MARIANNA COSENTINO

Dottoressa di ricerca

ciclo: XXXIII


supervisore: Antonio Musarò

Titolo della tesi: X-MET, a muscle engineered tissue as a tool for basic research and regenerative medicine

Tissue engineering is an emerging field of research focused on the development of bioartificial substitutes for organs and tissues, which can, in turn, be applied in regenerative medicine, pharmaceutical, diagnostic, and basic research to elucidate fundamental mechanisms of cell functions and to investigate the molecular mechanisms underpinning disease onset and progression. We have previously generated an in vitro bioengineered three-dimensional vascularized skeletal muscle tissue, named eX-vivo Muscle Engineered Tissue (X-MET), which displays several morpho-functional properties of an in vivo muscle; it is able to contract spontaneously as well as to respond to electrical stimulation. The general aim of the thesis’s project was to further define, throughout three specific aims, the mechanical properties of X-MET and to disclose the functional plasticity of X-MET, subjected to mechanical stimuli. At first, we characterized the maximum shortening velocity, the maximum power, and the values of force and velocity at which X-MET develops the maximum power. Secondly, we contributed to better define the potential use of X-MET as an in vitro model for the preliminary testing and screening of drugs and molecules involved in disease onset and progression. We cultured X-MET under different conditions, mimicking cancer cachexia and aging and analyzed the molecular mechanisms involved in the myogenic program and in the maintenance of the muscle phenotype. Finally, based on the evidence that X-MET shows a synchronous and spontaneous contraction, we defined the functional plasticity of X-MET triggered by mechanical stimuli. Of note, it was observed that the 3D culture system and the applied mechanical tension induce the onset of cardiac muscle stiffness within X-MET, triggering the switch/remodeling from skeletal muscle to cardiac-like phenotype. Interestingly, transplanted X-MET was able to functional rescue the infarcted myocardium. For these features, X-MET can be considered a useful experimental tool for in vitro and in vivo studies to analyze homeostatic processes in a more complex in vitro model, for drug screening, and in regenerative medicine to restore, maintain, or improve tissue function.

Produzione scientifica

11573/1712884 - 2024 - Development of an optical system for strain drop measurement of osteosarcoma cells on substrates with different stiffness
Apa, L.; Martire, M. V.; Carraro, S.; Cosentino, M.; Del Prete, Z.; Peruzzi, B.; Rizzuto, E. - 01a Articolo in rivista
rivista: SENSORS (Basel : Molecular Diversity Preservation International (MDPI), 2001-) pp. 1-18 - issn: 1424-8220 - wos: WOS:001246987900001 (0) - scopus: 2-s2.0-85195942082 (0)

11573/1684900 - 2023 - Cyclin D3 deficiency promotes a slower, more oxidative skeletal muscle phenotype and ameliorates pathophysiology in the mdx mouse model of Duchenne muscular dystrophy
Bonato, A.; Raparelli, G.; Luvisetto, S.; Forconi, F.; Cosentino, M.; Tirone, F.; Rizzuto, E.; Caruso, M. - 01a Articolo in rivista
rivista: THE FASEB JOURNAL (Federation of American Societies for Experimental Biology (FASEB):9650 Rockville Pike, Room L-2310:Bethesda, MD 20814:(800)433-2732, (301)530-7027, EMAIL: staff@dues.faseb.org, INTERNET: http://www.faseb.org, Fax: (301)571-5728, (301)530-7001) pp. - - issn: 0892-6638 - wos: WOS:001007525300001 (0) - scopus: 2-s2.0-85162164662 (0)

11573/1689660 - 2023 - Modelling three-dimensional cancer-associated cachexia and therapy: the molecular basis and therapeutic potential of interleukin-6 transignalling blockade
Cosentino, M.; Forcina, L.; Zouhair, M.; Apa, L.; Genovese, D.; Boccia, C.; Rizzuto, E.; Musaro', Antonio - 01a Articolo in rivista
rivista: JOURNAL OF CACHEXIA, SARCOPENIA AND MUSCLE (Heidelberg ; Berlin : Springer) pp. 1-19 - issn: 2190-5991 - wos: WOS:001067904400001 (0) - scopus: 2-s2.0-85171443919 (0)

11573/1685226 - 2023 - Remodeled eX vivo muscle engineered tissue improves heart function after chronic myocardial ischemia
Cosentino, Marianna; Nicoletti, Carmine; Valenti, Valentina; Schirone, Leonardo; Di Nonno, Flavio; Apa, Ludovica; Zouhair, Mariam; Genovese, Desiree; Madaro, Luca; Dinarelli, Simone; Rossi, Marco; Del Prete, Zaccaria; Sciarretta, Sebastiano; Frati, Giacomo; Rizzuto, Emanuele; Musarò, Antonio - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. 1-15 - issn: 2045-2322 - wos: WOS:001059061400073 (2) - scopus: 2-s2.0-85163349344 (2)

