Research: Targeting SMYD3-mTOR-AMPK metabolic axis during colorectal cancer cells response to DNA damage
WORK ADDRESS:
IRCCS Saverio De Bellis, Castellana Grotte (BA)
SUPERVISOR:
Professore Cristiano Simone
PREVIOUS RESEARCH EXPERIENCE:
06/2023-Today
Fellow at Laboratory of Medical Genetics, IRCCS S. De Bellis, Castellana Grotte (BA).
02/2023-06/2023
Volunteer at Laboratory of Medical Genetics, IRCCS S. De Bellis, Castellana Grotte (BA).
11/2021-11/2022
Undergraduate training at Laboratory of Biochemistry and protein engineering, Department of Life Sciences, University of Modena e Reggio Emilia (Modena). Project: “Analisi degli effetti della palmitoiletanolamide (PEA) veicolata da nanoparticelle su vitalità e differenziamento di cellule muscolari murine”.
03/2020-06/2020
Undergraduate training at Laboratory of Microbiology, Department of Biological and Environmental Sciences and Technologies, University of Salento (Lecce). Project: “Identificazione e analisi del profilo trascrizionale degli RNA non codificanti in Streptomyces Ambofaciens, produttore dell’antibiotico spiramicina”.
CARRER:
16/12/2022
Master degree in Medical Biotechnology and Regenerative Medicine (LM-9), Department of Life Sciences, University of Modena e Reggio Emilia - Modena, Italy
21/07/2020
Bachelor degree in Biotechnology, Department of Biological and Environmental Sciences and Technologies, University of Salento - Lecce, Italy
SKILLS
Cellular biology:
2D cell culture (cancer cells, cancer stem cells), 3D culture (patient-derived tumor organoids and stem cells), bacteria culture, Transfection assay, Optical Microscopy. Cell viability assay, Citotoxicity assay, tumorigenesis assay, cell synchronization techniques.
Histology techniques:
Immunofluorescence techniques.
Molecular Biology techniques:
PCR, quantitative real time PCR, RT-PCR, cloning, plasmidic DNA extraction, RNA and DNA extraction, Reverse transcription, Chromatin Immunoprecipitation (ChIP).
Biochemical analyses:
Western Blot analysis, Recombinant protein expression and purification, Protein Activity Assay, Co-immunoprecipitation.
Computer skills: Windows and Mac OS platform. Office. Photoshop. ImageLab. ImageJ. ZEISS ZEN software. QuantaSoft software.