VALERIA PENNACCHIETTI

PhD Student

PhD program:: XXXVII
email: valeria.pennacchietti@uniroma1.it
phone: 0649910879
building: CU027 - Dipartimento di Scienze Biochimiche "A. Rossi Fanelli"
room: 217

supervisor: Prof. Stefano Gianni
advisor: Prof.ssa Francesca Cutruzzolà
co-supervisor: Prof.ssa Francesca Cutruzzolà

Research: Understanding the folding and binding mechanism of protein-protein recognition domains

Thematic area: Biophysics, Biochemistry, Molecular biology
SSD:BIO/10; BIO/11

Education:

November 2021
PhD in Life Sciences, XXXVII cycle, at the “La Sapienza” University of Rome, Department of Biochemical Sciences “A. Rossi Fanelli”.

October 2019 – July 2021
Master's Degree in Pharmaceutical Biotechnology (LM-9) at the “La Sapienza” University of Rome, with a score of 110/110 cum laude

October 2016 – October 2019
Bachelor's degree in Applied Pharmaceutical Sciences (L-29) at the “La Sapienza” University of Rome, with a score of 110/110 cum laude

PhD project:

Adapter proteins are a class of proteins that play a fundamental role in the context of regulating cellular homeostasis, acting as a dock for different signaling molecules, allowing them to form supramolecular complexes and become functional. In particular, the FRS2 protein (fibroblast growth factor receptor substrate 2), through its PTB domain, plays a crucial role in signaling pathways. Especially those involving the fibroblast growth factor and nerve growth factor receptor families. Overexpression of the FRS2 protein can lead to the formation of different types of tumors. Through the use of molecular biology, biochemistry and biophysics methodologies it was possible to characterize the folding mechanism of the PTB domain and the interaction between it and two ligands, FGFR1 and TRKB. It has been observed that the presence or absence of a disulfide bridge probably determines a change in the conformation of the protein. Furthermore, it has been observed that this variation does not particularly influence the interaction with physiological ligands.

Participation in funded research projects:
- Projects "Avvio alla ricerca" (2023)
Responsabile: PENNACCHIETTI VALERIA
Title: "Understanding molecular and structural determinants of the recognition mechanisms of the H.pylori CagA protein"
-Projects "Avvio alla ricerca" (2022)
Responsabile: PENNACCHIETTI VALERIA
Title: "Analysing the NTAIL interaction with the PXD domain to develop antiviral strategies against Paramyxoviridae"

Courses:
-Bioinformatics: Theory and Applications From Genomes To Drugs
-24th Bologna Winter School: Bioinformatics and Deep learning for biodata analysis

Research products

11573/1706810 - 2024 - The binding selectivity of the C-terminal SH3 domain of Grb2, but not its folding pathway, is dictated by its contiguous SH2 domain

Di Felice, Mariana; Pagano, Livia; Pennacchietti, Valeria; Diop, Awa; Pietrangeli, Paola; Marcocci, Lucia; Di Matteo, Sara; Malagrinò, Francesca; Toto, Angelo; Gianni, Stefano - 01a Articolo in rivista

paper: THE JOURNAL OF BIOLOGICAL CHEMISTRY (American Society for Biochemistry and Molecular Biology:9650 Rockville Pike:Bethesda, MD 20814:(301)530-7145, EMAIL: asbmb@asbmb.faseb.org, INTERNET: http://www.faseb.org/asbmb, Fax: (301)571-1824) pp. - - issn: 0021-9258 - wos: (0) - scopus: 2-s2.0-85188713900 (2)

11573/1711132 - 2024 - PDZ2-conjugated-PLGA nanoparticles are tiny heroes in the battle against SARS-CoV-2

Giacon, Noah; Lo Cascio, Ettore; Pennacchietti, Valeria; De Maio, Flavio; Santarelli, Giulia; Sibilia, Diego; Tiberio, Federica; Sanguinetti, Maurizio; Lattanzi, Wanda; Toto, Angelo; Arcovito, Alessandro - 01a Articolo in rivista

paper: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. - - issn: 2045-2322 - wos: WOS:001244410300034 (5) - scopus: (0)

11573/1702558 - 2024 - Exploring the short linear motif-mediated protein-protein interactions of CrkL through ProP-PD

Pagano, L.; Simonetti, L.; Pennacchietti, V.; Toto, A.; Malagrinò, F.; Ivarsson, Y.; Gianni, S. - 01a Articolo in rivista

paper: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (San Diego [etc.]: Elsevier [etc.]) pp. 149658- - issn: 0006-291X - wos: WOS:001197686500001 (0) - scopus: 2-s2.0-85186076885 (0)

11573/1711822 - 2024 - Folding and binding kinetics of the tandem of SH2 domains from SHP2

