Research: A Multiomics Approach to Study Solid Tumor Malignancy
EDUCATION AND TRAINING
PhD Student in Network Oncology and Precision Medicine Sapienza University, Rome Department of Experimental Medicine Supervisor: Professor Agnese Po November 2024 – Present
Master's Degree in Genetics and Molecular Biology Sapienza University, Rome Thesis Title: "Identification of miR-302a-5p and miR-367-3p as diagnostic biomarkers and analysis of their biological role in prostate cancer" September 2021 – July 2024 Bachelor’s Degree in Biological Sciences University of Messina Thesis Title: "Fecal microbiota transplantation and its impact on obesity and metabolic syndrome" September 2017 – October 2021
PROFESSIONAL EXPERIENCE
Research Fellowship Sapienza University, Rome Department of Experimental Medicine Research Activity: "Role of unfolded protein response in medulloblastoma cancer stem cell maintenance: therapeutic implications and prognostic value" Supervisor: Professor Giuseppina Catanzaro February 2024 – August 2024
Master's Thesis Internship Master's Degree in Genetics and Molecular Biology Sapienza University, Rome Molecular Oncology Laboratory “Alberto Gulino,” Department of Molecular Medicine Supervisor: Professor Giuseppina Catanzaro October 2022 – July 2024
Training Internship Bachelor’s Degree in Biological Sciences University of Messina Papardo Hospital, Clinical Pathology and Virology Department September 2017 – November 2017
PERSONAL SKILLS
Native Language: Italian
Other: English B2
PROFESSIONAL SKILLS
Computer Skills: Windows Office Suite (Word, Excel, PowerPoint); Data analysis and statistical software (Image J, GraphPad Prism, PyMOL); Online databases (PubMed, PDB, UniProt). Cellular Biology: Growth and maintenance of commercial and primary cell cultures; development of primary cultures from tumor tissue and creation of 3D cellular models. Molecular Biology: Extraction and quantification of nucleic acids; RT-PCR; qPCR; Droplet Digital PCR; Transfection techniques; Purification of extracellular vesicles. Biochemistry: Protein extraction and quantification; Western Blotting; Immunofluorescence; Agarose gel electrophoresis for DNA analysis. Assays and Other Techniques: MTT cell viability assay; X-gal assay; Sample preparation for cell cycle analysis via flow cytometry; OCT embedding for histology; Immunofluorescence slide preparation; Hematoxylin/eosin staining on slides. ADDITIONAL INFORMATION Conferences and Seminars: - December 2–4, 2024: 36th AICC International Meeting 2024. "Circulating EV-MicroRNAs in Metastatic Melanoma as non-invasive biomarkers for Diagnosis and Treatment response." Authors: E. Splendiani, T. Noviello, A. Covre, C. Sabato, S. Cassandro, G. Gugliuzza, S. Coral, Z.M. Besharat, A. Po, A. Anichini, M. Maio, M. Ceccarelli, A. Di Giacomo, E. Ferretti. (Poster Presentation)