EMILY SCHIFANO

Dottoressa di ricerca

ciclo: XXXII



Titolo della tesi: The host pathogen interactions in the Caenorhabditis elegans animal model: the role of glycosylation and a tool to screen for probiotic bacteria

In this project, the role of the pmr 1 gene in the glycosylation process and i n the host microrganism interaction was analyzed in C. elegans model system. Glycosylation produces a large number of glycans, polysaccharides whose different morphology reflects the multiplicity of biological processes in which they are involved. The emer ging role of glycans in the innate and acquired immunity of vertebrates makes them a target for the study of the molecular mechanisms underlying the host pathogen interaction, in order to develop new therapeutic approaches. The Ca 2+ ATPase, encoded by the pmr 1 gene, appears to be involved in alterations of glycosylation mechanisms, as demonstrated by studies on the homologous PMR 1 protein of Saccharomyces cerevisiae and Kluveromyces lactis . In this work, pmr 1 mutant nematodes, obtained through RNA interf erence, were exposed to different pathogenic microorganisms, showing an increased survival capacity compared to wild type individuals, following infection by the Gram positive Staphylococcus aureus and Enterococcus faecalis pathogens. This increased resist ance seems to be related to defects in the oligosaccharide structure of glycoproteins of the cell surface caused by their altered glycosylation. The lack of regular recognition of C. elegans surface glycoproteins by the two pathogens could, in fact, determ ine the difficult adhesion of bacteria to the nematode and the reduction of their pathogenicity. Furthermore, pmr 1 worms showed a different innate immune and oxidative stress responses, as well as an alteration of the glycocalyx in their gut. Since glycos ylation is also involved in beneficial role of host microbiota against pathogens, nematodes were used to preselect probiotic bacteria, whose efficacy will be evaluate after administration on pmr 1 mutants, to measure the response of worms to pathogen infec tion. Therefore, this study represents a first step in understanding the role of PMR 1 protein in the host microrganism interaction in C. elegans

