Position
1/11/2023- today
PhD student in Network Oncology and Precision Medicine
Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza
WORK EXPERIENCE
Internship
Master's degree in Medical Biotechnology.
Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza
EDUCATION AND TRAINING
27/07/2023
Master's Degree in Medical Biotechnology (LM-9). University of Rome, La Sapienza
Experimental Thesis Title: ''Macrophage Galactose-type C lectin (MGL) and the carbohydrate ligand Tn as immunosuppression mediators in Glioblastoma patients''.
May 2023- June 2023
CIVIS programme in Technical Innovation: Application to Immuno- Oncology, Sapienza University Of Rome, Italy.
1-week internship at the Head of Antibody Therapeutics and Immunotargeting (ATI) Team of Patrick Chames, CRCM, University of Aix Marseille
20/10/2020
Bachelor's Degree in Biotechnology (L-2). University of Rome, La Sapienza.
Thesis Title: ''Pymol as a CADD Platform''
2017
High School Diploma
Isaac Newton Scientific High School ROME.
SPECIFIC SKILLS
-Cell cultures (primary cultures and cell lines).
-Western Blotting.
-Isolation of PBMC from human blood of cancer patients by gradient centrifugation (Ficoll-Paque).
-Freezing and thawing.
-Immunophenotyping of myeloid suppressor cells (MDSC), T Reg, T exhausted, T cells by flow cytometry (Dx Flex, FACS Canto).
-Data analysis with FlowJo and Prism.
-Cell lysis and protein extraction.
-Processing of tumor tissue of Glioblastoma (GB).
-Microvescicles isolation.
-ELISA essay.
-MTT essay.
-Senescence assay with β-Galactosidase.
Research Project
Investigating the molecular patterns of immunosuppression and immune exhaustion in Glioblastoma.
SEMINARS
-16/11/23 FlowJo Course: "Beyond conventional data analysis"
-13/12/23 "The MyD88-adaptor-like (MAL) functions in chronic inflammatory pathways" prof. Mirza Baig
-Soft skills 2023 edition
-12/02/2024: Seminar “Single cell transcriptomics” Prof. Nikolaus Rajewsky MDC Berlin
-13/02/2024: Seminar “Spatial omics and cancer” Prof. Nikolaus Rajewsky MDC Berlin
-15/02/2024: Seminar “Human brain organoids” Prof. Nikolaus Rajewsky MDC Berlin
-02/21/2024: Seminar “The role of atypical chemokine receptors in inflammatory responses” Prof. Mattia LaLranchi
-9/04/2024: Seminar “Novel nanotechnologies for biomedical applications” Prof. Thanh Nguyen, UCL, London, UK
-3/04/2024: Seminar “Overcoming the obstacles faced by neoantigen cancer vaccines” Prof. Peter D. Katsikis
-31/10/2024 Spatial transcriptomics applied to tumorigenesis and expsosomes Prof.Nikolaus Rajewsky
04/10/2024 Head of EU Medical affairs of the pharmaceutical company Incyte Dr. Luca Marini
08/10/2024 Building a better induced regulatory T cell: Pitfalls, Progress, and Potential Prof. Lisa M.Minter
14/10/2024 Towards Non-coding RNA Therapeutics Prof. Frank Slack
PUBBLICATIONS AND ABSTRACTS
Gelibter A, Tuosto L, Asquino A, Siringo M, Sabatini A, Zizzari IG, Pace A, Scirocchi F, Valentino F, Bianchini S, Caponnetto S, Paoli D, Bellati F, Santini D, Nuti M, Rughetti A and Napoletano C (2024) Anti-PD1 therapies induce an early expansion of Ki67+CD8+ T cells in metastatic non-oncogene addicted NSCLC patients. Front. Immunol. 15:1483182. doi: 10.3389/fimmu.2024.1483182
22-25/05/2024, 6TH INTL CONF TRANSLATIONAL IMMUNOLOGY,
Poster: "Treg/CD137+ T cell balance as a novel biomarker of survival in advanced non-addicted NSCLC patients treated with immunotherapy as first-line” Tuosto L, Asquino A, Gelibter A, Strigari L, Zizzari IG, Scirocchi F, Pace A, Valentino F, Siringo M, Tramontano E, Botticelli A, Santini D, Nuti M, Rughetti A, Napoletano C
19-20/09/2024, SIPMeT 2024 - Translational Pathophysiology, Udine, Poster: “Novel circulating biomarkers in HNSCC patients receiving anti-PD1 therapy: the predictive and prognostic role of immunosuppressive CD137+Treg cells and LOX-1+PMN-MDSCs” Asquino A, Cirillo A, Strigari L, Pace A, Napoletano C, Tuosto L, Valentino F, Santini D, Nuti M, Botticelli A, Rughetti A, Zizzari IG
-2-4/12/2024, 36th AICC INTERNATIONAL MEETING- HIJACKING THE «GOOD» PATHWAYS: cancer, immunity and therapeutic approaches, Rome
Poster: “Soluble immune biomarkers to predict clinical outcome in head and neck patients treated with immunotherapy” Asquino A, Cirillo A, Strigari L, Pace A, Napoletano C, Tuosto L, Valentino F, Santini D, Nuti M, Botticelli A, Rughetti A, Zizzari IG
Poster: "C-type lectins in glioblastoma: immunosuppression players and potential
immune biomarkers" Pace A, Alfano C, Asquino A, Tuosto L, Valentino F, D'Angelo L, Napoletano C, Zizzari IG, Santoro A, Nuti M, Farina L, Petti M, Rughetti A