SARA ELSA AITA

Dottoressa di ricerca

ciclo: XXXVI


supervisore: Prof.ssa Anna Laura Capriotti

Titolo della tesi: CHARACTERIZATION OF BIOACTIVE COMPOUNDS IN AGRIFOOD PRODUCTS

In the last two decades, agri-food matrices have assumed a fundamental role as a source of sustenance and a reservoir of beneficial chemical compounds. For this purpose, research increasingly aims to make food the focal point of our health in preventing and improving immune responses. The identification of bioactive compounds and the subsequent enhancement of agri- food products have thus become the forefront of research. Functional foods have taken on an increasingly important role in recent years, thanks to their ability to maintain good health by preventing various pathologies due to their content of bioactive molecules. When appropriately isolated, characterized, and quantitatively determined, these substances can be used in the formulation of so-called nutraceutical compounds. The importance of characterizing bioactive compounds is not only linked to discovering molecules with beneficial effects on health. Still, it can also help detect possible food adulterations or determine composition and genuineness. To comprehensively characterize food and isolate individual bioactive compounds or phyto- complexes, it is necessary to use and develop advanced analytical techniques for extraction, purification, separation, and characterization. Liquid chromatography coupled with high-resolution mass spectrometry is currently the most effective tool for obtaining a "fingerprint" of the examined food matrix. In this doctoral project, ample attention will be given to some classes of bioactive molecules with a specific focus on the polar lipidome of hemp. Although phytocannabinoids are the most recognized hemp bioactives due to their therapeutic potential, other lesser-studied classes of chemicals can exert synergistic effects to enhance the bioactivities of phytocannabinoids and other health-promoting activities. As such, a detailed characterization of the composition of this plant could help future research further exploit the beneficial effects of hemp compounds on human health. In this context, this doctoral project contributes to a detailed characterization of minor components of industrial hemp in terms of fatty acids, policosanols, carotenoids, and fat-soluble vitamins. The analytical approaches commonly used for determining fatty acids and policosanols involve gas chromatographic techniques with prior derivatization of the compounds under examination. The development of more straightforward methods based on liquid chromatography techniques, which do not require chemical derivatization, is one of the goals that this doctoral thesis achieved. The localization of double bonds is a crucial step for the structural characterization of fatty acids. For this reason, another aim of this thesis was the development of a procedure that allows the identification of carbon-carbon double bond locations. In particular, although the consumption of hempseeds and its derivatives is rapidly increasing for the high nutritional value of its bioactive compounds, including a rich lipidome with elevated concentrations in PUFAs with optimum ω- 6/ω-3 ratio, comprehensive characterization of the polar lipidome was missing, especially regarding the regioisomer composition of fatty acids bound to glycerophospholipids. To achieve this goal, an analytical platform, including derivatization by a photochemical [2+2] cycloaddition with 6- azauracil was developed for pinpointing the regiochemistry of carbon-carbon double bonds in polar lipids extracted from hempseeds. This approach allowed the determination of double bond locations of free and conjugated fatty acids, that is essential to further understand their biological roles and biophysical properties. In the wide range of studied phytochemical compounds in industrial hemp, carotenoids and fat- soluble vitamins have scarcely been considered, even though their various bioactivities. For the first time, in this doctoral project, the carotenoid and fat-soluble vitamin profile of Cannabis inflorescences has been extensively characterized in both qualitative and quantitative fashion after an extraction approach aimed at avoiding the generation of artifacts. Another aim of this thesis was the development of proper mass-spectrometric and data-analysis methods for the comprehensive characterization of phenolic compounds. Thanks to their demonstrated potential health benefits, phenolic compounds are perhaps the most explored natural compounds. Hence, exploring and identifying new phenolic compounds from natural sources have become a primary research focus in developing natural products. In particular, since Andean berries are a rich source of a wide variety of phenolic compounds, untargeted MS analyses coupled with a dedicated data processing workflow allowed us to expand the current knowledge on these berries grown in the unique Andean ecosystem, also improving our understanding of the fate of phenolic compounds after fermentation. Moreover, an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papain-like protease (PLpro) activity was developed. Therefore, this doctoral project aimed to develop advanced analytical methodologies for determining these classes of bioactive compounds and overcoming obstacles still present in their extraction, identification, and complete characterization.

