EMILY SCHIFANO

Dottoressa di ricerca

ciclo: XXXII



Titolo della tesi: The host pathogen interactions in the Caenorhabditis elegans animal model: the role of glycosylation and a tool to screen for probiotic bacteria

In this project, the role of the pmr 1 gene in the glycosylation process and i n the host microrganism interaction was analyzed in C. elegans model system. Glycosylation produces a large number of glycans, polysaccharides whose different morphology reflects the multiplicity of biological processes in which they are involved. The emer ging role of glycans in the innate and acquired immunity of vertebrates makes them a target for the study of the molecular mechanisms underlying the host pathogen interaction, in order to develop new therapeutic approaches. The Ca 2+ ATPase, encoded by the pmr 1 gene, appears to be involved in alterations of glycosylation mechanisms, as demonstrated by studies on the homologous PMR 1 protein of Saccharomyces cerevisiae and Kluveromyces lactis . In this work, pmr 1 mutant nematodes, obtained through RNA interf erence, were exposed to different pathogenic microorganisms, showing an increased survival capacity compared to wild type individuals, following infection by the Gram positive Staphylococcus aureus and Enterococcus faecalis pathogens. This increased resist ance seems to be related to defects in the oligosaccharide structure of glycoproteins of the cell surface caused by their altered glycosylation. The lack of regular recognition of C. elegans surface glycoproteins by the two pathogens could, in fact, determ ine the difficult adhesion of bacteria to the nematode and the reduction of their pathogenicity. Furthermore, pmr 1 worms showed a different innate immune and oxidative stress responses, as well as an alteration of the glycocalyx in their gut. Since glycos ylation is also involved in beneficial role of host microbiota against pathogens, nematodes were used to preselect probiotic bacteria, whose efficacy will be evaluate after administration on pmr 1 mutants, to measure the response of worms to pathogen infec tion. Therefore, this study represents a first step in understanding the role of PMR 1 protein in the host microrganism interaction in C. elegans

Produzione scientifica

11573/1701966 - 2024 - Bacterial nanocellulose hydrogel for the green cleaning of copper stains from marble
Sonaglia, Erica; Schifano, Emily; Sharbaf, Mohammad; Uccelletti, Daniela; Felici, Anna Candida; Santarelli, Maria Laura - 01a Articolo in rivista
rivista: GELS (Basel : MDPI) pp. - - issn: 2310-2861 - wos: WOS:001170060200001 (3) - scopus: (0)

11573/1678003 - 2023 - ZnO Nanorods Create a Hypoxic State with Induction of HIF-1 and EPAS1, Autophagy, and Mitophagy in Cancer and Non-Cancer Cells
Aventaggiato, Michele; Preziosi, Adele; Cheraghi Bidsorkhi, Hossein; Schifano, Emily; Vespa, Simone; Mardente, Stefania; Zicari, Alessandra; Uccelletti, Daniela; Mancini, Patrizia; Lotti, Lavinia Vittoria; Sarto, Maria Sabrina; Tafani, Marco - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:000977678900001 (4) - scopus: 2-s2.0-85157966007 (4)

11573/1692790 - 2023 - Electrospinning technology to upscale the production of low-cost and high-filtering functionalized polymer-based nanofibers providing photocatalytic activity for bacterial inactivation in advanced face mask
Cimini, Adriano; Borgioni, Alessia; Mancini, Chiara; Proietti, Anacleto; Buccini, Luca; Stornelli, Eleonora; Schifano, Emily; Dinarelli, Simone; Mura, Francesco; Passeri, Daniele; Imperi, Enrico; Rinaldi, Teresa; Picano, Alfredo; Rossi, Marco - 04d Abstract in atti di convegno
congresso: NANOCON 2023 (Brno (Repubblica Ceca))
libro: Nanocon 2023 - ()

11573/1692785 - 2023 - Upscaling of Electrospinning Technology and the Application of Functionalized PVDF-HFP@TiO2 Electrospun Nanofibers for the Rapid Photocatalytic Deactivation of Bacteria on Advanced Face Masks
Cimini, Adriano; Borgioni, Alessia; Passarini, Elena; Mancini, Chiara; Proietti, Anacleto; Buccini, Luca; Stornelli, Eleonora; Schifano, Emily; Dinarelli, Simone; Mura, Francesco; Sergi, Claudia; Bavasso, Irene; Cortese, Barbara; Passeri, Daniele; Imperi, Enrico; Rinaldi, Teresa; Picano, Alfredo; Rossi, Marco - 01a Articolo in rivista
rivista: POLYMERS (Basel : Molecular Diversity Preservation International) pp. - - issn: 2073-4360 - wos: WOS:001117505800001 (1) - scopus: 2-s2.0-85179129565 (1)

