FEDERICA MAGGI

PhD Graduate

PhD program:: XXXIV

Thesis title: Effects of TRPV receptor agonists in Chronic Myeloid Leukemia

Chronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by accumulation of immature cells in bone marrow and peripheral blood. Although successful results were obtained with tyrosine kinase inhibitors, several patients show resistance. For this reason, the identification of new strategies and therapeutic biomarkers represent an attractive goal. The role of Transient Receptor Potential (TRP) ion channels as possible drug targets has been elucidated in different types of cancer. Among natural compounds known to activate TRPs, cannabidiol (CBD) and Capsaicin (CPS) display anti-cancer properties. By using FACS analysis, confocal microscopy, gene silencing and cell growth assay, we demonstrated that CBD, through TRPV2, inhibits cell proliferation and cell cycle in CML cells. It promoted mitochondria dysfunction and mitophagy as shown by mitochondrial mass reduction and up-regulation of several mitophagy markers. These effects were associated with changes in the expression of OCT-4 and PU.1 markers involved in stemness, and reduction of CD34 stemness marker. Moreover CBD, through the activation of autophagy, modulates the expression of CD274 gene in CML cells. Interestingly, a synergistic effect by combining CBD with the standard drug imatinib was found and imatinib-resistant cells remain susceptible to CBD effects. In addition, we demonstrated that CML cells are resistant to CPS, but not to N-oleoyldopamine (OLDA), another TRPV1 bioactive agonist whose biological functions are not yet fully explored, with anti-inflammatory activities. Our preliminary results show that OLDA induce CML cell death through reactive oxygen species (ROS) production, DNA damages and endoplasmatic reticulum (ER) stress activation. In conclusion, the targeting of TRPV2 by using CBD, and the targeting TRPV1 by using OLDA could be a promising strategy to enhance conventional therapy and improve the prognosis of CML patients.

Research products

11573/1617363 - 2022 - The effects of cannabidiol via TRPV2 channel in chronic myeloid leukemia cells and its combination with imatinib

Maggi, F.; Morelli, M. B.; Tomassoni, D.; Marinelli, O.; Aguzzi, C.; Zeppa, L.; Nabissi, M.; Santoni, G.; Amantini, C. - 01a Articolo in rivista

paper: CANCER SCIENCE (Oxford University Press:Journals Department, Great Clarendon Street, Oxford OX2 6DP United Kingdom:011 44 1865 556767, EMAIL: jnlorders@oup.co.uk, INTERNET: http://www.oup.co.uk, Fax: 011 44 1865 267485) pp. - - issn: 1347-9032 - wos: WOS:000764946300001 (15) - scopus: 2-s2.0-85125589141 (17)

11573/1617336 - 2022 - The Mucolipin TRPML2 Channel Enhances the Sensitivity of Multiple Myeloma Cell Lines to Ibrutinib and/or Bortezomib Treatment

Santoni, G.; Amantini, C.; Maggi, F.; Marinelli, O.; Santoni, M.; Morelli, M. B. - 01a Articolo in rivista

paper: BIOMOLECULES (Basel: MDPI) pp. 107- - issn: 2218-273X - wos: WOS:000748210100001 (5) - scopus: 2-s2.0-85122383083 (5)

11573/1617355 - 2022 - Functional In Vitro Assessment of VEGFA/NOTCH2 Signaling Pathway and pRB Proteasomal Degradation and the Clinical Relevance of Mucolipin TRPML2 Overexpression in Glioblastoma Patients

Santoni, G.; Amantini, C.; Nabissi, M.; Arcella, A.; Maggi, F.; Santoni, M.; Morelli, M. B. - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel (Matthaeustrasse 11) : Molecular Diversity Preservation International MDPI) pp. 688- - issn: 1661-6596 - wos: WOS:000746278100001 (4) - scopus: 2-s2.0-85122290926 (4)

11573/1617381 - 2022 - A3 Adenosine Receptor Antagonists with Nucleoside Structures and Their Anticancer Activity

Spinaci, A.; Buccioni, M.; Dal Ben, D.; Maggi, F.; Marucci, G.; Francucci, B.; Santoni, G.; Lambertucci, C.; Volpini, R. - 01a Articolo in rivista

paper: PHARMACEUTICALS (Basel : MDPI, Molecular Diversity Preservation International, 2004-) pp. 164- - issn: 1424-8247 - wos: WOS:000825149200001 (2) - scopus: 2-s2.0-85124157674 (3)

11573/1617312 - 2021 - Formulation and safety tests of a Wickerhamomyces anomalus-based product: potential use of killer toxins of a mosquito symbiotic yeast to limit malaria transmission

Cappelli, Alessia; Amantini, Consuelo; Maggi, Federica; Favia, Guido; Ricci, Irene - 01a Articolo in rivista

paper: TOXINS (Basel : Molecular Diversity Preservation International MDPI) pp. - - issn: 2072-6651 - wos: WOS:000711715100001 (4) - scopus: 2-s2.0-85117214381 (4)

11573/1496958 - 2021 - Tart cherry (Prunus cerasus L.) dietary supplement modulates visceral adipose tissue CB1 mRNA levels along with other adipogenesis-related genes in rat models of diet-induced obesity

Cocci, P.; Moruzzi, M.; Martinelli, I.; Maggi, F.; Micioni Di Bonaventura, M. V.; Cifani, C.; Mosconi, G.; Tayebati, S. K.; Damiano, S.; Lupidi, G.; Amantini, C.; Tomassoni, D.; Palermo, F. A. - 01a Articolo in rivista

paper: EUROPEAN JOURNAL OF NUTRITION (Heidelberg: Springer Darmstadt Germany:: Dr Dietrich Steinkopff Verlag) pp. - - issn: 1436-6207 - wos: WOS:000604159000005 (8) - scopus: 2-s2.0-85098485141 (8)

11573/1550046 - 2021 - Transient receptor potential (TRP) channels in haematological malignancies: an update

Maggi, Federica; Morelli, Maria Beatrice; Nabissi, Massimo; Marinelli, Oliviero; Zeppa, Laura; Aguzzi, Cristina; Santoni, Giorgio; Amantini, Consuelo - 01g Articolo di rassegna (Review)

paper: BIOMOLECULES (Basel: MDPI) pp. - - issn: 2218-273X - wos: WOS:000653432000001 (8) - scopus: 2-s2.0-85106145870 (8)

11573/1617331 - 2021 - Correlation between high pd-l1 and emt/invasive genes expression and reduced recurrence-free survival in blood-circulating tumor cells from patients with non-muscle-invasive bladder cancer

Morelli, M. B.; Amantini, C.; De Vermandois, J. A. R.; Gubbiotti, M.; Giannantoni, A.; Mearini, E.; Maggi, F.; Nabissi, M.; Marinelli, O.; Santoni, M.; Cimadamore, A.; Montironi, R.; Santoni, G. - 01a Articolo in rivista

paper: CANCERS (Basel: MDPI) pp. - - issn: 2072-6694 - wos: WOS:000734781900001 (9) - scopus: 2-s2.0-85119920765 (9)

11573/1617320 - 2021 - Ion channels alterations in the forebrain of high-fat diet fed rats

Roy, P.; Martinelli, I.; Moruzzi, M.; Maggi, F.; Amantini, C.; Micioni Di Bonaventura, M. V.; Cifani, C.; Amenta, F.; Tayebati, S. K.; Tomassoni, D. - 01a Articolo in rivista

paper: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Pavia : PagePress Publications, 2010) pp. - - issn: 2038-8306 - wos: WOS:000750052500017 (9) - scopus: 2-s2.0-85122167149 (9)

11573/1550044 - 2021 - Knock-down of mucolipin 1 channel promotes tumor progression and invasion in human glioblastoma cell lines

