MAILA CHIRIVI'

PhD Graduate

PhD program:: XXXVI


supervisor: Prof.ssa Saula Checquolo
co-supervisor: Dr.ssa Claudia Bearzi

Thesis title: Role of the extracellular matrix in human pathophysiologies

The extracellular matrix (ECM) is a complex network of fibers composed mainly of structural and fibrous proteins such as collagens and proteoglycans, which give it strength and plasticity. Both the composition of the ECM and the molecular structure are significantly modified during the repair of damaged tissues as well as in the progression of various diseases such as tumors and dystrophies. Recently many researches have attributed to the ECM features, the responsibility of modifying the transcriptomic profile of entire physiological systems, in pathological conditions. The development of advanced technologies applied to medicine, supported by innovative biomaterials, can provide significant insights into the knowledge of pathologies, recapitulating human three-dimensional pathological systems in vitro, included in a pathological ECM. Recently, it has been demonstrated that in triple negative breast cancer (TNBC) both the molecular and physical components of the tumor ECM are able to inhibit the permeabilization of the tumor mass by cytotoxic CD8+ T cells. Therefore, the main objective of the first part of the project was to create an investigation tool, recapitulating the physical characteristics of the tumor ECM in which to study the induced transcriptomic perturbation in human T lymphocytes. Our data showed that it is sufficient to increase the stiffness of the ECM, recapitulating the tumor microenvironment, to induce the physical, functional and molecular remodeling of CD8 T lymphocytes, significantly altering their immune belligerence. The second part of this work concerned the generation of a bio-printable natural hydrogel, derived from decellularized dystrophic porcine myocardium, to study the harmful cellular perturbations affecting the heart, in Duchenne Muscular Dystrophy (DMD). To this end, for the first time, we generated a pathological bio-printable hydrogel, which recapitulates the molecular composition and physical properties characteristic of the dystrophic cardiac ECM. Then, fibroblasts, induced pluripotent stem cells (iPSC)-derived cardiomyocytes and embryonic stem cells (ESC)-derived neurons were used to validate the functionality of the DMD bio-ink, demonstrating how the composition of the ECM is sufficient to induce molecular remodeling in cells. The need to investigate the role of the ECM in a pathological context led this study to develop revolutionary in vitro models, useful to overcome suboptimal translational models of ECM remodeling which could also be useful for testing the efficacy and toxicity of new drugs.

Research products

11573/1671988 - 2023 - The protein network in subcutaneous fat biopsies from patients with AL amyloidosis: more than diagnosis?
Silvestre, Dario Di; Brambilla, Francesca; Lavatelli, Francesca; Chirivì, Maila; Canetti, Diana; Bearzi, Claudia; Rizzi, Roberto; Bijzet, Johan; Hazenberg, Bouke P. C.; Bellotti, Vittorio; Gillmore, Julian D.; Mauri, Pierluigi - 01a Articolo in rivista
paper: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. 699- - issn: 2073-4409 - wos: WOS:000947560600001 (2) - scopus: 2-s2.0-85149778042 (2)

11573/1652010 - 2022 - Human platelet lysate‐derived extracellular vesicles enhance angiogenesis through miR‐126
Bordin, Antonella; Chirivì, Maila; Pagano, Francesca; Milan, Marika; Iuliano, Marco; Scaccia, Eleonora; Fortunato, Orazio; Mangino, Giorgio; Dhori, Xhulio; De Marinis, Elisabetta; D'amico, Alessandra; Miglietta, Selenia; Picchio, Vittorio; Rizzi, Roberto; Romeo, Giovanna; Pulcinelli, Fabio; Chimenti, Isotta; Frati, Giacomo; De Falco, Elena - 01a Articolo in rivista
paper: CELL PROLIFERATION ([Oxford]: [Blackwell Science].) pp. 1-19 - issn: 1365-2184 - wos: WOS:000840138700001 (14) - scopus: 2-s2.0-85135740211 (14)