11573/1679573 - 2023 - In vitro probiotic properties and in vivo anti-ageing effects of Lactoplantibacillus plantarum PFA2018AU strain isolated from carrots on Caenorhabditis elegans
Pompa, Laura; Montanari, Arianna; Tomassini, Alberta; Bianchi, Michele Maria; Aureli, Walter; Miccheli, Alfredo; Uccelletti, Daniela; Schifano, Emily - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000979504400001 (2) - scopus: 2-s2.0-85156214835 (2)

11573/1675840 - 2023 - Plasma-etched vertically aligned CNTs with enhanced antibacterial power
Schifano, Emily; Cavoto, Gianluca; Pandolfi, Francesco; Pettinari, Giorgio; Apponi, Alice; Ruocco, Alessandro; Uccelletti, Daniela; Rago, Ilaria Carmela - 01a Articolo in rivista
rivista: NANOMATERIALS (Basel : MDPI) pp. - - issn: 2079-4991 - wos: WOS:000959264400001 (5) - scopus: 2-s2.0-85151499707 (7)

11573/1673864 - 2023 - Evaluation of the irradiation treatment effects on ancient parchment samples
Vadrucci, Monia; Cicero, Cristina; Mazzuca, Claudia; Severini, Leonardo; Uccelletti, Daniela; Schifano, Emily; Mercuri, Fulvio; Zammit, Ugo; Orazi, Noemi; D’Amico, Francesco; Parisse, Pietro - 01a Articolo in rivista
rivista: HERITAGE (Basel: MDPI AG, March 2018-) pp. 1308-1324 - issn: 2571-9408 - wos: WOS:000945077500001 (2) - scopus: 2-s2.0-85149223168 (3)

11573/1660144 - 2022 - Silencing of the mitochondrial ribosomal protein L-24 gene activates the oxidative stress response in Caenorhabditis elegans
Ficociello, Graziella; Schifano, Emily; Di Nottia, Michela; Torraco, Alessandra; Carrozzo, Rosalba; Uccelletti, Daniela; Montanari, Arianna - 01a Articolo in rivista
rivista: BIOCHIMICA ET BIOPHYSICA ACTA. GENERAL SUBJECTS (Amsterdam: Elsevier BV) pp. - - issn: 1872-8006 - wos: WOS:000880164500001 (2) - scopus: 2-s2.0-85140320220 (2)

11573/1664526 - 2022 - Neem oil or almond oil nanoemulsions for vitamin E Delivery: from structural evaluation to in vivo assessment of antioxidant and anti-inflammatory activity
Rinaldi, Federica; Hanieh, Patrizia Nadia; Maurizi, Linda; Longhi, Catia; Uccelletti, Daniela; Schifano, Emily; Del Favero, Elena; Cantù, Laura; Ricci, Caterina; Ammendolia, Maria Grazia; Paolino, Donatella; Froiio, Francesca; Marianecci, Carlotta; Carafa, Maria - 01a Articolo in rivista
rivista: INTERNATIONAL JOURNAL OF NANOMEDICINE (Auckland, N.Z. : Dove Medical Press, c2006-) pp. 6447-6465 - issn: 1176-9114 - wos: WOS:000902038700001 (1) - scopus: 2-s2.0-85144284525 (3)

11573/1661960 - 2022 - 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans
Schifano, Emily; Conta, Giorgia; Preziosi, Adele; Ferrante, Carino; Batignani, Giovanni; Mancini, Patrizia; Tomassini, Alberta; Sciubba, Fabio; Scopigno, Tullio; Uccelletti, Daniela; Miccheli, Alfredo - 01a Articolo in rivista
rivista: FRONTIERS IN MOLECULAR BIOSCIENCES (Lausanne : Frontiers Media S.A., 2014-) pp. 1-18 - issn: 2296-889X - wos: WOS:000897896300001 (4) - scopus: 2-s2.0-85144068548 (4)

11573/1657547 - 2022 - Semantics of dairy fermented foods: a microbiologist’s perspective
Vitali, Francesco; Zinno, Paola; Schifano, Emily; Gori, Agnese; Costa, Ana; De Filippo, Carlotta; Koroušić Seljak, Barbara; Panov, Panče; Devirgiliis, Chiara; Cavalieri, Duccio - 01a Articolo in rivista
rivista: FOODS (Basel: MDPI) pp. - - issn: 2304-8158 - wos: WOS:000825579900001 (2) - scopus: 2-s2.0-85133644910 (2)