11573/1656751 - 2022 - The development of an innovative embedded sensor for the optical measurement of ex-vivo engineered muscle tissue contractility
Apa, Ludovica; Cosentino, Marianna; Forconi, Flavia; Musarò, Antonio; Rizzuto, Emanuele; Del Prete, Zaccaria - 01a Articolo in rivista
rivista: SENSORS (Basel : Molecular Diversity Preservation International (MDPI), 2001-) pp. 1-15 - issn: 1424-8220 - wos: WOS:000856744500001 (3) - scopus: 2-s2.0-85138778040 (4)

11573/1656757 - 2022 - Effects of ROI positioning on the measurement of engineered muscle tissue contractility with an optical tracking method
Forconi, F.; Apa, L.; Cosentino, M.; Musaro', Antonio; Rizzuto, E.; Del Prete, Z. - 04b Atto di convegno in volume
congresso: 17th IEEE International Symposium on Medical Measurements and Applications, MeMeA 2022 (Messina)
libro: 2022 IEEE International Symposium on Medical Measurements and Applications, MeMeA 2022 - Conference Proceedings - (978-1-6654-8299-8)

11573/1556137 - 2021 - Electric field distribution analysis for the design of an electrode system in a 3D neuromuscular junction microfluidic device
Forconi, Flavia; Apa, Ludovica; D'alvia, Livio; Cosentino, Marianna; Rizzuto, Emanuele; Del Prete, Zaccaria - 04b Atto di convegno in volume
congresso: MeMeA 2021 - 2021 IEEE International Symposium on Medical Measurements and Application (Viertual)
libro: MeMeA 2021 - 2021 IEEE International Symposium on Medical Measurements and Applications Proceedings - (978-1-6654-1914-7)

11573/1469101 - 2020 - Mechanisms regulating muscle regeneration: insights into the interrelated and time-dependent phases of tissue healing
Forcina, L.; Cosentino, M.; Musaro', A. - 01g Articolo di rassegna (Review)
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. 1-28 - issn: 2073-4409 - wos: WOS:000539340200231 (121) - scopus: 2-s2.0-85085538839 (128)

11573/1262773 - 2019 - Measuring the maximum power of an ex vivo engineered muscle tissue with isovelocity shortening technique
Pisu, Simona; Cosentino, Marianna; Apa, Ludovica; Musarò, Antonio; Rizzuto, Emanuele; Del Prete, Zaccaria - 01a Articolo in rivista
rivista: IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT (IEEE / Institute of Electrical and Electronics Engineers Incorporated:445 Hoes Lane:Piscataway, NJ 08854:(800)701-4333, (732)981-0060, EMAIL: subscription-service@ieee.org, INTERNET: http://www.ieee.org, Fax: (732)981-9667) pp. 1-8 - issn: 0018-9456 - wos: WOS:000471078900011 (4) - scopus: 2-s2.0-85067110738 (5)

11573/1141643 - 2018 - Measuring the X-MET’s maximum power: a preliminary study
Pisu, Simona; Apa, Ludovica; Cosentino, Marianna; Musaro, Antonio; Rizzuto, Emanuele; Del Prete, Zaccaria - 04b Atto di convegno in volume
congresso: 2018 IEEE International Symposium on Medical Measurements and Applications (MeMeA) (Roma, Italia)
libro: MeMeA 2018 - 2018 IEEE International symposium on medical measurements and applications, Proceedings - (97815386-33922; 9781538633915)

11573/1176935 - 2017 - DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation
Latella, Lucia; Dall’Agnese, Alessandra; Boscolo Sesillo, Francesca; Nardoni, Chiara; Cosentino, Marianna; Lahm, Armin; Sacco, Alessandra; Pier Lorenzo Puri, And - 01a Articolo in rivista
rivista: GENES & DEVELOPMENT (Cold Spring Harbor Laboratory:500 Sunnyside Boulevard:Woodbury, NY 11797:(800)843-4388, (516)422-4100, EMAIL: cshpress@cshl.org, INTERNET: http://www.cshl.org, Fax: (516)422-4097) pp. 648-659 - issn: 0890-9369 - wos: WOS:000400068800005 (22) - scopus: 2-s2.0-85018292898 (23)

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