Produzione scientifica

11573/1709854 - 2024 - An untargeted analytical workflow based on Kendrick mass defect filtering reveals dysregulations in acylcarnitines in prostate cancer tissue
Cerrato, Andrea; Aita, Sara Elsa; Biancolillo, Alessandra; Laganà, Aldo; Marini, Federico; Montone, Carmela Maria; Rosati, Davide; Salciccia, Stefano; Sciarra, Alessandro; Taglioni, Enrico; Capriotti, Anna Laura - 01a Articolo in rivista
rivista: ANALYTICA CHIMICA ACTA (New York; Amsterdam: Elsevier) pp. - - issn: 0003-2670 - wos: WOS:001218400700001 (0) - scopus: 2-s2.0-85190977333 (0)

11573/1705749 - 2024 - One-phase extraction coupled with photochemical reaction allows the in-depth lipid characterization of hempseeds by untargeted lipidomics
Cerrato, Andrea; Aita, Sara Elsa; Cannazza, Giuseppe; Cavaliere, Chiara; Cavazzini, Alberto; Citti, Cinzia; Montone, Carmela Maria; Taglioni, Enrico; Laganà, Aldo - 01a Articolo in rivista
rivista: TALANTA (-Oxford, United Kingdom: Elsevier Science Limited Oxford: Pergamon, 1958-) pp. - - issn: 0039-9140 - wos: WOS:001172418200001 (0) - scopus: 2-s2.0-85182729591 (1)

11573/1697092 - 2023 - Untargeted Analysis of Short-Chain Peptides in Urine Samples Short Peptides Analysis
Aita, Sara Elsa; Cerrato, Andrea; Laganà, Aldo; Montone, Carmela Maria; Taglioni, Enrico; Capriotti, Anna Laura - 02a Capitolo o Articolo
libro: 2745 - (978-1-0716-3576-6; 978-1-0716-3577-3)

11573/1669562 - 2023 - Evaluation of the carotenoid and fat-soluble vitamin profile of industrial hemp inflorescence by liquid chromatography coupled to mass spectrometry and photodiode-array detection
Cerrato, Andrea; Aita, Sara Elsa; Cannazza, Giuseppe; Capriotti, Anna Laura; Cavaliere, Chiara; Citti, Cinzia; Bosco, Chiara Dal; Gentili, Alessandra; Montone, Carmela Maria; Paris, Roberta; Laganà, Aldo - 01a Articolo in rivista
rivista: JOURNAL OF CHROMATOGRAPHY A ([S.l.] : Elsevier Science) pp. 1-9 - issn: 1873-3778 - wos: WOS:000930833100001 (4) - scopus: 2-s2.0-85147539003 (5)

11573/1669568 - 2023 - Untargeted analysis of environmental contaminants in surface snow samples of Svalbard Islands by liquid chromatography-high resolution mass spectrometry
Giannelli Moneta, Benedetta; Aita, Sara Elsa; Barbaro, Elena; Capriotti, Anna Laura; Cerrato, Andrea; Laganà, Aldo; Montone, Carmela Maria; Piovesana, Susy; Scoto, Federico; Barbante, Carlo; Cavaliere, Chiara - 01a Articolo in rivista
rivista: SCIENCE OF THE TOTAL ENVIRONMENT (Society for Enviornmental Geochemistry and Health) pp. 1-8 - issn: 1879-1026 - wos: WOS:000938721500007 (4) - scopus: 2-s2.0-85140608673 (5)

11573/1691743 - 2023 - Occurrence of per- and polyfluorinated alkyl substances in wastewater treatment plants in Northern Italy
Moneta, Benedetta Giannelli; Feo, Maria Luisa; Torre, Marco; Tratzi, Patrizio; Aita, Sara Elsa; Montone, Carmela Maria; Taglioni, Enrico; Mosca, Silvia; Balducci, Catia; Cerasa, Marina; Guerriero, Ettore; Petracchini, Francesco; Cavaliere, Chiara; Laganà, Aldo; Paolini, Valerio - 01a Articolo in rivista
rivista: SCIENCE OF THE TOTAL ENVIRONMENT (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598 Tokyo ; Oxford ; Lausanne ; New York ; Shannon ; Amsterdam : Elsevier) pp. 1-11 - issn: 0048-9697 - wos: WOS:001032956900001 (7) - scopus: 2-s2.0-85163773364 (8)

