Research: Study of the role of Hedgehog signaling pathway and its negative modulator KCASH2 in mouse colon carcinogenesis
WORK ADDRESS: Molecular Oncology Laboratory –Department of Molecular Medicine – Viale Regina Elena 291, 00161, Rome, Italy
SUPERVISOR: Professor Enrico De Smaele
PREVIOUS RESEARCH EXPERIENCE:
INTERNSHIP FOR MASTER'S DEGREE IN PHARMACEUTICAL BIOTECHNOLOGY
At the Laboratory of Molecular Oncology (Department of Molecular Medicine), La Sapienza University of Rome, Rome
Title of the project: "The role of HECT-E3 Ubiquitin Ligases SMURF1 and SMURF2 in the modulation of the Hedgehog signaling pathway".
INTERNSHIP FOR BACHELORS' DEGREE IN BIOTECHNOLOGY
At the Italian Biochemical Institute Giovanni Lorenzini (IBI), Spa, Aprilia
Project title: “Engineering, production and activity of the metal beta-lactamase Vim-1 of P. aeruginosa”.
CAREER:
December 2022 Attended the training course “Preclinical Trials and Animal
Welfare Training “ (FSPBA) This course is mandatory to
perform the functions A provided by art. 23 par. 2 d.lgs.
26/2014, training for personnel who work with animals for
scientific purposes according to d.lgs of 5 August2021 and
DD of 18 March 2022, “La Sapienza” University Rome.
October 2022 – today PhD student in Molecular Medicine (XXXVIII cycle),
Molecular Oncology Laboratory – Department of
Molecular Medicine “La Sapienza” University of Rome
Supervisor: Professor Enrico De Smaele
October 2020– October 2022 Masters’ Degree in Pharmaceutical Biotechnology
Faculty of Pharmacy and Medicine
“La Sapienza” University of Rome
Thesis: “ The role of HECT-E3 Ubiquitin Ligases
SMURF1 and SMURF2 in the modulation of Hedgehog
signaling pathway”
Supervisor: Professor Enrico De Smaele
Grade: 110/110 cum Laude
October 2016 – 2020 Bachelors’ Degree in Biotechnology
University of Perugia
Thesis: “Engineering, production and activity of Metallo
beta-Lactamase Vim-1 P.aeruginosa,”
At: Giovanni Lorenzini Italian Biochemical Institute
(IBI),S.p.a, Aprilia
Grade: 100/110
October 2019 – April 2019 Practical training
At: Giovanni Lorenzini Italian Biochemical Institute
(IBI),S.p.a, Aprilia
TRAINING COURSES AND RESEARCH EXPERIENCE
October 2022 – today
Scientific research activity as a Molecular Medicine PhD student, Molecular Oncology Laboratory –Department of Molecular Medicine – “La Sapienza” University of Rome.
- Study of Smurfs protein role in the negative regulation of Hedgehog signaling pathway
and in medulloblastoma
- Characterization of Smurfs and Gli1 interaction.
December 2022
Attended the training course “Preclinical Trials and Animal Welfare Training “ (FSPBA) This course is mandatory to perform the functions A provided by art. 23 par. 2 d.lgs. 26/2014, training for personnel who work with animals for scientific purposes according to d.lgs of 5 August2021 and DD of 18 March 2022, “La Sapienza” University Rome.
CONGRESS PARTECIPATION
2023- Poster presentation ABCD National Congress 2023, Paestum, Italy.
SCIENTIFIC FUNDINGS
2023- Awarded the Sapienza “University research starting grant” for the project: Histological and molecular analysis of the role of KCASH2 in the colon.
SCIENTIFIC PUBBLICATIONS
2023- Di Fiore A, Bellardinelli S, Pirone L, Russo R, Angrisani A, Terriaca G, Bowen M, Bordin F, Besharat ZM, Canettieri G, Fabretti F, Di Gaetano S, Di Marcotullio L, Pedone E, Moretti M, De Smaele E. KCTD1 is a new modulator of the KCASH family of Hedgehog suppressors. Neoplasia. 2023
SKILLS:
Technical skills:
- Genotyping and classic PCR
- Maintenance and pharmacological treatment of primary and immortalized cell cultures
- Immunofluorescence staining of cells
- Transient transfection of nucleic acids with electroporation or liposomic agents
- RNA and DNA extraction and purification, RT-PCR and Q-PCR
- Protein extraction, Bradford Assay, Western Blot
- Immunoprecipitation and Ubiquitination Assays
- Cultivation and isolation of bacterial cultures, bacterial transformation and DNA purification techniques
- Bacterial cloning
- Clonogenic Assays and Proliferative Tests: BrdU/EdU
OTHER SKILLS:
- Excellent organizational and management skills of own work
- Good ability to work in an interactive group, to operate with a certain degree of autonomy and to be readily integrated into the work environment
- Great ability to communicate and adapt to multicultural environments acquired during university studies and work experience
LANGUAGE SKILLS:
English
Comprehension (Listening and Reading) B1; Speaking (Oral interaction and Oral production) B1; Writing B1
COMPUTER SKILLS:
Operating Systems (Good), Programming (Fair), Word Processing (Good), Spreadsheets (Good), Database Managers (Fair), Internet Browsing (Good), Website Creation (Good), Multimedia ( sounds, images, videos) (Good),
Programming Languages: (C++, Javascript)