ELENA GUGOLE

Dottoressa di ricerca

ciclo: XXXV


supervisore: Giuliano Sciara
relatore: Vallone Beatrice
co-supervisore: Cutruzzolà Francesca

Titolo della tesi: Novel insights into fungal degradation of lignin: role of a flavoenzyme in assisting laccase-mediated oxidations.

Lignin is the second most abundant biopolymer on earth. It could be a source of aromatic compounds of biotechnological and pharmaceutical interest; however, its heterogeneous and extremely complex chemical structure makes its valorization scarce. Filamentous fungi are efficient degraders of lignin owing to the secretion of a wide arsenal of redox proteins, such as laccase enzymes, which perform an enzymatic combustion. Attempts to reproduce laccase oxidation on lignin-derived compounds in vitro, yielded in repolymerization, resulting in recalcitrant lignin. It has been suggested that oligosaccharide dehydrogense from Pycnoporus cinnabarinus (PcODH), together with other fungal flavoenzymes classified in the “Auxiliary activities” class from the CAZy database, are involved in avoiding repolymerization in vivo. In this thesis, the structural and functional characterization of PcODH, provides new insights into its role as an auxiliary enzyme, supporting laccase during the lignin degradation process.

Produzione scientifica

  • 11573/1687998 - 2023 - Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR (01a Articolo in rivista)
    FREDA, IDA; EXERTIER, CECILE; BARILE, ANNA; VIVOLI VEGA, MIRELLA; GUGOLE, ELENA; SCIPIONI, ANITA; SAVINO, CARMELINDA; DI SALVO, MARTINO LUIGI; CONTESTABILE, ROBERTO; TRAMONTI, ANGELA; MONTEMIGLIO, LINDA CELESTE
  • 11573/1665426 - 2023 - Effect of salts on the conformational dynamics of the Cytochrome P450 OleP (01a Articolo in rivista)
    DE SCISCIO, MARIA LAURA; NARDI, ALESSANDRO NICOLA; PARISI, GIACOMO; BULFARO, GIOVANNI; COSTANZO, ANTONELLA; GUGOLE, ELENA; FREDA, IDA; SAVINO, CARMELINDA; VALLONE, BEATRICE; MONTEMIGLIO, LINDA CELESTE; D'ABRAMO, MARCO
  • 11573/1441957 - 2020 - Dissecting the cytochrome P450 OleP substrate specificity: evidence for a preferential substrate (01a Articolo in rivista)
    PARISI, GIACOMO; FREDA, IDA; EXERTIER, CECILE; CECCHETTI, CRISTINA; GUGOLE, ELENA; CERUTTI, GABRIELE; MACONE, ALBERTO; VALLONE, BEATRICE; SAVINO, CARMELINDA; MONTEMIGLIO, LINDA CELESTE
  • 11573/1689297 - 2023 - Novel insights into fungal degradation of lignin: role of a flavoenzyme in assisting laccase-mediated oxidations (07a Tesi di Dottorato)
    GUGOLE, ELENA
  • 11573/1564453 - 2021 - Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose (01a Articolo in rivista)
    CERUTTI, GABRIELE; GUGOLE, ELENA; MONTEMIGLIO, LINDA CELESTE; EXERTIER, CECILE; FREDA, IDA; VALLONE, BEATRICE; SAVINO, CARMELINDA; SCIARA, GIULIANO
  • 11573/1607625 - 2022 - Point mutations at a key site alter the cytochrome P450 oleP structural dynamics (01a Articolo in rivista)
    MONTEMIGLIO, LINDA CELESTE; GUGOLE, ELENA; FREDA, IDA; EXERTIER, CECILE; CHEN, CHENG GIUSEPPE; NARDI, ALESSANDRO NICOLA; CERUTTI, GABRIELE; PARISI, GIACOMO; D'ABRAMO, MARCO; SAVINO, CARMELINDA; VALLONE, BEATRICE
  • 11573/1617165 - 2022 - Neuroglobin, clues to function and mechanism (01g Articolo di rassegna (Review))
    EXERTIER, CECILE; MONTEMIGLIO, LINDA CELESTE; FREDA, IDA; GUGOLE, ELENA; SAVINO, CARMELINDA; VALLONE, BEATRICE
  • 11573/1652940 - 2022 - ALS2-related motor neuron diseases: from symptoms to molecules (01g Articolo di rassegna (Review))
    GUGOLE, ELENA; VALLONE, BEATRICE
  • 11573/1652905 - 2022 - Probing the role of murine neuroglobin CDloop-D-Helix unit in CO ligand binding and structural dynamics (01a Articolo in rivista)
    FREDA, IDA; GUGOLE, ELENA; CERUTTI, GABRIELE; PARISI, GIACOMO; MONTEMIGLIO, LINDA CELESTE; BRUNO, STEFANO; SAVINO, CARMELINDA; ZAMPARELLI, CARLOTTA; VALLONE, BEATRICE

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma