VALERIA FEDELI

Dottoressa di ricerca

ciclo: XXXVI


supervisore: Mariano Bizzarri

Titolo della tesi: RESTORE OF STEROIDOGENIC PATHWAY UPON (IN VITRO AND IN VIVO) TREATMENT WITH MYO-INS AND D-CHIRO-INS IN PCOS-INDUCED MOUSE MODEL.

Polycystic ovary syndrome (PCOS) is a common metabolic dysfunction and heterogeneous endocrine disorder in women of reproductive age. Women with PCOS develop infertility and non-reproductive metabolic abnormalities, in particular, there is decrease of production of female hormones due to a lack/reduction of Aromatase inside the ovaries. There are several approaches used to restore hormonal balance, but in recent years a lot of attention has been focused on the treatments with natural molecules, myo-Inositol and D-chiro-Inositol. Experimental studies show that a correct treatment strategy could be a combination of both isomers. However, the correct formula is still a matter of debate. We tested the action of both isomers of inositol In vitro, with primary cultures of Granulosa and Theca isolated from the ovaries of PCOS induced mice, and In vivo through treatment of PCOS mice models. Different ratios of both isomers and different dietary supplement concentrations of myo-Ins and D-chiro-Ins were tested in primary culture in order to find the correct formula to use to reverse the molecular phenotype. Through In vitro studies we found that the ratio myo-Ins:D-chiro-Ins 40:1 with concentration of myo-Ins 0.5 mM and D-chiro-Ins 12.5 μM increases the expression of key enzymes involved in steroidogenesis pathway. After that, we increased the expression of enzyme of steroidogenic pathway and female hormone level in blood through In vivo treatment of PCOS mice model with the same ratio and concentration of In vitro studies.

Produzione scientifica

11573/1697351 - 2023 - Decreased fertility in female mice lacking urokinase plasminogen activator
Laurenzi, Gaia; Fedeli, Valeria; Canipari, Rita - 01a Articolo in rivista
rivista: REPRODUCTIVE BIOLOGY (Olsztyn: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences.) pp. - - issn: 1642-431X - wos: (0) - scopus: (0)

11573/1617836 - 2022 - Microgravity modifies the phenotype of fibroblast and promotes remodeling of the fibroblast–keratinocyte interaction in a 3D co-culture model
Fedeli, Valeria; Cucina, Alessandra; Dinicola, Simona; Fabrizi, Gianmarco; Catizone, Angiolina; Gesualdi, Luisa; Ceccarelli, Simona; Halim Harrath, Abdel; Alwasel, Saleh H.; Ricci, Giulia; Pedata, Paola; Bizzarri, Mariano; Monti, Noemi - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 1-22 - issn: 1422-0067 - wos: WOS:000762469000001 (10) - scopus: 2-s2.0-85124612813 (12)

11573/1682286 - 2022 - Paradoxical behavior of oncogenes undermines the somatic mutation theory
Monti, Noemi; Verna, Roberto; Piombarolo, Aurora; Querqui, Alessandro; Bizzarri, Mariano; Fedeli, Valeria - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. 1-15 - issn: 2218-273X - wos: WOS:000804903700001 (9) - scopus: 2-s2.0-85129196427 (10)

11573/1617951 - 2022 - Does Alpha-lipoic acid improve effects on polycystic ovary syndrome?
Simone Laganà, Antonio; Monti, Noemi; Fedeli, Valeria; Gullo, Giuseppe; Bizzarri, Mariano - 01a Articolo in rivista
rivista: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES (Roma: Verduci publisher) pp. 1241-1247 - issn: 2284-0729 - wos: WOS:000763332500024 (20) - scopus: 2-s2.0-85125795949 (23)

11573/1590058 - 2021 - Personalization of medical treatments in oncology: time for rethinking the disease concept to improve individual outcomes
Bizzarri, M.; Fedeli, V.; Monti, N.; Cucina, A.; Jalouli, M.; Alwasel, S. H.; Harrath, A. H. - 01g Articolo di rassegna (Review)
rivista: THE EPMA JOURNAL (Dordrecht : Springer Netherlands) pp. 1-14- - issn: 1878-5085 - wos: WOS:000705790900001 (8) - scopus: 2-s2.0-85116777794 (8)

11573/1545100 - 2021 - PCOS and inositols: controversial results and necessary clarifications. Basic differences between D-chiro and myo-inositol
Monastra, Giovanni; Vucenik, Ivana; Halim Harrath, Abdel; Alwasel, Saleh H.; Kamenov, Zdravko A.; Simone Laganà, Antonio; Monti, Noemi; Fedeli, Valeria; Bizzarri, Mariano - 01a Articolo in rivista
rivista: FRONTIERS IN ENDOCRINOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. - - issn: 1664-2392 - wos: WOS:000641265200001 (15) - scopus: 2-s2.0-85104685129 (22)

11573/1590060 - 2021 - Analytical performance of COVID-19 detection methods (RT-PCR): scientific and societal concerns
Verna, Roberto; Alallon, Walter; Murakami, Masami; Hayward, Catherine P. M.; Halim Harrath, Abdel; Alwasel, Saleh H.; Sumita, Nairo M.; Alatas, Ozkan; Fedeli, Valeria; Sharma, Praveen; Fuso, Andrea; Maria Capuano, Daniela; Capalbo, Maria; Angeloni, Antonio; Bizzarri, Mariano - 01g Articolo di rassegna (Review)
rivista: LIFE (Basel: MDPI) pp. 1-16 - issn: 2075-1729 - wos: WOS:000676692400001 (12) - scopus: 2-s2.0-85111244627 (13)

11573/1478972 - 2020 - New statistical RI index allow to better track the dynamics of COVID-19 outbreak in Italy
Bizzarri, M.; Di Traglia, M.; Giuliani, A.; Vestri, A.; Fedeli, V.; Prestininzi, A. - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. 1-13 - issn: 2045-2322 - wos: WOS:000603600300001 (5) - scopus: 2-s2.0-85097927684 (5)

11573/1500175 - 2020 - Complexity in biological organization: deconstruction (and subsequent restating) of key concepts
Bizzarri, Mariano; Naimark, Oleg; Nieto-Villar, José; Fedeli, Valeria; Giuliani, Alessandro - 01a Articolo in rivista
rivista: ENTROPY (Basel : MDPI, 1999-) pp. 1-15 - issn: 1099-4300 - wos: WOS:000567308300001 (17) - scopus: 2-s2.0-85090361624 (21)

11573/1499669 - 2020 - A challenging task—how to successfully separate theca and granulosa cells: a mandatory step for investigating ovary steroidogenesis
Fedeli, Valeria; Monti, Noemi; Canipari, Rita - 01a Articolo in rivista
rivista: ORGANISMS (Roma: Sapienza Università di Roma) pp. 86-89 - issn: 2532-5876 - wos: (0) - scopus: (0)

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