Dottoressa di ricerca

ciclo: XXXIV

Titolo della tesi: Role of multidrug resistance efflux pumps during intracellular life of Adherent-Invasive Escherichia coli strain LF82

Efflux pumps (EPs) are inner membrane proteins found in all organisms, from bacteria to humans, and are involved in the transport process of the cell. EPs mediate the transport of many types of molecules and contribute to the development of multidrug resistance (MDR) thanks to their ability to transport simultaneously different antibiotics. To date an increasing number of works, indicate that, besides their role in the antibiotics transport, MDR EPs play an important role in several cellular functions such as biofilm formation, stress response, maintenance of cellular homeostasis and detoxification of cell from metabolic intermediates. They also actively contribute to the bacterial interaction with plant and animal cells, and to the virulence of many pathogenic bacteria. This let us to consider their role in the intracellular life of our model system, LF82, the prototype strain of Adherent Invasive Escherichia coli (AIEC). AIEC is a group of enteropathogenic E. coli, capable of colonizing the ileal mucosa of patients with Crohn's disease (CD) causing strong intestinal inflammation through a sophisticated infectious strategy. Indeed, AIEC strains are able to invade and replicate in epithelial cells and macrophages, where they replicate extensively in large vacuoles without inducing apoptosis. The aim of my PhD thesis was to analyze the expression profile of the genes encoding the MDR EPs present in the LF82 genome during macrophage and epithelial cell infection in order to identify which MDR EPs were relevant during the survival within host cells or in the early stages of the invasive process. The data I obtained demonstrates for the first time that MDR EPs are involved in the invasive process and contribute to the intracellular life of AIEC strains and open new perspectives in the study of AIEC interactions with host cells

Produzione scientifica

11573/1679484 - 2023 - Role of the MDR Efflux Pump AcrAB in Epithelial Cell Invasion by Shigella flexneri
Coluccia, Marco; Béranger, Aude; Trirocco, Rita; Fanelli, Giulia; Zanzi, Francesco; Colonna, Bianca; Grossi, Milena; Prosseda, Gianni; Pasqua, Martina - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. - - issn: 2218-273X - wos: WOS:000994273500001 (3) - scopus: 2-s2.0-85160625326 (3)

11573/1673186 - 2023 - AcrAB efflux pump impacts on the survival of adherent-invasive Escherichia coli strain LF82 inside macrophages
Fanelli, G.; Pasqua, M.; Prosseda, G.; Grossi, M.; Colonna, B. - 01a Articolo in rivista
rivista: SCIENTIFIC REPORTS (London: Springer Nature London: Nature Publishing Group) pp. - - issn: 2045-2322 - wos: WOS:001012758100044 (8) - scopus: 2-s2.0-85148114702 (8)

11573/1567231 - 2021 - Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family
Pasqua, M.; Bonaccorsi Di Patti, M. C.; Fanelli, G.; Utsumi, R.; Eguchi, Y.; Trirocco, R.; Prosseda, G.; Grossi, M.; Colonna, B. - 01a Articolo in rivista
rivista: FRONTIERS IN MOLECULAR BIOSCIENCES (Lausanne : Frontiers Media S.A., 2014-) pp. - - issn: 2296-889X - wos: WOS:000683011800001 (23) - scopus: 2-s2.0-85112170132 (28)

11573/1502194 - 2021 - Modulation of omv production by the lysis module of the dlp12 defective prophage of Escherichia coli k12
Pasqua, M.; Zennaro, A.; Trirocco, R.; Fanelli, G.; Micheli, G.; Grossi, M.; Colonna, B.; Prosseda, G. - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000622827700001 (12) - scopus: 2-s2.0-85100670662 (12)

11573/1487170 - 2020 - Gcn5p and ubp8p affect protein ubiquitylation and cell proliferation by altering the fermentative/respiratory flux balance in saccharomyces cerevisiae
De Palma, A.; Fanelli, G.; Cretella, E.; De Luca, V.; Palladino, R. A.; Panzeri, V.; Roffia, V.; Saliola, M.; Mauri, P.; Filetici, P. - 01a Articolo in rivista
rivista: MBIO (Washington, D.C. : American Society for Microbiology) pp. 1-16 - issn: 2150-7511 - wos: WOS:000572066000008 (4) - scopus: 2-s2.0-85089556827 (4)

11573/1436559 - 2020 - Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-Invasive Escherichia coli Strain LF82
Fanelli, G.; Pasqua, M.; Colonna, B.; Prosseda, G.; Grossi, M. - 01a Articolo in rivista
rivista: FRONTIERS IN MICROBIOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. - - issn: 1664-302X - wos: WOS:000567824700001 (12) - scopus: 2-s2.0-85090055487 (13)

11573/1311714 - 2019 - The Varied Role of Efflux Pumps of the MFS Family in the Interplay of Bacteria with Animal and Plant Cells
Pasqua, Martina; Grossi, Milena; Zennaro, Alessandro; Fanelli, Giulia; Micheli, Gioacchino; Barras, Frederic; Colonna, Bianca; Prosseda, Gianni - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000489131300012 (62) - scopus: 2-s2.0-85074357294 (68)

11573/1190325 - 2018 - Ubiquitin protease Ubp8 is necessary for S. cerevisiae respiration
Leo, Manuela; Fanelli, Giulia; Di Vito, Serena; Traversetti, Barbara; La Greca, Mariafrancesca; Palladino, Raffaele A.; Montanari, Arianna; Francisci, Silvia; Filetici, Patrizia - 01a Articolo in rivista
rivista: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL:, INTERNET:, Fax: 011 31 20 4853598) pp. 1491-1500 - issn: 0167-4889 - wos: WOS:000444929400009 (9) - scopus: 2-s2.0-85050979365 (9)

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