CATERINA SANCHINI

Dottoressa di ricerca

ciclo: XXXV


supervisore: Maria Rosito
relatore: Silvia Di Angelantonio
co-supervisore: Silvia Di Angelatonio

Titolo della tesi: Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays

Microglia reactivity entails a large-scale remodeling of cellular geometry, but while the role of the actin cytoskeleton in driving these morphological changes and the specialized functions of activated microglia has been extensively studied, the behavior of the microtubule cytoskeleton during these changes remains unexplored. We hypothesize that rearrangement of the microtubule cytoskeleton would play a major role in the morphological changes guiding microglia transition from homeostatic to activated states providing a novel target to modulate microglia activation. Through a detailed in vitro phenotyping approach and validation in mouse retinal tissue we report that: 1) in homeostatic conditions, ramified microglia possess stable microtubule arrays, while microglia activation increases microtubule dynamic behavior. 2) A non-centrosomal microtubule organization in arrays with mixed polarity is a hallmark of homeostatic microglia, similar to the architecture typical of highly specialized cells such as neurons and oligodendrocytes, while activation induces recruitment of the majority of microtubule minus end at the centrosome. 3) Homeostatic microglia nucleate non-centrosomal microtubules at Golgi outposts, similar to what occurs in neurons, while activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results unveil the remodeling of the microtubule cytoskeleton as a striking hallmark of microglia reactivity and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of activated microglia, inhibition of microtubule dynamics may provide a novel strategy to reduce microglia reactivity in inflammatory disease.

Produzione scientifica

11573/1685183 - 2023 - Unlocking Neural Function with 3D In Vitro Models: A Technical Review of Self-Assembled, Guided, and Bioprinted Brain Organoids and Their Applications in the Study of Neurodevelopmental and Neurodegenerative Disorders
D'antoni, Chiara; Mautone, Lorenza; Sanchini, Caterina; Tondo, Lucrezia; Grassmann, Greta; Cidonio, Gianluca; Bezzi, Paola; Cordella, Federica; Di Angelantonio, Silvia - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 10762- - issn: 1422-0067 - wos: WOS:001030121200001 (1) - scopus: 2-s2.0-85165073149 (1)

11573/1669493 - 2023 - Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays
Rosito, Maria; Sanchini, Caterina; Gosti, Giorgio; Moreno, Manuela; De Panfilis, Simone; Giubettini, Maria; Debellis, Doriana; Catalano, Federico; Peruzzi, Giovanna; Marotta, Roberto; Indrieri, Alessia; De Leonibus, Elvira; De Stefano, Maria Egle; Ragozzino, Davide; Ruocco, Giancarlo; Di Angelantonio, Silvia; Bartolini, Francesca - 01a Articolo in rivista
rivista: CELL REPORTS (Elsevier B.V. Cambridge MA: Cell Press) pp. - - issn: 2211-1247 - wos: WOS:000965814900001 (5) - scopus: 2-s2.0-85147959873 (3)

11573/1686280 - 2023 - Protocol for observing microtubules and microtubule ends in both fixed and live primary microglia cells
Sanchini, Caterina; Rosito, Maria; Comincini, Alessandro; De Panfilis, Simone; Bartolini, Francesca; Di Angelantonio, Silvia - 01a Articolo in rivista
rivista: STAR PROTOCOLS (: New York: Cell Press Elsevier Inc) pp. 102499- - issn: 2666-1667 - wos: WOS:001062211600001 (0) - scopus: 2-s2.0-85167594990 (0)

11573/1660557 - 2022 - Substrate stiffness effect on molecular crosstalk of epithelial-mesenchymal transition mediators of human glioblastoma cells
Basilico, Bernadette; Palamà, Ilaria Elena; D'amone, Stefania; Lauro, Clotilde; Rosito, Maria; Grieco, Maddalena; Ratano, Patrizia; Cordella, Federica; Sanchini, Caterina; Di Angelantonio, Silvia; Ragozzino, Davide; Cascione, Mariafrancesca; Gigli, Giuseppe; Cortese, Barbara - 01a Articolo in rivista
rivista: FRONTIERS IN ONCOLOGY (Lausanne : Frontiers Editorial Office, 2011-) pp. - - issn: 2234-943X - wos: WOS:000856524900001 (2) - scopus: 2-s2.0-85137975276 (2)

11573/1582800 - 2021 - Microglia control glutamatergic synapses in the adult mouse hippocampus
Basilico, B.; Ferrucci, L.; Ratano, P.; Golia, M. T.; Grimaldi, A.; Rosito, M.; Ferretti, V.; Reverte, I.; Sanchini, C.; Marrone, M. C.; Giubettini, M.; De Turris, V.; Salerno, D.; Garofalo, S.; St-Pierre, M. -K.; Carrier, M.; Renzi, M.; Pagani, F.; Modi, B.; Raspa, M.; Scavizzi, F.; Gross, C. T.; Marinelli, S.; Tremblay, M. -E.; Caprioli, D.; Maggi, L.; Limatola, C.; Di Angelantonio, S.; Ragozzino, D. - 01a Articolo in rivista
rivista: GLIA (John Wiley & Sons Incorporated:Customer Service, 111 River Street:Hoboken, NJ 07030:(800)225-5945, (201)748-6000, EMAIL: societyinfo@wiley.com, INTERNET: http://www.wiley.com, Fax: (212)748-6551) pp. - - issn: 0894-1491 - wos: WOS:000708025800001 (29) - scopus: 2-s2.0-85116258492 (33)

11573/1582798 - 2021 - Antibiotics treatment modulates microglia–synapses interaction
Cordella, Federica; Sanchini, Caterina; Rosito, Maria; Ferrucci, Laura; Pediconi, Natalia; Cortese, Barbara; Guerrieri, Francesca; Pascucci, Giuseppe Rubens; Antonangeli, Fabrizio; Peruzzi, Giovanna; Giubettini, Maria; Basilico, Bernadette; Pagani, Francesca; Grimaldi, Alfonso; D’Alessandro, Giuseppina; Limatola, Cristina; Ragozzino, Davide; Di Angelantonio, Silvia - 01a Articolo in rivista
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. - - issn: 2073-4409 - wos: WOS:000717121200001 (13) - scopus: 2-s2.0-85116280965 (13)

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