FRANCESCA MOLINARI

Dottoressa di ricerca

ciclo: XXXIII



Titolo della tesi: Sirt5 inhibition induces brown fat-like phenotype in 3T3L1 preadipocytes

Brown adipose tissue (BAT) activity plays a key role in regulating systemic energy. Activation of BAT results in increased energy expenditure, making this tissue an attractive pharmacological target for therapies against obesity and type 2 diabetes. Sirtuin 5 (SIRT5) affects BAT function by regulating adipogenic transcription factors expression and mitochondrial respiration. Here we treated 3T3-L1 preadipocytes and mouse primary preadipocyte cultures with the SIRT5 inhibitor MC3482 and investigated the effects of this compound on adipose differentiation and function. Administration of MC3482 during the early stages of differentiation promoted expression of brown adipocyte and mitochondrial biogenesis markers. Upon treatment with MC3482, 3T3-L1 adipocytes showed increased activation of AMP-activated protein kinase (AMPK), which potentially stimulates brown adipocyte differentiation. Such effect was paralleled by an increase in autophagic/mitophagic flux and a reduction in lipid droplet size mediated by a higher lipolityc rate. Of note, MC3482 increased expression and activity of adipose triglyceride lipase, without modulating hormone-sensitive lipase. Our findings reveal that SIRT5 inhibition stimulates brown adipogenesis in vitro and may be considered as a strategy to stimulate BAT and counteract obesity.

Produzione scientifica

  • 11573/1120702 - 2016 - Animal models of cardiac cachexia (01a Articolo in rivista)
    MOLINARI, FRANCESCA
  • 11573/1120726 - 2009 - Peripheral blood mononuclear cells from mild cognitive impairment patients show deregulation of Bax and Sod1 mRNAs (01a Articolo in rivista)
    MOLINARI, FRANCESCA; STOCCHI, FABRIZIO
  • 11573/1120719 - 2012 - Molecular control of neuromuscular junction development (01a Articolo in rivista)
    MOLINARI, FRANCESCA
  • 11573/1120693 - 2015 - Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth (01a Articolo in rivista)
    MOLINARI, FRANCESCA
  • 11573/1240179 - 2012 - Effects of a short-term exercise training on serum factors involved in ventricular remodelling in chronic heart failure patients (01a Articolo in rivista)
    MOLINARI, FRANCESCA
  • 11573/1120711 - 2013 - Severity of left ventricular dysfunction in heart failure patients affects the degree of serum-induced cardiomyocyte apoptosis. Importance of inflammatory response and metabolism (01a Articolo in rivista)
    MOLINARI, FRANCESCA
  • 11573/1346896 - 2019 - Mitophagy and oxidative stress in cancer and aging: Focus on sirtuins and nanomaterials (01g Articolo di rassegna (Review))
    VERNUCCI, ENZA; TOMINO, CARLO; MOLINARI, FRANCESCA; LIMONGI, DOLORES; AVENTAGGIATO, MICHELE; SANSONE, LUIGI; TAFANI, MARCO; RUSSO, MATTEO ANTONIO

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma