Research: NADH shuttle systems as therapeutic target in colorectal cancer
WORK ADDRESS: Laboratory of Molecular Oncology (Department of Molecular Medicine), Viale Regina Elena 291, 00161 Rome, Italy
SUPERVISOR: Prof.ssa Marella Maroder
RESEARCH EXPERIENCE:
September 2021-October 2022: Internship at the Department of Molecular Medicine at La Sapienza University of Rome, Rome (Italy)
Supervisor: Prof Gianluca Canettieri
November 2022- Today: PhD student in Molecular Medicine, department of Molecular Medicine at La Sapienza University of Rome, Rome (Italy)
Supervisor: Prof.ssa Marella Maroder
CAREER:
2020- Bachelor's degree in Biotecnologie at La Sapienza University of Rome, Rome (Italy)
Score: 110/110
Thesis title: Ruolo della piridossina 5'-fosfato ossidasi nella via di recupero della vitamina B6
Supervisor: Prof Roberto Contestabile
2022- Master degree in Biotecnologie Mediche at La Sapienza University of Rome, Rome (Italy)
Score: 110/110 cum laude
Thesis title: L'inibizione della glicerolo-3-fosfato deidrogenasi mitocondriale come nuovo approccio farmacologico nella terapia del cancro del colon retto
Supervisor: Prof Gianluca Canettieri
2023- Training course “Sperimentazione Preclinica e Benessere Animale” at La Sapienza University of Rome, Rome (Italy)
SKILLS:
-CELLULAR BIOLOGY: cell culture techniqes (animal and human cells), DNA and SiRNA transfection/trasduction, cell treatments, cell viabilityand proliferation assay, production and use of lentiviral vectors.
-MOLECULAR BIOLOGY TECHNIQUES: plasmidic and genomic DNA extraction, RNA extraction, cloning, DNA mutagenesis, reverse trascription, PCR, quantitative real time PCR, luciferase reporter assay.
-ANALYSIS OF PROTEINS: protein extraction western blot anaòysis, immunoprecipitation.
-CYTOFLUOROMETRY TECHNIQUES
-DATA ANALYSIS: Adobe Photoshop, Image J, Image Lab, GraphPad.
-KNOWLEDGE OF DATABASES AND TOOLS: NCBI, PDB, BLAST, GenScript, OncoMX, Uniprot, Biorender, STRING.
-ANIMAL EXPERIMENTATION