SARA GRASSI

Dottoressa di ricerca

ciclo: XXXIII

Titolo della tesi: Dupilumab e colonizzazione microbica cutanea: analisi fenotipica e molecolare di Staphylococcus aureus e di stafilococchi coagulasi negativi in pazienti affetti da dermatite atopica

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease whose pathogenesis is still not completely understood. Recent evidences suggest that impaired barrier integrity associated with dysbiosis play a key role in AD. Staphylococcus aureus colonization has been associated with increased inflammation and disease severity. The aim of the study is to characterize S. aureus strains involved in the acute phase of the disease and to isolate and identify Coagulase Negative Staphylococci (CoNS) able to control/inhibit the skin colonization by S. aureus. Methods: In this prospective study 20 adult Caucasian with moderate to severe AD patients and 20 healthy controls will be enrolled. For each patient, three swabs collected from nose, lesional and nonlesional skin were performed before start treatment with dupilumab (flare phase) and at the clinical remission (post-flare) of disease in order to perform phenotypical and molecular analyses (cultural examination; Maldi-Tof mass spectrometry; RAPD-PCR) of resident microbial species. Results: We report preliminary results of the study. S. aureus was hydentified in all AD patients, and only in 10% oh healty controls. At flare phase, high load of S. aureus (104-105 UFC/mL) strong biofilm producers has been in all samples collected by lesional skin, significantly higer when compared to non lesional skin and to healty controls (103 UFC/ml, p<0.05). At post-flare, a significantly lower S. aureus load has been found in all samples associated with higher load of S. epidermidis capable of inhibiting S. aureus biofilm production. Conclusion: In acute AD patients there is a high bacterial load of S. aureus which also has a strong production of biofilm that offers the microorganism the ability to cooperate and adapt to a hostile and highly competitive environment. Dupilumab therapy, while improving the clinical signs of the disease, does not allow the complete eradication of S. aureus, however it is associated with a recolonization with new strains, especially of S. epidermidis.

Produzione scientifica

11573/970891 - 9999 - Unusual patterns of presentation of frontal firbosing alopecia: a clinical and trichoscopic analysis of 98 patients

Rossi, Alfredo; Grassi, Sara; Caterina, Fortuna Maria; Garelli, Valentina; Pranteda, Giulia; Caro, Gemma; Carlesimo, Marta - 01a Articolo in rivista

rivista: JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY (Mosby Year Book Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)654-2452, (407)345-4000, EMAIL: hhspcs@harcourt.com, INTERNET: http://www.mosby.com, Fax: (407)363-9661, (314)432-1380) pp. .--.- - issn: 0190-9622 - wos: (0) - scopus: (0)

11573/1687914 - 2023 - Atopic dermatitis-derived Staphylococcus aureus strains: what makes them special in the interplay with the host

Conte, Antonietta Lucia; Brunetti, Francesca; Marazzato, Massimiliano; Longhi, Catia; Maurizi, Linda; Raponi, Giammarco; Palamara, Anna Teresa; Grassi, Sara; Conte, Maria Pia - 01a Articolo in rivista

rivista: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY (Lausanne : Frontiers Media SA, 2011-) pp. 1-16 - issn: 2235-2988 - wos: WOS:001018350400001 (11) - scopus: 2-s2.0-85163744339 (9)

11573/1657631 - 2022 - A possible link between gut microbiome composition and cardiovascular comorbidities in psoriatic patients

Valentini, Virginia; Silvestri, Valentina; Bucalo, Agostino; Marraffa, Federica; Risicato, Maria; Grassi, Sara; Pellacani, Giovanni; Ottini, Laura; Richetta, Antonio Giovanni - 01a Articolo in rivista

rivista: JOURNAL OF PERSONALIZED MEDICINE (Basel: MDPI AG, 2011-) pp. - - issn: 2075-4426 - wos: WOS:000833804400001 (7) - scopus: 2-s2.0-85137220471 (7)

11573/1718996 - 2021 - Frontal fibrosing alopecia and genital Lichen sclerosus: Single‐center experience

Grassi, Sara; Tadiotto Cicogna, Giulia; Magri, Francesca; Caterina Fortuna, Maria; Caro, Gemma; Pernazza, Angelina; Soda, Giuseppe; Miraglia, Emanuele; Giustini, Sandra; Carlesimo, Marta; Rossi, Alfredo - 01a Articolo in rivista

rivista: JOURNAL OF COSMETIC DERMATOLOGY (Blackwell Science.) pp. 615-620 - issn: 1473-2130 - wos: WOS:000557273300001 (8) - scopus: 2-s2.0-85089082205 (9)

11573/1423012 - 2021 - Frontal fibrosing alopecia and genital lichen sclerosus: single-center experience

Grassi, Sara; Tadiotto Cicogna, Giulia; Magri, Francesca; Fortuna, Maria Caterina; Caro, Gemma; Pernazza, Angelina; Soda, Giuseppe; Miraglia, Emanuele; Giustini, Sandra; Carlesimo, Marta; Rossi, Alfredo - 01a Articolo in rivista

rivista: JOURNAL OF COSMETIC DERMATOLOGY (Oxford : Blackwell Science.) pp. 615-620 - issn: 1473-2165 - wos: WOS:000557273300001 (8) - scopus: 2-s2.0-85089082205 (9)

11573/1583161 - 2021 - Ustekinumab for the treatment of moderate-to-severe plaque psoriasis in a solid organ transplanted recipient: a case report

Richetta, A.; Marraffa, F.; Grassi, S.; Aquila, E.; Dybala, A.; Aprile, F.; Merli, M. - 01f Lettera, Nota

rivista: AUSTRALASIAN JOURNAL OF DERMATOLOGY (Blackwell Science Asia Party Limited:550 Swanston Street, Carlton VIC 3053 Australia:011 61 3 83591011, EMAIL: info@blackwell-science-asia.com, INTERNET: http://www.blackwell-science.com, Fax: 011 61 3 83591120) pp. e442-e443 - issn: 0004-8380 - wos: WOS:000621800000001 (3) - scopus: 2-s2.0-85101609353 (3)

11573/1500906 - 2021 - Gut microbiome profile in psoriatic patients treated and untreated with biologic therapy

Valentini, V.; Silvestri, V.; Marraffa, F.; Greco, G.; Bucalo, A.; Grassi, S.; Gagliardi, A.; Mazzotta, A.; Ottini, L.; Richetta, A. G. - 01a Articolo in rivista

rivista: JOURNAL OF DERMATOLOGY (Nihon Hifuka Gakkai:(Japanese Dermatological Association), Taisei Building, 3-14-10 Hongo, Bunkyoku Tokyo 113 Japan) pp. - - issn: 0385-2407 - wos: WOS:000612550800001 (15) - scopus: 2-s2.0-85099821795 (15)

11573/1450800 - 2020 - Paradoxical psoriasis induced by Anti-TNFα treatment: Evaluation of disease-specific clinical and genetic markers

Bucalo, A.; Rega, F.; Zangrilli, A.; Silvestri, V.; Valentini, V.; Scafetta, G.; Marraffa, F.; Grassi, S.; Rogante, E.; Piccolo, A.; Cucchiara, S.; Viola, F.; Bianchi, L.; Ottini, L.; Richetta, A. - 01a Articolo in rivista

rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel (Matthaeustrasse 11) : Molecular Diversity Preservation International MDPI) pp. - - issn: 1661-6596 - wos: WOS:000588902800001 (19) - scopus: 2-s2.0-85093960459 (25)

11573/1445890 - 2020 - Increased intestinal barrier permeability in patients with moderate to severe plaque-type psoriasis

Richetta, A. G.; Grassi, S.; Moliterni, E.; Chello, C.; Calvieri, C.; Carnevale, R.; Peruzzi, M.; Violi, F.; Calvieri, S. - 01f Lettera, Nota

rivista: JOURNAL OF DERMATOLOGY (Nihon Hifuka Gakkai:(Japanese Dermatological Association), Taisei Building, 3-14-10 Hongo, Bunkyoku Tokyo 113 Japan) pp. e366-e368 - issn: 0385-2407 - wos: WOS:000558591600001 (8) - scopus: 2-s2.0-85089312876 (9)

11573/1306824 - 2019 - Recurrent erythema multiforme major in an 8-year-old patient with recurrence of herpetic gingivostomatitis and HLA-B*5801 haplotype: A causal or casual relationship?

Grassi, S.; Bossi, G.; Croci, G. A.; Brazzelli, V. - 01a Articolo in rivista

rivista: ‫JAAD CASE REPORTS ([New York] : Elsevier) pp. 617-620 - issn: 2352-5126 - wos: (0) - scopus: 2-s2.0-85068755760 (1)

11573/1304460 - 2019 - Regression of benign melanocytic nevi ipilimumab-induced in an adult patient affected by metastatic melanoma: is it a sign of response to therapy?

Grassi, Sara; Corsetti, Paola; Moliterni, Elisa; Calvieri, Stefano - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. - - issn: 1827-1820 - wos: WOS:000762123700042 (1) - scopus: 2-s2.0-85124444191 (1)

11573/1304464 - 2019 - Telangiectasia macularis eruptiva perstans: a neglected type of mastocytosis with exclusively cutaneous involvement? A case series

Michelerio, A.; Grassi, S.; Elena, C.; Croci, G. A.; Boveri, E.; Bossi, G.; Brazzelli, V. - 01a Articolo in rivista

rivista: EUROPEAN JOURNAL OF DERMATOLOGY (Montrouge: John Libbey eurotext) pp. 174-178 - issn: 1167-1122 - wos: WOS:000468423400007 (1) - scopus: 2-s2.0-85066421168 (2)

11573/1335149 - 2019 - The barrier beyond the skin

Moliterni, E; Paolino, G; Richetta, Ag; Grassi, S; Calvieri, S - 01m Editorial/Introduzione in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. - - issn: 1827-1820 - wos: WOS:000631837900002 (3) - scopus: 2-s2.0-85105573709 (3)

11573/1255202 - 2019 - Alopecia neoplastica as a sign of visceral malignancies: a systematic review

Paolino, G.; Pampena, R.; Grassi, S.; Mercuri, S. R.; Cardone, M.; Corsetti, P.; Moliterni, E.; Muscianese, M.; Rossi, A.; Frascione, P.; Longo, C. - 01g Articolo di rassegna (Review)

rivista: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. 1020-1028 - issn: 0926-9959 - wos: WOS:000468972400006 (14) - scopus: 2-s2.0-85062795588 (16)

11573/1305702 - 2019 - MiRNAs as potential prognostic biomarkers for metastasis in thin and thick primary cutaneous melanomas

Valentini, Virginia; Zelli, Veronica; Gaggiano, Emanuela; Silvestri, Valentina; Rizzolo, Piera; Bucalo, Agostino; Calvieri, Stefano; Grassi, Sara; Frascione, Pasquale; Pietro, Donati; Soda, Giuseppe; Ottini, Laura; Richetta, Antonio Giovanni - 01a Articolo in rivista

rivista: ANTICANCER RESEARCH (Kapandriti: International Institute of Anticancer Research, 1995 Earliest publisher: Καπανδρίτι: Ιωάννης Γ. Δεληνάσιος, 1981-1995 Kapandriti: Iōannīs G. Delīnasios, 1981-1995) pp. 4085-4093 - issn: 0250-7005 - wos: WOS:000482701400014 (12) - scopus: 2-s2.0-85070682011 (11)

11573/1194778 - 2018 - Ficoll-hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal residual disease analysis

Genuardi, E.; Barbero, D.; Dogliotti, I.; Mantoan, B.; Drandi, D.; Gambella, M.; Zaccaria, G. M.; Monitillo, L.; Della Starza, I.; Cavalli, M.; De Novi, L. A.; Ciabatti, E.; Grassi, S.; Gazzola, A.; Mannu, C.; Del Giudice, I.; Galimberti, S.; Agostinelli, C.; Piccaluga, P. P.; Ladetto, M.; Ferrero, S. - 01a Articolo in rivista

rivista: INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY (Oxford : Blackwell, 2007-) pp. 201-208 - issn: 1751-5521 - wos: WOS:000427250400021 (6) - scopus: 2-s2.0-85037660773 (6)

11573/1134485 - 2018 - Peculiar hypertrichosis in a patient affected by Frontal fibrosing alopecia with pseudo "fringe sign"

Grassi, Sara; Carlesimo, Marta; Fortuna, Maria C; Rossi, Alfredo - 01f Lettera, Nota

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. - - issn: 1827-1820 - wos: WOS:000548538600020 (1) - scopus: 2-s2.0-85087680917 (1)

11573/1109788 - 2018 - Incomplete excision of basal cell carcinoma of the head region: analysis of 724 consecutive cases

