Research: New insights into the role of the HECT-E3 Ligase SMURFs in the modulation of the Hedgehog Pathway
WORK ADDRESS: Laboratory of Molecular Oncology (Department of Molecular Medicine), Viale Regina Elena 291, 00161 Rome, Italy
SUPERVISOR: Prof. Giuseppe Giannini
CO-SUPERVISOR: Prof. Enrico De Smaele
PREVIOUS RESEARCH EXPERIENCES:
October 2019 – October 2020: Undergraduate Student in Laboratory of Molecular Medicine, Dep. Molecular Oncology, University of Rome “La Sapienza”. Tutor: Prof. Enrico De Smaele. Role of KCASH2 protein in the regulation of Hedgehog signaling pathway and characterization of the cerebellar phenotype of the KCASH2-KO mouse.
October 2017 – October 2018: Undergraduate Student in Laboratory of General Physiology and Anthropology Pharmacy, Dep. Biology and Biotechnology “Charles Darwin”, University of Rome “La Sapienza”. Tutor: Prof. Giuseppe Lupo. Role of Dbx2 gene in neural aging of neural stem cells.
TRAINING:
November 2020 - In corso: PhD in Molecular Medicine at the University of Rome “La Sapienza”.
October 2020 – Master’s degree in Neurobiology at the University of Rome “La Sapienza”. Score: 110/110 e lode. Thesis title: “Characterization of the cerebellar phenotype of the KCASH2-KO mouse”. Tutor: Prof. Enrico De Smaele
October 2018 – Bachelor’s degree in Biological Science at the University of Rome “La Sapienza”. Score: 110/110 e lode. Thesis title: “Inducible overexpression of the Dbx2 gene in neural stem cells”. Tutor: Prof. Giuseppe Lupo.
SKILLS:
- Cell cultures techniques
Commercial cell cultures and primary cultures
Clonal cultures
Cultures of stem cells derived from the dentate gyrus
Adhesion neural stem cell cultures
Cultures of neural stem cells in suspension (neurospheres)
- Molecular biology techniques
Co-immunoprecipitation assays
Ubiquitination essays
Proliferation assays by incorporation of BrdU
DNA extraction, cloning, PCR
Transfection techniques
Protein lysate extraction and Western Blot
Immunofluorescence and immunohistochemistry assays
Beta-galactosidase assay
CRISPR/CAS9 technology
Immunoprecipitation of chromatin
Gene expression measurement: RNA extraction and RT-qPCR
Transcriptional activation controlled by tetracycline
- Animal experimentation
Tail grip manipulation
Sexing
Basic handling
Marking systems (marker pen, ear punch, earring)
Urine and feces collection
Gavage
Blood sampling (mandibular plexus, tail)
Inocula (intraperitoneal, subcutaneous, intravenous, intramuscular)
Necropsy
- Language skills: English B1
- Informatic Skills: Basic Computer Skills: Operating Systems (Good), Programming (Fair), Word Processing (Good), Spreadsheets (Good), Database Managers (Fair), Internet Browsing (Good), Website Creation (Good ),
Multimedia (sound, images, video) (Good). Programming Languages: (C++, Javascript)