Research: The role of the microRNAs in Cxcr4-dependent maturation of thymocytes in a Notch3-induced Acute Lymphoblastic Leukemia model
PERSONAL INFORMATION
Name and surname: ILARIA SERGIO
Address: Via Livorno 45, 00162, Rome, Italy
Phone: 0039 3479128219
E-mail: Ilaria.sergio@uniroma1.it
Nationality: Italian
Date of Birth: 20/02/1997
EDUCATION AND TRAINING
1/11/2021-on going: PhD in Molecular Medicine
University of Rome La Sapienza, piazzale Aldo Moro 5, 00185, Rome (Italy)
1/11/2020-21/10/21: Experimental Thesis
Department of Molecular Medicine, Viale Regina Elena 291, 00161, Rome (Italy)
01/10/2019-21/10/2021: Master degree in Genetics and Molecular Biology
University of Rome La Sapienza, piazzale Aldo Moro 5, 00185, Rome (Italy)
Thesis in "Role of microRNAs in CXCR4-dependent maturation of thymocytes in a model of leukemia induced by Notch3"
01/09/15-26/07/2019: Bachelor Degree in Biological Sciences
Polytechnic University of Marche, via Brecce Bianche, 60131, Ancona (Italy)
Thesis in "Regulation of intestinal absorption of Vitamin E".
01/01/2018-31/05/2018: Curricular internship
Presidio Ospedaliero "G. Salesi", via Filippo Corridoni 11, 60131, Ancona (Italy)
2010-2015: Diploma
Liceo scientifico "Teodoro Monticelli" Brindisi, via Nicola Brandi 22, 72100, Brindisi (Italy)
Five-year diploma
PERSONAL SKILLS AND COMPETENCES
- Excellent learning skills
- Excellent teamwork
- Ability to move within a laboratory
- Ability to use laboratory materials
- Experience with RNA/DNA and protein extraction
- PCR and qRT-PCR analysis
- Experience in cell culture
- Experience in basic molecular biology techniques
- Experience in functional genomics (miRNA)
- Western blot technique
- Cell Transfection Technique (Neon System Transfection)
- Flow cytometry analysis
- Cell sorting by FACs Sorting
- Processing of organs and tissues from genetically modified mouse models
- Pharmacological Treatments
TRAINING COURSE
- Certificate obtained on 06/112021 for the Training Course in "Basic elements for the approach of researchers to the use of animals for scientific purposes".
- "Training and refresher course for the protection of laboratory animals in scientific research", VI Edition from 10 October 2022 to 21 October 2022 at the Experimental Research Centre of the Università Cattolica del Sacro Cuore.
Mother languages: Italian
Other languages: English B2
COMPUTER SKILLS:
- In-depth knowledge of Windows, OsX platforms.
- Excellent knowledge of the Office package and ability in databases both regarding the design, implementation and storage.
- Excellent knowledge of email services and of the most common browsers.
- Ability to use the program Pymol for the visualization of the structure of proteins.
- Expertise in the use of ImageJ.
- Expertise in the use of CellQuester and FlowJo.
- Statistical skills with SPSS and ANOVA.
- Bioinformatics skills.
PARTICIPATION
- 10/05/22: Seminar "Precision Immunoncology: patient identikit to guide therapeutic choices".
- 12/04/22: Seminar "Multiple Myeloma: modulation of the tumour microenvironment and strategies for stimulating the immune system".
- 29/03/22: Seminar "The role of autophagy in cardiovascular diseases".
- 01/02/22: Seminar "Notch1 controls metabolic switches in the G2/Damage checkpoint and Responses to stress".
WORKSHOP E CONGRESS
- 28-29/11/22: Worshop: “Bridging Immunity to Cancer: Models of Study and New Research Outcomes”.
- 28-29/03/22: 4th international workshop on tumor evolution: “Making the Impossible Possible: Successful Tackling of Difficult Targets in Cancer Research and Clinical Development”.
- 20-23/06/22: “EACR 2022 Congress Siviglia”
- 01-05/10/23: Partecipation in “The Notch Meeting” congress in Athens with poster presentation “Notch3 deregulates CXCR4 expression in immature thymocytes to sustain Acute Lymphoblastic Leukemia progression”.
FINANCED RESEARCH GRANTS
2023-2024: Winner of funding for the research project entitled: “Inhibitors of anti-apoptotic Bcl-2 proteins in the treatment of Notch-dependent T-cell Acute Lymphoblastic Leukemia”. Project number AR123188AF112A64 funded by Avvio alla Ricerca-Ateneo Sapienza.
2023-2024: Winner of the funding of individual research projects for mobility abroad whose title is “The Role of microRNAs during T-ALL progression” funded by MIUR_PhD_2023 which takes place in the Centro de Biología Molecular “Severo Ochoa”, CSIC-Universidad Autónoma de Madrid (UAM).
2022-2023: Participant of project titled “Notch as a mediator of lympho-stromal interactions in T-ALL.”. Project number AR222181616D9D01, funded by Avvio alla Ricerca-Ateneo Sapienza.
2022-2023: Participated of project titled “Notch in the progression of acute T-cell lymphoblastic leukemia: emerging role of microRNAs in this complex disease.” PI Prof. Maria Pia Felli. Project number RP122181642E92CE, funded by Avvio alla Ricerca-Ateneo Sapienza.
PUBLICATIONS IN INTERNATIONAL SCIENTIFIC JOURNALS
- Del Gaizo, M., Sergio, I., Lazzari, S., Cialfi, S., Pelullo, M., Screpanti, I., & Felli, M. P. (2022). MicroRNAs as Modulators of the Immune Response in T-Cell Acute Lymphoblastic Leukemia. International Journal of Molecular Sciences, 23(2), 829.
Del Gaizo M. e Sergio I. sono primi co-autori.
- Valentini E, Di Martile M, Brignone M, Di Caprio M, Manni I, Chiappa M, Sergio I, Chiacchiarini M, Bazzichetto C, Conciatori F, D'Aguanno S, D'Angelo C, Ragno R, Russillo M, Colotti G, Marchesi F, Bellone ML, Dal Piaz F, Felli MP, Damia G, Del Bufalo D. Bcl-2 family inhibitors sensitize human cancer models to therapy. Cell Death Dis. 2023 Jul 17;14(7):441. doi: 10.1038/s41419-023-05963-1. PMID: 37460459; PMCID: PMC10352371.
- Sandesh Kumar Patel1†, Nadezda Zhdanovskaya1†#, Ilaria Sergio2, Antonella Cardinale3, Marco Rosichini3, Franco Locatelli3,4, Alberto Macone5, Enrico Velardi3, Rocco Palermo1, Maria Pia Felli2*. Thymic epithelial cell-dependent microenvironment influences proliferation and apoptosis of leukemic cells.
- Sergio, I., Del Gaizo, M., Patel, S., Varricchio, C., Russo, E., ... & Felli, M. P. (2023). Notch3-regulated microRNAs impair CXCR4-dependent maturation of thymocytes allowing maintenance and progression of T-ALL.
Submitted on Oncogene, under revision.
I authorize the processing of my personal data in accordance with Legislative Decree 196 of 30 June 2003.
Signature
Dr. Ilaria Sergio