Grassi, Sara; Merlino, Mario; Rosso, Renato; Borroni, Giovanni - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. 435-437 - issn: 1827-1820 - wos: WOS:000441204800018 (0) - scopus: 2-s2.0-85047362061 (1)

11573/1134491 - 2018 - Monitoring chemotherapy-induced alopecia with trichoscopy

Rossi, Alfredo; Fortuna, Maria Caterina; Caro, Gemma; Cardone, Michele; Garelli, Valentina; Grassi, Sara; Carlesimo, Marta - 01a Articolo in rivista

rivista: JOURNAL OF COSMETIC DERMATOLOGY (Blackwell Science.) pp. - - issn: 1473-2130 - wos: WOS:000468038000023 (21) - scopus: 2-s2.0-85050812528 (23)

11573/1109790 - 2017 - Genetic counselling and high-penetrance susceptibility gene analysis reveal the novel CDKN2A p.D84V (c.251A>T) mutation in melanoma-prone families from Italy

Borroni, Riccardo G.; Manganoni, Ausilia M.; Grassi, Sara; Grasso, Maurizia; Diegoli, Marta; Giorgianni, Carmela; Favalli, Valentina; Pavoni, Laura; Cespa, Maddalena; Arbustini, Eloisa - 01a Articolo in rivista

rivista: MELANOMA RESEARCH (Lippincott, Williams & Wilkins:530 Walnut Street:Philadelphia, PA 19106:(800)638-3030, (301)223-2300, EMAIL: orders@lww.com, INTERNET: http://www.lww.com, Fax: (301)223-2320, (301)223-2320) pp. 97-103 - issn: 0960-8931 - wos: WOS:000395554200004 (1) - scopus: 2-s2.0-85008395771 (1)

11573/1095998 - 2017 - Unusual patterns of presentation of frontal fibrosing alopecia: A clinical and trichoscopic analysis of 98 patients

Rossi, Alfredo; Grassi, Sara; Fortuna, Maria Caterina; Garelli, Valentina; Pranteda, Giulia; Caro, Gemma; Carlesimo, Marta - 01a Articolo in rivista

11573/1109784 - 2017 - Pregnancy outcome after rituximab treatment before conception in patients affected by severe pemphigus vulgaris/superficialis

Vassallo, C.; Grassi, S.; Tagliabue, E.; Piccolo, A.; Brazzelli, V. - 01a Articolo in rivista

rivista: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. e331-e333 - issn: 0926-9959 - wos: WOS:000406075900015 (6) - scopus: 2-s2.0-85012289013 (7)

11573/1109792 - 2016 - Involvement of dermal microvascular basement membrane in senile purpura: quantitative immunohistochemical study

Borroni, R. G.; Grassi, S.; Concardi, M.; Agozzino, M.; Caspani, C.; Giordano, C.; Vignini, M.; Arbustini, E. - 01a Articolo in rivista

rivista: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. e63-e65 - issn: 0926-9959 - wos: WOS:000386688300021 (4) - scopus: 2-s2.0-84945395665 (5)

11573/1109794 - 2016 - Narrow-band UVB phototherapy and psoralen–ultraviolet A photochemotherapy in the treatment of cutaneous mastocytosis: a study in 20 patients

Brazzelli, Valeria; Grassi, Sara; Merante, Serena; Grasso, Vincenzo; Ciccocioppo, Rachele; Bossi, Grazia; Borroni, Giovanni - 01a Articolo in rivista

rivista: PHOTODERMATOLOGY, PHOTOIMMUNOLOGY & PHOTOMEDICINE (Blackwell Munksgaard:PO Box 2148, Periodicals Department, DK-1016 Copenhagen K Denmark:011 45 33 755913, EMAIL: agentservices@oxon.blackwellpublishing.com, INTERNET: http://www.blackwellmunksgaard.com, Fax: 011 45 77 333377) pp. 238-246 - issn: 0905-4383 - wos: WOS:000388315900001 (24) - scopus: 2-s2.0-84979502123 (24)

11573/1109802 - 2015 - Vasculitides with cutaneous expression in children: clinico-pathological correlations

Borroni, G; Grassi, S; Carugno, A - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. 51-71 - issn: 1827-1820 - wos: WOS:000352038200007 (1) - scopus: 2-s2.0-84942428182 (2)

11573/1109798 - 2015 - Erythema ab igne induced by laptop computer: an emerging disease among adolescents?

