ROBERTA PIOVESANA

Dottoressa di ricerca

ciclo: XXXII


relatore: Ada Maria Tata

Titolo della tesi: Expression and functional characterization of Acetylcholine M2 muscarinic receptor in rat and human Schwann-like adipose-derived stem cells

Schwann cells (SCs) play a central role in peripheral nervous system physiology and in the response to axon injury. However, there are several restrictions hindering their clinical application, such as the difficulty in collection and a slow in vitro expansion. Adipose-derived stem cells (ASCs) present good properties for peripheral nerve regeneration. When exposed to specific growth factors in vitro, they can acquire a SC-like phenotype (dASCs) expressing key SCs markers and assuming spindle-shaped morphology. Cholinergic receptors are potential pharmacological targets and in particular, M2 subtype, are present in SCs and are able to modulate several physiological processes. In the current work the role of M2 muscarinic receptor activation in controlling rat and human dASCs proliferation, neurotrophic factor production and differentiation was extensively evaluated. ASCs differentiation protocol is a chemical stimulation and after growth factors withdrawal, dASCs revert their morphology and gene expression towards ASCs phenotype. We show that M2 stimulation is able to maintain a spindle-like morphology and improved SCs phenotype. These data are the first evidence that human dASCs are cholinoceptive and respond in a similar way to muscarinic agonists as rat dASCs, improving dASCs terminal differentiation.

Produzione scientifica

11573/1615240 - 2022 - Analysis of Signal Transduction Pathways Downstream M2 Receptor Activation: Effects on Schwann Cell Migration and Morphology
Botticelli, Elisabetta; Salazar Intriago, Michael Sebastian; Piovesana, Roberta; Tata, Ada Maria - 01a Articolo in rivista
rivista: LIFE (Basel: MDPI) pp. - - issn: 2075-1729 - wos: WOS:000763790400001 (4) - scopus: 2-s2.0-85124479180 (4)

11573/1615238 - 2022 - Notch signal mediates the cross-interaction between M2 muscarinic acetylcholine receptor and neuregulin/ErbB pathway: effects on schwann cell proliferation
Piovesana, Roberta; Pisano, Annalinda; Loreti, Simona; Ricordy, Ruggero; Talora, Claudio; Tata, Ada Maria - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. - - issn: 2218-273X - wos: WOS:000767579600001 (2) - scopus: 2-s2.0-85123734775 (2)

11573/1701373 - 2022 - Emerging Roles of Cholinergic Receptors in Schwann Cell Development and Plasticity
Piovesana, Roberta; Reid, Adam J; Tata, Ada Maria - 01g Articolo di rassegna (Review)
rivista: BIOMEDICINES (Basel : MDPI) pp. 1-15 - issn: 2227-9059 - wos: WOS:000916835300001 (1) - scopus: 2-s2.0-85146727319 (1)

11573/1603353 - 2021 - The mechanisms mediated by α7 acetylcholine nicotinic receptors may contribute to peripheral nerve regeneration
Intriago, M. S. S.; Piovesana, R.; Matera, A.; Taggi, M.; Canipari, R.; Fabrizi, C.; Papotto, C.; Matera, C.; De Amici, M.; Dallanoce, C.; Tata, A. M. - 01a Articolo in rivista
rivista: MOLECULES (Basel: MDPI Berlin: Springer, 1996-) pp. 1-13 - issn: 1420-3049 - wos: WOS:000737031800001 (5) - scopus: 2-s2.0-85121504338 (5)

11573/1550222 - 2021 - Cholinergic modulation of neuroinflammation: Focus on α7 nicotinic receptor
Piovesana, R.; Intriago, M. S. S.; Dini, L.; Tata, A. M. - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel (Matthaeustrasse 11) : Molecular Diversity Preservation International MDPI) pp. 1-12 - issn: 1661-6596 - wos: WOS:000650441400001 (36) - scopus: 2-s2.0-85105186433 (35)

11573/1458395 - 2020 - Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells
Pernarella, M.; Piovesana, R.; Matera, C.; Faroni, A.; Fiore, M.; Dini, L.; Reid, A. J.; Dallanoce, C.; Tata, A. M. - 01a Articolo in rivista
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Tipolitografia l Ponzio:Viale Montegrappa 2, I 27100 Pavia Italy:011 39 0382 576600, Fax: 011 39 0382 574200) pp. 61-70 - issn: 1121-760X - wos: WOS:000614541300008 (6) - scopus: 2-s2.0-85095729908 (5)

11573/1390819 - 2020 - Muscarinic receptors modulate Nerve Growth Factor production in rat Schwann-like adipose-derived stem cells and in Schwann cells
Piovesana, R; Faroni, A; Taggi, M; Matera, A; Soligo, M; Canipari, R; Manni, L; Reid, A J; Tata, A M - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. 7159-7174 - issn: 2045-2322 - wos: WOS:000560713100010 (18) - scopus: 2-s2.0-85084007363 (19)

