1/11/2023- on going
PhD student in Network Oncology and Precision Medicine 39 cycle
Laboratory of Tumor Immunology and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza
WORKING EXPERIENCE
INTERNSHIP
Master's degree in Medical Biotechnology.
Laboratory of Tumor Immunology and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza
Master's degree in Medical Biotechnology.
“A.Gulino” Molecular Oncology Laboratory
Department of Molecular Medicine, University of Rome La Sapienza
Three-year degree in Biotechnology.
Laboratory of Human and Population Genomics
“Charles Darwin” Department of Biology and Biotechnology, University of Rome La Sapienza
EDUCATION AND TRAINING
January 2024
Certificate of training "Preclinical Testing and Animal Welfare", Sapienza University of Rome
27/10/2023
Master's Degree in Medical Biotechnology (LM-9). University of Rome, La Sapienza
Experimental Thesis title: "CD137+ lymphocytes as biomarkers of response to anti-PD1 therapy in patients with NSCLC", at laboratory of Tumor Immunology and Cell Therapies.
March 2023- June 2023
CIVIS Program in ''Technical Innovation: Application to Immuno-Oncology'' University of Rome La Sapienza.
1 week internship at the experimental Histo-pathology laboratory (prof. Emanuelle Caraffe), CRCM, University of Marseille.
19/10/2021
Bachelor's Degree in Biotechnology (L-2). University of Rome, La Sapienza.
Experimental Thesis title: ''Evaluation of the discrimination power of rapidly mutating Y chromosome microsatellites"
2018
Secondary School Diploma
Amedeo Avogadro Scientific High School, Rome.
LABORATORY SKILLS
-Flow cytometry
-Isolation of PBMCs from blood of cancer patients by gradient centrifugation (Ficoll-Paque)
-Immunophenotyping of T cells, T Reg, exhausted T, myeloid suppressor cells (MDSC) by flow cytometry (Dx Flex, FACS Canto)
-Cell cultures (primary cultures and cell lines)
-Western Blotting
-Separation and characterization of blood components (PBMC, serum, plasma)
-Analysis of FlowJo and Prism data
-Cell lysis and protein extraction
-PCR, RT-PCR, Real-Time PCR
-RNA extraction and purification
-ELISA and Luminex assay
-MTT essay
-Senescence assay with β galactosidase
SEMINARS
-16/11/23 flowJo course: "Beyond conventional data analysis"
-13/12/23 "The MyD88-adapter-like (MAL) functions in chronic inflammatory pathways" prof. Mirza Baig
-Soft skills 2023 edition
RESEARCH ACTIVITY
Research and evaluation of prognostic and predictive biomarkers in patients affected by non-small cell lung cancer, in the absence of gene mutations, undergoing immunotherapy.
PUBLICATIONS AND ABSTRACTS
4-6/12/23 35th AICC international meeting, Translational and precision medicine
Poster: “Circulating CD137+ T cell subsets and MDCSs as immune biomarkers for response to anti-PD1 immunotherapy in NSCLC patients” A. Asquino, L. Tuosto, F. Scirocchi, A. Pace, I.G. Zizzari, M. Siringo, E. Tramontano, E. Cortesi, M. Nuti, A. Rughetti, C. Napoletano.