Phd in MOLECULAR MEDICINE

Research: Dissecting new functions of the Nijmegen breakage syndrome gene in cerebellar development

WORK ADDRESS:
Department of Molecular Medicine (RM110), Viale Regina Elena 291, Rome

SUPERVISOR:
Prof. Giuseppe Giannini

PREVIOUS RESEARCH EXPERIENCE:
September 2020 – July 2021: Student internship in the Molecular Oncology laboratory. Department of Molecular Medicine, Sapienza University of Rome.

Research lines: Role of Nbs1 in cerebellar development and carcinogenesis

CAREER:
2021 – today: PhD Student in Molecular Medicine at Department of Molecular Medicine, Sapienza University of Rome.
2018 – 2021: Master’s degree in Genetics and molecular biology (LM-6) at University of Rome. Graduation cum laude.
September 2019 – February 2020: Erasmus +, Universitat de Barcelona
2015 – 2018: Bachelor’s degree in Biology (L-13) at University of Rome. Graduation cum laude


SKILLS:
- Maintenance of immortalized cell cultures
- Generation of primary cultures
- Use of cryostat and staining of cryosections
- Immunohistochemistry
- Immunofluorescence
- Optical microscopy
- Widefield and confocal microscopy
- Transient transfection
- Infection with virus
- RNA and DNA extraction and purification
- Separation of nucleic acids by electrophoresis
- PCR, RT-PCR, Real Time PCR
- Protein extraction
- Western Blot
- Immunoprecipitation
- Protein fraction isolation
- Proliferative Assays (EdU)