11573/1440084 - 2020 - Functional Characterization of Muscarinic Receptors in Human Schwann Cells
Piovesana, Roberta; Faroni, Alessandro; Tata, Ada Maria; Reid, Adam J - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. -1 - issn: 1422-0067 - wos: WOS:000580745300001 (8) - scopus: 2-s2.0-85090659136 (8)

11573/1465043 - 2020 - Schwann-like adipose-derived stem cells as a promising therapeutic tool for peripheral nerve regeneration: effects of cholinergic stimulation
Piovesana, Roberta; Faroni, Alessandro; Tata, Ada Maria; Reid, Adam J - 01a Articolo in rivista
rivista: NEURAL REGENERATION RESEARCH (Shenyang : Publishing House of Neural Regeneration Research.) pp. 1218-1220 - issn: 1673-5374 - wos: WOS:000598623300042 (10) - scopus: 2-s2.0-85097398921 (10)

11573/1272600 - 2019 - M2 receptors activation modulates cell growth, migration and differentiation of rat Schwann-like adipose-derived stem cells
Piovesana, Roberta; Faroni, Alessandro; Magnaghi, Valerio; Reid, Adam J; Tata, Ada Maria - 01a Articolo in rivista
rivista: CELL DEATH DISCOVERY ([London]: Nature Publishing Group, 2015-) pp. - - issn: 2058-7716 - wos: WOS:000467507100001 (13) - scopus: 2-s2.0-85070453414 (14)

11573/1113052 - 2018 - Mir-34a-5p Mediates Cross-Talk between M2 Muscarinic Receptors and Notch-1/EGFR Pathways in U87MG Glioblastoma Cells: Implication in Cell Proliferation
Di Bari, Maria; Bevilacqua, Valeria; De Jaco, Antonella; Laneve, Pietro; Piovesana, Roberta; Trobiani, Laura; Talora, Claudio; Caffarelli, Elisa; Tata, Ada Maria - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 1631- - issn: 1422-0067 - wos: WOS:000436506600086 (24) - scopus: 2-s2.0-85047958073 (24)

11573/1174771 - 2018 - Expression of acetylcholine nicotinic receptor a7 in rat Schwann cells: implication in nerve regeneration
Matera, Alessandro; Piovesana, R.; Taggi, M.; Canipari, R.; Dallanoce, C.; Fabrizi, C.; Tata, A. M. - 04f Poster
congresso: XV FISV Congress (Rome)
libro: XV FISV CONGRESS Programme and Abstracts Sapienza University of Rome, Italy September 18 – 21, 2018 - ()

11573/1185116 - 2018 - Muscarinic receptor in Schwann-like adipose derived stem cells: implications in nerve regeneration.
Piovesana, R.; Faroni, A.; Magnaghi, V.; Reid, A.; Tata, Am - 04f Poster
congresso: MaRMN Symposium (Manchester)
libro: MaRMN Symposium - ()

11573/1184968 - 2018 - M2 receptors activation affects proliferation, migration and neurotrophic factors production in rat Schwann-like cells.
Piovesana, R.; Faroni, A.; Matera, A.; Soligo, M.; Protto, V.; Manni, L.; Magnaghi, V.; Reid, Aj; Tata, Am - 04f Poster
congresso: FENS (Berlino)
libro: FENS congress - ()

11573/1185142 - 2018 - Cholinergic effects mediated by M2 muscarinic receptor in human Schwann-like cells induced from adipose mesenchymal stem cells
Piovesana, R.; Faroni, A.; Tata, Am; Reid, A. - 04f Poster
congresso: MSCA Workshop and Annual Meeting (Birmingham)
libro: MSCA Workshop and annual Meeting - ()

11573/1184868 - 2018 - Cholinergic receptors contribute to maintain the quiescent status of adipose mesenchymal stem cells
Piovesana, R; Matera, A; Pernarella, M; Magnaghi, V; Dallanoce, C; Tata, A. M. - 04d Abstract in atti di convegno
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Tipolitografia l Ponzio:Viale Montegrappa 2, I 27100 Pavia Italy:011 39 0382 576600, Fax: 011 39 0382 574200) pp. 29-29 - issn: 1121-760X - wos: (0) - scopus: (0)
congresso: GEI Gruppo embriologico italiano (L'Aquila)
libro: European Journal of Histochemistry - ()

11573/1184922 - 2018 - Cholinergic effects mediated by M2 muscarinic receptor in human Schwann-like cells induced from adipose mesenchymal stem cells
Piovesana, Roberta; Faroni, A.; Tata, Ada Maria; Reid, A. - 04f Poster
congresso: FISV congress (Roma)
libro: FISV congress - ()

