LIDIA CASTAGNETO GISSEY

Dottoressa di ricerca

ciclo: XXXIV


supervisore: PROF. GIOVANNI CASELLA

Titolo della tesi: Novel liquid biopsy tests for highly accurate diagnosis of NASH and liver fibrosis: a multicenter prospective cohort trial

Background: Approval of drugs for non-alcoholic steato-hepatitis (NASH) requires evidence of significant effects on histological endpoints, yet liver biopsy is an invasive procedure. Therefore, novel non-invasive biomarkers of NASH and/or liver fibrosis are needed. Methods: Proteomics was performed with iTRAQ and nano-LC-MS/MS, and flow-cytometry assay used to measure PLIN2 and RAB14 in peripheral blood CD14+CD16− monocytes. Caucasian patients with clinical risk factors for NASH and/or fibrosis without secondary causes, underwent percutaneous liver biopsy; 50 healthy subjects underwent needle liver biopsy during elective laparoscopic cholecystectomy (Training-cohort: 100 subjects; Validation-cohort: 150 subjects). Results: Monocyte PLIN2 and RAB14 were highly and scarcely expressed in NASH and liver fibrosis, respectively. Biomarker performance was compared with histology. Algorithm for NASH used PLIN2 mean-florescence-intensity (MFI) which, combined with triglyceride, ALT plasma levels and presence/absence of diabetes as covariates, showed an AUROC of 96% (CI: 93-100%) in the training and 98% (CI: 96-100%) in the validation cohort, with Accuracy: 91%, Sensitivity: 98%, Specificity: 85% in training and 96%, 94%, and 98% in validation cohort. Prediction of fibrosis (RAB14-MFI) used BMI, HDL-cholesterol, plasma glucose, and ALT levels as covariates, and yielded an AUROC of 95% (CI: 88-100%), with Accuracy: 95%, Sensitivity: 98% and Specificity: 87% in training and 97% (CI: 90-100%), 91%, 90%, and 93% in validation cohort. Novel biomarkers performed substantially better compared to the most widely used biomarkers (FIB4, NAFLD Fibrosis-Score, and AST-to-Platelet-ratio). Conclusions: Novel liquid biopsy test is highly accurate in diagnosing NASH and/or liver fibrosis, and is markedly more reliable than the most commonly used biomarkers, and on par with gold standard liver biopsy-based histology.

Produzione scientifica

  • 11573/832637 - 2016 - Changes in insulin sensitivity and secretion after sleeve gastrectomy (01a Articolo in rivista)
    CASELLA, GIOVANNI; SORICELLI, EMANUELE; CASTAGNETO GISSEY, LIDIA; REDLER, ADRIANO; BASSO, NICOLA
  • 11573/872982 - 2012 - Underestimation of urinary albumin to creatinine ratio in morbidly obese subjects due to high urinary creatinine excretion (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/872980 - 2012 - Insulin sensitivity and secretion modifications after bariatric surgery (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/872959 - 2014 - Insulin dynamics in young women with polycystic ovary syndrome and normal glucose tolerance across categories of body mass index (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/872971 - 2014 - Type 2 diabetes mellitus in 2013: A central role of the gut in glucose homeostasis (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/786071 - 2015 - Spider surgical system versus multiport laparoscopic surgery. Performance comparison on a surgical simulator (01a Articolo in rivista)
    GIANNOTTI, DOMENICO; CASELLA, GIOVANNI; PATRIZI, GREGORIO; DI ROCCO, GIORGIO; CASTAGNETO GISSEY, LIDIA; METERE, ALESSIO; BERNIERI, MARIA GIULIA; VESTRI, ANNA RITA; REDLER, ADRIANO
  • 11573/893112 - 2016 - Insulin resistance, microbiota, and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats (01a Articolo in rivista)
    BASSO, NICOLA; SORICELLI, EMANUELE; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/872983 - 2009 - Mechanisms of early improvement / resolution of type 2 diabetes after bariatric surgery (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/872987 - 2016 - Early effect of Roux-en-Y gastric bypass on insulin sensitivity and signaling. (01a Articolo in rivista)
    SEVERINO, ANNA; CASTAGNETO GISSEY, LIDIA
  • 11573/872977 - 2013 - Use of dicarboxylic acids in type 2 diabetes (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/1666233 - 2023 - Novel Modality of Endoluminal Anastomotic Integrity Assessment with Fluoroangiography After Left-sided Colorectal Resections (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; IODICE, ALESSANDRA; URCIUOLI, PAOLO; PONTONE, STEFANO; SALVATI, BRUNO; CASELLA, GIOVANNI
  • 11573/936676 - 2017 - Gastroesophageal reflux disease and Barrett's esophagus after laparoscopic sleeve gastrectomy. A possible, underestimated long-term complication (01a Articolo in rivista)
    GENCO, ALFREDO; SORICELLI, EMANUELE; CASELLA, GIOVANNI; MASELLI, ROBERTA; CASTAGNETO GISSEY, LIDIA; BASSO, NICOLA
  • 11573/1643037 - 2022 - Sex Difference in the Safety and Efficacy of Bariatric Procedures: a Systematic Review and Meta-analysis (01g Articolo di rassegna (Review))
    RISI, RENATA; TOZZI, ROSSELLA; MASI, DAVIDE; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI; GNESSI, LUCIO; WATANABE, MIKIKO
  • 11573/1675057 - 2023 - Author Spotlight: Lidia Castagneto‑Gissey (01m Editorial/Introduzione in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/1671847 - 2023 - The Role of Antibiotic Prophylaxis in Anastomotic Leak Prevention during Elective Colorectal Surgery: Systematic Review and Meta-Analysis of Randomized Controlled Trials (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; RUSSO, MARIA FRANCESCA; CASELLA MARIOLO, JAMES ROSSARIO; MARCELLINARO, ROSA; D'ANDREA, VITO; CARLINI, MASSIMO; CASELLA, GIOVANNI
  • 11573/1037674 - 2017 - Lipidomic changes in skeletal muscle in patients after biliopancreatic diversion (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO; CASELLA, GIOVANNI
  • 11573/1664434 - 2022 - Comment on: Alarmists at the Gates: Esophageal Adenocarcinoma After Sleeve Gastrectomy Is Not Diferent than with Other Bariatric/Metabolic Surgeries (01f Lettera, Nota)
    GENCO, ALFREDO; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/1664431 - 2022 - Upper gut heat shock proteins HSP70 and GRP78 promote insulin resistance, hyperglycemia, and non-alcoholic steatohepatitis (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; SALINARI, SERENELLA; BASSO, NICOLA; CASELLA, GIOVANNI
  • 11573/1680811 - 2022 - Correction: Advice of General Practitioner, of Surgeon, of Endocrinologist, and Self-Determination: the Italian Road to Bariatric Surgery (Obesity Surgery, (2022), 32, 6, (1996-2002), 10.1007/s11695-022-06042-8) (01b Commento, Erratum, Replica e simili)
    CASELLA, GIOVANNI; WATANABE, MIKIKO; LEONETTI, FRIDA; SILECCHIA, GIANFRANCO
  • 11573/1118553 - 2018 - The anorexigenic peptide neurotensin relates to insulin sensitivity in obese patients after BPD or RYGB metabolic surgery (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/1687830 - 2023 - Incidence of lymphomas in inflammatory bowel disease: report of an emblematic case, systematic review, and meta-analysis (01a Articolo in rivista)
