Phd in LIFE SCIENCE

2016 –2019 Bachelor Degree: Agro-Industrial Biotechnology, "Università di Roma, La Sapienza",110/110
2018 – 2019 Erasmus project: "Universitat De Valencia"
Thesis: " Evaluation of yeast longevity K. lactis in wild and deleted mutants of gene KlMGA2"

2020 – 2022 Master Degree: Industrial, Genomics and Environmental Biotechnology,"Università di Roma, La Sapienza" 110lode/110-
06/09/2021-17/10/2022 Master Thesis Internship in the field of molecular microbiology, "Università di Roma La Sapienza", Department of Molecular Medicine. Supervisor: Alessandra Carattoli
01/03/2022 – 30/06/2022 Statens Serum Institute, Copenaghen, Denmark
Thesis:"Genomic approach for the identification of resistance mechanisms to new antibiotics and inhibitors of resistance mechanisms in Gram-negatives bacteria of clinical relevance"

2020 – 2022, Tutor of "Biology and Histology" for the course Biological Sciences, "Università di Roma, La Sapienza",

C1 IELTS certificate 2022

2016 –2019 Bachelor Degree: Agro-Industrial Biotechnology, "Università di Roma, La Sapienza",110/110
2018 – 2019 Erasmus project: "Universitat De Valencia"
Thesis: " Evaluation of yeast longevity K. lactis in wild and deleted mutants of gene KlMGA2"

2020 – 2022 Master Degree: Industrial, Genomics and Environmental Biotechnology,"Università di Roma, La Sapienza" 110lode/110-
06/09/2021-17/10/2022 Master Thesis Internship in the field of molecular microbiology, "Università di Roma La Sapienza", Department of Molecular Medicine. Supervisor: Alessandra Carattoli
01/03/2022 – 30/06/2022 Statens Serum Institute, Copenaghen, Denmark
Thesis:"Genomic approach for the identification of resistance mechanisms to new antibiotics and inhibitors of resistance mechanisms in Gram-negatives bacteria of clinical relevance"

2020 – 2022, Tutor of "Biology and Histology" for the course Biological Sciences, "Università di Roma, La Sapienza",

C1 IELTS certificate 2022

01/11/2022 – current, Roma, Italy, PhD student, University of Rome "La Sapienza"
Title of the research project: "Genomic approach for the identification of resistance mechanisms to new antibiotics and inhibitors of
resistance mechanisms in clinically relevant Gram-negative bacteria"

Thematic area: Molecular Biology, Microbiology,
SSD:Bio/19
Brief description:
This project is based on Whole Genome Sequencing (WGS) techniques, performed by rapid methods like the ONT (Oxford
Nanopore Technologies), in part complemented by Illumina sequencing. WGS is applied on key bacterial strains, with the aim
to identify endemic clones circulating in hospitals, animals, and the environment and to describe their evolution in a global
perspective. Since WGS allows the identification of microbial markers predicting and orienting empiric therapy of sepsis in
critical patients, information obtained with these studies will be used to build rapid bacterial typing methods and to follow
colonization and/or infection in critical patients. Owing to its capacity to collect multiple antimicrobial-resistance determinants, Klebsiella pneumoniae is one of the biggest threats to public health. In Italy
the dissemination of multidrug-resistant K. pneumoniae is driven by few high-risk clones expressing the KPC carbapenemase
enzyme, such as those belonging to the Clonal Groups (CGs) 101, 147, 258 and 307. I have been responsible for sequencing through the Oxford Nanopore Technologies MinION platform and Illumina platform bacteria resistant to new drugs, identifying the mechanisms
of resistance and of horizontal gene transfer

Seminars, meetings, courses:

Every two weeks attendance to Journal Club and seminars organized by the PhD school.
Participation in courses: 1) Blended Intensive Program on Molecular Scale Biophysics organized by CIVIS University alliance (12/06/2023-16/06/2023 Physical mobility in the University of Marseille, France) 2) Microscopy Techniques for the Life Sciences (12 h), 3) Preparing artworks for scientific papers (8 h online)

33rd European Congress of Clinical Microbiology & Infectious Diseases
Copenaghen , from 13th of April to 19th of April,
presenting a poster “Horizontal transfer of blaKPC plasmids mediates Ceftazidime/Avibactam resistance in Klebsiella
pneumoniae clinical isolates” R. Polani , G. Arcari, F. Sacco, F. Cecilia, G. Menichincheri, V. Capitani, G. Raponi and A. Carattoli

XXXIV° Congress SIMGBM Microbiology
Cagliari, from 21th of September to 24 September 2023 presenting a poster ” WGS based evaluation of molecular mechanisms potentially involved in reduced susceptibility to cefiderocol in
ceftazidi me/avibactam resistant Klebsiella pneumoniae clinical isolates ” R. Polani , G. Arcari, F. Sacco, G. Raponi, A. De Francesco, A. Carattoli