11573/1652533 - 2022 - Biomimetic keratin-coated gold nanoparticles for photo-thermal therapy in a 3D bioprinted glioblastoma tumor model
Chirivì, Maila; Bearzi, Claudia; Rosa, Paolo; Miglietta, Selenia; Petronella, Francesca; De Falco, Elena; Calogero, Antonella; Pani, Roberto; Petrozza, Vincenzo; Perotto, Giovanni; Rizzi, Roberto; De Sio, Luciano - 01a Articolo in rivista
paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 1-15 - issn: 1422-0067 - wos: WOS:000851229800001 (11) - scopus: 2-s2.0-85137915098 (17)

11573/1651009 - 2022 - Unusual Association of NF-κB Components in Tumor-Associated Macrophages (TAMs) Promotes HSPG2-Mediated Immune-Escaping Mechanism in Breast Cancer
De Paolis, Veronica; Maiullari, Fabio; Chirivi', Maila; Milan, Marika; Cordiglieri, Chiara; Pagano, Francesca; Rita La Manna, Alessandra; De Falco, Elena; Bearzi, Claudia; Rizzi, Roberto; Parisi, Chiara - 01a Articolo in rivista
paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:000832333900001 (7) - scopus: 2-s2.0-85135134685 (8)

11573/1649263 - 2022 - Glucocorticoid receptor antagonization propels endogenous cardiomyocyte proliferation and cardiac regeneration
Pianca  And Francesca Sacchi , Nicola; Baruch Umansky, Kfir; Chirivi', Maila; Iommarini, Luisa; Da Pra , Silvia; Papa , Valentina; Bongiovanni, Chiara; Miano , Carmen; Pontis , Francesca; Braga, Luca; Tassinari, Riccardo; Pantano, Elvira; Shastry Patnala, Rahul; Mazzeschi, Martina; Cenacchi , Giovanna; Maria Porcelli, Anna; Lauriola, Mattia; Ventura, Carlo; Giacca , Mauro; Rizzi, Roberto; Tzahor  And Gabriele D’Uva  , Eldad - 01a Articolo in rivista
paper: NATURE CARDIOVASCULAR RESEARCH (New York NY: Nature Portfolio, 2021-) pp. 617-633 - issn: 2731-0590 - wos: WOS:001126672400010 (14) - scopus: 2-s2.0-85134337640 (18)

11573/1578651 - 2021 - Focus on the road to modelling cardiomyopathy in muscular dystrophy
Canonico, Francesco; Chirivi, Maila; Maiullari, Fabio; Milan, Marika; Rizzi, Roberto; Arcudi, Alessandra; Galli, Mattia; Pane, Marika; Gowran, Aoife; Pompilio, Giulio; Mercuri, Eugenio; Crea, Filippo; Bearzi, Claudia; D'amario, Domenico - 01g Articolo di rassegna (Review)
paper: CARDIOVASCULAR RESEARCH (editore attuale: ELSEVIER SCIENCE BV, PO BOX 211, AMSTERDAM, NETHERLANDS, 1000 AE primo editore: BMJ / British Medical Journal Publishing Group:PO Box 299, London WC1H 9TD United Kingdom:011 44 20 73836270, EMAIL: subscriptions@bmjgroup.com, INTERNET: http://www.bmjpg.com/bmj, Fax: 011 44 20 73836402) pp. 1-13 - issn: 0008-6363 - wos: WOS:000756330200001 (1) - scopus: 2-s2.0-85133144086 (2)

11573/1551913 - 2021 - Tumor Extracellular Matrix Stiffness Promptly Modulates the Phenotype and Gene Expression of Infiltrating T Lymphocytes
Chirivì, Maila; Maiullari, Fabio; Milan, Marika; Presutti, Dario; Cordiglieri, Chiara; Crosti, Mariacristina; Sarnicola, Maria Lucia; Soluri, Andrea; Volpi, Marina; Święszkowski, Wojciech; Prati, Daniele; Rizzi, Marta; Costantini, Marco; Seliktar, Dror; Parisi, Chiara; Bearzi, Claudia; Rizzi, Roberto - 01a Articolo in rivista
paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 1-19 - issn: 1422-0067 - wos: WOS:000660171700001 (35) - scopus: 2-s2.0-85106730717 (39)