    RUSSO, MARIA FRANCESCA; DIDDORO, ANNALISA; IODICE, ALESSANDRA; SEVERI, CAROLA; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/1664435 - 2023 - Accurate liquid biopsy for the diagnosis of non-alcoholic steatohepatitis and liver fibrosis (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; RUSSO, MARIA FRANCESCA; CASELLA, GIOVANNI; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1135494 - 2018 - 10-Year follow-up after laparoscopic sleeve gastrectomy. Outcomes in a monocentric series (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO; GENCO, ALFREDO; BASSO, NICOLA; CASELLA, GIOVANNI
  • 11573/1691255 - 2023 - Diverticular disease and oxidative stress: the link with mitochondria alterations in the different clinical phenotypes (01h Abstract in rivista)
    CAPPELLETTI, MARTINA; CASTAGNETO GISSEY, LIDIA; TARALLO, MARIARITA; PISANO, ANNALINDA; TATTOLI, IVAN; PALLOTTA, LUCIA; GIORDANO, CARLA; SEVERI, CAROLA
  • 11573/1682219 - 2023 - Bariatric-metabolic surgery versus lifestyle intervention plus best medical care in non-alcoholic steatohepatitis (BRAVES). a multicentre, open-label, randomised trial (01a Articolo in rivista)
    PANUNZI, SIMONA; CASTAGNETO GISSEY, LIDIA; POMPILI, MAURIZIO; CASELLA, GIOVANNI; FIORI, ENRICO
  • 11573/1679094 - 2023 - T.02.5 OXIDATIVE STRESS IN DIVERTICULAR DISEASE: MITOCHONDRIA INVOLVEMENT OF IN THE DIFFERENT CLINICAL PHENOTYPES (04f Poster)
    CAPPELLETTI, MARTINA; PISANO, ANNALINDA; CASTAGNETO GISSEY, LIDIA; TARALLO, MARIARITA; GIORDANO, CARLA; SEVERI, CAROLA; PALLOTTA, LUCIA
  • 11573/1196435 - 2017 - Massive pelvic recurrence of uterine leiomyomatosis with intracaval-intracardiac extension: video case report and literature review (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; MUSLEH, LAYLA; MENICONI, ROBERTO LUCA; RANOCCHI, FEDERICO
  • 11573/1675059 - 2023 - SICOB-endorsed national Delphi consensus on obesity treatment optimization: focus on diagnosis, pre-operative management, and weight regain/insufficient weight loss approach (01a Articolo in rivista)
    CASELLA, GIOVANNI; CASTAGNETO GISSEY, LIDIA
  • 11573/1675052 - 2020 - Publisher Correction: New insight into the mechanisms of ectopic fat deposition improvement after bariatric surgery (Scientific Reports, (2019), 9, 1, (17315), 10.1038/s41598-019-53702-4) (01b Commento, Erratum, Replica e simili)
    CASTAGNETO GISSEY, LIDIA; CERBELLI, BRUNA; SEVERINO, ANNA; BASSO, NICOLA; CASELLA, GIOVANNI
  • 11573/1664430 - 2022 - New Horizons: Emerging Antidiabetic Medications (01g Articolo di rassegna (Review))
    CASTAGNETO GISSEY, LIDIA
  • 11573/1675055 - 2023 - Y Not Roux‑en‑Y?: Resolution of Barrett’s Esophagus After Surgical Conversion of Gastric Sleeve in Two Patients (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; GUALTIERI, LOREDANA; DIDDORO, ANNALISA; LAURO, AUGUSTO; GENCO, ALFREDO; CASELLA, GIOVANNI
  • 11573/1337244 - 2018 - Intestinal peptide changes after bariatric and minimally invasive surgery: Relation to diabetes remission (01g Articolo di rassegna (Review))
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1337234 - 2019 - Simulation of gastric bypass effects on glucose metabolism and non-alcoholic fatty liver disease with the Sleeveballoon device (01a Articolo in rivista)
    CASELLA MARIOLO, JAMES ROSSARIO; CASTAGNETO GISSEY, LIDIA; ZOLI PORRONI, ALESSANDRO; RUBINO, FLAVIA; CASELLA, GIOVANNI
  • 11573/873058 - 2016 - How to choose the best metabolic procedure? (01g Articolo di rassegna (Review))