Produzione scientifica

  • 11573/780800 - 2015 - Impact of a complex food microbiota on energy metabolism in the model organism Caenorhabditis elegans (01a Articolo in rivista)
    ZANNI, ELENA; SCHIFANO, EMILY; PALLESCHI, CLAUDIO; UCCELLETTI, DANIELA
  • 11573/984888 - 2017 - Combination of metabolomic and proteomic analysis revealed different features among Lactobacillus delbrueckii subspecies bulgaricus and lactis strains while in vivo testing in the model organism Caenorhabditis elegans highlighted probiotic properties (01a Articolo in rivista)
    ZANNI, ELENA; SCHIFANO, EMILY; SCIUBBA, FABIO; PALLESCHI, CLAUDIO; PEROZZI, GIUDITTA; UCCELLETTI, DANIELA; MICCHELI, ALFREDO
  • 11573/1675840 - 2023 - Plasma-etched vertically aligned CNTs with enhanced antibacterial power (01a Articolo in rivista)
    SCHIFANO, EMILY; CAVOTO, GIANLUCA; UCCELLETTI, DANIELA; RAGO, ILARIA CARMELA
  • 11573/1184017 - 2018 - Virulence behavior of uropathogenic Escherichia coli strains in the host model Caenorhabditis elegans (01a Articolo in rivista)
    SCHIFANO, EMILY; MARAZZATO, MASSIMILIANO; AMMENDOLIA, MARIA GRAZIA; ZANNI, ELENA; COMANDUCCI, ANTONELLA; GOLDONI, PAOLA; CONTE, MARIA PIA; UCCELLETTI, DANIELA; LONGHI, CATIA
  • 11573/1263277 - 2019 - The foodborne strain Lactobacillus fermentum MBC2 triggers pept-1-dependent pro-longevity effects in Caenorhabditis elegans (01a Articolo in rivista)
    SCHIFANO, EMILY; MARCOCCIA, SANTE; UCCELLETTI, DANIELA
  • 11573/1679573 - 2023 - In vitro probiotic properties and in vivo anti-ageing effects of Lactoplantibacillus plantarum PFA2018AU strain isolated from carrots on Caenorhabditis elegans (01a Articolo in rivista)
    POMPA, LAURA; MONTANARI, ARIANNA; TOMASSINI, ALBERTA; BIANCHI, MICHELE MARIA; MICCHELI, ALFREDO; UCCELLETTI, DANIELA; SCHIFANO, EMILY
  • 11573/1331813 - 2019 - Pmr-1 gene affects susceptibility of Caenorhabditis elegans to Staphylococcus aureus infection through glycosylation and stress response pathways alterations (01a Articolo in rivista)
    SCHIFANO, EMILY; FICOCIELLO, GRAZIELLA; TALORA, CLAUDIO; LOTTI, LAVINIA VITTORIA; MANCINI, PATRIZIA; UCCELLETTI, DANIELA
  • 11573/1319909 - 2019 - Caenorhabditis elegans and probiotics interactions from a prolongevity perspective (01g Articolo di rassegna (Review))
    SCHIFANO, EMILY; UCCELLETTI, DANIELA
  • 11573/1352544 - 2020 - A combined proteomics, metabolomics and in vivo analysis approach for the characterization of probiotics in a large-scale production (01a Articolo in rivista)
    CORREANI, VIRGINIA; SCHIFANO, EMILY; UCCELLETTI, DANIELA
  • 11573/1372501 - 2020 - The host-pathogen interactions in the Caenorhabditis elegans animal model: the role of glycosylation and a tool to screen for probiotic bacteria (07a Tesi di Dottorato)
    SCHIFANO, EMILY
  • 11573/1361102 - 2020 - Effects of the ionizing radiation disinfection treatment on historical leather (01a Articolo in rivista)
    DE BELLIS, GIOVANNI; SCHIFANO, EMILY; UCCELLETTI, DANIELA
  • 11573/1362762 - 2020 - Antibacterial effect of zinc oxide-based nanomaterials on environmental biodeteriogens affecting historical buildings (01a Articolo in rivista)
    SCHIFANO, EMILY; CAVALLINI, DOMENICO; DE BELLIS, GIOVANNI; BRACCIALE, MARIA PAOLA; FELICI, ANNA CANDIDA; SANTARELLI, MARIA LAURA; SARTO, MARIA SABRINA; UCCELLETTI, DANIELA
  • 11573/1678003 - 2023 - ZnO Nanorods Create a Hypoxic State with Induction of HIF-1 and EPAS1, Autophagy, and Mitophagy in Cancer and Non-Cancer Cells (01a Articolo in rivista)
    AVENTAGGIATO, MICHELE; PREZIOSI, ADELE; CHERAGHI BIDSORKHI, HOSSEIN; SCHIFANO, EMILY; MARDENTE, STEFANIA; ZICARI, ALESSANDRA; UCCELLETTI, DANIELA; MANCINI, PATRIZIA; LOTTI, LAVINIA VITTORIA; SARTO, MARIA SABRINA; TAFANI, MARCO
  • 11573/1364989 - 2020 - Nanopore sequencing and bioinformatics for rapidly identifying cultural heritage spoilage microorganisms (01a Articolo in rivista)
    GROTTOLI, ALESSANDRO; BECCACCIOLI, MARZIA; SCHIFANO, EMILY; SANTARELLI, MARIA LAURA; UCCELLETTI, DANIELA; REVERBERI, MASSIMO
  • 11573/1447747 - 2020 - In vitro and in vivo lipidomics as a tool for probiotics evaluation (01a Articolo in rivista)
    SCHIFANO, EMILY; HEIPIEPER, HERMANN JOSEF; UCCELLETTI, DANIELA
  • 11573/1584705 - 2021 - Leuconostoc mesenteroides strains isolated from carrots show probiotic features (01a Articolo in rivista)
    SCHIFANO, EMILY; TOMASSINI, ALBERTA; PREZIOSI, ADELE; MANCINI, PATRIZIA; MICCHELI, ALFREDO; UCCELLETTI, DANIELA
  • 11573/1657547 - 2022 - Semantics of dairy fermented foods: a microbiologist’s perspective (01a Articolo in rivista)
    VITALI, FRANCESCO; SCHIFANO, EMILY
  • 11573/1660144 - 2022 - Silencing of the mitochondrial ribosomal protein L-24 gene activates the oxidative stress response in Caenorhabditis elegans (01a Articolo in rivista)
    FICOCIELLO, GRAZIELLA; SCHIFANO, EMILY; UCCELLETTI, DANIELA; MONTANARI, ARIANNA
  • 11573/1664526 - 2022 - Neem oil or almond oil nanoemulsions for vitamin E Delivery: from structural evaluation to in vivo assessment of antioxidant and anti-inflammatory activity (01a Articolo in rivista)
    RINALDI, FEDERICA; HANIEH, PATRIZIA NADIA; MAURIZI, LINDA; LONGHI, CATIA; UCCELLETTI, DANIELA; SCHIFANO, EMILY; AMMENDOLIA, MARIA GRAZIA; MARIANECCI, CARLOTTA; CARAFA, MARIA
  • 11573/1661960 - 2022 - 2-hydroxyisobutyric acid (2-HIBA) modulates ageing and fat deposition in Caenorhabditis elegans (01a Articolo in rivista)
    SCHIFANO, EMILY; CONTA, GIORGIA; PREZIOSI, ADELE; FERRANTE, CARINO; BATIGNANI, GIOVANNI; MANCINI, PATRIZIA; TOMASSINI, ALBERTA; SCIUBBA, FABIO; SCOPIGNO, TULLIO; UCCELLETTI, DANIELA; MICCHELI, ALFREDO
  • 11573/1660145 - 2022 - FDF-DB: a database of traditional fermented dairy foods and their associated microbiota (01a Articolo in rivista)
    SCHIFANO, EMILY
  • 11573/1673864 - 2023 - Evaluation of the irradiation treatment effects on ancient parchment samples (01a Articolo in rivista)
    UCCELLETTI, DANIELA; SCHIFANO, EMILY; ORAZI, NOEMI
  • 11573/1112597 - 2018 - In vitro and in vivo selection of potentially probiotic lactobacilli from Nocellara del Belice table olives (01a Articolo in rivista)
    SCHIFANO, EMILY; PEROZZI, GIUDITTA; PALLESCHI, CLAUDIO; UCCELLETTI, DANIELA
  • 11573/1584711 - 2021 - Colonization ability and impact on human gut microbiota of foodborne microbes from traditional or probiotic-added fermented foods: a systematic review (01a Articolo in rivista)
    SCHIFANO, EMILY

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