Phd in NETWORK ONCOLOGY AND PRECISION MEDICINE

Education and training
27/10/2023: Master's Degree in Medical Biotechnology (LM-9), University of Rome La Sapienza. Experimental Thesis title: "CD137+ lymphocytes as biomarkers of response to anti-PD1 therapy in patients with NSCLC", at laboratory of Tumor Immunology and Cell Therapies.
19/10/2021: Bachelor's Degree in Biotechnology (L-2), University of Rome La Sapienza. Experimental Thesis title: ''Evaluation of the discrimination power of rapidly mutating Y chromosome microsatellites".
2018: Secondary School Diploma, Amedeo Avogadro Scientific High School, Rome.
February 2025 - July 2025: Civis programme "Immuno-Oncology in the era of spatial omics", Sapienza University of Rome, Italy. 1-week physical mobility at the CIML, University of Aix Marseille.
2024-2025: 2nd Level Master in Immuno Oncology and Cell Therapies (auditor), Department of Experimental Medicine, Sapienza University of Rome, Italy.
March 2024 - July 2024: Civis programme "Experimental Models in Molecular Biomedicine", Sapienza University of Rome, Italy. 1-week physical mobility at the UAM, Madrid.
May 2024: Course "Formazione generale in Salute e Sicurezza sul Lavoro (SSL) per lavoratori ed equiparati".
January 2024: Certificate of training "Preclinical Testing and Animal Welfare", Sapienza University of Rome.
March 2023 - June 2023: CIVIS Program ''Technical Innovation: Application to Immuno-Oncology'', University of Rome La Sapienza. 1 week internship at the experimental Histo-pathology laboratory (prof. Emanuelle Caraffe), CRCM, University of Marseille.

Work experience
1/11/2023 - on going: PhD student in Network Oncology and Precision Medicine, 39 cycle, Laboratory of Tumor Immunology and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza.
4/01/2026 - on going: Visiting PhD student at the Molecular Oncology Laboratory, Fundación de Investigación Hospital General Universitario de Valencia (Prof. Eloisa Jantus Lewintre), Valencia, Spain.

Internships
Master's degree in Medical Biotechnology: Laboratory of Tumor Immunology and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza.
Master's degree in Medical Biotechnology: “A.Gulino” Molecular Oncology Laboratory, Department of Molecular Medicine, University of Rome La Sapienza.
Three-year degree in Biotechnology: Laboratory of Human and Population Genomics “Charles Darwin”, Department of Biology and Biotechnology, University of Rome La Sapienza.

Laboratory skills
Flow cytometry; Isolation of PBMCs from blood of cancer patients by gradient centrifugation (Ficoll-Paque); Immunophenotyping of T cells, T Reg, exhausted T, myeloid suppressor cells (MDSC) by flow cytometry (Dx Flex, FACS Canto); Cell cultures (primary cultures, cell lines); Patient Derived Organoid (PDO); Western Blotting; Separation and characterization of blood components (PBMC, serum, plasma); Analysis of FlowJo and Prism data; Cell lysis and protein extraction; PCR, RT-PCR, Real-Time PCR; RNA extraction and purification; ELISA and Luminex assay; MTT essay; Senescence assay with β galactosidase.

Seminars
16/11/23: flowJo course "Beyond conventional data analysis".
13/12/23: "The MyD88-adapter-like (MAL) functions in chronic inflammatory pathways", prof. Mirza Baig.
Soft skills edition 2023.
12-15/02/24: “Single cell transcriptomics", “Spatial omics and cancer”, “Human brain organoids”, Prof. Nikolaus Rajewsky, MDC Berlin.
3/04/24: “Overcoming the obstacles faced by neoantigen cancer vaccines”, Prof. Peter D. Katsikis.
9/04/24: “Novel nanotechnologies for biomedical applications”, Prof. Thanh Nguyen, UCL, London, UK.
31/10/2024: "Spatial Transcriptomics applied to tumorigenesis and exposomes", Prof. Nikolaus Rajewsky.
04/10/2024: Head of EU Medical affairs of Incyte, Dr. Luca Marini.
08/10/2024: "Building a better induced regulatory T cell: Pitfalls, Progress, and Potential", Prof.ssa Lisa M. Minter.
14/10/2024: "Towards Non-coding RNA Therapeutics", Prof. Frank Slack.
27/11/24: Congress "Immunità e Cancro: il punto di vista dei Giovani Ricercatori".
4/02/2025: “Advanced Technologies in Single Cell Omics” Course (FISV, University of Milan).
05/2025: “Tumorigenesis in space and time”; “Spatial Transcriptomics, Pathology, and some news about Microplastics”. Prof. Nikolaus Rajewsky; Dr. Tancredi Massimo Pentimalli.

Research activity
Research and evaluation of prognostic and predictive biomarkers in patients with non-small cell lung cancer, in the absence of gene mutations, undergoing immunotherapy.