Sebastian Luca D'Addario

PhD Graduate

PhD program:: XXXIV



Thesis title: Early life adversities shape physiological response of VTA dopaminergic neurons to positive and negative stimuli and behavior in adulthood

Exposure to aversive events during sensitive developmental periods can affect the preferential coping strategy adopted by individuals later in life, leading to stress-related psychiatric disorders, such as depression and addiction or, alternatively, increasing resilience to stress effects. We have previously shown that interfering with the development of mother-pups bond by the Repeated Cross Fostering (RCF) protocol induces resilience to depression-like phenotype, and increases susceptibility to cocaine effects in adult C57BL/6J female mice. Here, we used patch-clamp recording in midbrain slice combined with both in vivo and ex vivo pharmacology approaches to test our hypothesis of a link between electrophysiological modifications of dopaminergic neurons in the intermediate Ventral Tegmental Area (VTA) of RCF animals and their behavior. We found reduced hyperpolarization-activated (Ih) cation current amplitude and evoked firing in VTA dopaminergic neurons from both young and adult RCF female mice. In vivo pharmacological manipulation of the VTA neurons Ih current reverted the pro-resilient to depression phenotype and reduced sensitivity to cocaine effects in adult early-stressed mice or mimicked behavioral resilience to depression in adult control animals. However, ih current manipulation in control mice was not able to increase the sensitivity to cocaine effects. This is the first evidence showing how pro-resilience to depression behavior and susceptibility to cocaine induced by early events are linked to a long-lasting reduction of Ih current and excitability in VTA dopaminergic neurons.

Research products

11573/1688725 - 2023 - A mouse model of the 3-hit effects of stress: Genotype controls the effects of life adversities in females
Babicola, Lucy; Mancini, Camilla; Riccelli, Cristina; Di Segni, Matteo; Passeri, Alice; Municchi, Diana; D'addario, Sebastian Luca; Andolina, Diego; Cifani, Carlo; Cabib, Simona; Ventura, Rossella - 01a Articolo in rivista
paper: PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY (Pergamon Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. 110842- - issn: 0278-5846 - wos: WOS:001086135800001 (0) - scopus: 2-s2.0-85169848092 (0)

11573/1659983 - 2023 - The long-lasting effects of early life adversities are sex dependent: The signature of miR-34a
D'addario, Sebastian Luca; Municchi, Diana; Mancini, Camilla; Ielpo, Donald; Babicola, Lucy; Di Segni, Matteo; Lo Iacono, Luisa; Ferlazzo, Fabio; Cifani, Carlo; Andolina, Diego; Ventura, Rossella - 01a Articolo in rivista
paper: JOURNAL OF AFFECTIVE DISORDERS (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. - - issn: 0165-0327 - wos: WOS:000896745200005 (2) - scopus: 2-s2.0-85142413923 (2)

11573/1658988 - 2023 - Morpho-Functional Changes of Nigral Dopamine Neurons in an α-Synuclein Model of Parkinson's Disease
Ledonne, Ada; Massaro Cenere, Mariangela; Paldino, Emanuela; D'angelo, Vincenza; D'addario, Sebastian Luca; Casadei, Nicolas; Nobili, Annalisa; Berretta, Nicola; Fusco, Francesca R; Ventura, Rossella; Sancesario, Giuseppe; Guatteo, Ezia; Mercuri, Nicola Biagio - 01a Articolo in rivista
paper: MOVEMENT DISORDERS (-WILEY-LISS, DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, USA, NJ, 07030 -Lippincott, Williams & Wilkins:530 Walnut Street:Philadelphia, PA 19106:(800)638-3030, (301)223-2300, EMAIL: orders@lww.com, INTERNET: http://www.lww.com, Fax: (301)223-2320, (301)223-2320) pp. - - issn: 0885-3185 - wos: WOS:000880202700001 (3) - scopus: 2-s2.0-85141678685 (4)

