Research: Crosstalk between PLK1 and NOTCH1 in the regulation of DNA damage-induced cell cycle arrest and survival in cancer cells
WORKPLACE: PhD student at the Department of Molecular Medicine – Molecular Pathology Laboratory – Viale Regina Elena, 291 Building B, 4th floor. Sapienza University of Rome.
Supervisor: Prof. Claudio Talora
PREVIOUS RESEARCH EXPERIENCE:
Internship at the Microbial Biotechnology Laboratory, Department of Biology and Biotechnology “Charles Darwin”, Sapienza University of Rome.
Tutor: Prof. Daniela Uccelletti (10/2023 – 10/2024).
Research project: Evaluation of potential positive effects of plant extracts on the model organism Caenorhabditis elegans.
Collaboration grant at the Chemical Laboratory, Sapienza University of Rome (10/2023 – 01/2024).
Collaboration grant at the Biochemical Laboratory, Sapienza University of Rome (04/2024 – 10/2024).
EDUCATION:
Master’s Degree in Biotechnology and Genomics for Industry and the Environment
(LM-8).
Sapienza University of Rome [10/2022 – 10/2024]
Field of study: Microbial Biotechnology
Final grade: 110/110 with Honors
Experimental Thesis: Plant extracts as a biotechnological resource: effects of the tomato supply chain on the model organism Caenorhabditis elegans.
Tutor: Prof. Daniela Uccelletti
Bachelor’s Degree in Biotechnology
[L-270 - Ordin. 2019] (classe L-2).
Sapienza University of Rome [10/2019 – 10/2022]
Field of study: Microbiology, Molecular Biology
Final grade: 107/110
Experimental Thesis: Selection of Staphylococcus epidermidis strains able to inhibit or disrupt biofilm produced by S. aureus.
Tutor: Prof. Maria Pia Conte
High School Diploma in Scientific Studies
Scientific High School “Leonardo Da Vinci” Sora (FR) [09/2014 – 06/2019]
Final grade: 90/100
HOLDINGS:
02/12/2024-04/12/2024: CONGRESS: 36° AICC International Meeting 2024
Hijacking good pathways: cancer, immunity and therapeutic approaches.
University of Rome La Sapienza.
SKILLS:
Molecular Biology Techniques: DNA and RNA extraction. Protein extraction from cells, purification, quantification, and Western Blot analysis. Nucleic acid separation on agarose gel, PCR (Polymerase Chain Reaction), RT-PCR (Reverse Transcriptase–Polymerase Chain Reaction), Real-Time RT-PCR. Solution preparation.
Cell Biology Techniques: Basic techniques, including culture media preparation, cell line expansion, freezing and thawing, in vitro pharmacological treatments, application of gene silencing techniques. Bacterial transformation and growth, plasmid purification, and transfection in cell lines. Knowledge and hands-on experience with the model system C. elegans.
Equipment: Use of centrifuges, autoclave, spectrophotometer, analytical balance, thermocycler, pH meter, stereomicroscope, optical microscope, Soxhlet extractor, Real-Time PCR system, Western Blotting equipment, chemical and biological safety cabinets, and cell biology laboratory equipment. Statistical skills with SPSS and ANOVA. Bioinformatics skills (Pymol). Flow cytometry analysis.
Proficient in software applications such as Microsoft and Office suite.