GIADA PONTECORVI

Dottoressa di ricerca

ciclo: XXXIII



Titolo della tesi: Antitumoral activity of the Estrogen Receptor β agonist LY500307 against melanoma and new nanocarrier strategy for its pharmacological delivery

Worldwide the total incidence of cutaneous melanoma is higher in men than in women. In addition, localized melanomas had a lower propensity to metastasize in women, resulting in a better survival when compared with men. Although we still lack conclusive data explaining these differences, among others factors, a fundamental role appears played by sex hormones, suggesting a protective effect of estrogens. However, conflicting results exist about the beneficial action of estradiol and its analogues, at least in part related to the different action of Estrogen Receptors (ERs) α and β, favoring proliferation the former and exerting an antitumor function the latter. Here we investigated the effects of estrogen treatments as well as those of the ERβ specific agonist LY500307 in a series of melanoma cell lines characterized by different genetic background and stages. Our results showed that melanoma in vitro treatment with LY500307 induced a significant cell cycle accumulation at the G2/M boundary associated with anomalous mitotic structures and increased pro-apoptotic signals. Moreover, LY500307 treatment was able to decrease melanoma cell malignancy reducing in vitro cell migration and down-regulating the expression of some oncomiRs belonging to the miR-17 family. In view of LY500307 promising results and in order to exploit these studies on melanoma in vivo, we focused on the innovative therapeutic approach of nanocarrier-based drugs delivery. Since we previously unveiled the roles of oleic acid (OA) against melanoma in in vitro studies (Bellenghi M. et al, 2015; Puglisi R. et al, 2018), we generated OA-based nanovesicles according to a nanoemulsion formulation. After evaluating some key properties of these nanovesicles (i.e. size, stability), we confirmed their good uptake through a Nile Red staining in metastatic melanoma, and any effect on normal cell viability. Although in vivo studies in proper animal models should be run before any possible clinical translation, these results allowed hypothesizing the in vivo long-term therapeutic potential of these OA-nanovesicles loaded with LY500307. Indeed, as these vehicles can combine the anticancer drug-associated specificity with reduced side effects, OA-based nanoemulsions could represent a possible future strategy to fight cancer, including melanoma.

Produzione scientifica

11573/1686117 - 2021 - The sex-related interplay between tme and cancer: on the critical role of estrogen, micrornas and autophagy
Matarrese, P.; Mattia, G.; Pagano, M. T.; Pontecorvi, G.; Ortona, E.; Malorni, W.; Care, A. - 01g Articolo di rassegna (Review)
rivista: CANCERS (Basel: MDPI) pp. 1-22 - issn: 2072-6694 - wos: WOS:000671288400001 (12) - scopus: 2-s2.0-85108879080 (14)

11573/1674380 - 2021 - Synergy between vitamin D and sex hormones in respiratory functionality of patients affected by COVID-19
Peruzzu, D.; Pagano, M. T.; Pierdominici, M.; Ruggieri, A.; Antinori, A.; D'offizi, G.; Petrosillo, N.; Palmieri, F.; Piselli, P.; Boumis, E.; Notari, S.; Nicastri, E.; Agrati, C.; Ippolito, G.; Gagliardi, M. C.; Capobianchi, M. R.; Ortona, E.; Anticoli, S.; Bellenghi, M.; Bordoni, V.; Camici1, M.; Casetti, R.; Cerva, C.; Chinello, P.; Imini, E.; Donno, D. R.; Dupuis, M. L.; Faraglia, F.; Fecchi, K.; Gagliardini, R.; Grassi, G.; Di Lorenzo, R.; Macchione, M.; Maffongelli, G.; Mattia, G.; Milozzi, E.; Pontecorvi, G.; Puglisi, R.; Sacchi, A.; Tartaglia, E.; Vita, S. - 01a Articolo in rivista
rivista: FRONTIERS IN PHARMACOLOGY (Lausanne: Frontiers Media S.A., 2010-) pp. 1-5 - issn: 1663-9812 - wos: WOS:000654963300001 (6) - scopus: 2-s2.0-85107142427 (5)

11573/1661541 - 2021 - A Sex Perspective in Neurodegenerative Diseases: microRNAs as Possible Peripheral Biomarkers
Piscopo, Paola; Bellenghi, Maria; Manzini, Valeria; Crestini, Alessio; Pontecorvi, Giada; Corbo, Massimo; Ortona, Elena; Carè, Alessandra; Confaloni, Annamaria - 01g Articolo di rassegna (Review)
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. 4423- - issn: 1422-0067 - wos: WOS:000650366000001 (15) - scopus: 2-s2.0-85104545640 (15)

11573/1433804 - 2020 - Sex and gender disparities in melanoma
Bellenghi, Maria; Puglisi, Rossella; Pontecorvi, Giada; De Feo, Alessandra; Carè, Alessandra; Mattia, Gianfranco - 01g Articolo di rassegna (Review)
rivista: CANCERS (Basel: MDPI) pp. 1-24 - issn: 2072-6694 - wos: WOS:000554140200001 (73) - scopus: 2-s2.0-85088718060 (81)

