VALENTINA VAPORE

Dottoressa di ricerca

ciclo: XXXII



Titolo della tesi: Yeast as a tool to unveil the cellular and molecular mechanisms underlying neurodegeneration in dementia FENIB

The budding yeast Saccharomyces cerevisiae has been extensively used as a model system for understanding eukaryotic cellular processes. It is an established model organism for the analysis of evolutionarily conserved cellular pathways relevant for protein degradation, protein quality control systems, cell death and aging. In this context S. cerevisiae is an excellent candidate for studying neuropathies, such as FENIB (Familial Encephalopathy with Neuroserpin Inclusion Bodies), a rare neurodegenerative disorder with autosomal dominant inheritance. The dementia FENIB is caused by different mutations in neuroserpin (NS) gene that promote NS polymerization and its subsequent intracellular deposition, leading to a poorly understood cell toxicity. The severity of the disease phenotype depends on the specific mutation. We focused on studying one of the most severe forms of FENIB, due to the substitution of the glycine at position 392 with glutamic acid (G392E). To create a humanized yeast model for FENIB, the wild-type and the mutant variant (G392E) of the NS gene were cloned into yeast expression vectors and inserted in different yeast strains. In this study we reported data on NS expression and localization within the yeast cells. Moreover, we analysed the cytotoxic effect of NS with the aim to identify the molecular mechanisms underlying toxicity.

Produzione scientifica

  • 11573/624801 - 2014 - In vivo selection of JARID histone demethylases inhibitors and their use to enlighten the biological role of these enzymes in yeast and mammalian cells with focus on transcriptional regulation (04b Atto di convegno in volume)
    LICURSI, VALERIO; VAPORE, VALENTINA; PROIETTO, MARCO; BUFALIERI, FRANCESCA; RINALDI, TERESA; LA REGINA, GIUSEPPE; NEGRI, RODOLFO
  • 11573/766195 - 2014 - The cop9 signalosome is involved in the regulation of lipid metabolism and of transition metals uptake in S. cerevisiae (14s Intervento a convegno non pubblicato)
    CIRIGLIANO, ANGELA; LICURSI, VALERIO; RINALDI, TERESA; MATTEI, MARIA BENEDETTA; SERINO, GIOVANNA; VAPORE, VALENTINA; NEGRI, RODOLFO
  • 11573/850648 - 2015 - S.cerevisiae as a tool to select inhibitors of the deneddylating activity of the COP9 signalome Angela Cirigliano, Sergio Menta, Mattia Mori, Valerio Licursi, Svetlana Danovska, Valentina Vapore, Giovanna Serino, Elah Pick, Bruno Botta, Rodolfo Negri, and Teresa Rinaldi (04f Poster)
    CIRIGLIANO, ANGELA; MENTA, SERGIO; MORI, MATTIA; LICURSI, VALERIO; DANOVSKA, SVETLANA; VAPORE, VALENTINA; SERINO, GIOVANNA; BOTTA, BRUNO; NEGRI, RODOLFO; RINALDI, TERESA
  • 11573/1564262 - 2021 - Neuroserpin Inclusion Bodies in a FENIB Yeast Model (01a Articolo in rivista)
    VAPORE, VALENTINA; MIRANDA BANOS, MARIA ELENA; RINALDI, TERESA; MAZZONI, CRISTINA
  • 11573/1610070 - 2018 - Yeast as a tool to unveil the cellular and molecular mechanisms underlying neurodegeneration in FENIB disease (04d Abstract in atti di convegno)
    VAPORE, VALENTINA; STIRPE, MARIARITA; RINALDI, TERESA; MIRANDA BANOS, MARIA ELENA; MAZZONI, CRISTINA
  • 11573/1610071 - 2019 - Yeast response to the expression of human proteins involved in proteinopathies (04d Abstract in atti di convegno)
    VAPORE, VALENTINA; RINALDI, TERESA; MIRANDA BANOS, MARIA ELENA; MAZZONI, CRISTINA

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