Research: Dissecting the role of the Cullin3-RENKCTD11 E3 ubiquitin ligase complex in the control of the Hedgehog pathway and medulloblastoma tumorigenesis.
CURRENT POSITION
18/10/2021-on going: PhD Student in Molecular Medicine
Department of Molecular Medicine,Sapienza, University of Rome (Italy) Tutor: Prof.ssa Lucia Di Marcotullio
Field of interest: Study of ubiquitylation events in brain tumors
PREVIOUS RESEARCH EXPERIENCES
09/2020-18/10/2021: Trainee student
Department of Biology and Biotechnologies “C. Darwin”,Sapienza, University of Rome (Italy)
Tutor: Prof.ssa Lucia Piacentini
Thesis title: “Role of HP1 (Heterochromatin Protein 1) in epigenetic regulations of neural stem cells in Drosophila melanogaster”.
09/2020-18/10/2021: Trainee student
Department of Biology and Biotechnologies “C. Darwin”, Sapienza, University of Rome (Italy)
Tutor: Prof.ssa Lucia Piacentini in collaboration with Prof.ssa Luciana Mosca Project: “Neuroprotective role of curcumin, quercetin and resveratrol in the pathogenesis of Alzheimer’s disease in transgenic models of Drosophila melanogaster”
EDUCATION
2019-18/10/2021: MSc in Genomic, Industrial and Environmental Biotechnologies
Genomic curriculum
Department of Biology and Biotechnologies “C. Darwin”, Sapienza, University of Rome (Italy)
Tutor: Prof.ssa Lucia Piacentini Score: 110/110 cum laude
Thesis title: “Ruolo di HP1 (Heterochromatin Protein 1) nella regolazione epigenetica delle cellule staminali neurali in Drosophila melanogaster”.
Excellent graduate faculty of physical and natural mathematical sciences 2020-2021
2016-25/07/2019: Bachelor’s degree in Biological Sciences
Molecular and genetic curriculum
Department of Physiology, Sapienza University, Rome (Italy) Tutor: Prof.ssa Maria Egle De Stefano
Score: 101/110
Thesis title: “Ritmi circadiani e benessere psicofisico: un ruolo per gli astrociti”
Professional practice exam, Biologist (SEC. A)
November 2022 | Sapienza University, Rome (Italy)
Teaching Activity
Tutoring activities for the advanced analytical methodologies course
Faculty of Genomic, Industrial and Environmental Biotechnologies, Sapienza University, Dept. Biology and Biotechnology (Rome, Italy)
EXPERTISE AND SKILLS
Biochemistry/Genetics:
DNA, RNA, and proteins extraction from tissues/cell lysates
Agarose and Polyacrylamide Gel Electrophoresis
Blotting and immunoprecipitation techniques
In vivo ubiquitylation assays
Gene knock-down and silencing
Lentiviral virus preparation, titration, and infection
Bacterial transformation, Miniprep, Maxiprep
Fluorescence, light microscopy, Immunofluorescence and Confocal microscopy
Paraffin inclusion and immunohistochemistry
PCR and Quantitative Real Time PCR
Vector design: primers design, digestions, ligations, cloning
Biology:
Maintenance of eukaryotic, stem, and primary cells culture
Transfection and Electroporation
Proliferative, clonogenic, and apoptosis assays
IncuCyte® Live-Cell technology (including data analysis)
Animal Care:
Drosophila handle, restrain and breeding. Dissection.
Mice handle, restrain, and breeding
Subcutaneous and intraperitoneal injections
Surgical procedures: allograft and xenograft, heterotopic and orthotopic procedures
IT skills: Office suite; Adobe Photoshop; Image J; Image Lab; GraphPad. Use of various Databases and tools: NCBI, PDB, FlyBase, BLAST, GenScript.
Oral and poster presentations
- XVI FISV Congress, 3R: Research, Resilience and Reprise - Portici, Naple, Italy (14-16 Sept 2022) - Flash oral presentation and poster
- SIPMeT Congress 2022 "PATHOPHYSIOLOGY OF DISEASE" - Ancona, 22-24 Sept 2022 - Poster presentation
- SIrteps 3rd Meeting: Precision Medicine in the Era of Integrated Omics – Favignana, Trapani, Italy (17-20 May 2023) - Poster presentation
- National ABCD PhD Meeting and National Congress – Paestum, Salerno, Italy (20-23 Sept 2023) - Poster presentation
ABCD Travel Grant for ABCD National Congress 2023 (ABCD Scientific Committee)
- 35th AICCInternational Meeting 2023 – L’Aquila, Italy (04-06 Dec 2023) - Poster presentation
Best Poster Award by Journal of Experimental and Clinical Cancer Research (AICC Congress 2023)
Certifications
2023 Certification 24 CFU
2023 FELASA – Federation of European Laboratory Animal Science
Functions A, B, C, D (n. F023/09), Fondazione Santa Lucia IRCCS, Centro Europeo di Ricerca sul Cervello (CERC), Rome (Italy)
Publications:
Lospinoso Severini L, Loricchio E, Navacci S, […], SALL4 is a CRL3REN/KCTD11 substrate that drives Sonic Hedgehog-dependent medulloblastoma.
Cell Death Differ. 2023
doi: 10.1038/s41418-023-01246-6. Epub ahead of print.
PMID: 38062245.
Languages:
Italian: native
English: excellent level spoken and written
Farsi: basic knowledge