Brazzelli, V; Grassi, S; Barruscotti, S; Croci, G; Borroni, G - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino : Edizioni Minerva Medica) pp. - - issn: 1827-1820 - wos: (0) - scopus: (0)

11573/1109804 - 2015 - Adult-onset tufted angiomas associated with an arteriovenous malformation in a renal transplant recipient: Case report and review of the literature

Grassi, Sara; Carugno, Andrea; Vignini, Mariadelaide; Rosso, Renato; Borroni, Riccardo G. - 01a Articolo in rivista

rivista: AMERICAN JOURNAL OF DERMATOPATHOLOGY (Lippincott, Williams & Wilkins:530 Walnut Street:Philadelphia, PA 19106:(800)638-3030, (301)223-2300, EMAIL: orders@lww.com, INTERNET: http://www.lww.com, Fax: (301)223-2320, (301)223-2320) pp. 162-165 - issn: 0193-1091 - wos: WOS:000348597000015 (3) - scopus: 2-s2.0-84924050146 (2)

11573/1109812 - 2014 - Glomuvenous malformations with smooth muscle and eccrine glands: unusual histopathologic features in a familial setting

Borroni, Riccardo G.; Grassi, Sara; Concardi, Monica; Puccio, Ignazio; Giordano, Calogero; Agozzino, Manuela; Caspani, Clelia; Grasso, Maurizia; Diegoli, Marta; Arbustini, Eloisa - 01i Case report

rivista: JOURNAL OF CUTANEOUS PATHOLOGY (Blackwell Munksgaard:PO Box 2148, Periodicals Department, DK-1016 Copenhagen K Denmark:011 45 33 755913, EMAIL: agentservices@oxon.blackwellpublishing.com, INTERNET: http://www.blackwellmunksgaard.com, Fax: 011 45 77 333377) pp. 308-315 - issn: 0303-6987 - wos: WOS:000331606000007 (7) - scopus: 2-s2.0-84894561892 (9)

11573/1109806 - 2014 - Incomplete penetrance of GLMN gene c.395-1G>C mutation in a family with glomuvenous malformations

Borroni, Riccardo G.; Grassi, Sara; Diegoli, Marta; Grasso, Maurizia; Arbustini, Eloisa - 01a Articolo in rivista

rivista: INTERNATIONAL JOURNAL OF DERMATOLOGY (Blackwell Science Limited:PO Box 88, Oxford OX2 0NE United Kingdom:011 44 1865 776868, 011 44 1865 206038, EMAIL: journals.cs@blacksci.co.uk, INTERNET: http://www.blackwell-science.com, Fax: 011 44 1865 721205) pp. 1362-1364 - issn: 0011-9059 - wos: WOS:000344177600029 (1) - scopus: 2-s2.0-84911973192 (2)

11573/1109808 - 2014 - Pompholyx of the hands after intravenous immunoglobulin therapy for clinically isolated syndrome: A paediatric case

Brazzelli, Valeria; Grassi, S.; Savasta, S.; Ruffinazzi, G.; Carugno, A.; Barbaccia, V.; Marseglia, G. L.; Borroni, G. - 01a Articolo in rivista

rivista: INTERNATIONAL JOURNAL OF IMMUNOPATHOLOGY AND PHARMACOLOGY (- London : SAGE Publications Ltd - Chieti : Biomedical Research Press, 1988-) pp. 127-130 - issn: 0394-6320 - wos: WOS:000334647900017 (6) - scopus: 2-s2.0-84900551763 (7)

11573/1109819 - 2013 - Panniculitis in children

Grassi, S; Borroni, R G; Brazzelli, V - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino: Minerva medica) pp. 371-385 - issn: 0392-0488 - wos: WOS:000325664700006 (9) - scopus: 2-s2.0-84887072248 (15)

11573/1109814 - 2013 - Post-surgical lipophagic panniculitis: a specific model of traumatic panniculitis and new histopathological findings

Grassi, S; Rosso, R; Tomasini, C; Pezzini, C; Merlino, M; Borroni, G - 01a Articolo in rivista

rivista: GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA (Torino: Minerva medica) pp. 435-441 - issn: 0392-0488 - wos: WOS:000325664700012 (0) - scopus: 2-s2.0-84887043339 (2)