VALERIO DE FRANCHIS

Dottore di ricerca

ciclo: XXXVI


supervisore: Prof.ssa Claudia Giampietri

Titolo della tesi: Cholangiocarcinoma malignant traits are promoted by Schwann cell-derived Transforming Growth Factor-beta in Perineural invasion

The term cholangiocarcinoma (CCA) defines a class of epithelial malignancies originating from bile ducts. Although it has been demonstrated that CCA patients with perineural invasion (PNI) have a worse prognosis, the biological features of this phenomenon are yet unclear. Our data show that in human intrahepatic CCA specimens with documented PNI, nerve-infiltrating CCA cells display an expression level of the epithelial marker cytokeratin 7 lower respect to the rest of the tumor mass. In an in vitro 3D model, CCA cells move towards a peripheral nerve explant getting contact with Schwann cells (SCs) emerging from the nerve. Here we show that SCs produce soluble factors that favor the migration, invasion, survival and proliferation of CCA cells in vitro. This effect is accompanied by a cadherin switch suggestive of an epithelial-mesenchymal transition. The influence of SCs in promoting the ability of CCA cells to migrate and to invade the extracellular matrix is hampered by a specific TGFβ receptor 1 (Tβ1R) antagonist. Differential proteomic data indicate that the exposure of CCA cells to SCs secreted factors induces the upregulation of key oncogenes and the concomitant downregulation of some tumor suppressors. Taken together these data concur in identifying SCs as possible promoters of a more aggressive CCA phenotype ascribing to TGFβ-signaling a central role in regulating this process.

Produzione scientifica

11573/1703368 - 2024 - Cholangiocarcinoma Malignant Traits Are Promoted by Schwann Cells through TGFβ Signaling in a Model of Perineural Invasion
De Franchis, Valerio; Petrungaro, Simonetta; Pizzichini, Elisa; Camerini, Serena; Casella, Marialuisa; Somma, Francesca; Mandolini, Enrico; Carpino, Guido; Overi, Diletta; Cardinale, Vincenzo; Facchiano, Antonio; Filippini, Antonio; Gaudio, Eugenio; Fabrizi, Cinzia; Giampietri, Claudia - 01a Articolo in rivista
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. - - issn: 2073-4409 - wos: (0) - scopus: (0)

11573/1690588 - 2023 - Role of TGF-ß as a potential molecule for Schwann cell-induced Cholangiocarcinoma progression
De Franchis, Valerio; Petrungaro, Simonetta; Somma, Francesca; Mandolini, Enrico; Luisa Casella, Maria; Camerini, Serena; Pompili, Elena; Facchiano, Antonio; Filippini, Antonio; Fabrizi, Cinzia; Gaudio, Eugenio; Giampietri, Claudia - 04d Abstract in atti di convegno
congresso: 76° Congresso della Società Italiana di Anatomia e Istologia (Modena)
libro: Italian Journal of Anatomy and Embriology - ()

11573/1664172 - 2023 - Autophagy Activation Associates with Suppression of Prion protein and Improved Mitochondrial Status in Glioblastoma Cells
Lenzi, Paola; Busceti, Carla; Lazzeri, Gloria; Ferese, Rosangela; Biagioni, Francesca; Salvetti, Alessandra; Pompili, Elena; De Franchis, Valerio; Puglisi-Allegra, Stefano; Frati, Alessandro; Ferrucci, Michela; Fornai, Francesco - 01a Articolo in rivista
rivista: CELLS (Basel: mdpi-Molecular Diversity Preservation International) pp. 221- - issn: 2073-4409 - wos: WOS:000917400500001 (4) - scopus: 2-s2.0-85146808622 (4)

11573/1690266 - 2023 - Autophagy impairment in human bile duct carcinoma cells
Petrungaro, Simonetta; De Franchis, Valerio; Filippini, Antonio; Facchiano, Antonio; Gaudio, Eugenio; Giampietri, Claudia - 01a Articolo in rivista
rivista: FRONTIERS IN PHYSIOLOGY (Lausanne: Frontiers Research Foundation, 2010-) pp. 1-9 - issn: 1664-042X - wos: WOS:001085700100001 (0) - scopus: 2-s2.0-85174155803 (1)

