Pieroni Nicola

Dottore di ricerca

ciclo: XXXIV


supervisore: Alessia Cedola, Dario Coletti
relatore: Alessia Cedola
co-supervisore: Alessia Cedola

Titolo della tesi: X-Ray Phase Contrast Tomography applied to Neurodegenerative Disorders

X-ray Phase Contrast Tomography (XPTC) offers the possibility to simultaneously investigate the highly complex vascular network and neuronal network in the context of organ as a whole and, compare physiological vs. pathological states at the level of crucial disease targets. In fact, XPCT provides high-contrast and high-spatial resolution images of low absorbing tissues with no use of contrast agent or sample staining. For this reason, XPCT emerges as added-value technology for the investigation of neurodegenerative disorders. In particular, XPCT enabled us to investigate mouse model APP/PS1 of Alzheimer’s Disease (AD), demonstrating capillary occlusions and damages, close associations between plaques and damaged vessels both in the hippocampal and cerebellum regions. Moreover XPCT, changes induced in APP/PS1 mouse brains by mesenchymal stem cells exposed in vitro to AD mouse brain homogenates (MSC-CS) treatment has been investigated, unveiling MSC-CS action on amyloid plaque load. To extract quantitative information from images, a new segmentation approach has been developed based on machine learning. A random forest-based classifier has been used in order to automatically segment feature of interest in tomographic volumes including cells, vascular network and amyloid plaques. However, additional efforts are necessary for the comprehension of disease mechanisms and monitoring of therapeutic approaches. Although XPCT revealed to be a powerful technique for preclinical studies, it lacks in specificity provided by classical immunohistochemistry or histology. A combination of these techniques with XPCT has been thought and developed with the purpose of combining specificity with high-resolution 3D information. Functionalized gold nanoparticles have been injected in a mouse model of neurodegenerative disorder to target disease-relevant cell populations. The idea is to boost research in preclinical studies and shed light on future in-vivo investigation by using IH-XPCT.

Produzione scientifica

11573/1721892 - 2024 - Mesozoic larva in amber reveals the venom delivery system and the palaeobiology of an ancient lineage of venomous insects (Neuroptera)
Badano, Davide; Fratini, Michela; Palermo, Francesca; Pieroni, Nicola; Maugeri, Laura; Cerretti, Pierfilippo - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. 1-15 - issn: 2045-2322 - wos: WOS:001298603000041 (0) - scopus: 2-s2.0-85201947980 (0)

11573/1679497 - 2023 - New Cretaceous empidoids and the Mesozoic dance fly revolution (Diptera: Empidoidea)
Badano, D.; Sinclair, B. J.; Zhang, Q.; Palermo, F.; Pieroni, N.; Maugeri, L.; Fratini, M.; Cerretti, P. - 01a Articolo in rivista
rivista: CLADISTICS-THE INTERNATIONAL JOURNAL OF THE WILLI HENNIG SOCIETY (Editore indicati: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON, ENGLAND, NW1 7DX Blackwell Publishing Limited:9600 Garsington Road, Oxford OX4 2DQ United Kingdom:011 44 1865 776868 , (781)388-8200, EMAIL: agentservices@oxon.blackwellpublishing.com, e-help@blackwellpublishers.co.uk, INTERNET: http://www.blackwellpublishing.com, Fax: 011 44 1865 714591) pp. - - issn: 0748-3007 - wos: WOS:000975079300001 (2) - scopus: 2-s2.0-85153524204 (1)

11573/1552955 - 2021 - X‐ray microtomography and phylogenomics provide insights into the morphology and evolution of an enigmatic mesozoic insect larva
Badano, Davide; Fratini, Michela; Maugeri, Laura; Palermo, Francesca; Pieroni, Nicola; Cedola, Alessia; Haug, Joachim T.; Weiterschan, Thomas; Velten, Jürgen; Mei, Maurizio; Di Giulio, Andrea; Cerretti, Pierfilippo - 01a Articolo in rivista
rivista: SYSTEMATIC ENTOMOLOGY (Blackwell Science Limited:PO Box 88, Oxford OX2 0NE United Kingdom:011 44 1865 776868, 011 44 1865 206038, EMAIL: journals.cs@blacksci.co.uk, INTERNET: http://www.blackwell-science.com, Fax: 011 44 1865 721205) pp. 672-684 - issn: 0307-6970 - wos: WOS:000658714300011 (39) - scopus: 2-s2.0-85107586641 (38)