11573/1549281 - 2021 - In vivo organized neovascularization induced by 3D bioprinted endothelial-derived extracellular vesicles
Maiullari, F.; Chirivi, Maila.; Costantini, M.; Ferretti, A. M.; Recchia, S.; Maiullari, S.; Milan, M.; Presutti, D.; Pace, V.; Raspa, M.; Scavizzi, F.; Massetti, M.; Petrella, L.; Fanelli, M.; Rizzi, M.; Fortunato, O.; Moretti, F.; Caradonna, E.; Bearzi, C.; Rizzi, R. - 01a Articolo in rivista
paper: BIOFABRICATION (Bristol : IOP Pub., 2009-) pp. - - issn: 1758-5082 - wos: WOS:000639512800001 (27) - scopus: 2-s2.0-85102252541 (28)

11573/1586594 - 2021 - A scalable, clinically severe pig model for Duchenne muscular dystrophy
Stirm, Michael; Fonteyne, Lina Marie; Shashikadze, Bachuki; Lindner, Magdalena; Chirivi, Maila; Lange, Andreas; Kaufhold, Clara; Mayer, Christian; Medugorac, Ivica; Kessler, Barbara; Kurome, Mayuko; Zakhartchenko, Valeri; Hinrichs, Arne; Kemter, Elisabeth; Krause, Sabine; Wanke, Rüdiger; Arnold, Georg J; Wess, Gerhard; Nagashima, Hiroshi; De Angelis, Martin Hrabĕ; Flenkenthaler, Florian; Kobelke, Levin Arne; Bearzi, Claudia; Rizzi, Roberto; Bähr, Andrea; Reese, Sven; Matiasek, Kaspar; Walter, Maggie C; Kupatt, Christian; Ziegler, Sibylle; Bartenstein, Peter; Fröhlich, Thomas; Klymiuk, Nikolai; Blutke, Andreas; Wolf, Eckhard - 01a Articolo in rivista
paper: DISEASE MODELS & MECHANISMS (Cambridge : Company of Biologists, 2007-) pp. - - issn: 1754-8403 - wos: WOS:000751637600011 (24) - scopus: 2-s2.0-85121987919 (25)

11573/1549285 - 2020 - Extracellular vesicles from skeletal muscle cells efficiently promote myogenesis in induced pluripotent stem cells
Baci, D.; Chirivi, Maila.; Pace, V.; Maiullari, F.; Milan, M.; Rampin, A.; Somma, P.; Presutti, D.; Garavelli, S.; Bruno, A.; Cannata, S.; Lanzuolo, C.; Gargioli, C.; Rizzi, R.; Bearzi, C. - 01a Articolo in rivista
paper: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. - - issn: 2073-4409 - wos: WOS:000550783300001 (14) - scopus: 2-s2.0-85087139439 (18)

11573/1173091 - 2018 - A multi-cellular 3D bioprinting approach for vascularized heart tissue engineering based on HUVECs and iPSC-derived cardiomyocytes
Maiullari, Fabio; Marcocostantini, ; Milan, Marika; Pace, Valentina; Chirivi', Maila; Maiullari, Silvia; Rainer, Alberto; Baci, Denisa; El-Sayed Marei, Hany; Seliktar, Dror; Gargioli, Cesare; Bearzi, Claudia; Rizzi, Roberto - 01a Articolo in rivista
paper: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. - - issn: 2045-2322 - wos: WOS:000444086700044 (238) - scopus: 2-s2.0-85053219620 (286)

11573/1020415 - 2018 - Givinostat reduces adverse cardiac remodeling regulating fibroblasts activation
Milan, Marika; Pace, Valentina; Maiullari, Fabio; Chirivi', Maila; Baci, Denisa; Maiullari, Silvia; Madaro, Luca; Maccari, Sonia; Stati, Tonino; Marano, Giuseppe; Frati, Giacomo; Lorenzo Puri, Pier; De Falco, Elena; Bearzi, Claudia; Rizzi, Roberto - 01a Articolo in rivista
paper: CELL DEATH & DISEASE (Nature Group) pp. 1-17 - issn: 2041-4889 - wos: WOS:000427386000002 (31) - scopus: 2-s2.0-85041100911 (38)

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