ELENA GUGOLE

Dottoressa di ricerca

ciclo: XXXV


supervisore: Giuliano Sciara
relatore: Vallone Beatrice
co-supervisore: Cutruzzolà Francesca

Titolo della tesi: Novel insights into fungal degradation of lignin: role of a flavoenzyme in assisting laccase-mediated oxidations.

Lignin is the second most abundant biopolymer on earth. It could be a source of aromatic compounds of biotechnological and pharmaceutical interest; however, its heterogeneous and extremely complex chemical structure makes its valorization scarce. Filamentous fungi are efficient degraders of lignin owing to the secretion of a wide arsenal of redox proteins, such as laccase enzymes, which perform an enzymatic combustion. Attempts to reproduce laccase oxidation on lignin-derived compounds in vitro, yielded in repolymerization, resulting in recalcitrant lignin. It has been suggested that oligosaccharide dehydrogense from Pycnoporus cinnabarinus (PcODH), together with other fungal flavoenzymes classified in the “Auxiliary activities” class from the CAZy database, are involved in avoiding repolymerization in vivo. In this thesis, the structural and functional characterization of PcODH, provides new insights into its role as an auxiliary enzyme, supporting laccase during the lignin degradation process.

Produzione scientifica

11573/1715634 - 2024 - Binding of steroid substrates reveals the key to the productive transition of the cytochrome P450 OleP
Costanzo, Antonella; Fata, Francesca; Freda, Ida; De Sciscio, Maria Laura; Gugole, Elena; Bulfaro, Giovanni; Di Renzo, Matteo; Barbizzi, Luca; Exertier, Cécile; Parisi, Giacomo; D’Abramo, Marco; Vallone, Beatrice; Savino, Carmelinda; Montemiglio, Linda Celeste - 01a Articolo in rivista
rivista: STRUCTURE (London: Current Biology.) pp. - - issn: 0969-2126 - wos: (0) - scopus: (0)

11573/1665426 - 2023 - Effect of salts on the conformational dynamics of the Cytochrome P450 OleP
De Sciscio, Maria Laura; Nardi, Alessandro Nicola; Parisi, Giacomo; Bulfaro, Giovanni; Costanzo, Antonella; Gugole, Elena; Exertier, Cécile; Freda, Ida; Savino, Carmelinda; Vallone, Beatrice; Montemiglio, Linda Celeste; D’Abramo, Marco - 01a Articolo in rivista
rivista: MOLECULES (Basel: MDPI Berlin: Springer, 1996-) pp. - - issn: 1420-3049 - wos: WOS:000927697700001 (0) - scopus: 2-s2.0-85146781706 (0)

11573/1687998 - 2023 - Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR
Freda, Ida; Exertier, Cecile; Barile, Anna; Chaves-Sanjuan, Antonio; Vivoli Vega, Mirella; Isupov, Michail N.; Harmer, Nicholas J.; Gugole, Elena; Swuec, Paolo; Bolognesi, Martino; Scipioni, Anita; Savino, Carmelinda; Di Salvo, Martino Luigi; Contestabile, Roberto; Vallone1, Beatrice; Tramonti, Angela; Montemiglio, Linda Celeste - 01a Articolo in rivista
rivista: NUCLEIC ACIDS RESEARCH (Oxford University Press:Journals Department, Great Clarendon Street, Oxford OX2 6DP United Kingdom:011 44 1865 556767, EMAIL: jnlorders@oup.co.uk, INTERNET: http://www.oup.co.uk, Fax: 011 44 1865 267485 Editore precedente: Information Retrieval ltd., London) pp. 8237-8254 - issn: 0305-1048 - wos: WOS:001029470200001 (1) - scopus: 2-s2.0-85170282541 (1)

11573/1708705 - 2023 - Structural insights into the DNA recognition mechanism by the bacterial transcription factor PdxR
Freda, Ida; Exertier, Cécile; Barile, Anna; Chaves-Sanjuan, Antonio; Vivoli Vega, Mirella; Isupov, Michail N; Harmer, Nicholas J; Gugole, Elena; Swuec, Paolo; Bolognesi, Martino; Scipioni, Anita; Savino, Carmelinda; Di Salvo, Martino Luigi; Contestabile, Roberto; Vallone, Beatrice; Tramonti, Angela; Montemiglio, Linda Celeste - 01a Articolo in rivista
rivista: NUCLEIC ACIDS RESEARCH (Oxford University Press:Journals Department, Great Clarendon Street, Oxford OX2 6DP United Kingdom:011 44 1865 556767, EMAIL: jnlorders@oup.co.uk, INTERNET: http://www.oup.co.uk, Fax: 011 44 1865 267485 Editore precedente: Information Retrieval ltd., London) pp. 8237-8254 - issn: 0305-1048 - wos: WOS:001029470200001 (1) - scopus: 2-s2.0-85170282541 (1)