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1337264 - 2018 - Left hepatectomy with concomitant cavo-atrial and biliary tumor thrombectomy for invasive hepatocellular carcinoma: a video report (01m Editorial/Introduzione in rivista)
    CASTAGNETO GISSEY, LIDIA; MUSLEH, LAYLA; MENICONI, ROBERTO LUCA
  • 11573/873010 - 2016 - Obesity, type 2 diabetes, and the metabolic syndrome. Pathophysiologic relationships and guidelines for surgical intervention (01g Articolo di rassegna (Review))
    CASELLA MARIOLO, JAMES ROSSARIO; CASTAGNETO GISSEY, LIDIA
  • 11573/760289 - 2014 - Utilizzo del Seamguard nella sleeve gastrectomy laparoscopica: la nostra esperienza in 65 casi consecutivi (04d Abstract in atti di convegno)
    CASELLA, GIOVANNI; GIANNOTTI, DOMENICO; DI ROCCO, GIORGIO; PATRIZI, GREGORIO; CASTAGNETO GISSEY, LIDIA; REDLER, ADRIANO
  • 11573/1349946 - 2019 - Trattamento dietoterapico combinato con pallone intragastrico: un caso clinico incoraggiante. (04d Abstract in atti di convegno)
    ERNESTI, ILARIA; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI; LEONE, GIUSEPPE; GENCO, ALFREDO
  • 11573/1362265 - 2020 - The jejunum is the key factor in insulin resistance (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/1385933 - 2020 - Obesity surgery and cancer. What are the unanswered questions? (01g Articolo di rassegna (Review))
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO; CASELLA, GIOVANNI
  • 11573/1435816 - 2020 - Short­‐term weight­‐mediated effects of sleeve gastrectomy on echocardiographic surrogate markers of atherosclerotic vascular disease (01m Editorial/Introduzione in rivista)
    CASELLA, GIOVANNI; CASTAGNETO GISSEY, LIDIA
  • 11573/1435812 - 2020 - Sleeve gastrectomy and gastroesophageal reflux: a comprehensive endoscopic and pH-manometric prospective study (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; GENCO, ALFREDO; DEL CORPO, GIULIA; BADIALI, DANILO; CASELLA, GIOVANNI
  • 11573/1420438 - 2018 - Metabolic surgery and depression (02a Capitolo o Articolo)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1431441 - 2020 - Impact of COVID-19 outbreak on emergency surgery and emergency department admissions. An Italian level 2 emergency department experience (01f Lettera, Nota)
    CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI; DEL CORPO, GIULIA; IODICE, ALESSANDRA; LATTINA, ILARIO; FERRARI, PAOLO; IANNONE, IMMACOLATA; MINGOLI, ANDREA; LA TORRE, FILIPPO
  • 11573/1401999 - 2018 - Metabolic surgery; indications and outcomes (02a Capitolo o Articolo)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1421289 - 2019 - Roles of gut hormones in the regulation of food intake and body weight (02a Capitolo o Articolo)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO
  • 11573/1459431 - 2020 - The effect of standard versus longer intestinal bypass on glp-1 regulation and glucose metabolism in patients with type 2 diabetes undergoing roux-en-y gastric bypass. The long-limb study (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/1470343 - 2020 - Esophageal adenocarcinoma after sleeve gastrectomy. Actual or potential threat? Italian series and literature review (01a Articolo in rivista)
    GENCO, ALFREDO; CASTAGNETO GISSEY, LIDIA; ERNESTI, ILARIA; SORICELLI, EMANUELE; CASELLA, GIOVANNI
  • 11573/1457619 - 2021 - Small intestinal metabolism is central to whole-body insulin resistance (01a Articolo in rivista)
    SALINARI, SERENELLA; CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO; CASELLA, GIOVANNI
