SEBASTIANO VACCARELLA

PhD Student

PhD program:: XXXIX
email: sebastiano.vaccarella@ifo.it
phone: 3209497320
building: UOC di Ricerca Traslazionale Oncologica presso IRCCS Regina Elena National Cancer Institute

supervisor: Dott. Giovanni Blandino
advisor: Dott. Giovanni Blandino
co-supervisor: Prof. Giuseppe Giannini

Research: TUMOR MICROENVIRONMENT: A CATALYZER OF TUMOR GROWTH, AGGRESSIVENESS AND INVASION

EDUCATION
[1212-2023 – 13-12-2023] OPERA PHENIX PLUS Revvity training course
[4-05-2023 – 5-05-2023] Scientific poster presentation presso il I Convegno Nazionale Società Organ-on-Chip
[11/2022 – Attuale] Master Universitario di II livello in Ricerca Clinica, open innovation e market access nell'era digitale (Università Campus Bio-Medico di Roma in collaborazione con Farmaindustria)
[10/2020 – 01/2023] Degree in Medical Biotechnology LM-9 (University of Rome)
[10/2017 – 10/2020] Degree in Biotechnology L-2 (University of Ferrara)

PREVIOUS RESEARCH EXPERIENCE:
[November 2023-Today] Researcher: PhD fellow in Molecular Medicine, University of Rome, (at Regina Elena National Cancer Institute, Translational Oncology Research Unit)
[December 2022-Today] Health Researcher in Translational Oncology Research Unit (head Dr. Giovanni Blandino) in Regina Elena National Cancer Institute, Rome, Italy
[April 2021- January 2022] Researcher in Translational Oncology Research Unit. Supervisor: Dr.ssa Valeria Canu, research field: Oncogenic role of SPAG5 in TNBC

LABORATORY SKILLS:
Cancer cell line and patient-derived 3D culture, Lipofectamine transfection, Colony assay, Serum, plasma, PBMC processing from whole blood, DNA/RNA/miRNA extraction from cell line, whole blood, organoids, fresh tumor and FFPE tissue, mRNA and miRNA retro-trascription, Polimerase Chain Reaction (PCR); Quantitave Real Time PCR (RT-qPCR), Immunofluorescense Assay, Chromatin Immunoprecipitation (ChiP), Western Blotting, Viability assay and IC50/EC50 analysis using PelkinElmer ENSPIRE, Drug Screening using Revvity OPERA PHENIX PLUS.

Research products

11573/1740275 - 2025 - MicroRNA-mediated PTEN downregulation as a novel non-genetic mechanism of acquired resistance to PI3Kα inhibitors of head & neck squamous cell carcinoma

Pulito, C.; Vaccarella, S.; Palcau, A. C.; Ganci, F.; Brandi, R.; Frascolla, C.; Sacconi, A.; Canu, V.; Benedetti, A.; De Pascale, V.; Donzelli, S.; Fisch, A. -S.; Manciocco, V.; Covello, R.; Pimpinelli, F.; Morrone, A.; Fazi, F.; Pellini, R.; Muti, P.; Meens, J.; Karamboulas, C.; Nichols, A. C.; Strano, S.; Klinghammer, K.; Tinhofer, I.; Ailles, L.; Fontemaggi, G.; Blandino, G. - 01a Articolo in rivista

paper: DRUG RESISTANCE UPDATES (W B Saunders Company:Fulfillment Department, The Curtis Center, Independence Square West:Philadelphia, PA 19106:(800)654-2452, (215)238-7800, EMAIL: wbspcs@harcourt.com, INTERNET: http://elsevierhealth.com, Fax: (215)238-6445) pp. 1-17 - issn: 1368-7646 - wos: WOS:001496049400002 (0) - scopus: 2-s2.0-105005357808 (0)

11573/1726729 - 2024 - Targeting of mutant-p53 and MYC as a novel strategy to inhibit oncogenic SPAG5 activity in triple negative breast cancer

Canu, Valeria; Vaccarella, Sebastiano; Sacconi, Andrea; Pulito, Claudio; Goeman, Frauke; Pallocca, Matteo; Rutigliano, Daniela; Lev, Sima; Strano, Sabrina; Blandino, Giovanni - 01a Articolo in rivista

paper: CELL DEATH & DISEASE (Nature Group) pp. - - issn: 2041-4889 - wos: WOS:001295046700001 (4) - scopus: 2-s2.0-85201702086 (4)