Phd in NETWORK ONCOLOGY AND PRECISION MEDICINE

Research: Investigating immune mechanisms and immune predictors of response to cancer therapies by network approach.

Education and training
FEBRUARY 2025 - JULY 2025: CIVIS course ‘‘Immuno-oncology in the spatial omics era’’, Sapienza University of Rome - University of Aix Marseille. 1-week of physical mobility to University of Aix Marseille.
FEBRUARY 2024 - JULY 2024: CIVIS course ‘‘Experimental models in Molecular Biomedicine’’, Sapienza University of Rome - Universidad Autonoma de Madrid (UAM). 1-week of physical mobility to UAM.
27/07/2023: Master's Degree in Medical Biotechnology (LM-9), University of Rome, La Sapienza. Experimental Thesis Title: ''Macrophage Galactose-type C lectin (MGL) and the carbohydrate ligand Tn as immunosuppression mediators in Glioblastoma patients''.
May 2023 - June 2023: CIVIS programme in Technical Innovation: Application to Immuno-Oncology, Sapienza University Of Rome, Italy. 1-week internship at the Head of Antibody Therapeutics and Immunotargeting (ATI) Team of Patrick Chames, CRCM, University of Aix Marseille.
20/10/2020: Bachelor's Degree in Biotechnology (L-2), University of Rome, La Sapienza. Thesis Title: ''Pymol as a CADD Platform''.
2017: High School Diploma, Isaac Newton Scientific High School, Rome.
Work experience
Position: 1/11/2023 - ON GOING PhD student in Network Oncology and Precision Medicine, Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza.
JANUARY 2026 - ON GOING: PhD mobility as International student at International school, Faculty of Medicine of KU Leuven, Leuven, Belgium.
Internship
Master's degree in Medical Biotechnology: Laboratory of Immunology of Tumors and Cell Therapies (Prof. Marianna Nuti), Department of Experimental Medicine, University of Rome La Sapienza.
Specific skills
Cell cultures (primary cultures and cell lines).
Western Blotting.
Isolation of PBMC from human blood of cancer patients by gradient centrifugation (Ficoll-Paque).
Freezing and thawing.
Immunophenotyping of myeloid suppressor cells (MDSC), T Reg, T exhausted, T cells by flow cytometry (Dx Flex, FACS Canto).
Data analysis with FlowJo and Prism.
Cell lysis and protein extraction.
Processing of tumor tissue of Glioblastoma (GB).
Microvescicles isolation.
ELISA essay.
MTT essay.
Senescence assay with β-Galactosidase.
In vitro treatment of cancer cell lines with targeted therapies.

Seminars
·       16/11/2023: FlowJo Course “Beyond conventional data analysis”.
·       13/12/2023: “The MyD88-adaptor-like (MAL) functions in chronic inflammatory pathways”, Prof. Mirza Baig.
·       2023: Soft skills 2023 edition.
·       12/02/2024: “Single cell transcriptomics”, Prof. Nikolaus Rajewsky, MDC Berlin.
·       13/02/2024: “Spatial omics and cancer”, Prof. Nikolaus Rajewsky, MDC Berlin.
·       15/02/2024: “Human brain organoids”, Prof. Nikolaus Rajewsky, MDC Berlin.
·       21/02/2024: “The role of atypical chemokine receptors in inflammatory responses”, Prof. Mattia LaLranchi.
·       03/04/2024: “Overcoming the obstacles faced by neoantigen cancer vaccines”, Prof. Peter D. Katsikis.
·       09/04/2024: “Novel nanotechnologies for biomedical applications”, Prof. Thanh Nguyen, UCL, London, UK.
·       31/10/2024: “Spatial transcriptomics applied to tumorigenesis and expsosomes”, Prof. Nikolaus Rajewsky.
·       04/10/2024: Meeting with Head of EU Medical Affairs, Incyte, Dr. Luca Marini.
·       08/10/2024: “Building a better induced regulatory T cell: Pitfalls, Progress, and Potential”, Prof. Lisa M. Minter.
·       14/10/2024: “Towards Non-coding RNA Therapeutics”, Prof. Frank Slack.
·       04–05/02/2025: “FISV PER I GIOVANI RICERCATORI. Advanced Technologies in Single Cell Omics”.
·       18/02/2025: “Molecular and functional characterization of circulating tumor cells”, Prof. Paola Gazzaniga.
·       07/03/2025: “Circadian clock disruption impairs immune oscillation in chronic endogenous hypercortisolism”, Valeria Hasemajer, RTDA.
·       02/04/2025: “New potential therapeutic targets for the reduction of chemotherapy-induced cardiotoxicity”, Prof. Sebastiano Sciarretta.
·       07/05/2025: “Tumorigenesis in space and time”, Prof. Nikolaus Rajewsky; Dr. Tancredi Massimo Pentimalli.
·       15/05/2025: “Spatial Transcriptomics, Pathology, and some news about Microplastics”, Prof. Nikolaus Rajewsky; Dr. Tancredi Massimo Pentimalli.
·       22/05/2025: “Micro- and circular RNAs”, Prof. Nikolaus Rajewsky.
·       12/06/2025: “Il microambiente tumorale della leucemia acuta a cellule T: nuove opportunità e limiti per l'immunoterapia”, Prof. Antonello Campese.
·       18/06/2025: “Biopsia liquida e cellule circolanti: ruolo in oncologia”, Prof. Paola Gazzaniga.