LOREDANA IANNELLA

PhD Graduate

PhD program:: XXXIII



Thesis title: Forensic application of isotope ratio mass spectrometry: development and validation of GC-C-IRMS based methods in doping analysis

The isotope ratio mass spectrometry coupled to gas chromatography (GC-C-IRMS) is a mandatory procedure in anti-doping analyses performed on urinary samples in which the concentration and/or the ratio concentrations of specific steroid profile markers overcome the limits defined by the World Anti-Doping Agency (WADA) or deviate from the population and individual reference range. It is a confirmatory investigation to establish whether these alterations, if not caused by physiological reasons, can be attributed to a synthetic steroids’ intake. Indeed, the determination of their 13C composition, which is typically more depleted for pharmaceutical preparations compared to their physiologically produced counterparts, allows disclosing the exogenous or endogenous origin of the steroid compounds. In this Thesis, novel approaches have been implemented to simplify, standardize the current operating protocols, and reduce the risk of misinterpreting the analytical findings. The benefits of performing a large volume injection by a programmed temperature vaporizer inlet have been proved in analyzing steroids showing low urinary levels and new methods for detecting the prednisolone/prednisone, and 19-norsteroids abuse have been developed and fully validated. In order to provide a detailed overview of the IRMS technique, some criticalities have been also emphasized, proposing, whenever possible, suitable troubleshooting.

Research products

11573/1194718 - 2018 - Detection of prohibited growth factors in doping control: serum concentration ranges in a reference population of athletes
Donati, Francesco; Vannelli, Emanuela; Iannella, Loredana; De La Torre, Xavier; Botre', Francesco - 04b Atto di convegno in volume
conference: XXXVI Cologne Workshop on Dope Analysis (Colonia, Germania)
book: RECENT ADVANCES IN DOPING ANALYSIS - ()

11573/1194745 - 2018 - Large volume injection: improving the detection capacity of endogenous anabolic steroids by GC-C-IRMS
Iannella, Loredana; Colamonici, Cristiana; Curcio, Davide; Botre', Francesco; De La Torre, Xavier - 04f Poster
conference: XXII International Mass Spectrometry Conference (Firenze, Italia)
book: XXII International Mass Spectrometry Conference - ABSTRACT BOOK - ()

11573/1194760 - 2018 - Isotopic metabolic fractionation of endogenous steroids by 5α-reductase inhibitors
Iannella, Loredana; Colamonici, Cristiana; Curcio, Davide; Botre', Francesco; De La Torre, Xavier - 04d Abstract in atti di convegno
conference: The 2nd Isotope Ratio MS Day (Messina, Italia)
book: 2nd Isotope Ratio MS Day - ABSTRACT BOOK - ()

11573/1121242 - 2017 - A multiplexed bead-based flow cytofluorimetric assay for the screening of prohibited growth factors in doping control
Donati, Francesco; Caporuscio, Dario; Serafini, Sebastiano; Calzoni, Fabiola; Iannella, Loredana; Pirri, Daniela; De La Torre, Xavier; Botre', Francesco - 04b Atto di convegno in volume
conference: XXXV Cologne Workshop on Dope Analysis (Colonia, Germania)
book: RECENT ADVANCES IN DOPING ANALYSIS - ()

11573/1121270 - 2017 - Genotypic variability and doping control: effects of the polymorphism of the family of UGT2B genes on the urinary excretion of anabolic steroids in a reference population
Donati, Francesco; Mascia, Elena; Orlandi, Stefano; Stampella, Alessandra; Pirri, Daniela; Iannella, Loredana; De La Torre, Xavier; Botre', Francesco - 04b Atto di convegno in volume
conference: XXXV Cologne Workshop on Dope Analysis (Colonia, Germania)
book: RECENT ADVANCES IN DOPING ANALYSIS - ()

11573/1362645 - 2015 - Liposomes as drug delivery system in sport doping recent advances in the study of liposome-vehiculated hemoglobins
Colicchia, Sonia; Botre', Francesco; De La Torre, Xavier; Esposito, Simone; Iannella, Loredana; Donati, Francesco - 04d Abstract in atti di convegno
conference: 53rd TIAFT meeting (Firenze; Italy)
book: 53rd TIAFT meeting, August 30th - September 4th, Firenze, Italy - ()

11573/615584 - 2013 - Fast screening for liposomes encapsulated hemoglobins (LEH) using ADVIA 120 flow cytomery-based hematological analyzer
Donati, F.; Colicchia, S.; De La Torre, X.; Iannella, L.; Botre', Francesco - 04b Atto di convegno in volume
conference: MANFRED DONIKE WORKSHOP (COLONIA)
book: RECENT ADVANCES IN DOPING ANALYSIS (21) - (978-3-86884-039-1)

11573/539849 - 2013 - Fast screening for liposome encapsulated hemoglobin (LEH) using ADVIA 120 flow cytometry-based hematological analyzer
Donati, Francesco; Colicchia, Sonia; De La Torre, X.; Iannella, L.; Botre', Francesco - 04b Atto di convegno in volume
conference: Manfred Donike Workshop (Cologne)
book: Recent Advances in Doping Analysis - ()

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma