ROSSELLA TOZZI

Dottoressa di ricerca

ciclo: XXXV


supervisore: Prof.ssa Stefania Mariani
relatore: Prof. Lucio Gnessi

Titolo della tesi: Role of SIRT1 in the regulation of adipose tissue and metabolism: implications with the ketogenic diet.

Sirtuin1 (SIRT1) has a regulatory role mainly in metabolism and aging, extending health span through effects able to prevent metabolic derangements and degenerative diseases, including aging-related bone loss. Being a histone deacetylase using NAD as a cofactor, its activity increases with caloric restriction or starvation. Overnutrition reduce SIRT1, while transgenic mice for SIRT1 overexpression are protected from fatty liver, type 2 diabetes (T2D), age- induced cancer and osteoporosis. We demonstrated that circulating SIRT1 – as well as the tissue – is inversely correlated with fat amounts and coherently associated with the levels of the two well-known adipokines adiponectin and leptin, being predictive of visceral adipose tissue (VAT), specifically. In addition, circulating SIRT1 proved to be directly correlated with bone mineral density and was the best predictor of trabecular structure, reflecting the negative relationship with sclerostin – an osteoblast inhibitor – observed in murine studies. Ketogenesis is a metabolic process involved in controlling energy homeostasis by a fine epigenetic regulation implemented during fasting or intense exercise. Endogenous or exogenous ketosis delay aging by improving anti-inflammatory and mitochondrial activities, mnemonic and muscle performance and reduces metabolic diseases through the disposal of fatty acids. Being proposed as an alternative therapy option even during acute illness such as COVID-19, the ketogenic diet (KD) has been successfully introduced for the treatment of obesity and its main complications such as NAFLD and T2D. Recent advances in understanding the influence on health span and the shared outcomes between ketone bodies and SIRT1 suggest a joint work with the same beneficial intent, probably also via epigenetic modulation. The aim of our study was to understand whether a KD leads to the increase of SIRT1, assuming the role of SIRT1 as a downstream effector of the metabolic adaptations observed during ketosis, beyond caloric restriction and weight loss. We evaluated 20 C57BL/6J 8 weeks old male mice treated with isocaloric KD or standard diet of the same caloric intake for 4 weeks. Blood and urine were collected at baseline and after the sacrifice. SIRT1 was evaluated in serum, liver, and adipose tissue including VAT, subcutaneous adipose tissue (SAT) and brown adipose tissue (BAT). All organs were weighed dry and collected as follows: 1/2 in 10% Neutral Buffered Formalin Solution (for histology) and 1/2 dry at -80°C (for molecular analyses). After 4 weeks, despite the 1.7-fold higher caloric intake, KD-fed mice showed no significative changes in weight gain, VAT (g), SAT (g), glycemia (mg/dl) and TRG (mg/dl). Ketosis was efficaciously achieved in the treated group (p<0.001) and serum SIRT1 increased significantly compared to the controls under normal chow (p<0.05). Tissue SIRT1 expression increased both in VAT and SAT in mice under KD compared to normal chow group (p<0.05), while no differences were seen regarding liver and BAT. This study supports the hypothesis of a ketone body-mediated activation of SIRT1, specifically exerted in white adipose tissue, able to counteract the development of metabolic derangements. The possible cooperation between ketone bodies and SIRT1 could be of considerable interest in terms of promoting metabolic health.

Produzione scientifica

  • 11573/1687795 - 2023 - Pituitary T1 signal intensity at magnetic resonance imaging is reduced in patients with obesity: results from the CHIASM study (01a Articolo in rivista)
    PULIANI, GIULIA; SBARDELLA, EMILIA; COZZOLINO, ALESSIA; SADA, VALENTINA; TOZZI, ROSSELLA; ANDREOLI, CHIARA; FIORELLI, MARCO; DI BIASI, CLAUDIO; CORALLINO, DILETTA; BALLA, ANDREA; PAGANINI, ALESSANDRO MARIA; VENNERI, MARY ANNA; LENZI, ANDREA; LUBRANO, CARLA; ISIDORI, ANDREA
  • 11573/1250541 - 2018 - Bmd/bmi ratio and trabecular bone score: new potential tools to assess the risk of fracture in obese patients (04d Abstract in atti di convegno)
    WATANABE, MIKIKO; COSTANTINI, DANIELA; TOZZI, ROSSELLA; DE GIORGI, ROBERTA; GANGITANO, ELENA; POGGIOGALLE, ELEONORA; MARIANI, STEFANIA; BASCIANI, SABRINA; PETRANGELI, ELISA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1250536 - 2018 - Secrezione di GH e morfologia ipofisaria in pazienti obesi (04d Abstract in atti di convegno)
    TOZZI, ROSSELLA; WATANABE, MIKIKO; DE GIORGI, FRANCESCA; COSTANTINI, DANIELA; CAPUTI, ALESSANDRA; VARI, MARIA; CONTINI, SAVINA; PETRANGELI, ELISA; MARIANI, STEFANIA; ISIDORI, ANDREA; LUBRANO, CARLA
  • 11573/1250530 - 2018 - Impatto dei farmaci modulatori della proteina CFTR sulle alterazioni dell’asse GH – IGF-1 in pazienti adulti affetti da Fibrosi Cistica (04d Abstract in atti di convegno)
    DE BIASE, RICCARDO VALERIO; WATANABE, MIKIKO; TOZZI, ROSSELLA; GANGITANO, ELENA; DI GIORGIO, MARIA ROSARIA; VARI, MARIA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1622184 - 2022 - Circulating SIRT1 and Sclerostin Correlates with Bone Status in Young Women with Different Degrees of Adiposity (01a Articolo in rivista)
    TOZZI, ROSSELLA; MASI, DAVIDE; CIPRIANI, FIAMMETTA; CONTINI, SAVINA; GANGITANO, ELENA; SPOLTORE, MARIA ELENA; BARCHETTA, ILARIA; BASCIANI, SABRINA; WATANABE, MIKIKO; BALDINI, ENKE; ULISSE, SALVATORE; LUBRANO, CARLA; GNESSI, LUCIO; MARIANI, STEFANIA
  • 11573/1381535 - 2020 - Beneficial effects of the ketogenic diet on nonalcoholic fatty liver disease: A comprehensive review of the literature (01g Articolo di rassegna (Review))
    WATANABE, MIKIKO; TOZZI, ROSSELLA; RISI, RENATA; MARIANI, STEFANIA; BASCIANI, SABRINA; SPERA, GIOVANNI; LUBRANO, CARLA; GNESSI, LUCIO
  • 11573/1687796 - 2023 - Correction: Pituitary T1 signal intensity at magnetic resonance imaging is reduced in patients with obesity: results from the CHIASM study (01b Commento, Erratum, Replica e simili)
    PULIANI, GIULIA; SBARDELLA, EMILIA; COZZOLINO, ALESSIA; SADA, VALENTINA; TOZZI, ROSSELLA; FIORELLI, MARCO; DI BIASI, CLAUDIO; CORALLINO, DILETTA; BALLA, ANDREA; PAGANINI, ALESSANDRO MARIA; VENNERI, MARY ANNA; LENZI, ANDREA; LUBRANO, CARLA; ISIDORI, ANDREA
  • 11573/1136601 - 2018 - Overweight and obese patients with nickel allergy have a worse metabolic profile compared to weight matched non-allergic individuals (01a Articolo in rivista)
    WATANABE, MIKIKO; MASIERI, SIMONETTA; TOZZI, ROSSELLA; GANGITANO, ELENA; POGGIOGALLE, ELEONORA; MARIANI, STEFANIA; BASCIANI, SABRINA; PETRANGELI, ELISA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1250520 - 2017 - Metabolically Healthy Obesity: an Endocrinological Issue? (04d Abstract in atti di convegno)