11573/1655580 - 2022 - Cancer-nerve crosstalk in human cholangiocarcinoma
De Franchis, Valerio; Petrungaro, Simonetta; Mandolini, Enrico; Somma, Francesca; Pompili, Elena; Filippini, Antonio; Gaudio, Eugenio; Fabrizi, Cinzia; Giampietri, Claudia - 04d Abstract in atti di convegno
rivista: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY (Firenze : Firenze university press) pp. 47-47 - issn: 2038-5129 - wos: (0) - scopus: (0)
congresso: 75° Congresso della Società Italiana di Anatomia e Istologia (Padova)
libro: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY - ()

11573/1655867 - 2022 - The thrombin/PAR1 axis as regulator of Schwann cell functions in health and disease
Fabrizi, Cinzia; Mantua, Benedetta; De Santis, Elena; Giampietri, Claudia; Somma, Francesca; De Franchis, Valerio; Pompili, Elena - 04f Poster
rivista: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY (Firenze : Firenze university press) pp. 227-227 - issn: 2038-5129 - wos: (0) - scopus: (0)
congresso: 75° CONGRESSO della Società Italiana di Anatomia e Istologia (Padova)
libro: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY - ()

11573/1656329 - 2022 - Cross-talk between autophagy and apoptosis in an experimental in vitro model of cholangiocarcinoma
Giampietri, Claudia; Petrungaro, Simonetta; De Franchis, Valerio; Filippini, Antonio; Gaudio, Eugenio - 04d Abstract in atti di convegno
rivista: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY (Firenze : Firenze university press) pp. 128-128 - issn: 2038-5129 - wos: (0) - scopus: (0)
congresso: 75° Congresso della Società Italiana di Anatomia e Istologia (Padova)
libro: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY - ()

11573/1677627 - 2021 - Cancer-nerve crosstalk in human cholangiocarcinoma
De Franchis, Valerio; Petrungaro, Simonetta; Pompili, Elena; Carpino, Guido; Filippini, Antonio; Gaudio, Eugenio; Fabrizi, Cinzia; Giampietri, Claudia - 01h Abstract in rivista
rivista: ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY (Mozzon Giuntina SPA:Via Mannelli 29R, I 50136 Florence Italy:011 39 55 2476781, EMAIL: sedicesimo@fol.it, Fax: 011 39 55 2349067 Firenze: Editrice Il Sedicesimo.) pp. 169-169 - issn: 1122-6714 - wos: (0) - scopus: (0)

11573/1481110 - 2021 - Anti-tumor effect of oleic acid in hepatocellular carcinoma cell lines via autophagy reduction
Giulitti, Federico; Petrungaro, Simonetta; Mandatori, Sara; Tomaipitinca, Luana; De Franchis, Valerio; D'amore, Antonella; Filippini, Antonio; Gaudio, Eugenio; Ziparo, Elio; Giampietri, Claudia - 01a Articolo in rivista
rivista: FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (Lausanne : Frontiers Media S.A., 2013-) pp. 1-16 - issn: 2296-634X - wos: WOS:000619466300001 (41) - scopus: 2-s2.0-85101221482 (53)

11573/1585681 - 2021 - Protease activated receptor 1 and its ligands as main regulators of the regeneration of peripheral nerves
Pompili, E.; De Franchis, V.; Giampietri, C.; Leone, S.; De Santis, E.; Fornai, F.; Fumagalli, L.; Fabrizi, C. - 01g Articolo di rassegna (Review)
rivista: BIOMOLECULES (Basel: MDPI) pp. 1-15 - issn: 2218-273X - wos: WOS:000727344000001 (5) - scopus: 2-s2.0-85118695308 (5)

11573/1382858 - 2020 - Thrombin regulates the ability of Schwann cells to support neuritogenesis and to maintain the integrity of the nodes of Ranvier
Pompili, Elena; Ciraci, Viviana; Leone, Stefano; De Franchis, Valerio; Familiari, Pietro; Matassa, Roberto; Familiari, Giuseppe; Tata, Ada Maria; Fumagalli, Lorenzo; Fabrizi, Cinzia - 01a Articolo in rivista
rivista: EUROPEAN JOURNAL OF HISTOCHEMISTRY (Pavia : PagePress Publications, 2010) pp. 104-113 - issn: 2038-8306 - wos: WOS:000551388600006 (12) - scopus: 2-s2.0-85082791799 (12)

11573/1391350 - 2019 - Protease-activated receptors in Schwann cells.
Fabrizi, C; Pompili, E; Leone, S; De Franchis, V; Masucci, M; Fumagalli, L. - 04d Abstract in atti di convegno
congresso: 73° Congresso della Società Italiana di Anatomia e Istologia (Napoli)
libro: it j anat embryol - ()

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