DONALD IELPO

Dottore di ricerca

ciclo: XXXIV



Titolo della tesi: The role of miR-34a in stress-induced neurotransmission within Dorsal Raphe Nuclei

Mounting clinical and preclinical evidence support the involvement of non-coding RNAs such as microRNAs (miRs) in the adaptive (and maladaptive) brain responses to stressful stimuli. Among miRs, the miR-34 family has been involved in the neurobiological processes that underlie the regulation of stress response. Here we elucidate the mechanism through which miR-34 family modulates stress-induced neurotransmission and stress-coping behavior. First, we evaluated the expression of miR-34 family in stress-related brain areas and their role in behavioral and neurochemical stress-response. Then, using histological, molecular, and genetic approaches we examined miR-34a cellular localization in the Dorsal Raphe Nuclei (DRN), and we investigated its specific functions on neurotransmission. Finally, we evaluated a potential involvement of miR-34 family in physiological adaptations to chronic stress exposure. We found that, within brain, miR-34 (especially the isoform a) is highly expressed in DRN. Here it plays a modulatory role on Serotoninergic release to projection areas, through a fine inhibitory tuning of GABA release, thus determining the adopted coping response to acute and chronic stressors. Finally, we identified through next-gen RISC-RNA sequencing several miR-34s’ target, that are possibly involved in physiological adaptations to chronic stress. This study points out that miR-34a could be a selective GABAergic regulator of the DRN activity. This factor seems to exert an inhibitory control on GABA neurons within the DRN, that in turn regulates the serotonergic activity and determines the adopted coping response to stressful stimuli.

Produzione scientifica

11573/1681565 - 2023 - Natural killer cells and innate lymphoid cells 1 tune anxiety-like behavior and memory in mice via interferon-γ and acetylcholine
Garofalo, Stefano; Cocozza, Germana; Mormino, Alessandro; Bernardini, Giovanni; Russo, Eleonora; Ielpo, Donald; Andolina, Diego; Ventura, Rossella; Martinello, Katiuscia; Renzi, Massimiliano; Fucile, Sergio; Laffranchi, Mattia; Mortari, Eva Piano; Carsetti, Rita; Sciumè, Giuseppe; Sozzani, Silvano; Santoni, Angela; Tremblay, Marie-Eve; Ransohoff, Richard M; Limatola, Cristina - 01a Articolo in rivista
rivista: NATURE COMMUNICATIONS (London: Nature Publishing Group) pp. 3103- - issn: 2041-1723 - wos: WOS:001029731000028 (6) - scopus: 2-s2.0-85160482142 (6)

11573/1688726 - 2023 - GABAergic miR-34a regulates Dorsal Raphè inhibitory transmission in response to aversive, but not rewarding, stimuli
Ielpo, Donald; Guzzo, Serafina M; Porcheddu, Giovanni F; Viscomi, Maria Teresa; Catale, Clarissa; Reverte, Ingrid; Cabib, Simona; Cifani, Carlo; Antonucci, Gabriella; Ventura, Rossella; Lo Iacono, Luisa; Marchetti, Cristina; Andolina, Diego - 01a Articolo in rivista
rivista: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (Editore National Academy of Sciences a Washington, DC) pp. - - issn: 1091-6490 - wos: (0) - scopus: 2-s2.0-85166392795 (0)

11573/1667799 - 2022 - Decline of cardiomyocyte contractile performance and bioenergetic function in socially stressed male rats
Barbetti, Margherita; Vilella, Rocchina; Dallabona, Cristina; Carla Gerra, Maria; Bocchi, Leonardo; Ielpo, Donald; Andolina, Diego; Sgoifo, Andrea; A, ; Savi, Monia; Luca Carnevali, And - 01a Articolo in rivista
rivista: HELIYON (United Kingdom: Elsevier Limited) pp. - - issn: 2405-8440 - wos: WOS:000904324400002 (2) - scopus: 2-s2.0-85141446612 (3)

11573/1659983 - 2022 - The long-lasting effects of early life adversities are sex dependent: The signature of miR-34a
D'addario, Sebastian Luca; Municchi, Diana; Mancini, Camilla; Ielpo, Donald; Babicola, Lucy; Di Segni, Matteo; Lo Iacono, Luisa; Ferlazzo, Fabio; Cifani, Carlo; Andolina, Diego; Ventura, Rossella - 01a Articolo in rivista
rivista: JOURNAL OF AFFECTIVE DISORDERS (Elsevier BV:PO Box 211, 1000 AE Amsterdam Netherlands:011 31 20 4853757, 011 31 20 4853642, 011 31 20 4853641, EMAIL: nlinfo-f@elsevier.nl, INTERNET: http://www.elsevier.nl, Fax: 011 31 20 4853598) pp. - - issn: 0165-0327 - wos: WOS:000896745200005 (1) - scopus: 2-s2.0-85142413923 (1)

11573/1700905 - 2022 - MicroRNA-34a as possible mediator for acquisition of Avoidance after Chronic Social Defeat
Papagni, Virginia; Ielpo, D; Mattei, F; Guzzo, Sm; Porcheddu, G; Lo Iacono, L; Viscomi, Mt; Carnevali, L; Marchetti, C; Cifani, C; Ventura, R; Andolina, D - 04f Poster
congresso: European Behavioral Pharmacology Society (Roma)
libro: European Behavioral Pharmacology Society - ()

11573/1571228 - 2021 - Elevated miR-34a expression and altered transcriptional profile are associated with adverse electromechanical remodeling in the heart of male rats exposed to social stress
Andolina, Diego; Monia, Savi; Ielpo, Donald; Barbetti, Margherita; Bocchi, Leonardo; Donatela, Stilli; Ventura, Rossella; Lo Iacono, Luisa; Andrea, Sgoifo; Luca, Carnevali - 01a Articolo in rivista
rivista: STRESS (PRIMO EDITORE: Luxembourg: Harwood Academic Publishers. ATTUALE Taylor & Francis Limited:Rankine Road, Basingstoke RG24 8PR United Kingdom:011 44 1256 813035, EMAIL: madeline.sims@tandf.co.uk, info@tandf.co.uk, INTERNET: http://www.tandf.co.uk, Fax: 011 44 1256 330245) pp. 621-634 - issn: 1025-3890 - wos: WOS:000670093200001 (6) - scopus: 2-s2.0-85110053468 (5)

11573/1487835 - 2021 - Long term effects of early life stress on HPA circuit in rodent models
Babicola, L.; Ventura, R.; D'addario, S. L.; Ielpo, D.; Andolina, D.; Di Segni, M. - 01a Articolo in rivista
rivista: MOLECULAR AND CELLULAR ENDOCRINOLOGY (Elsevier Science Ireland Limited:PO Box 85, Limerick Ireland:011 353 61 709600, 011 353 61 61944, EMAIL: usinfo-f@elsevier.com, INTERNET: http://www.elsevier.com, Fax: 011 353 61 709114) pp. - - issn: 0303-7207 - wos: WOS:000605607800011 (8) - scopus: 2-s2.0-85098095336 (10)

11573/1542738 - 2021 - Resilience to anhedonia-passive coping induced by early life experience is linked to a long-lasting reduction of Ih current in VTA dopaminergic neurons: altered Ih current in the VTA of adult resilient-to-depression mice
D'addario, S. L.; Di Segni, M.; Ledonne, A.; Piscitelli, R.; Babicola, L.; Martini, A.; Spoleti, E.; Mancini, C.; Ielpo, D.; D'amato, F. R.; Andolina, D.; Ragozzino, D.; Mercuri, N. B.; Cifani, C.; Renzi, M.; Guatteo, E.; Ventura, R. - 01a Articolo in rivista
rivista: NEUROBIOLOGY OF STRESS (New York : Elsevier Inc.) pp. - - issn: 2352-2895 - wos: WOS:000651603900003 (10) - scopus: 2-s2.0-85104148559 (11)

11573/1542950 - 2021 - MicroRNA-34a regulates 5-HT2C expression in dorsal raphe and contributes to the anti-depressant-like effect of fluoxetine
Lo Iacono, L.; Ielpo, D.; Parisi, C.; Napoli, G.; Accoto, A.; Di Segni, M.; Babicola, L.; D'addario, S. L.; Guzzo, S. M.; Pascucci, T.; Ventura, R.; Andolina, D. - 01a Articolo in rivista
rivista: NEUROPHARMACOLOGY (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. - - issn: 0028-3908 - wos: WOS:000663459200006 (7) - scopus: 2-s2.0-85104079200 (10)

11573/1574955 - 2021 - Early life adversity affecting the attachment bond alters ventral tegmental area transcriptomic patterning and behavior almost exclusively in female mice
Lo Iacono, Luisa; Mancini, Camilla; Babicola, Lucy; Pietrosanto, Marco; Di Segni, Matteo; D'addario, Sebastian Luca; Municchi, Diana; Ielpo, Donald; Pascucci, Tiziana; Cabib, Simona; Ferlazzo, Fabio; D'amato, Francesca R.; Andolina, Diego; Helmer-Citterich, Manuela; Cifanic, Carlo; Ventura, Rossella - 01a Articolo in rivista
rivista: NEUROBIOLOGY OF STRESS (New York : Elsevier Inc.) pp. - - issn: 2352-2895 - wos: WOS:000709723500003 (5) - scopus: 2-s2.0-85119379843 (5)