    WATANABE, MIKIKO; POGGIOGALLE, ELEONORA; TOZZI, ROSSELLA; BASCIANI, SABRINA; MARIANI, STEFANIA; PETRANGELI, ELISA; DONINI, LORENZO MARIA; LENZI, ANDREA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1250527 - 2017 - Sensibilità al nickel in una popolazione italiana sovrappeso ed obesa (04d Abstract in atti di convegno)
    WATANABE, MIKIKO; PETRANGELI, ELISA; MARIANI, STEFANIA; TOZZI, ROSSELLA; VARI, MARIA; GANGITANO, ELENA; LENZI, ANDREA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1463028 - 2020 - Blood sirt1 shows a coherent association with leptin and adiponectin in relation to the degree and distribution of adiposity: A study in obesity, normal weight and anorexia nervosa (01a Articolo in rivista)
    MARIANI, STEFANIA; DI GIORGIO, MARIA ROSARIA; ROSSI, ERICA; TOZZI, ROSSELLA; CONTINI, SAVINA; CIPRIANI, FIAMMETTA; TOSCANO, RAFFAELLA; BASCIANI, SABRINA; BARBARO, GIUSEPPE; WATANABE, MIKIKO; LUBRANO, CARLA; GNESSI, LUCIO
  • 11573/1474647 - 2021 - Case report: pituitary morphology and function are preserved in female patients with idiopathic intracranial hypertension under pharmacological treatment (01i Case report)
    TOZZI, ROSSELLA; MORAMARCO, ANTONIETTA; WATANABE, MIKIKO; BALENA, ANGELA; CAPUTI, ALESSANDRA; GANGITANO, ELENA; PETRANGELI, ELISA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1540141 - 2021 - Ketogenic diet as a preventive and supportive care for covid-19 patients (01g Articolo di rassegna (Review))
    GANGITANO, ELENA; TOZZI, ROSSELLA; GANDINI, ORIETTA; WATANABE, MIKIKO; BASCIANI, SABRINA; MARIANI, STEFANIA; LENZI, ANDREA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1499862 - 2019 - Oxytocin and emotional dysregulation in obese patients: a pilot study (04d Abstract in atti di convegno)
    GANGITANO, ELENA; TOZZI, ROSSELLA; WATANABE, MIKIKO; BASCIANI, SABRINA; CAPUTI, ALESSANDRA; COSTANTINI, DANIELA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1499819 - 2020 - La capacità secretoria di GH costituisce un importante determinante del fenotipo metabolico nei pazienti sovrappeso ed obesi (04f Poster)
    GANGITANO, ELENA; CAPUTI, ALESSANDRA; TOZZI, ROSSELLA; WATANABE, MIKIKO; BALENA, ANGELA; RISI, RENATA; MASI, DAVIDE; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560429 - 2021 - Sirt1 and sclerostin: a pathophysiological connection between obesity and bone (04d Abstract in atti di convegno)
    TOZZI, ROSSELLA; SPOLTORE, MARIA ELENA; MASI, DAVIDE; CONTINI, SAVINA; WATANABE, MIKIKO; LUBRANO, CARLA; GNESSI, LUCIO; MARIANI, STEFANIA
  • 11573/1560446 - 2017 - Osteoprotegerin levels are related to endothelial dysfunction in obese women (04f Poster)
    TOZZI, ROSSELLA; BERTONE, CORA; GANGITANO, ELENA; BASCIANI, SABRINA; MARIANI, STEFANIA; LENZI, ANDREA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560423 - 2017 - Obesity, cardiomyopathy and anabolic hormones (01h Abstract in rivista)
    GANGITANO, ELENA; COSTANTINI, DANIELA; TOZZI, ROSSELLA; POGGIOGALLE, ELEONORA; MARIANI, STEFANIA; PETRANGELI, ELISA; DONINI, LORENZO MARIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560428 - 2021 - Evaluation of plasma levels of oxytocin in relation to body composition and cardiovascular risk parameters in obese and normal weight subjects. (04d Abstract in atti di convegno)
    TOZZI, ROSSELLA; ROSSETTI, REBECCA; WATANABE, MIKIKO; GANGITANO, ELENA; GNESSI, LUCIO; MARIANI, STEFANIA; LUBRANO, CARLA
  • 11573/1558060 - 2021 - Beyond weight loss in nonalcoholic fatty liver disease: the role of carbohydrate restriction (01g Articolo di rassegna (Review))
    RISI, RENATA; TOZZI, ROSSELLA; WATANABE, MIKIKO
  • 11573/1560483 - 2019 - Binge eating disorder is associated with lower glucose levels three hours after an oral glucose tolerance test (01h Abstract in rivista)
    WATANABE, MIKIKO; TOZZI, ROSSELLA; CAPUTI, ALESSANDRA; POGGIOGALLE, ELEONORA; RISI, RENATA; BALENA, ANGELA; GANGITANO, ELENA; BASCIANI, SABRINA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560515 - 2021 - Chondrosarcoma of the cavernous sinus treated with postoperative proton radiation therapy: case report and endocrinological follow up at 24 months (04d Abstract in atti di convegno)
    MASI, DAVIDE; RISI, RENATA; BALENA, ANGELA; CAPUTI, ALESSANDRA; SPOLTORE, MARIA ELENA; ROSSETTI, REBECCA; WATANABE, MIKIKO; TOZZI, ROSSELLA; GANGITANO, ELENA; MARIANI, STEFANIA; LUBRANO, CARLA; GNESSI, LUCIO
  • 11573/1560496 - 2020 - Body composition, oxidative stress and bone strength parameters in patients with obesity: a cross sectional observational study (04d Abstract in atti di convegno)
    WATANABE, MIKIKO; TOZZI, ROSSELLA; GANGITANO, ELENA; CAPUTI, ALESSANDRA; RISI, RENATA; BALENA, ANGELA; MASI, DAVIDE; MARIANI, STEFANIA; BASCIANI, SABRINA; PETRANGELI, ELISA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1555926 - 2021 - Central obesity, smoking habit, and hypertension are associated with lower antibody titres in response to COVID‐19 mRNA vaccine (01a Articolo in rivista)
    WATANABE, MIKIKO; BALENA, ANGELA; TOZZI, ROSSELLA; RISI, RENATA; MASI, DAVIDE; CAPUTI, ALESSANDRA; ROSSETTI, REBECCA; SPOLTORE, MARIA ELENA; FILIPPI, VALERIA; GANGITANO, ELENA; MARIANI, STEFANIA; LUBRANO, CARLA; LENZI, ANDREA; MASTROIANNI, CLAUDIO MARIA; GNESSI, LUCIO
  • 11573/1560431 - 2019 - Oxytocin levels correlate with metabolic syndrome in obese patients (04f Poster)
    TOZZI, ROSSELLA; GANGITANO, ELENA; WATANABE, MIKIKO; BASCIANI, SABRINA; CAPUTI, ALESSANDRA; COSTANTINI, DANIELA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1607458 - 2022 - Rapid Weight Loss, Central Obesity Improvement and Blood Glucose Reduction Are Associated with a Stronger Adaptive Immune Response Following COVID-19 mRNA Vaccine (01a Articolo in rivista)
    WATANABE, MIKIKO; BALENA, ANGELA; MASI, DAVIDE; TOZZI, ROSSELLA; RISI, RENATA; CAPUTI, ALESSANDRA; ROSSETTI, REBECCA; SPOLTORE, MARIA ELENA; ANASTASI, EMANUELA; ANGELONI, ANTONIO; MARIANI, STEFANIA; LUBRANO, CARLA; GNESSI, LUCIO
  • 11573/1629451 - 2022 - The Influence of Ketone Bodies on Circadian Processes Regarding Appetite, Sleep and Hormone Release: A Systematic Review of the Literature (01a Articolo in rivista)
    MASI, DAVIDE; SPOLTORE, MARIA ELENA; ROSSETTI, REBECCA; WATANABE, MIKIKO; TOZZI, ROSSELLA; CAPUTI, ALESSANDRA; RISI, RENATA; BALENA, ANGELA; GANDINI, ORIETTA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1613900 - 2021 - Ketogenic diet for obese COVID-19 patients: is respiratory disease a contraindication? A narrative review of the literature on ketogenic diet and respiratory function (01g Articolo di rassegna (Review))
    GANGITANO, ELENA; TOZZI, ROSSELLA; MARIANI, STEFANIA; LENZI, ANDREA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1645811 - 2022 - Reduction in Oxytocin levels predict body weight loss in patients with obesity after a very low carbohydrate ketogenic diet (VLCKD). (01h Abstract in rivista)
