Ricerca: Ruolo del regolatore del citoscheletro inverted formin INF2 nella tumorigenesi del medulloblastoma
CV Marilisa Conenna
Nome e Cognome: Marilisa Conenna
Sede di Lavoro: Dipartimento di Medicina Molecolare, Viale Regina Elena 291
Tutor: Marella Maroder
Supervisor: Lucia Di Marcotullio
Precedenti esperienze di ricerca:
2015-2018: tesi triennale in scienze biologiche sperimentale presso il Dipartimento di biologia e biotecnologie "Charles Darwin". Nome della tesi "Study of intracellular trafficking of misfolded and retained proteins in the endoplasmatic reticulum"
2019-2020: tesi magistrale in Genetica e biologia molecolare presso il Dipartimento di medicina molecolare. Nome delle tesi "The role of the RNA-binding ubiquitin Ligase MEX3A in the degradation of oncosoppressor RIG-I: a new molecular mechanism involved in Glioblastoma tumorigenesis".
Formazione:
2015-2018: tesi triennale in scienze biologiche sperimentale presso il Dipartimento di biologia e biotecnologie "Charles Darwin"2019-2020: tesi magistrale in Genetica e biologia molecolare presso il Dipartimento di medicina molecolare.
2020-oggi: PhD student presso il Dipartimento di medicina molecolare.
Competenze: Data analysis: Adobe Photoshop, Image J, Image Lab, GraphPad. Use of various DataBases and tools: NCBI, PDB, BLAST, GenScript, OncoMX, Uniprot, Biorender, STRING.
Cell culture: Primary cells (murine medulloblastoma cells), immortalized murine and human cell lines.
Immunofluorescence and histology: Tissue preparation of histological samples: fixation, freezing, immunofluorescence. Cell preparation for immunofluorescence: fixation and permeabilization.
Molecular Biology techniques: Plasmidic and genomic DNA extraction, DNA mutagenesis, cloning, RNA extraction, reverse transcription, PCR, quantitative real time PCR, ubiquitination assays, gel electrophoresis, luciferase report assays.
Analysis of proteins: Protein extraction and western blot analysis, protein post-translational modifications analysis; in vivo and in vitro immunoprecipitation.
Cellular biology: DNA and siRNA transfection, cell treatments, BrdU and EDU assay, production and use of lentiviral vectors.
Animal (scientific procedures): manipulation of mice and mice’s brain tissues, tail cutting, toe clipping.