Phd in NETWORK ONCOLOGY AND PRECISION MEDICINE

Position

1/11/2023- today
PhD student in Network Oncology and Precision Medicine
Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza

WORK EXPERIENCE
Internship
Master's degree in Medical Biotechnology.
Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza

EDUCATION AND TRAINING

27/07/2023
Master's Degree in Medical Biotechnology (LM-9). University of Rome, La Sapienza
Experimental Thesis Title: ''Macrophage Galactose-type C lectin (MGL) and the carbohydrate ligand Tn as immunosuppression mediators in Glioblastoma patients''.

May 2023- June 2023
CIVIS programme in Technical Innovation: Application to Immuno- Oncology, Sapienza University Of Rome, Italy.
1-week internship at the Head of Antibody Therapeutics and Immunotargeting (ATI) Team of Patrick Chames, CRCM, University of Aix Marseille


20/10/2020
Bachelor's Degree in Biotechnology (L-2). University of Rome, La Sapienza.
Thesis Title: ''Pymol as a CADD Platform''

2017
High School Diploma
Isaac Newton Scientific High School ROME.

SPECIFIC SKILLS

-Cell cultures (primary cultures and cell lines).
-Western Blotting.
-Isolation of PBMC from human blood of cancer patients by gradient centrifugation (Ficoll-Paque).
-Freezing and thawing.
-Immunophenotyping of myeloid suppressor cells (MDSC), T Reg, T exhausted, T cells by flow cytometry (Dx Flex, FACS Canto).
-Data analysis with FlowJo and Prism.
-Cell lysis and protein extraction.
-Processing of tumor tissue of Glioblastoma (GB).
-Microvescicles isolation.
-ELISA essay.
-MTT essay.
-Senescence assay with β-Galactosidase.

Research Project
Investigating the molecular patterns of immunosuppression and immune exhaustion in Glioblastoma.

SEMINARS

-16/11/23 FlowJo Course: "Beyond conventional data analysis"

-13/12/23 "The MyD88-adaptor-like (MAL) functions in chronic inflammatory pathways" prof. Mirza Baig

-Soft skills 2023 edition