11573/1669564 - 2023 - Biotic transformation products of sulfonamides in environmental water samples. High-resolution mass spectrometry-based tentative identification by a suspect screening approach
Montone, Carmela Maria; Giannelli Moneta, Benedetta; Aita, Sara Elsa; Capriotti, Anna Laura; Cerrato, Andrea; Laganà, Aldo; Marchetti, Angela; Piovesana, Susy; Villano, Marianna; Cavaliere, Chiara - 01a Articolo in rivista
rivista: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (Oxford ; New York : Pergamon Press Amsterdam : Elsevier Science) pp. 1-9 - issn: 0731-7085 - wos: WOS:000939536700001 (6) - scopus: 2-s2.0-85147961208 (6)

11573/1614558 - 2022 - Detailed investigation of the composition and transformations of phenolic compounds in fresh and fermented Vaccinium floribundum berry extracts by high-resolution mass spectrometry and bioinformatics
Cerrato, A.; Piovesana, S.; Aita, S. E.; Cavaliere, C.; Felletti, S.; Lagana, A.; Montone, C. M.; Vargas-De-La-Cruz, C.; Capriotti, A. L. - 01a Articolo in rivista
rivista: PHYTOCHEMICAL ANALYSIS (John Wiley & Sons Limited:1 Oldlands Way, Bognor Regis, P022 9SA United Kingdom:011 44 1243 779777, EMAIL: cs-journals@wiley.co.uk, INTERNET: http://www.wiley.co.uk, Fax: 011 44 1243 843232) pp. 507-516 - issn: 0958-0344 - wos: WOS:000745318700001 (3) - scopus: 2-s2.0-85123488098 (5)

11573/1652465 - 2022 - Investigating the short peptidome profile of Italian dry-cured ham at different processing times by high-resolution mass spectrometry and chemometrics
Cerrato, Andrea; Aita, Sara Elsa; Capriotti, Anna Laura; Cavaliere, Chiara; Montone, Angela Michela Immacolata; Montone, Carmela Maria; Laganà, Aldo - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:000775275800001 (7) - scopus: 2-s2.0-85126591361 (8)

11573/1593477 - 2022 - Untargeted analysis of contaminants in river water samples: Comparison between two different sorbents for solid-phase extraction followed by liquid chromatography-high-resolution mass spectrometry determination
Montone, C. M.; Giannelli Moneta, B.; Aita, S. E.; Aulenta, F.; Cavaliere, C.; Cerrato, A.; Fazi, S.; Lagana, A.; Paolini, V.; Petracchini, F.; Piovesana, S.; Capriotti, A. L. - 01a Articolo in rivista
rivista: MICROCHEMICAL JOURNAL (Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445) pp. 1-8 - issn: 0026-265X - wos: WOS:000719818300001 (6) - scopus: 2-s2.0-85118851011 (6)

11573/1545589 - 2021 - Andean blueberry of the Genus Disterigma: a high-resolution mass spectrometric approach for the comprehensive characterization of phenolic compounds
Aita, Sara Elsa; Capriotti, Anna Laura; Cavaliere, Chiara; Cerrato, Andrea; Giannelli Moneta, Benedetta; Montone, Carmela Maria; Piovesana, Susy; Laganà, Aldo - 01a Articolo in rivista
rivista: SEPARATIONS (Basel : MDPI AG, 2016-) pp. - - issn: 2297-8739 - wos: WOS:000654464500001 (19) - scopus: 2-s2.0-85106165550 (20)

