FRANCESCA GIULIMONDI

Dottoressa di ricerca

ciclo: XXXIII



Titolo della tesi: The protein corona of lipid nanosystems: from structure to physiological response

In the last few years, many efforts have been made in the research fields of nanoparticle-based therapeutics approaches for drug and gene delivery. In this context, liposomal platforms represent very promising systems. Indeed, they are biocompatible, have structural features that allow the encapsulation of molecules with different physical-chemical properties and offer protection to the encapsulated agents from degradative processes. Despite their peculiarities, once injected into the systemic circulation, liposomes acquire a new biological identity as a result of a competition between different biological molecules to adsorb on their surface and form an outer layer, referred to as "protein corona" (PC). As protein adsorption appeared to be inevitable, in this Ph. D. work, we provided a deeper understanding of the dynamics that take place to nanoparticles in complex physiological environments. Firstly, we studied liposome-corona complexes in terms of structural properties, composition and interactions with peripheral blood mononuclear cells (PBMCs) in whole blood. Based on this, we investigate the evolution of liposome-protein complexes as a function of protein concentration. To this end, we employed cationic (CL), neutrally charged (NL) and anionic (AL) liposomes as model systems of gene delivery systems. Then, we investigated and characterized the behaviour of a multicomponent liposomal formulation as a gene delivery system. Taken together, our results confirmed the peculiar key role of the PC in affecting the properties of the nanoparticle-protein complexes. Finally, we presented two PC's unusual interpretations and their implication for future finely target delivery and diagnostic applications. Firstly, we proposed an alternative strategy, i.e. pre-coat nanomaterials with an artificial PC that naturally shuttle to the desired cells upon contact with the bloodstream. All our findings pointed out that prior coating could endow the nanomaterials with a target-specific interaction, specifically by acting on the pre-coating composition it could promote or repress the interaction with the immune system cells. Lastly, we presented some experiments on personalized PC and their implication for diagnostic applications. We proposed a blood test for a new pancreatic cancer diagnostic technology based on the nano-bio-interactions between Graphene Oxide (GO) nanoflakes and blood samples. Globally, we contributed to a detailed knowledge about NP-corona systems for both therapeutic and diagnostic purposes and our findings may help accelerate the clinical translation of NPs from bench to bedside.