Santoni, G.; Amantini, C.; Nabissi, M.; Maggi, F.; Arcella, A.; Marinelli, O.; Eleuteri, A. M.; Santoni, M.; Morelli, M. B. - 01a Articolo in rivista

paper: FRONTIERS IN ONCOLOGY (Lausanne : Frontiers Editorial Office, 2011-) pp. - - issn: 2234-943X - wos: WOS:000646314800001 (9) - scopus: 2-s2.0-85105242332 (9)

11573/1617297 - 2021 - Mechanosensation and mechanotransduction in Natural Killer cells

Santoni, G.; Amantini, C.; Santoni, M.; Maggi, F.; Morelli, M. B.; Santoni, A. - 01g Articolo di rassegna (Review)

paper: FRONTIERS IN IMMUNOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. - - issn: 1664-3224 - wos: WOS:000679384100001 (18) - scopus: 2-s2.0-85111595602 (18)

11573/1496964 - 2020 - Biological function of PD-L2 and correlation with overall survival in type II endometrial cancer

Marinelli, O.; Annibali, D.; Morelli, M. B.; Zeppa, L.; Tuyaerts, S.; Aguzzi, C.; Amantini, C.; Maggi, F.; Ferretti, B.; Santoni, G.; Amant, F.; Nabissi, M. - 01a Articolo in rivista

paper: FRONTIERS IN ONCOLOGY (Lausanne : Frontiers Editorial Office, 2011-) pp. - - issn: 2234-943X - wos: WOS:000588037700001 (9) - scopus: 2-s2.0-85095809947 (10)

11573/1496961 - 2020 - The effects of cannabidiol and prognostic role of TRPV2 in human endometrial cancer

Marinelli, O.; Morelli, M. B.; Annibali, D.; Aguzzi, C.; Zeppa, L.; Tuyaerts, S.; Amantini, C.; Amant, F.; Ferretti, B.; Maggi, F.; Santoni, G.; Nabissi, M. - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel (Matthaeustrasse 11) : Molecular Diversity Preservation International MDPI) pp. - - issn: 1661-6596 - wos: WOS:000559012500001 (30) - scopus: 2-s2.0-85088938547 (31)

11573/1496947 - 2020 - Effects of prunus cerasus L. Seeds and juice on liver steatosis in an animal model of diet-induced obesity

Martinelli, Ilenia; Di Bonaventura, Maria Vittoria Micioni; Moruzzi, Michele; Amantini, Consuelo; Maggi, Federica; Gabrielli, Maria Gabriella; Fruganti, Alessandro; Marchegiani, Andrea; Dini, Fabrizio; Marini, Carlotta; Polidori, Carlo; Lupidi, Giulio; Amenta, Francesco; Tayebati, Seyed Khosrow; Cifani, Carlo; Tomassoni, Daniele - 01a Articolo in rivista

paper: NUTRIENTS (Basel, Switzerland: Molecular Diversity Preservation International (MDPI)) pp. - - issn: 2072-6643 - wos: WOS:000542272700219 (13) - scopus: 2-s2.0-85084392966 (16)

11573/1417398 - 2020 - Pathophysiological role of transient receptor potential mucolipin channel 1 in calcium-mediated stress-induced neurodegenerative diseases

Santoni, G.; Maggi, F.; Amantini, C.; Marinelli, O.; Nabissi, M.; Morelli, M. B. - 01g Articolo di rassegna (Review)

paper: FRONTIERS IN PHYSIOLOGY (Lausanne: Frontiers Research Foundation, 2010-) pp. - - issn: 1664-042X - wos: WOS:000597676500001 (19) - scopus: 2-s2.0-85083110549 (20)

11573/1496954 - 2020 - Cross-talk between microRNAs, long non-coding RNAs and p21Cip1 in glioma: diagnostic, prognostic and therapeutic roles