11573/1472162 - 2021 - A rapid and innovative extraction and enrichment method for the metaproteomic characterization of dissolved organic matter in groundwater samples
Capriotti, A. L.; Aita, S. E.; Cavaliere, C.; Cerrato, A.; Montone, C. M.; Piovesana, S.; Lagana, A. - 01a Articolo in rivista
rivista: JOURNAL OF SEPARATION SCIENCE (Wiley-VCH Verlag GmBH:PO Box 101161, D 69451 Weinheim Germany:011 49 6201 606147, EMAIL: service@wiley-vch.de, INTERNET: http://www.wiley-vch.de, Fax: 011 49 6201 606328) pp. 1612-1620 - issn: 1615-9306 - wos: WOS:000597799700001 (0) - scopus: 2-s2.0-85097434901 (0)

11573/1541232 - 2021 - Degradation of the polar lipid and fatty acid molecular species in extra virgin olive oil during storage based on shotgun lipidomics
Capriotti, Anna Laura; Cerrato, Andrea; Aita, Sara Elsa; Montone, Carmela Maria; Piovesana, Susy; Laganà, Aldo; Cavaliere, Chiara - 01a Articolo in rivista
rivista: JOURNAL OF CHROMATOGRAPHY A (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. 1-9 - issn: 0021-9673 - wos: WOS:000687137800005 (11) - scopus: 2-s2.0-85100205199 (12)

11573/1549463 - 2021 - Production and characterization of medium‐sized and short antioxidant peptides from soy flour‐simulated gastrointestinal hydrolysate
Cavaliere, Chiara; Montone, Angela Michela Immacolata; Aita, Sara Elsa; Capparelli, Rosanna; Cerrato, Andrea; Cuomo, Paola; Lagana, Aldo; Montone, Carmela Maria; Piovesana, Susy; Capriotti, Anna Laura - 01a Articolo in rivista
rivista: ANTIOXIDANTS (Basel: MDPI) pp. - - issn: 2076-3921 - wos: WOS:000653353700001 (14) - scopus: 2-s2.0-85105136805 (16)

11573/1592042 - 2021 - Fully Automatized Detection of Phosphocholine-Containing Lipids through an Isotopically Labeled Buffer Modification Workflow
Cerrato, A.; Aita, S. E.; Capriotti, A. L.; Cavaliere, C.; Montone, C. M.; Piovesana, S.; Lagana, A. - 01a Articolo in rivista
rivista: ANALYTICAL CHEMISTRY (Washington, DC : American Chemical Society, 1948-) pp. 15042-15048 - issn: 1520-6882 - wos: WOS:000721110000018 (5) - scopus: 2-s2.0-85119267366 (5)

11573/1675341 - 2021 - Methodologies for extraction and separation of short-chain bioactive peptides
Cerrato, A.; Aita, S. E.; Montone, C. M.; Capriotti, A. L.; Piovesana, S.; Laganà, A. - 02a Capitolo o Articolo
libro: Biologically Active Peptides From Basic Science to Applications for Human Health - (978-0-12-821389-6)

11573/1675340 - 2021 - Analytical methodologies for lipidomics in hemp plant
Cerrato, A.; Capriotti, A. L.; Montone, C. M.; Aita, S. E.; Cannazza, G.; Citti, C.; Piovesana, S.; Aldo, L. - 02a Capitolo o Articolo
libro: Methods in Molecular Biology - (978-1-0716-1409-9; 978-1-0716-1410-5)

11573/1472170 - 2021 - Comprehensive identification of native medium-sized and short bioactive peptides in sea bass muscle
Cerrato, Andrea; Aita, Sara Elsa; Cavaliere, Chiara; Laganà, Aldo; Montone, Carmela Maria; Piovesana, Susy; Zenezini Chiozzi, Riccardo; Capriotti, Anna Laura - 01a Articolo in rivista
rivista: FOOD CHEMISTRY (Elsevier Applied Science:An Imprint of Elsevier Science Ltd, The Boulevard, Langford Lane, Kidlington Oxford OX5 1GB United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: nlinfo-f@elsevier.nl OR usinfo-f@elsevier.com OR forinfo-kyf04035@niftyserve.or.jp, INTERNET: http://www.elsevier.nl/, Fax: 011 44 1865 843010) pp. - - issn: 0308-8146 - wos: WOS:000605479300002 (22) - scopus: 2-s2.0-85094806393 (28)

