Research: Investigating the effect of RAD52 deregulation on pathways leading to single-stranded DNA gap accumulation
Education
PhD student in Human Biology and Medical Genetics 2023 – ongoing
La Sapienza Università di Roma – Istituto Superiore di Sanità
Study of replicative stress response in cancer cells for the purpose of identifying new therapeutic targets for anti-tumor therapy
Master’s Degree in Genetics and Molecular Biology 110/110 cum laude 2021 – 2023
La Sapienza Università di Roma
Thesis title: "A preliminary characterization of daughter strand gaps associated with RAD52 overexpession and SMARCAL1-deficiency and involvement in replication stress signaling"
Bachelor’s Degree in Biological Sciences – 110/110 2018 – 2021
Università Politecnica delle Marche
Thesis title: "The dimerization of protein 2 cyclase associated regulates cofilin in synaptic plasticity and in
Alzheimer's disease"
Istituto Superiore di Sanità – Roma Jul 2022 – Oct 2023 Human Genome Stability – https://www.iss.it/
Researcher and Thesis Student
• Study of the molecular mechanisms of RAD52.
• Study of replication fork remodeling mechanisms in immortalized human cells and human cancer cells.
• Study of repair mechanisms to DNA damage.
Università Politecnica delle Marche – Ancona Jan 2021 – Apr 2021
Developmental Biology – https://www.disva.univpm.it/ Stabularist Researcher Intern
• Studied developmental stages in Danio rerio.
• Monitored the optimal conditions for sample growth.
• Practiced laboratory histology techniques for the study of Zebrafish tissues and organs.
Attendance at conferences
36° AICC International Meeting 2024 2nd – 4th Dec 2024 Roma, Italy
HIJACKING THE <> PATHWAYS: CANCER, IMMUNITY AND THERAPEUTIC APPROACHES
Keystone Symposia 27th Apr – 1st May 2025 Daejeon Convention Center, Daejeon, , Republic of Korea
DNA REPLICATION GAPS, CANCER AND DISEASE
Abstract and poster session
Publications
• Honda M, Razzaghi M, Gaur P, Malacaria E, Marozzi G, Biagi LD, Aiello FA, Paintsil EA, Stanfield AJ, Deppe BJ, Gakhar L, Schnicker NJ, Ashley Spies M, Pichierri P, Spies M. A double-ring of human RAD52 remodels replication forks restricting fork reversal. bioRxiv [Preprint]. 2024 Sep 18:2023.11.14.566657. doi: 10.1101/2023.11.14.566657. Update in: Nature. 2025 May;641(8062):512-519. doi: 10.1038/s41586-025-08753-1. PMID: 38014173; PMCID: PMC10680749.
• Di Biagi L, Marozzi G, Malacaria E, Honda M, Aiello FA, Valenzisi P, Spies M, Franchitto A, Pichierri P. RAD52 prevents accumulation of Polα -dependent replication gaps at perturbed replication forks in human cells. bioRxiv [Preprint]. 2024 Aug 17:2023.04.12.536536. doi: 10.1101/2023.04.12.536536. PMID: 37090680; PMCID: PMC10120653.
Languages
Italian: Native ; English: Fluent; Spanish: Basic
IT Skills
ImageJ; GraphPad; ImageLab; R; SnapGene; CometScore; PyMol; quantitative microscopy; high-resolution microscopy;
Good knowledge of Office Suite (Excel: advanced; Power Point: advanced, Word: advanced)
Soft Skills
Listening attitude; flexibility and ability to change; proactive attitude; planning skills; propensity for teamwork; propensity to work for objectives.