Research: Targeting redox alterations and sensors in colorectal cancer
Personal data:
Name and Surname: Silvia Ripa
Date and place of birth: 24/11/1996, Roma (RM), Italy
Citizenship: Italian
Work address: Laboratory of Molecular Oncology (Department of Molecular
Medicine), Viale Regina Elena 291, 00161 Rome, Italy
Phone number: +39-06-49255132
Mail address: silvia.ripa@uniroma1.it
Education:
January-March 2023: Corso di formazione ‘sperimentazione preclinica e benessere animale’ moduli teorici e pratici per la funzione A, at ’Università di Roma La Sapienza, Roma (Italy)
November 2022: qualification to the profession of pharmacist and registration in the Register of Pharmacists
September 2015-January 2021: Master degree in Pharmacy and Medicine at La Sapienza
University of Rome, Rome (Italy).
Score: 110/110 cum laude.
Thesis title: “Il cancro della prostata resistente alla
castrazione: interazione tra il recettore per gli androgeni e
la via di segnale PI3K/Akt/mTOR ”.
Supervisor: Prof.ssa Ida Silvestri – Dept. of Molecular Medicine,
La Sapienza University of Rome, Rome (Italy)
September 2018- February 2019: Erasmus at Universitat de Barcelona, Barcelona
6 month of Erasmus+ project spent at 'Universitat de
Barcelona' doing laboratory activities of pharmacology,
pharmagnosy and plant phisiology.
September 2010- July 2015: High school diploma at Liceo Classico Giulio Cesare Roma
Research experience:
November 2021– Present: PhD Student in Molecular Medicine, Dept. of Molecular Medicine, Sapienza University, Rome (Italy).
Tutor: Prof. Gianluca Canettieri.
Field of interest: Targeting redox alterations and sensors in
colorectal cancer.
May 2021 – November 2021: Trainee at Dept of Molecular Medicine, Sapienza University of Rome (Italy).
Tutor: Prof. Gianluca Canettieri
August 2019 – January 2021: Trainee at Policlinico Umberto I Dept. of Molecular Medicine,
Sapienza University of Rome, Rome (Italy).
Tutor: Prof.ssa Ida Silvestri
Field of interest: interaction between PI3K/Akt/mTOR
pathway and prostate cancer
Technical skills and competences:
Cellular biology: cell culture techniques (animal and human cells), DNA and siRNA transfection/transduction, cell treatments, proliferation assay, cell viability and proliferation assay, drug-screening, proliferation and cellular vitality assay (MTT and TB), clonogenicity assay, production and use of lentiviral vectors.
Molecular Biology techniques: Plasmidic and genomic DNA extraction, RNA extraction, cloning, DNA mutagenesis, reverse transcription, PCR, quantitative real time PCR, luciferase reporter assays.
Analysis of proteins: Protein extraction and western blot analysis, immunoprecipitation.
Cytofluorometry techniques
Animal (scientific procedures): manipulation of mice and mice’s tissues, tail cutting, toe clipping.
Data analysis: Adobe Photoshop, Image J, Image Lab, GraphPad. Use of various DataBases and tools: NCBI, BLAST, GenScript, Uniprot, Biorender, STRING.
Congress partecipation:
September 2022: Poster presentation ‘Discovery of novel human lactate dehydrogenase inhibitors: Structure-based virtual screening studies and biological assessment’
Molecular pathology: from bench to bedside- SIPMeT Young Scientist
Meeting. Ancona (Italy)
September 2023: Poster presentation ‘Discovery of novel human lactate dehydrogenase inhibitors: Structure-based virtual screening studies and biological assessment’
Structure-based virtual screening studies and biological assessment’
General Pathology: the trunk of the tree of medicine Molecular
pathology- SIPMeT Young Scientist Meeting. Parma (Italy)
Publications:
- Di Magno L, Coluccia A, Bufano M, Ripa S, La Regina G, Nalli M, Di Pastena F, Canettieri G, Silvestri R, Frati L. Discovery of novel human lactate dehydrogenase inhibitors: Structure-based virtual screening studies and biological assessment. Eur J Med Chem. 2022;240.
- Nalli M, Di Magno L, Wen Y, Liu X, D'Ambrosio M, Puxeddu M, Parisi A, Sebastiani J, Sorato A, Coluccia A, Ripa S, Di Pastena F, Capelli D, Montanari R, Masci D, Urbani A, Naro C, Sette C, Orlando V, D'Angelo S, Biagioni S, Bigogno C, Dondio G, Pastore A, Stornaiuolo M, Canettieri G, Liu T, Silvestri R, La Regina G. Novel N-(Heterocyclylphenyl)benzensulfonamide Sharing an Unreported Binding Site with T-Cell Factor 4 at the β-Catenin Armadillo Repeats Domain as an Anticancer Agent. ACS Pharmacol Transl Sci. 2023 Jul 3;6(7):1087-1103.