Santoni, Giorgio; Morelli, Maria Beatrice; Nabissi, Massimo; Maggi, Federica; Marinelli, Oliviero; Santoni, Matteo; Amantini, Consuelo - 01g Articolo di rassegna (Review)

paper: JOURNAL OF CANCER METASTASIS AND TREATMENT () pp. - - issn: 2394-4722 - wos: WOS:000911185200022 (2) - scopus: 2-s2.0-85111281940 (3)

11573/1330315 - 2019 - Expression profiling of circulating tumor cells in pancreatic ductal adenocarcinoma patients: biomarkers predicting overall survival

Amantini, C.; Morelli, M. B.; Nabissi, M.; Piva, F.; Marinelli, O.; Maggi, F.; Bianchi, F.; Bittoni, A.; Berardi, R.; Giampieri, R.; Santoni, G. - 01a Articolo in rivista

paper: FRONTIERS IN ONCOLOGY (Lausanne : Frontiers Editorial Office, 2011-) pp. - - issn: 2234-943X - wos: WOS:000484972500001 (53) - scopus: 2-s2.0-85072998841 (52)

11573/1330300 - 2019 - Typical and atypical circulating tumour cells in bladder cancer. Why improve our knowledge?

Maggi, Federica; Amantini, Consuelo; Nabissi, Massimo; Oliviero, Marinelli; Santoni, Giorgio; Morelli, Maria Beatrice - 01a Articolo in rivista

paper: CLINICS ONCOLOGY (Middletown DE: Clinics Oncology) pp. - - issn: 2688-562X - wos: (0) - scopus: (0)

11573/1330306 - 2019 - The controversial role of PD-1 and its ligands in gynecological malignancies

Marinelli, O.; Annibali, D.; Aguzzi, C.; Tuyaerts, S.; Amant, F.; Morelli, M. B.; Santoni, G.; Amantini, Consuelo; Maggi, Federica; Nabissi, M. - 01g Articolo di rassegna (Review)

paper: FRONTIERS IN ONCOLOGY (Lausanne : Frontiers Editorial Office, 2011-) pp. - - issn: 2234-943X - wos: WOS:000497837400001 (29) - scopus: 2-s2.0-85074285501 (29)

11573/1348291 - 2019 - Effects of Prunus cerasus L. seeds and juice on liver steatosis in an animal model of diet-induced obesity.

Martinelli, I; Moruzzi, M; Maggi, F; Amantini, C; Palermo, F A; Cocci, P; Micioni Di Bonaventura, M V; Gabrielli, G; Fruganti, A; Marchegiani, A; Kloeting, N; Lupidi, G; Cifani, C; Seyed, K T; Tomassoni, D - 04f Poster

conference: Cibo e nutraceutici: parola chiave "CARATTERIZZAZIONE" (Camerino, 62032 (MC))

book: Cibo e nutraceutici: parola chiave "CARATTERIZZAZIONE" - (9788867680405)

11573/1348277 - 2019 - The TRPV2 cation channels: from urothelial cancer invasiveness to glioblastoma multiforme interactome signature

Santoni, G.; Amantini, C.; Maggi, F.; Marinelli, O.; Santoni, M.; Nabissi, M.; Morelli, M. B. - 01g Articolo di rassegna (Review)

paper: LABORATORY INVESTIGATION (Lippincott, Williams & Wilkins:530 Walnut Street:Philadelphia, PA 19106:(800)638-3030, (301)223-2300, EMAIL: orders@lww.com, INTERNET: http://www.lww.com, Fax: (301)223-2320, (301)223-2320) pp. - - issn: 0023-6837 - wos: WOS:000510902400002 (30) - scopus: 2-s2.0-85074570889 (32)

11573/1348283 - 2019 - Transient receptor potential cation channels in cancer therapy

Santoni, Giorgio; Maggi, Federica; Morelli, Maria Beatrice; Santoni, Matteo; Marinelli, Oliviero - 01g Articolo di rassegna (Review)

paper: MEDICAL SCIENCES (Basel : Multidisciplinary Digital Publishing Institute) pp. - - issn: 2076-3271 - wos: (0) - scopus: 2-s2.0-85149157403 (33)