11573/1488907 - 2020 - X-ray phase contrast tomography for the investigation of amyotrophic lateral sclerosis
Ginevra Begani, Provinciali; Pieroni, N; Inna, Bukreeva; Michela, Fratini; Lorenzo, Massimi; Laura, Maugeri; Palermo, Francesca; Fabrizio, Bardelli; Alberto, Mittone; Alberto, Bravin; Giuseppe, Gigli; Francesco, Gentile; Andrea, Fossaghi; Nilo, Riva; Angelo, Quattrini; Alessia, Cedola - 01a Articolo in rivista
rivista: JOURNAL OF SYNCHROTRON RADIATION (Oxford : Blackwell Copenhagen : published for the International Union of Crystallography by Munksgaard) pp. 1042-1048 - issn: 1600-5775 - wos: WOS:000546241000021 (9) - scopus: 2-s2.0-85089541032 (9)

11573/1488892 - 2020 - Investigation of the human pineal gland 3D organization by X-ray phase contrast tomography
Inna, Bukreeva; Olga, Junemann; Alessia, Cedola; Palermo, F; Laura, Maugeri; Ginevra Begani, Provinciali; Pieroni, N; Alessia, Sanna; Dmitry A., Otlyga; Alexey, Buzmakov; Yuri, Krivonosov; Denis, Zolotov; Marina, Chukalina; Anna, Ivanova; Sergey, Saveliev; Victor, Asadchikov; Michela, Fratini - 01a Articolo in rivista
rivista: JOURNAL OF STRUCTURAL BIOLOGY (Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445) pp. 1-12 - issn: 1047-8477 - wos: WOS:000600997800022 (5) - scopus: 2-s2.0-85096211564 (6)

11573/1488888 - 2020 - Assessment of plaque morphology in alzheimer’s mouse cerebellum using three-dimensional X-ray phase-based virtual histology
Lorenzo, Massimi; Pieroni, N; Laura, Maugeri; Michela, Fratini; Francesco, Brun; Inna, Bukreeva; Giulia, Santamaria; Valentina, Medici; Tino Emanuele, Poloni; Claudia, Balducci; Alessia, Cedola - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. 1-10 - issn: 2045-2322 - wos: WOS:000548301200015 (17) - scopus: 2-s2.0-85087661327 (16)

11573/1488896 - 2020 - X-ray phase contrast tomography serves preclinical investigation of neurodegenerative diseases
Palermo, F.; Pieroni, N.; Maugeri, L.; Begani Provinciali, G.; Sanna, A.; Bukreeva, I.; Massimi, L.; Catalano, M.; Olbinado, M. P.; Fratini, M.; Uccelli, A.; Gigli, G.; Kerlero De Rosbo, N.; Balducci, C.; Cedola, A. - 01a Articolo in rivista
rivista: FRONTIERS IN NEUROSCIENCE (Lausanne : EPFL : Frontiers Research Foundation, 2007-) pp. 1-11 - issn: 1662-453X - wos: WOS:000624594800001 (0) - scopus: 2-s2.0-85096430178 (10)

11573/1488900 - 2019 - Exploring Alzheimer's disease mouse brain through X-ray phase contrast tomography: from the cell to the organ
Lorenzo, Massimi; Inna, Bukreeva; Giulia, Santamaria; Michela, Fratini; Alessandro, Corbelli; Francesco, Brun; Stefano, Fumagalli; Laura, Maugeri; Alexandra, Pacureanu; Peter, Cloetens; Pieroni, N; Fabio, Fiordaliso; Gianluigi, Forloni; Antonio, Uccelli; Nicole Kerlero De, Rosbo; Claudia, Balducci; Alessia, Cedola - 01a Articolo in rivista
rivista: NEUROIMAGE (Academic Press Incorporated:6277 Sea Harbor Drive:Orlando, FL 32887:(800)543-9534, (407)345-4100, EMAIL: ap@acad.com, INTERNET: http://www.idealibrary.com, Fax: (407)352-3445) pp. 490-495 - issn: 1053-8119 - wos: WOS:000449385000041 (50) - scopus: 2-s2.0-85053855807 (52)

11573/1488887 - 2018 - Synchrotron Radiation X-Ray Phase-Contrast Microtomography: What Opportunities More for Regenerative Medicine?
Ginevra Begani, Provinciali; Pieroni, N; Inna, Bukreeva - 02a Capitolo o Articolo
libro: Advanced High-Resolution Tomography in Regenerative Medicine - ()

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