11573/1617165 - 2022 - Neuroglobin, clues to function and mechanism
Exertier, C.; Montemiglio, L. C.; Freda, I.; Gugole, E.; Parisi, G.; Savino, C.; Vallone, B. - 01g Articolo di rassegna (Review)
rivista: MOLECULAR ASPECTS OF MEDICINE (Oxford: Elsevier Science Limited Oxford; New York: Pergamon Press 1976-) pp. 101055- - issn: 0098-2997 - wos: WOS:000771745400008 (12) - scopus: 2-s2.0-85120642758 (15)

11573/1652905 - 2022 - Probing the role of murine neuroglobin CDloop-D-Helix unit in CO ligand binding and structural dynamics
Exertier, Cécile; Sebastiani, Federico; Freda, Ida; Gugole, Elena; Cerutti, Gabriele; Parisi, Giacomo; Montemiglio, Linda Celeste; Becucci, Maurizio; Viappiani, Cristiano; Bruno, Stefano; Savino, Carmelinda; Zamparelli, Carlotta; Anselmi, Massimiliano; Abbruzzetti, Stefania; Smulevich, Giulietta; Vallone, Beatrice - 01a Articolo in rivista
rivista: ACS CHEMICAL BIOLOGY (AMERICAN CHEMICAL SOCiety, 1155 16TH ST, NW, WASHINGTON, USA, DC, 20036) pp. 2099-2108 - issn: 1554-8929 - wos: WOS:000841148100001 (2) - scopus: 2-s2.0-85136910550 (3)

11573/1652940 - 2022 - ALS2-related motor neuron diseases: from symptoms to molecules
Miceli, Marcello; Exertier, Cécile; Cavaglià, Marco; Gugole, Elena; Boccardo, Marta; Casaluci, Rossana Rita; Ceccarelli, Noemi; De Maio, Alessandra; Vallone, Beatrice; Deriu, Marco A - 01g Articolo di rassegna (Review)
rivista: BIOLOGY (Basel : MDPI) pp. - - issn: 2079-7737 - wos: WOS:000913527900001 (9) - scopus: 2-s2.0-85122736852 (9)

11573/1607625 - 2022 - Point mutations at a key site alter the cytochrome P450 oleP structural dynamics
Montemiglio, L. C.; Gugole, E.; Freda, I.; Exertier, C.; D'auria, L.; Chen, C. G.; Nardi, A. N.; Cerutti, G.; Parisi, G.; D'abramo, M.; Savino, C.; Vallone, B. - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. 55- - issn: 2218-273X - wos: WOS:000747839400001 (4) - scopus: 2-s2.0-85122022030 (5)

11573/1564453 - 2021 - Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose
Cerutti, Gabriele; Gugole, Elena; Montemiglio, Linda Celeste; Turbé-Doan, Annick; Chena, Dehbia; Navarro, David; Lomascolo, Anne; Piumi, François; Exertier, Cécile; Freda, Ida; Vallone, Beatrice; Record, Eric; Savino, Carmelinda; Sciara, Giuliano - 01a Articolo in rivista
rivista: BIOTECHNOLOGY FOR BIOFUELS (London : BioMed Central.) pp. 161- - issn: 1754-6834 - wos: WOS:000678992600001 (7) - scopus: 2-s2.0-85111172719 (7)

11573/1441957 - 2020 - Dissecting the cytochrome P450 OleP substrate specificity: evidence for a preferential substrate
Parisi, Giacomo; Freda, Ida; Exertier, Cecile; Cecchetti, Cristina; Gugole, Elena; Cerutti, Gabriele; D'auria, Lucia; Macone, Alberto; Vallone, Beatrice; Savino, Carmelinda; Montemiglio, Linda Celeste - 01a Articolo in rivista
rivista: BIOMOLECULES (Basel: MDPI) pp. - - issn: 2218-273X - wos: WOS:000584021500001 (6) - scopus: 2-s2.0-85092397644 (6)

© Università degli Studi di Roma "La Sapienza" - Piazzale Aldo Moro 5, 00185 Roma