11573/1671670 - 2023 - Chemogenetic rectification of the inhibitory tone onto hippocampal neurons reverts autistic-like traits and normalizes local expression of estrogen receptors in the Ambra1+/- mouse model of female autism
Pignataro, Annabella; Krashia, Paraskevi; De Introna, Margherita; Nobili, Annalisa; Sabetta, Annamaria; Stabile, Francesca; La Barbera, Livia; D'addario, Sebastian Luca; Ventura, Rossella; Cecconi, Francesco; D’Amelio, Marcello; Ammassari-Teule, Martine - 01a Articolo in rivista
paper: TRANSLATIONAL PSYCHIATRY (New York, NY : Nature Pub. Group-Springer Nature) pp. 63- - issn: 2158-3188 - wos: WOS:000935961000001 (2) - scopus: 2-s2.0-85148384111 (3)

11573/1652722 - 2022 - A multi-expert ensemble system for predicting Alzheimer transition using clinical features
Merone, M; D'addario, Sl; Mirino, P; Bertino, F; Guariglia, C; Ventura, R; Capirchio, A; Baldassarre, G; Silvetti, M; Caligiore D., A - 01a Articolo in rivista
paper: BRAIN INFORMATICS (Berlin; Heidelberg: Springer, 2014) pp. 1-11 - issn: 2198-4026 - wos: (0) - scopus: 2-s2.0-85137599645 (3)

11573/1487835 - 2021 - Long term effects of early life stress on HPA circuit in rodent models
Babicola, L.; Ventura, R.; D'addario, S. L.; Ielpo, D.; Andolina, D.; Di Segni, M. - 01a Articolo in rivista
paper: MOLECULAR AND CELLULAR ENDOCRINOLOGY (Elsevier Science Ireland Limited:PO Box 85, Limerick Ireland:011 353 61 709600, 011 353 61 61944, EMAIL: usinfo-f@elsevier.com, INTERNET: http://www.elsevier.com, Fax: 011 353 61 709114) pp. - - issn: 0303-7207 - wos: WOS:000605607800011 (9) - scopus: 2-s2.0-85098095336 (11)

11573/1542738 - 2021 - Resilience to anhedonia-passive coping induced by early life experience is linked to a long-lasting reduction of Ih current in VTA dopaminergic neurons: altered Ih current in the VTA of adult resilient-to-depression mice
D'addario, S. L.; Di Segni, M.; Ledonne, A.; Piscitelli, R.; Babicola, L.; Martini, A.; Spoleti, E.; Mancini, C.; Ielpo, D.; D'amato, F. R.; Andolina, D.; Ragozzino, D.; Mercuri, N. B.; Cifani, C.; Renzi, M.; Guatteo, E.; Ventura, R. - 01a Articolo in rivista
paper: NEUROBIOLOGY OF STRESS (New York : Elsevier Inc.) pp. - - issn: 2352-2895 - wos: WOS:000651603900003 (10) - scopus: 2-s2.0-85104148559 (11)

11573/1542950 - 2021 - MicroRNA-34a regulates 5-HT2C expression in dorsal raphe and contributes to the anti-depressant-like effect of fluoxetine
Lo Iacono, L.; Ielpo, D.; Parisi, C.; Napoli, G.; Accoto, A.; Di Segni, M.; Babicola, L.; D'addario, S. L.; Guzzo, S. M.; Pascucci, T.; Ventura, R.; Andolina, D. - 01a Articolo in rivista
paper: NEUROPHARMACOLOGY (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. - - issn: 0028-3908 - wos: WOS:000663459200006 (9) - scopus: 2-s2.0-85104079200 (10)

11573/1574955 - 2021 - Early life adversity affecting the attachment bond alters ventral tegmental area transcriptomic patterning and behavior almost exclusively in female mice
Lo Iacono, Luisa; Mancini, Camilla; Babicola, Lucy; Pietrosanto, Marco; Di Segni, Matteo; D'addario, Sebastian Luca; Municchi, Diana; Ielpo, Donald; Pascucci, Tiziana; Cabib, Simona; Ferlazzo, Fabio; D'amato, Francesca R.; Andolina, Diego; Helmer-Citterich, Manuela; Cifanic, Carlo; Ventura, Rossella - 01a Articolo in rivista
paper: NEUROBIOLOGY OF STRESS (New York : Elsevier Inc.) pp. - - issn: 2352-2895 - wos: WOS:000709723500003 (5) - scopus: 2-s2.0-85119379843 (5)

11573/1596916 - 2021 - Long-term depression of striatal DA release induced by mGluRs via sustained hyperactivity of local cholinergic interneurons
Mercuri, Nicola B; Federici, Mauro; Rizzo, Francesca Romana; Maugeri, Lorenzo; D'addario, Sebastian L; Ventura, Rossella; Berretta, Nicola - 01a Articolo in rivista
paper: FRONTIERS IN CELLULAR NEUROSCIENCE (Lausanne (PO Box 110, 1015) : Frontiers Research Foundation, 2007-) pp. - - issn: 1662-5102 - wos: WOS:000730717100001 (3) - scopus: 2-s2.0-85121439302 (3)

11573/1385624 - 2020 - RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress
Babicola, L; Pietrosanto, M; Ielpo, D; D'addario, Sl; Cabib, S; Ventura, R; Ferlazzo, F; Helmer-Citterich, M; Andolina, D; Lo Iacono, L. - 01a Articolo in rivista
paper: BRAIN RESEARCH (Amsterdam; Lausanne; New York; Oxford; Shannon; Tokyo: Elsevier) pp. - - issn: 0006-8993 - wos: WOS:000524457100010 (4) - scopus: 2-s2.0-85081672685 (4)

11573/1385627 - 2020 - Xlr4 as a new candidate gene underlying vulnerability to cocaine effects
Di Segni, M.; D'addario, S. L.; Babicola, L.; Ielpo, D.; Lo Iacono, L.; Andolina, D.; Accoto, A.; Luchetti, A.; Mancini, C.; Parisi, C.; D'onofrio, M.; Arisi, I.; Brandi, R.; Pascucci, T.; Cifani, C.; D'amato, F. R.; Ventura, R. - 01a Articolo in rivista
paper: NEUROPHARMACOLOGY (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. - - issn: 0028-3908 - wos: WOS:000528256100036 (3) - scopus: 2-s2.0-85081004285 (3)

11573/1307879 - 2020 - MicroRNA-34a regulates the depression-like behavior in mice by modulating the expression of target genes in the Dorsal Raphè
Lo Iacono, Luisa; Ielpo, Donald; Accoto, Alessandra; Di Segni, Matteo; Babicola, Lucy; D'addario, Sebastian Luca; Ferlazzo, Fabio; Pascucci, Tiziana; Ventura, Rossella; Andolina, Diego - 01a Articolo in rivista
paper: MOLECULAR NEUROBIOLOGY (Humana Press Incorporated:999 Riverview Drive, Suite 208:Totowa, NJ 07512:(973)256-1699, EMAIL: lglasner@humanapr.com, INTERNET: http://www.humanapress.com, Fax: (973)256-8341) pp. - - issn: 0893-7648 - wos: WOS:000515028000017 (16) - scopus: 2-s2.0-85071772292 (19)

11573/1291946 - 2019 - Sex-dependent effects of early unstable post-natal environment on response to positive and negative stimuli in adult mice
Di Segni, M; Andolina, D; D'addario, Sl; Babicola, L; Ielpo, D; Luchetti, A; Pascucci, T; Lo Iacono, L; D'amato, Fr; Ventura, R. - 01a Articolo in rivista
paper: NEUROSCIENCE (Oxford: Elsevier Ltd. Oxford; New York: Pergamon Press) pp. 1-10 - issn: 0306-4522 - wos: WOS:000476866600001 (12) - scopus: 2-s2.0-85067853998 (14)

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