  • 11573/1457622 - 2020 - Duodenal-jejunal bypass improves nonalcoholic fatty liver disease independently of weight loss in rodents with diet-induced obesity (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CASELLA MARIOLO, JAMES ROSSARIO; STEFANIZZI, GIANLUIGI; CASELLA, GIOVANNI
  • 11573/1518014 - 2021 - Response to Sleeve gastrectomy may double the risk of esophageal adenocarcinoma in morbidly obese patients (01f Lettera, Nota)
    GENCO, ALFREDO; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI
  • 11573/1337222 - 2019 - New insight into the mechanisms of ectopic fat deposition improvement after bariatric surgery (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; DEL CORPO, GIULIA; GIORDANO, CARLA; CERBELLI, BRUNA; SEVERINO, ANNA; BASSO, NICOLA; GENCO, ALFREDO; CASELLA, GIOVANNI
  • 11573/1568187 - 2021 - GORD and Barrett’s oesophagus after bariatric procedures: multicentre prospective study (01a Articolo in rivista)
    GENCO, ALFREDO; CASTAGNETO GISSEY, LIDIA; GUALTIERI, LOREDANA; SORICELLI, EMANUELE; CASELLA, GIOVANNI
  • 11573/1550251 - 2021 - Repercussions of covid-19-related national lockdown on emergency surgery department: a longitudinal cohort monocentric study (01a Articolo in rivista)
    CASELLA, GIOVANNI; CASTAGNETO GISSEY, LIDIA; LATTINA, ILARIO; FERRARI, PAOLO; IODICE, ALESSANDRA; TESORI, MARIA CHIARA; CATANI, MARCO; ASSENZA, MARCO; MINGOLI, ANDREA; LA TORRE, FILIPPO
  • 11573/1628129 - 2022 - Metabolic/bariatric surgery protects against cardiovascular disease (01m Editorial/Introduzione in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/1628131 - 2022 - Perioperative management of acute pain by multimodal analgesia after laparoscopic sleeve gastrectomy: a prospective cohort study (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; FENGA, MARCO; RUSSO, MARIA FRANCESCA; CAPOANO, RAFFAELE; SALVATI, BRUNO; GENCO, ALFREDO; CASELLA, GIOVANNI
  • 11573/1628125 - 2022 - The early reduction of left ventricular mass after sleeve gastrectomy depends on the fall of branched-chain amino acid circulating levels (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; CAVARRETTA, ELENA; BASSO, NICOLA; CASELLA, GIOVANNI
  • 11573/1628135 - 2022 - Advice of general practitioner, of surgeon, of endocrinologist, and self‑determination: the italian road to bariatric surgery (01a Articolo in rivista)
    CASELLA, GIOVANNI; CASTAGNETO GISSEY, LIDIA; WATANABE, MIKIKO; LEONETTI, FRIDA
  • 11573/1650108 - 2022 - Intracholecystic versus intravenous indocyanine green (ICG) injection for biliary anatomy evaluation by fluorescent cholangiography during laparoscopic cholecystectomy: a case–control study (01a Articolo in rivista)
    CASTAGNETO GISSEY, LIDIA; RUSSO, MARIA FRANCESCA; IODICE, ALESSANDRA; CASELLA MARIOLO, JAMES ROSSARIO; PICCHETTO, ANDREA; D'AMBROSIO, GIANCARLO; URCIUOLI, IRENE; DE LUCA, ALESSANDRO; SALVATI, BRUNO; CASELLA, GIOVANNI
  • 11573/1664432 - 2022 - Editorial: Women in surgical oncology. 2021 (01m Editorial/Introduzione in rivista)
    CASTAGNETO GISSEY, LIDIA
  • 11573/1645877 - 2022 - OC.04.1 OXIDATIVE MUSCLE-DRIVEN ALTERATIONS: PARALLELISM BETWEEN UNCOMPLICATED AND COMPLICATED DIVERTICULAR DISEASE (04d Abstract in atti di convegno)
    CAPPELLETTI, MARTINA; GIOIA, ALESSIA; CASTAGNETO GISSEY, LIDIA; TARALLO, MARIARITA; PISANO, ANNALINDA; SEVERI, CAROLA; PALLOTTA, LUCIA
  • 11573/1467001 - 2020 - Effect of diet versus gastric bypass on metabolic function in diabetes (01b Commento, Erratum, Replica e simili)
    CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI

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