Produzione scientifica

  • 11573/1637702 - 2021 - A Proteomic Study on the Personalized Protein Corona of Liposomes. Relevance for Early Diagnosis of Pancreatic DUCTAL Adenocarcinoma and Biomarker Detection (01a Articolo in rivista)
    DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; POZZI, DANIELA; COPPOLA, ALESSANDRO; CAPUTO, DAMIANO; CARACCIOLO, GIULIO
  • 11573/966707 - 2017 - Identification of a novel chalcone derivative that inhibits Notch signaling in T-cell acute lymphoblastic leukemia (01a Articolo in rivista)
    MORI, MATTIA; TOTTONE, LUCA; QUAGLIO, DEBORAH; ZHDANOVSKAYA, NADEZDA; INGALLINA, CINZIA; GHIRGA, FRANCESCA; PERUZZI, GIOVANNA; CRESTONI, MARIA ELISA; GIULIMONDI, FRANCESCA; TALORA, CLAUDIO; BOTTA, BRUNO; SCREPANTI, ISABELLA; PALERMO, ROCCO
  • 11573/1341399 - 2019 - Interplay of protein corona and immune cells controls blood residency of liposomes (01a Articolo in rivista)
    GIULIMONDI, FRANCESCA; DIGIACOMO, LUCA; POZZI, DANIELA; PALCHETTI, SARA; VULPIS, ELISABETTA; CAPRIOTTI, ANNA LAURA; LAGANA', ALDO; MASUELLI, LAURA; SCREPANTI, ISABELLA; ZINGONI, ALESSANDRA; CARACCIOLO, GIULIO
  • 11573/1341407 - 2019 - The biomolecular corona of gold nanoparticles in a controlled microfluidic environment (01a Articolo in rivista)
    DIGIACOMO, LUCA; PALCHETTI, SARA; GIULIMONDI, FRANCESCA; POZZI, DANIELA; ZENEZINI CHIOZZI, RICCARDO; CAPRIOTTI, ANNA LAURA; LAGANA', ALDO; CARACCIOLO, GIULIO
  • 11573/1348816 - 2019 - Effect of molecular crowding on the biological identity of liposomes: an overlooked factor at the bio-nano interface (01a Articolo in rivista)
    DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; CARACCIOLO, GIULIO
  • 11573/1671029 - 2023 - Reproducibility of biomolecular corona experiments: a primer for reliable results (01a Articolo in rivista)
    GIULIMONDI, FRANCESCA; QUAGLIARINI, ERICA; DIGIACOMO, LUCA; RENZI, SERENA; POZZI, DANIELA; CARACCIOLO, GIULIO
  • 11573/1347921 - 2019 - Converting the personalized biomolecular corona of graphene oxide nanoflakes into a high-throughput diagnostic test for early cancer detection (01a Articolo in rivista)
    PAPI, MASSIMILIANO; DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; PALCHETTI, SARA; CIASCA, GABRIELE; CAPRIOTTI, ANNA LAURA; LAGANA', ALDO; POZZI, DANIELA; CARACCIOLO, GIULIO
  • 11573/1425805 - 2020 - A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression (01a Articolo in rivista)
    PALCHETTI, SARA; DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; POZZI, DANIELA; PERUZZI, GIOVANNA; CARDARELLI, FRANCESCO; CARACCIOLO, GIULIO
  • 11573/1543169 - 2021 - Efficient pancreatic cancer detection through personalized protein corona of gold nanoparticles (01a Articolo in rivista)
    DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; PALCHETTI, SARA; POZZI, DANIELA; CARACCIOLO, GIULIO
  • 11573/1553490 - 2021 - The possible role of sex as an important factor in development and administration of lipid nanomedicine-based COVID-19 vaccine (01a Articolo in rivista)
    VULPIS, ELISABETTA; GIULIMONDI, FRANCESCA; DIGIACOMO, LUCA; ZINGONI, ALESSANDRA; CARACCIOLO, GIULIO
  • 11573/1569003 - 2021 - Optimal centrifugal isolating of liposome-protein complexes from human plasma (01a Articolo in rivista)
    DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; CAPRIOTTI, ANNA LAURA; PIOVESANA, SUSY; MONTONE, CARMELA MARIA; LAGANA', ALDO; POZZI, DANIELA; CARACCIOLO, GIULIO
  • 11573/1615334 - 2022 - Opsonin-deficient nucleoproteic corona Endows unPEGylated liposomes with stealth properties in vivo (01a Articolo in rivista)
    GIULIMONDI, FRANCESCA; VULPIS, ELISABETTA; DIGIACOMO, LUCA; GIULI, MARIA VALERIA; MANCUSI, ANGELICA; CAPRIOTTI, ANNA LAURA; LAGANA', ALDO; CERRATO, ANDREA; ZENEZINI CHIOZZI, RICCARDO; NICOLETTI, CARMINE; MASUELLI, LAURA; SCREPANTI, ISABELLA; POZZI, DANIELA; ZINGONI, ALESSANDRA; CHECQUOLO, SAULA; CARACCIOLO, GIULIO
  • 11573/1616584 - 2021 - Microfluidic formulation of DNA-loaded multicomponent lipid nanoparticles for gene delivery (01a Articolo in rivista)
    QUAGLIARINI, ERICA; RENZI, SERENA; DIGIACOMO, LUCA; GIULIMONDI, FRANCESCA; TASSINARI, VALENTINA; MASUELLI, LAURA; POZZI, DANIELA; CARACCIOLO, GIULIO
  • 11573/1656112 - 2022 - In vitro and ex vivo nano-enabled immunomodulation by the protein corona (01a Articolo in rivista)
    GIULIMONDI, FRANCESCA; DIGIACOMO, LUCA; VULPIS, ELISABETTA; LOCONTE, LUISA; POZZI, DANIELA; ZINGONI, ALESSANDRA; CARACCIOLO, GIULIO
  • 11573/1659102 - 2022 - The protein corona reduces the anticancer effect of graphene oxide in HER-2-positive cancer cells (01a Articolo in rivista)
    QUAGLIARINI, ERICA; XIAO, SIYAO; GIULIMONDI, FRANCESCA; RENZI, SERENA; DIGIACOMO, LUCA; CARACCIOLO, GIULIO; POZZI, DANIELA
  • 11573/1543148 - 2021 - Inhibiting the growth of 3D brain cancer models with bio-coronated liposomal temozolomide (01a Articolo in rivista)
    GIULIMONDI, FRANCESCA; DIGIACOMO, LUCA; QUAGLIARINI, ERICA; POZZI, DANIELA; PAPI, MASSIMILIANO; CARACCIOLO, GIULIO
  • 11573/1498423 - 2016 - NOTCH inhibitors for use in the treatment of T-cell acute lymphoblastic leukemia (05a Brevetto)
    BOTTA, BRUNO; SCREPANTI, ISABELLA; INGALLINA, CINZIA; GIULIMONDI, FRANCESCA; QUAGLIO, DEBORAH; PALERMO, ROCCO

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