    TOZZI, ROSSELLA; GANGITANO, ELENA; WATANABE, MIKIKO; MARIANI, STEFANIA
  • 11573/1654699 - 2022 - Ketone Bodies and SIRT1, Synergic Epigenetic Regulators for Metabolic Health: A Narrative Review (01g Articolo di rassegna (Review))
    TOZZI, ROSSELLA; CIPRIANI, FIAMMETTA; MASI, DAVIDE; BASCIANI, SABRINA; WATANABE, MIKIKO; LUBRANO, CARLA; GNESSI, LUCIO; MARIANI, STEFANIA
  • 11573/1643037 - 2022 - Sex Difference in the Safety and Efficacy of Bariatric Procedures: a Systematic Review and Meta-analysis (01g Articolo di rassegna (Review))
    RISI, RENATA; TOZZI, ROSSELLA; MASI, DAVIDE; CASTAGNETO GISSEY, LIDIA; CASELLA, GIOVANNI; GNESSI, LUCIO; WATANABE, MIKIKO
  • 11573/1664774 - 2022 - Growth Hormone Secretory Capacity Is Associated with Cardiac Morphology and Function in Overweight and Obese Patients: A Controlled, Cross-Sectional Study (01a Articolo in rivista)
    GANGITANO, ELENA; ROSSETTI, REBECCA; SPOLTORE, MARIA ELENA; MASI, DAVIDE; TOZZI, ROSSELLA; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1664775 - 2022 - Oxytocin correlates with body dysmorphic concerns and predicts weight loss in patients with obesity after a very low carbohydrate ketogenic diet (01h Abstract in rivista)
    TOZZI, ROSSELLA; ROSSETTI, REBECCA; BASCIANI, SABRINA; MASI, DAVIDE; RISI, RENATA; BALENA, ANGELA; SPOLTORE, MARIA ELENA; WATANABE, MIKIKO; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1664670 - 2023 - Genome Editing and Obesity (02a Capitolo o Articolo)
    MASI, DAVIDE; TOZZI, ROSSELLA; WATANABE, MIKIKO
  • 11573/1662449 - 2022 - Ketogenic Diet Increases Serum and White Adipose Tissue SIRT1 Expression in Mice (01a Articolo in rivista)
    TOZZI, ROSSELLA; CAMPOLO, FEDERICA; BALDINI, ENKE; VENNERI, MARY ANNA; LUBRANO, CARLA; ULISSE, SALVATORE; GNESSI, LUCIO; MARIANI, STEFANIA
  • 11573/1379722 - 2020 - Very Low-Calorie Ketogenic Diet: a safe and effective tool for weight loss in patients with obesity and mild kidney failure (01a Articolo in rivista)
    TOZZI, ROSSELLA; BALENA, ANGELA; MARIANI, STEFANIA; BASCIANI, SABRINA; SPERA, GIOVANNI; GNESSI, LUCIO; LUBRANO, CARLA; WATANABE, MIKIKO
  • 11573/1469793 - 2020 - Nickel sensitivity is associated with GH-IGF1 axis impairment and pituitary abnormalities on MRI in overweight and obese subjects (01a Articolo in rivista)
    RISI, RENATA; MASIERI, SIMONETTA; POGGIOGALLE, ELEONORA; WATANABE, MIKIKO; CAPUTI, ALESSANDRA; TOZZI, ROSSELLA; GANGITANO, ELENA; MASI, DAVIDE; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560506 - 2021 - GROWTH HORMONE SECRETORY CAPACITY AND BONE HEALTH INDICES IN OVERWEIGHT AND OBESE FEMALE PATIENTS (04d Abstract in atti di convegno)
    GANGITANO, ELENA; CAPUTI, ALESSANDRA; BALENA, ANGELA; ROSSETTI, REBECCA; SPOLTORE, MARIA ELENA; TOZZI, ROSSELLA; MASI, DAVIDE; RISI, RENATA; WATANABE, MIKIKO; MARIANI, STEFANIA; GNESSI, LUCIO; LUBRANO, CARLA
  • 11573/1560503 - 2020 - Circulating lymphocytes characterization in obese subjects: a correlation with risk factors for cancer (04d Abstract in atti di convegno)
    WATANABE, MIKIKO; TOZZI, ROSSELLA; GANGITANO, ELENA; CAPUTI, ALESSANDRA; RISI, RENATA; BALENA, ANGELA; MASI, DAVIDE; MARIANI, STEFANIA; BASCIANI, SABRINA; GNESSI, LUCIO; LUBRANO, CARLA

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