11573/1660145 - 2022 - FDF-DB: a database of traditional fermented dairy foods and their associated microbiota
Zinno, Paola; Maria Calabrese, Francesco; Schifano, Emily; Sorino, Paolo; Di Cagno, Raffaella; Gobbetti, Marco; Parente, Eugenio; De Angelis, Maria; Devirgiliis, Chiara - 01a Articolo in rivista
rivista: NUTRIENTS (Basel, Switzerland: Molecular Diversity Preservation International (MDPI)) pp. - - issn: 2072-6643 - wos: WOS:000881711500001 (3) - scopus: 2-s2.0-85141746941 (3)

11573/1584711 - 2021 - Colonization ability and impact on human gut microbiota of foodborne microbes from traditional or probiotic-added fermented foods: a systematic review
Roselli, Marianna; Natella, Fausta; Zinno, Paola; Guantario, Barbara; Canali, Raffaella; Schifano, Emily; De Angelis, Maria; Nikoloudaki, Olga; Gobbetti, Marco; Perozzi, Giuditta; Devirgiliis, Chiara - 01a Articolo in rivista
rivista: FRONTIERS IN NUTRITION (Lausanne : Frontiers Media S.A., 2014-) pp. - - issn: 2296-861X - wos: WOS:001026951400001 (29) - scopus: 2-s2.0-85112681777 (31)

11573/1584705 - 2021 - Leuconostoc mesenteroides strains isolated from carrots show probiotic features
Schifano, Emily; Tomassini, Alberta; Preziosi, Adele; Montes, Jorge; Aureli, Walter; Mancini, Patrizia; Miccheli, Alfredo; Uccelletti, Daniela - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000728061500001 (10) - scopus: 2-s2.0-85118382094 (11)

11573/1352544 - 2020 - A combined proteomics, metabolomics and in vivo analysis approach for the characterization of probiotics in a large-scale production
Bianchi, Laura; Laghi, Luca; Correani, Virginia; Schifano, Emily; Landi, Claudia; Uccelletti, Daniela; Mattei, Benedetta - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. - - issn: 2218-273X - wos: WOS:000514863200152 (12) - scopus: 2-s2.0-85078309336 (18)

11573/1364989 - 2020 - Nanopore sequencing and bioinformatics for rapidly identifying cultural heritage spoilage microorganisms
Grottoli, Alessandro; Beccaccioli, Marzia; Emma, Zoppis; Rosita Silvana Fratini, ; Schifano, Emily; Santarelli, Maria Laura; Uccelletti, Daniela; Reverberi, Massimo - 01a Articolo in rivista
rivista: FRONTIERS IN MATERIALS (Lausanne : Frontiers Media S.A., 2013-) pp. 1-11 - issn: 2296-8016 - wos: WOS:000525090100001 (21) - scopus: 2-s2.0-85081243815 (21)

11573/1362762 - 2020 - Antibacterial effect of zinc oxide-based nanomaterials on environmental biodeteriogens affecting historical buildings
Schifano, Emily; Cavallini, Domenico; De Bellis, Giovanni; Bracciale, Maria Paola; Felici, Anna Candida; Santarelli, Maria Laura; Sarto, Maria Sabrina; Uccelletti, Daniela - 01a Articolo in rivista
rivista: NANOMATERIALS (Basel : MDPI) pp. 1-14 - issn: 2079-4991 - wos: WOS:000522456300154 (22) - scopus: 2-s2.0-85079633769 (30)

11573/1447747 - 2020 - In vitro and in vivo lipidomics as a tool for probiotics evaluation
Schifano, Emily; Cicalini, Ilaria; Pieragostino, Damiana; Heipieper, Hermann J.; Del Boccio, Piero; Uccelletti, Daniela - 01a Articolo in rivista
rivista: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (Germany: Springer Verlag Germany:Tiergartenstrasse) pp. 8937-8948 - issn: 0175-7598 - wos: WOS:000565167000005 (7) - scopus: 2-s2.0-85090058606 (7)

11573/1361102 - 2020 - Effects of the ionizing radiation disinfection treatment on historical leather
Vadrucci1, Monia; De Bellis, Giovanni; Mazzuca, Claudia; Mercuri, Fulvio; Borgognoni, Fabio; Schifano, Emily; Uccelletti, Daniela; Cicero, Cristina - 01a Articolo in rivista
rivista: FRONTIERS IN MATERIALS (Lausanne : Frontiers Media S.A., 2013-) pp. 1-9 - issn: 2296-8016 - wos: WOS:000525086700001 (18) - scopus: 2-s2.0-85082596215 (20)

11573/1319909 - 2019 - Caenorhabditis elegans and probiotics interactions from a prolongevity perspective
Marianna, Roselli; Schifano, Emily; Barbara, Guantario; Paola, Zinno; Uccelletti, Daniela; Chiara, Devirgiliis - 01g Articolo di rassegna (Review)
rivista: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (Basel: MDPI Center) pp. - - issn: 1422-0067 - wos: WOS:000498822800052 (38) - scopus: 2-s2.0-85074241139 (39)

11573/1331813 - 2019 - Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways alterations
Schifano, Emily; Ficociello, Graziella; Vespa, Simone; Ghosh, Salil; Cipollo John, F; Talora, Claudio; Lotti, Lavinia Vittoria; Mancini, Patrizia; Uccelletti, Daniela. - 01a Articolo in rivista
rivista: VIRULENCE (Austin, Tex.: Landes Bioscience) pp. 1013-1025 - issn: 2150-5594 - wos: WOS:000501036200001 (5) - scopus: 2-s2.0-85075608232 (5)

11573/1263277 - 2019 - The foodborne strain Lactobacillus fermentum MBC2 triggers pept-1-dependent pro-longevity effects in Caenorhabditis elegans
Schifano, Emily; Paola, Zinno; Barbara, Guantario; Marianna, Roselli; Marcoccia, Sante; Chiara, Devirgiliis; Uccelletti, Daniela - 01a Articolo in rivista
rivista: MICROORGANISMS (Basel : MDPI) pp. - - issn: 2076-2607 - wos: WOS:000460169900028 (35) - scopus: 2-s2.0-85074352331 (36)

11573/1112597 - 2018 - In vitro and in vivo selection of potentially probiotic lactobacilli from Nocellara del Belice table olives
Guantario, B; Zinno, P; Schifano, E; Roselli, M; Perozzi, G; Palleschi, C; Uccelletti, D; Devirgiliis, C. - 01a Articolo in rivista
rivista: FRONTIERS IN MICROBIOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. 1-15 - issn: 1664-302X - wos: WOS:000428533800001 (37) - scopus: 2-s2.0-85044849881 (43)

11573/1184017 - 2018 - Virulence behavior of uropathogenic Escherichia coli strains in the host model Caenorhabditis elegans
Schifano, Emily; Marazzato, Massimiliano; Ammendolia, Maria Grazia; Zanni, Elena; Ricci, Marta; Comanducci, Antonella; Goldoni, Paola; Conte, Maria Pia; Uccelletti, Daniela; Longhi, Catia - 01a Articolo in rivista
rivista: MICROBIOLOGYOPEN (Oxford : Wiley-Blackwell) pp. 1-10 - issn: 2045-8827 - wos: WOS:000471281900023 (13) - scopus: 2-s2.0-85055886386 (13)

11573/984888 - 2017 - Combination of metabolomic and proteomic analysis revealed different features among Lactobacillus delbrueckii subspecies bulgaricus and lactis strains while in vivo testing in the model organism Caenorhabditis elegans highlighted probiotic properties
Zanni, Elena; Schifano, Emily; Motta, Sara; Sciubba, Fabio; Palleschi, Claudio; Mauri, Pierluigi; Perozzi, Giuditta; Uccelletti, Daniela; Devirgiliis, Chiara; Miccheli, Alfredo - 01a Articolo in rivista
rivista: FRONTIERS IN MICROBIOLOGY (Lausanne : Frontiers Research Foundation, 2010-) pp. 1-12 - issn: 1664-302X - wos: WOS:000404329300001 (25) - scopus: 2-s2.0-85021403629 (25)

11573/780800 - 2015 - Impact of a complex food microbiota on energy metabolism in the model organism Caenorhabditis elegans
Zanni, Elena; C., Laudenzi; Schifano, Emily; Palleschi, Claudio; G., Perozzi; Uccelletti, Daniela; C., Devirgiliis - 01a Articolo in rivista
rivista: BIOMED RESEARCH INTERNATIONAL ([London]: [Hindawi] Cairo: Hindawi Publishing Corporation) pp. 1-12 - issn: 2314-6133 - wos: WOS:000353821100001 (33) - scopus: 2-s2.0-84929353324 (33)

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