Pagano, Livia; Pennacchietti, Valeria; Malagrinò, Francesca; Di Felice, Mariana; Toso, Julian; Puglisi, Elena; Gianni, Stefano; Toto, Angelo - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:001255961100001 (0) - scopus: (0)

11573/1726961 - 2024 - A PDZ tandem repeat folds and unfolds via different pathways

Pennacchietti, Valeria; Di Matteo, Sara; Pagano, Livia; Toplek, Fran Bačić; Stegani, Bruno; Toto, Angelo; Toso, Julian; Puglisi, Elena; Capelli, Riccardo; Di Felice, Mariana; Malagrinò, Francesca; Camilloni, Carlo; Gianni, Stefano - 01a Articolo in rivista

paper: PROTEIN SCIENCE (Cambridge University Press / New York:40 West 20th Street:New York, NY 10011:(800)872-7423, (212)924-3900, EMAIL: journals_subscriptions@cup.org, INTERNET: http://www.journals.cambridge.org, Fax: (212)691-3239) pp. - - issn: 0961-8368 - wos: (0) - scopus: (0)

11573/1707732 - 2024 - The mechanism of folding of human frataxin in comparison to the yeast homologue – broad energy barriers and the general properties of the transition state

Pietrangeli, Paola; Marcocci, Lucia; Pennacchietti, Valeria; Diop, Awa; Di Felice, Mariana; Pagano, Livia; Malagrinò, Francesca; Toto, Angelo; Brunori, Maurizio; Gianni, Stefano - 01a Articolo in rivista

paper: JOURNAL OF MOLECULAR BIOLOGY (-LONDON, ENGLAND: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, -London; New York: Academic Press.) pp. - - issn: 0022-2836 - wos: WOS:001223623100001 (0) - scopus: 2-s2.0-85189293931 (0)

11573/1683686 - 2023 - Biophysical characterization of the binding mechanism between the MATH domain of SPOP and its physiological partners

Diop, Awa; Pietrangeli, Paola; Nardella, Caterina; Pennacchietti, Valeria; Pagano, Livia; Toto, Angelo; Di Felice, Mariana; Di Matteo, Sara; Marcocci, Lucia; Malagrinò, Francesca; Gianni, Stefano - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 10138- - issn: 1422-0067 - wos: WOS:001017317000001 (2) - scopus: 2-s2.0-85163977385 (2)

11573/1695759 - 2023 - Addressing the binding mechanism of the Meprin and TRAF-C hmology domain of the Speckle-Type POZ protein using protein engineering

Diop, Awa; Pietrangeli, Paola; Pennacchietti, Valeria; Pagano, Livia; Toto, Angelo; Di Felice, Mariana; Di Matteo, Sara; Marcocci, Lucia; Malagrinò, Francesca; Gianni, Stefano - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:001131010700001 (0) - scopus: 2-s2.0-85180652606 (1)

11573/1682765 - 2023 - Monomeric and dimeric states of human ZO1-PDZ2 are functional partners of the SARS-CoV-2 E protein

Giacon, Noah; Lo Cascio, Ettore; Davidson, Darcy S.; Polêto, Marcelo D.; Lemkul, Justin A.; Pennacchietti, Valeria; Pagano, Livia; Zamparelli, Carlotta; Toto, Angelo; Arcovito, Alessandro - 01a Articolo in rivista

paper: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL (Göteborg : Chalmers University of Technology, 2011-) pp. 3259-3271 - issn: 2001-0370 - wos: WOS:001020735300001 (1) - scopus: 2-s2.0-85161275182 (1)

11573/1685968 - 2023 - An intramolecular energetic network regulates ligand recognition in a SH2 domain

Nardella, Caterina; Pagano, Livia; Pennacchietti, Valeria; Felice, Mariana Di; Matteo, Sara Di; Diop, Awa; Malagrinò, Francesca; Marcocci, Lucia; Pietrangeli, Paola; Gianni, Stefano; Toto, Angelo - 01a Articolo in rivista

11573/1686025 - 2023 - Characterization of the folding and binding properties of the PTB domain of FRS2 with phosphorylated and unphosphorylated ligands

Pennacchietti, Valeria; Pagano, Livia; Malagrinò, Francesca; Diop, Awa; Di Felice, Mariana; Di Matteo, Sara; Marcocci, Lucia; Pietrangeli, Paola; Toto, Angelo; Gianni, Stefano - 01a Articolo in rivista

paper: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (Attuale:ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, USA, NY, 10010-1710 Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445) pp. 109703- - issn: 0003-9861 - wos: WOS:001053305400001 (0) - scopus: 2-s2.0-85166633882 (0)

11573/1679810 - 2023 - Different electrostatic forces drive the binding kinetics of SARS-CoV, SARS-CoV-2 and MERS-CoV Envelope proteins with the PDZ2 domain of ZO1

Pennacchietti, Valeria; Toto, Angelo - 01a Articolo in rivista

paper: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. - - issn: 2045-2322 - wos: WOS:000992335400005 (2) - scopus: 2-s2.0-85159461892 (2)

11573/1673852 - 2023 - Understanding the molecular basis of folding cooperativity through a comparative analysis of a multidomain protein and its isolated domains

Santorelli, Daniele; Marcocci, Lucia; Pennacchietti, Valeria; Nardella, Caterina; Diop, Awa; Pietrangeli, Paola; Pagano, Livia; Toto, Angelo; Malagrino, Francesca; Gianni, Stefano - 01a Articolo in rivista

paper: THE JOURNAL OF BIOLOGICAL CHEMISTRY (American Society for Biochemistry and Molecular Biology:9650 Rockville Pike:Bethesda, MD 20814:(301)530-7145, EMAIL: asbmb@asbmb.faseb.org, INTERNET: http://www.faseb.org/asbmb, Fax: (301)571-1824) pp. 102983- - issn: 0021-9258 - wos: WOS:001009100600001 (3) - scopus: 2-s2.0-85149793931 (3)

11573/1662469 - 2022 - SH2 Domains: folding, binding and therapeutical approaches

Diop, Awa; Santorelli, Daniele; Malagrinò, Francesca; Nardella, Caterina; Pennacchietti, Valeria; Pagano, Livia; Marcocci, Lucia; Pietrangeli, Paola; Gianni, Stefano; Toto, Angelo - 01a Articolo in rivista

paper: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 15944- - issn: 1422-0067 - wos: WOS:000902565100001 (18) - scopus: 2-s2.0-85144969444 (21)

11573/1607647 - 2022 - On the effects of disordered tails, supertertiary structure and quinary interactions on the folding and function of protein domains

Malagrino, F.; Pennacchietti, V.; Santorelli, D.; Pagano, L.; Nardella, C.; Diop, A.; Toto, A.; Gianni, S. - 01a Articolo in rivista

paper: BIOMOLECULES (Basel: MDPI) pp. 209- - issn: 2218-273X - wos: WOS:000763931100001 (7) - scopus: 2-s2.0-85123285981 (7)

11573/1652072 - 2022 - Cryptic binding properties of a transient folding intermediate in a PDZ tandem repeat

Malagrinò, Francesca; Fusco, Giuliana; Pennacchietti, Valeria; Toto, Angelo; Nardella, Caterina; Pagano, Livia; De Simone, Alfonso; Gianni, Stefano - 01a Articolo in rivista

11573/1651291 - 2022 - Folding and binding mechanisms of the SH2 domain from Crkl

Nardella, Caterina; Toto, Angelo; Santorelli, Daniele; Pagano, Livia; Diop, Awa; Pennacchietti, Valeria; Pietrangeli, Paola; Marcocci, Lucia; Malagrinò, Francesca; Gianni, Stefano - 01a Articolo in rivista

paper: BIOMOLECULES (Basel: MDPI) pp. 1014- - issn: 2218-273X - wos: WOS:000846180000001 (5) - scopus: 2-s2.0-85137360513 (5)

11573/1657644 - 2022 - Exploring the effect of tethered domains on the folding of Grb2 protein

Pagano, Livia; Pennacchietti, Valeria; Diop, Awa; Santorelli, Daniele; Pietrangeli, Paola; Marcocci, Lucia; Nardella, Caterina; Malagrinò, Francesca; Toto, Angelo; Gianni, Stefano - 01a Articolo in rivista

paper: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (Attuale:ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, USA, NY, 10010-1710 Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445) pp. 109444- - issn: 0003-9861 - wos: WOS:000880049100004 (3) - scopus: 2-s2.0-85141826263 (4)

11573/1635685 - 2022 - Characterization of early and late transition states of the folding pathway of a SH2 domain

Toto, Angelo; Malagrinò, Francesca; Nardella, Caterina; Pennacchietti, Valeria; Pagano, Livia; Santorelli, Daniele; Diop, Awa; Gianni, Stefano - 01a Articolo in rivista

11573/1552783 - 2021 - Probing the effects of local frustration in the folding of a multidomain protein

Pagano, Livia; Malagrinò, Francesca; Visconti, Lorenzo; Troilo, Francesca; Pennacchietti, Valeria; Nardella, Caterina; Toto, Angelo; Gianni, Stefano - 01a Articolo in rivista

paper: JOURNAL OF MOLECULAR BIOLOGY (-LONDON, ENGLAND: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, -London; New York: Academic Press.) pp. 167087- - issn: 0022-2836 - wos: WOS:000672680300017 (6) - scopus: 2-s2.0-85107967375 (6)