g Articolo di rassegna (Review)
rivista: FRONTIERS IN PHYSIOLOGY (Lausanne: Frontiers Research Foundation, 2010-) pp. 1-9 - issn: 1664-042X - wos: WOS:000482203700001 (10) - scopus: 2-s2.0-85072219122 (10)

11573/957437 - 2017 - The human Cranio Facial Development Protein 1 (Cfdp1) gene encodes a protein required for the maintenance of higher-order chromatin organization
Messina, Giovanni; Atterrato, Maria Teresa; Prozzillo, Yuri; Piacentini, Lucia; Losada, Ana; Dimitri, Patrizio - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. - - issn: 2045-2322 - wos: WOS:000398105500001 (18) - scopus: 2-s2.0-85016996197 (21)

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma