SEBASTIANO VACCARELLA

PhD Student

PhD program:: XXXIX
email: sebastiano.vaccarella@ifo.it
phone: 3209497320
building: UOC di Ricerca Traslazionale Oncologica presso IRCCS Regina Elena National Cancer Institute




supervisor: Dott. Giovanni Blandino
advisor: Dott. Giovanni Blandino
co-supervisor: Prof. Giuseppe Giannini

Research: TUMOR MICROENVIRONMENT: A CATALYZER OF TUMOR GROWTH, AGGRESSIVENESS AND INVASION

EDUCATION
[1212-2023 – 13-12-2023] OPERA PHENIX PLUS Revvity training course
[4-05-2023 – 5-05-2023] Scientific poster presentation presso il I Convegno Nazionale Società Organ-on-Chip
[11/2022 – Attuale] Master Universitario di II livello in Ricerca Clinica, open innovation e market access nell'era digitale (Università Campus Bio-Medico di Roma in collaborazione con Farmaindustria)
[10/2020 – 01/2023] Degree in Medical Biotechnology LM-9 (University of Rome)
[10/2017 – 10/2020] Degree in Biotechnology L-2 (University of Ferrara)

PREVIOUS RESEARCH EXPERIENCE:
[November 2023-Today] Researcher: PhD fellow in Molecular Medicine, University of Rome, (at Regina Elena National Cancer Institute, Translational Oncology Research Unit)
[December 2022-Today] Health Researcher in Translational Oncology Research Unit (head Dr. Giovanni Blandino) in Regina Elena National Cancer Institute, Rome, Italy
[April 2021- January 2022] Researcher in Translational Oncology Research Unit. Supervisor: Dr.ssa Valeria Canu, research field: Oncogenic role of SPAG5 in TNBC

LABORATORY SKILLS:
Cancer cell line and patient-derived 3D culture, Lipofectamine transfection, Colony assay, Serum, plasma, PBMC processing from whole blood, DNA/RNA/miRNA extraction from cell line, whole blood, organoids, fresh tumor and FFPE tissue, mRNA and miRNA retro-trascription, Polimerase Chain Reaction (PCR); Quantitave Real Time PCR (RT-qPCR), Immunofluorescense Assay, Chromatin Immunoprecipitation (ChiP), Western Blotting, Viability assay and IC50/EC50 analysis using PelkinElmer ENSPIRE, Drug Screening using Revvity OPERA PHENIX PLUS.

Research products

11573/1726729 - 2024 - Targeting of mutant-p53 and MYC as a novel strategy to inhibit oncogenic SPAG5 activity in triple negative breast cancer
Canu, Valeria; Vaccarella, Sebastiano; Sacconi, Andrea; Pulito, Claudio; Goeman, Frauke; Pallocca, Matteo; Rutigliano, Daniela; Lev, Sima; Strano, Sabrina; Blandino, Giovanni - 01a Articolo in rivista
paper: CELL DEATH & DISEASE (Nature Group) pp. - - issn: 2041-4889 - wos: WOS:001295046700001 (0) - scopus: 2-s2.0-85201702086 (1)

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