11573/1364196 - 2020 - Tumor-derived extracellular vesicles and microRNAs: functional roles, diagnostic, prognostic and therapeutic options
Pontecorvi, G.; Bellenghi, M.; Puglisi, R.; Care, A.; Mattia, G. - 01g Articolo di rassegna (Review)
rivista: CYTOKINE & GROWTH FACTOR REVIEWS (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. 75-83 - issn: 1359-6101 - wos: WOS:000514566400010 (29) - scopus: 2-s2.0-85077545348 (28)

11573/1433802 - 2020 - microRNAs as new possible actors in gender disparities of Covid-19 pandemic
Pontecorvi, Giada; Bellenghi, Maria; Ortona, Elena; Carè, Alessandra - 01m Editorial/Introduzione in rivista
rivista: ACTA PHYSIOLOGICA (Oxford : Blackwell Publishing) pp. - - issn: 1748-1716 - wos: WOS:000553393500001 (16) - scopus: 2-s2.0-85088808277 (16)

11573/1420367 - 2020 - Circulating miR34a levels as a potential biomarker in the follow-up of Ewing sarcoma
Sciandra, M.; De Feo, A.; Parra, A.; Landuzzi, L.; Lollini, P. -L.; Manara, M. C.; Mattia, G.; Pontecorvi, G.; Baricordi, C.; Guerzoni, C.; Bazzocchi, A.; Longhi, A.; Scotlandi, K. - 01a Articolo in rivista
rivista: JOURNAL OF CELL COMMUNICATION AND SIGNALING (Netherlands: Springer Netherlands) pp. 335-347 - issn: 1873-9601 - wos: WOS:000538228800001 (7) - scopus: 2-s2.0-85086124869 (9)

11573/1337972 - 2019 - On exosome functional role in cancer. miR-494 complex regulation in melanoma cells and corresponding exosomes
Bellenghi, Maria; Pontecorvi, Giada; Carè, Alessandra - 01b Commento, Erratum, Replica e simili
rivista: TRANSLATIONAL CANCER RESEARCH (Hong Kong: Pioneer Bioscience Publishing Company) pp. 725-728 - issn: 2219-6803 - wos: WOS:000473292100004 (2) - scopus: 2-s2.0-85068181977 (3)

11573/1337953 - 2019 - Autoantibodies specific to ERa are involved in tamoxifen resistance in hormone receptor positive breast cancer
Maselli, Angela; Parlato, Stefania; Puglisi, Rossella; Raggi, Carla; Spada, Massimo; Macchia, Daniele; Pontecorvi, Giada; Iessi, Elisabetta; Teresa Pagano, Maria; Cirulli, Francesca; Gabriele, Lucia; Carè, Alessandra; Vici, Patrizia; Pizzuti, Laura; Barba, Maddalena; Matarrese, Paola; Pierdominici, Marina; Ortona, Elena - 01a Articolo in rivista
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. 1-14 - issn: 2073-4409 - wos: WOS:000478902000022 (7) - scopus: 2-s2.0-85089482157 (8)

11573/1338848 - 2019 - NRAS-mutated melanoma cell lines are susceptible to G2/M blockage and cell death activation in response to estrogen treatment
Pontecorvi, G; Puglisi, R; Bellenghi, M; Tirelli, V; Sanchez, M; Carè, A; Mattia, G - 04d Abstract in atti di convegno
congresso: 61st Annual Meeting of the Italian Cancer Society (SIC). Precision Oncology: from myth to reality. (Naples)
libro: Abstract book 61st Annual Meeting of the Italian Cancer Society (SIC). Precision Oncology: from myth to reality. Naples, 6-8 November 2019 - ()

11573/1338856 - 2019 - Role of Estrogen receptor β against melanoma progression
Pontecorvi, G; Puglisi, R; Bellenghi, M; Tirelli, V; Sanchez, M; Carè, A; Mattia, G - 04f Poster
congresso: 4th Annual Meeting of Alliance Against Cancer (ACC). New technologies and strategies to fight cancer. Rome, 20-22 November 2019. (Rome)
libro: 4th Annual Meeting of Alliance Against Cancer (ACC). New technologies and strategies to fight cancer. Rome, 20-22 November 2019 - ()

11573/1175916 - 2018 - SCD5 restored expression favors differentiation and epithelial-mesenchymal reversion in advanced melanoma
Puglisi, Rossella; Bellenghi, Maria; Pontecorvi, Giada; Alessandro, Gulino; Marina, Petrini; Federica, Felicetti; Lisabianca, Bottero; Gianfranco, Mattia; Alessandra, Carè - 01a Articolo in rivista
rivista: ONCOTARGET (Albany, N.Y. : Impact Journals) pp. 7567-7581 - issn: 1949-2553 - wos: (0) - scopus: 2-s2.0-85040983893 (16)

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