11573/1070314 - 2018 - M2 muscarinic receptor activation inhibits cell proliferation and migration of rat adipose-mesenchymal stem cells
Piovesana, Roberta; Melfi, Simona; Fiore, Mario; Magnaghi, Valerio; Tata, Ada Maria - 01a Articolo in rivista
rivista: JOURNAL OF CELLULAR PHYSIOLOGY (John Wiley & Sons Incorporated:Customer Service, 111 River Street:Hoboken, NJ 07030:(800)225-5945, (201)748-6000, EMAIL: societyinfo@wiley.com, INTERNET: http://www.wiley.com, Fax: (212)748-6551) pp. 5348-5360 - issn: 0021-9541 - wos: WOS:000428849600023 (17) - scopus: 2-s2.0-85040791814 (18)

11573/1185063 - 2017 - Anti-proliferative and anti-migratory effects are mediated by M2 muscarinic receptor in Schwann-like cells induced from adipose mesenchymal stem cells: implication in nerve regeneration.
Piovesana, R.; Faroni, A.; Magnaghi, V.; Reid, A.; Tata, Am - 04f Poster
congresso: GLIA congress (Edimburgo)
libro: GLIA Congress Supplement to Glia GLIA 65:E103–E578 (2017) - ()

11573/1185010 - 2017 - Muscarinic receptor activation modulates neurotrophic factors production in rat Schwann-like cells derived from adipose mesenchymal stem cells.
Piovesana, R.; Faroni, A.; Soligo, M.; Manni, L.; Reid, Aj; Tata, Am. - 04d Abstract in atti di convegno
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Tipolitografia l Ponzio:Viale Montegrappa 2, I 27100 Pavia Italy:011 39 0382 576600, Fax: 011 39 0382 574200) pp. 22-23 - issn: 1121-760X - wos: (0) - scopus: (0)
congresso: GEI Gruppo Embriologico Italiano (Roma)
libro: European Journal of Histochemistry - ()

11573/1185079 - 2017 - Muscarinic receptor activation modulates proliferation and neurotrophic factors production in rat Schwann-like cells: implications in nerve regeneration.
Piovesana, R.; Faroni, A.; Soligo, M.; Matera, A.; Manni, L.; Magnaghi, V.; Reid, Aj; Tata, Am - 04f Poster
congresso: Sins Congress (Ischia)
libro: Sins Congress - ()

11573/963080 - 2016 - Anti-proliferative and anti-migratory effects mediated by M2 muscarinic receptors in Schwann-like cells induced from adipose mesenchymal stem cells: implication in nerve regeneration.
Piovesana, R.; Faroni, A.; Magnaghi, V.; Reid, A.; Tata, Ada Maria - 04a Atto di comunicazione a congresso
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Tipolitografia l Ponzio:Viale Montegrappa 2, I 27100 Pavia Italy:011 39 0382 576600, Fax: 011 39 0382 574200) pp. 11-12 - issn: 1121-760X - wos: (0) - scopus: (0)
congresso: GEI (Napoli)

11573/963094 - 2016 - Effects mediated by M2 muscarinic receptors activation in Schwann-like cells induced from adipose mesenchymal stem cells: implication in nerve regeneration.
Piovesana, R.; Faroni, A.; Magnaghi, V.; Reid, A.; Tata, Ada Maria - 04b Atto di convegno in volume
congresso: FISV congress (Roma)
libro: FISV - ()

11573/963104 - 2015 - M2 receptor activation controls cell growth, migration and differentiation in adipose-mesenchimal stem cells
Magnaghi, V.; Piovesana, R.; Melfi, S.; Tata, Ada Maria - 04b Atto di convegno in volume
congresso: 3rd International Symposium on Peripheral Nerve Regeneration (Hannover)
libro: 3rd International Symposium on Peripheral Nerve Regeneration - ()

11573/963108 - 2015 - Cholinergic control of cell growth and migration in undifferentiated and schwann cell–like differentiated adipose-mesenchimal stem cells.
Piovesana, Roberta; Simona, Melfi; Valerio, Magnaghi; Tata, Ada Maria - 04d Abstract in atti di convegno
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Tipolitografia l Ponzio:Viale Montegrappa 2, I 27100 Pavia Italy:011 39 0382 576600, Fax: 011 39 0382 574200) pp. 17-17 - issn: 1121-760X - wos: (0) - scopus: (0)
congresso: GEI 2015 (Pisa)
libro: European journal of histochemistry, a journal of functional cytology - ()

11573/963118 - 2015 - M2 receptor activation controls cell growth, migration and differentiation of rat adipose-mesenchimal stem cells
Piovesana, Roberta; Simona, Melfi; Valerio, Magnaghi; Tata, Ada Maria - 04b Atto di convegno in volume
congresso: GISM (Brescia)
libro: Atto di Convegno GISM - ()

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