Research: PEN2 as a novel metabolic regulator of lysosomal function in colorectal cancer
Personal data:
First and Last Name: Luca Campea
Date and place of birth: 21/07/1999, Rome (RM), Italy
Email: luca.campea@uniroma1.it
Work address:
Laboratory of Molecular Oncology (Department of Molecular Medicine) - Epigenetics and Metabolism Unit - Viale Regina Elena 291, 00161 Rome, Italy
Supervisor: Professor Gianluca Canettieri
Career:
July 2023: Master's degree in Genetics and Molecular Biology, Sapienza - University of Rome.
Grade: 110/110 cum laude
Thesis title: "Metformin counteracts colorectal cancer growth by NADH-mediated activation of the oncosuppressor PEN2"
Supervisor: Prof. Gianluca Canettieri (Department of Molecular Medicine - Sapienza)
October 2021: Bachelor's degree in Biological Sciences, Sapienza - University of Rome.
Grade: 110/110 cum laude
Thesis title: "Role of oxidative metabolism in neural stem cell immortalization during tumorigenesis"
Supervisor: Prof. Laura Ciapponi (Department of Biology and Biotechnology "C. Darwin" - Sapienza)
Research Experience:
November 2023 - Today: PhD student in Molecular Medicine, Department of Molecular Medicine - Sapienza, University of Rome (Italy).
Tutor: Professor Gianluca Canettieri.
December 2020 - July 2023: Internship at the Department of Molecular Medicine - Sapienza, University of Rome (Italy).
Tutor: Prof. Gianluca Canettieri
Skills:
- Cell Biology: Cell culture techniques (human and animal cells), DNA and siRNA transfection and transduction, drug treatments, cell proliferation and viability assays, production and use of lentiviral vectors.
- Molecular biology techniques: Plasmid and genomic DNA extraction, RNA extraction, cloning, DNA mutagenesis, retro-transcription, PCR, quantitative real time PCR, luciferase reporter activity assay, Chromatin immunoprecipitation (ChIP)
- Protein analysis: Protein extraction and Western blot, in vivo and in vitro immunoprecipitations.
- Cytofluorimetry techniques.
- Animal experimental procedures: Handling and collection of tissue samples from mice. Marking techniques. Analgesia, anesthesia. FELASA Accredited Course F 023/09 "Laboratory Animal Science" 2023 (#28/2023) - Functions A, B, C, D.
- Computer skills: Office package; Adobe Photoshop; Image J; Image Lab; GraphPad. Use of various DataBases and tools: NCBI, PDB, BLAST, GenScript, OncoMX, GEPIA, PyMOL, GPower Uniprot, Biorender, STRING.
Training Courses:
November 2023: FELASA Accredited Course F 023/09 in "Laboratory Animal Science" at Fondazione Santa Lucia IRCCS, Rome - Italy. For Functions A, B, C, D. Species: Mouse and Rat.