11573/1592040 - 2021 - Characterization of the trans-epithelial transport of green tea (C. sinensis) catechin extracts with in vitro inhibitory effect against the sars-cov-2 papain-like protease activity
Montone, C. M.; Aita, S. E.; Arnoldi, A.; Capriotti, A. L.; Cavaliere, C.; Cerrato, A.; Lammi, C.; Piovesana, S.; Ranaldi, G.; Lagana, A. - 01a Articolo in rivista
rivista: MOLECULES (Basel: MDPI Berlin: Springer, 1996-) pp. - - issn: 1420-3049 - wos: WOS:000721073900001 (7) - scopus: 2-s2.0-85119624437 (11)

11573/1568942 - 2021 - Profiling and quantitative analysis of underivatized fatty acids in Chlorella vulgaris microalgae by liquid chromatography-high resolution mass spectrometry
Montone, C. M.; Aita, S. E.; Catani, M.; Cavaliere, C.; Cerrato, A.; Piovesana, S.; Lagana, A.; Capriotti, A. L. - 01a Articolo in rivista
rivista: JOURNAL OF SEPARATION SCIENCE (Wiley-VCH Verlag GmBH:PO Box 101161, D 69451 Weinheim Germany:011 49 6201 606147, EMAIL: service@wiley-vch.de, INTERNET: http://www.wiley-vch.de, Fax: 011 49 6201 606328) pp. 3041-3051 - issn: 1615-9306 - wos: WOS:000665610500001 (3) - scopus: 2-s2.0-85108425078 (6)

11573/1592038 - 2021 - High-resolution mass spectrometry and chemometrics for the detailed characterization of short endogenous peptides in milk by-products
Montone, C. M.; Aita, S. E.; Cavaliere, C.; Cerrato, A.; Lagana, A.; Piovesana, S.; Capriotti, A. L. - 01a Articolo in rivista
rivista: MOLECULES (Basel: MDPI Berlin: Springer, 1996-) pp. 1-14 - issn: 1420-3049 - wos: WOS:000718414800001 (4) - scopus: 2-s2.0-85118414370 (5)

11573/1568939 - 2021 - Targeted and untargeted characterization of underivatized policosanols in hemp inflorescence by liquid chromatography-high resolution mass spectrometry
Montone, Carmela Maria.; Aita, Sara Elsa; Cannazza, Giuseppe; Cavaliere, Chiara; Cerrato, Andrea; Citti, Cinzia; Mondello, Luigi; Piovesana, Susy; Laganà, Aldo; Capriotti, Anna Laura - 01a Articolo in rivista
rivista: TALANTA (-Oxford, United Kingdom: Elsevier Science Limited Oxford: Pergamon, 1958-) pp. - - issn: 0039-9140 - wos: WOS:000700078500004 (4) - scopus: 2-s2.0-85111833804 (4)

11573/1540944 - 2021 - In-depth cannabis fatty acid profiling by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry
Piovesana, S.; Aita, S. E.; Cannazza, G.; Capriotti, A. L.; Cavaliere, C.; Cerrato, A.; Guarnaccia, P.; Montone, C. M.; Lagana, A. - 01a Articolo in rivista
rivista: TALANTA (-Oxford, United Kingdom: Elsevier Science Limited Oxford: Pergamon, 1958-) pp. 1-7 - issn: 0039-9140 - wos: WOS:000657101200040 (6) - scopus: 2-s2.0-85101859928 (8)

11573/1423131 - 2020 - A new opening for the tricky untargeted investigation of natural and modified short peptides
Cerrato, A.; Aita, S. E.; Capriotti, A. L.; Cavaliere, C.; Montone, C. M.; Lagana, A.; Piovesana, S. - 01a Articolo in rivista
rivista: TALANTA (-Oxford, United Kingdom: Elsevier Science Limited Oxford: Pergamon, 1958-) pp. 1-6 - issn: 0039-9140 - wos: WOS:000568747200009 (28) - scopus: 2-s2.0-85086630266 (31)

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