11573/1385630 - 2020 - Correction to: MicroRNA-34 contributes to the stress-related behavior and affects 5-HT prefrontal/GABA amygdalar system through regulation of corticotropin-releasing factor receptor 1
Andolina, D.; Di Segni, M.; Accoto, A.; Lo Iacono, L.; Borreca, A.; Ielpo, D.; Berretta, N.; Perlas, E.; Puglisi-Allegra, S.; Ventura, R. - 01b Commento, Erratum, Replica e simili
rivista: MOLECULAR NEUROBIOLOGY (Humana Press Incorporated:999 Riverview Drive, Suite 208:Totowa, NJ 07512:(973)256-1699, EMAIL: lglasner@humanapr.com, INTERNET: http://www.humanapress.com, Fax: (973)256-8341) pp. 586-586 - issn: 0893-7648 - wos: WOS:000520176200049 (1) - scopus: 2-s2.0-85076425355 (0)

11573/1385624 - 2020 - RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress
Babicola, L; Pietrosanto, M; Ielpo, D; D'addario, Sl; Cabib, S; Ventura, R; Ferlazzo, F; Helmer-Citterich, M; Andolina, D; Lo Iacono, L. - 01a Articolo in rivista
rivista: BRAIN RESEARCH (Amsterdam; Lausanne; New York; Oxford; Shannon; Tokyo: Elsevier) pp. - - issn: 0006-8993 - wos: WOS:000524457100010 (4) - scopus: 2-s2.0-85081672685 (4)

11573/1385627 - 2020 - Xlr4 as a new candidate gene underlying vulnerability to cocaine effects
Di Segni, M.; D'addario, S. L.; Babicola, L.; Ielpo, D.; Lo Iacono, L.; Andolina, D.; Accoto, A.; Luchetti, A.; Mancini, C.; Parisi, C.; D'onofrio, M.; Arisi, I.; Brandi, R.; Pascucci, T.; Cifani, C.; D'amato, F. R.; Ventura, R. - 01a Articolo in rivista
rivista: NEUROPHARMACOLOGY (Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010) pp. - - issn: 0028-3908 - wos: WOS:000528256100036 (3) - scopus: 2-s2.0-85081004285 (3)

11573/1291946 - 2019 - Sex-dependent effects of early unstable post-natal environment on response to positive and negative stimuli in adult mice
Di Segni, M; Andolina, D; D'addario, Sl; Babicola, L; Ielpo, D; Luchetti, A; Pascucci, T; Lo Iacono, L; D'amato, Fr; Ventura, R. - 01a Articolo in rivista
rivista: NEUROSCIENCE (Oxford: Elsevier Ltd. Oxford; New York: Pergamon Press) pp. 1-10 - issn: 0306-4522 - wos: WOS:000476866600001 (12) - scopus: 2-s2.0-85067853998 (14)

11573/1307879 - 2019 - MicroRNA-34a regulates the depression-like behavior in mice by modulating the expression of target genes in the Dorsal Raphè
Lo Iacono, Luisa; Ielpo, Donald; Accoto, Alessandra; Di Segni, Matteo; Babicola, Lucy; D'addario, Sebastian Luca; Ferlazzo, Fabio; Pascucci, Tiziana; Ventura, Rossella; Andolina, Diego - 01a Articolo in rivista
rivista: MOLECULAR NEUROBIOLOGY (Humana Press Incorporated:999 Riverview Drive, Suite 208:Totowa, NJ 07512:(973)256-1699, EMAIL: lglasner@humanapr.com, INTERNET: http://www.humanapress.com, Fax: (973)256-8341) pp. - - issn: 0893-7648 - wos: WOS:000515028000017 (15) - scopus: 2-s2.0-85071772292 (18)

11573/1073617 - 2018 - MicroRNA-34 Contributes to the Stress-related Behavior and Affects 5-HT Prefrontal/GABA Amygdalar System through Regulation of Corticotropin-releasing Factor Receptor 1
Andolina, D; Di Segni, M; Accoto, A; Lo Iacono, L; Borreca, A; Ielpo, Donald; Berretta, N; Perlas, E; Puglisi-Allegra, S; Ventura, R - 01a Articolo in rivista
rivista: MOLECULAR NEUROBIOLOGY (Humana Press Incorporated:999 Riverview Drive, Suite 208:Totowa, NJ 07512:(973)256-1699, EMAIL: lglasner@humanapr.com, INTERNET: http://www.humanapress.com, Fax: (973)256-8341) pp. - - issn: 0893-7648 - wos: WOS:000440453100023 (19) - scopus: 2-s2.0-85041507704 (20)

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