DANIELE DEL CORPO

Dottore di ricerca

ciclo: XXXII



Titolo della tesi: Tuning Pectin Methylesterification in Plant Immunity: Post-Transcriptional Regulation of Pectin Methylesterases by Subtilases

The plant cell wall (CW) is the foremost barrier where plant-pathogen interactions occur. The CW is a complex and dynamic compartment composed of cellulose micro-fibrils embedded in a dense network of hemicelluloses and pectins. Pectins are synthesized in a highly methylesterified form in the Golgi cisternae and partially de-methylesterified in muro by plant Pectin Methylesterases (PMEs). In Arabidopsis thaliana there are 66 PME isoforms involved in important development processes and in plant immunity during pathogenesis. 45 isoforms are ProPMEs and possess, in addition to the PME domain, an N-terminal Pro-region that shows sequence similarities with the PME Inhibitors. A. thaliana induces the expression of specific PME isoforms during the attack of the necrotrophic fungus Botrytis cinerea. The amino acidic sequences alignment of these isoforms shows that they all are ProPMEs. Among them, AtProPME17 is particularly interesting since it is induced after the infection of a wide-range of plant pathogens and can be considered a general biomarker of pathogenesis. ProPME activity can be post-transcriptionally regulated by specific Subtilases (SBTs), serine-like proteases that process conserved recognition sites releasing the PME domain from the Pro-region. AtSBT3.3, AtSBT3.4 and AtSBT3.5 were found to be temporally co-expressed with AtProPME17 in A. thaliana plants during B. cinerea infection and selected for further analysis. Homozygous A. thaliana T-DNA insertional mutants have been isolated and characterized. During the infection process, the decreased expression of AtSBT3.3 and AtProPME17 in their respective mutants determines a lower increase of PME activity and an increased susceptibility to B. cinerea respect to the control plants. By exploiting heterologous expression in Pichia pastoris and Escherichia coli, it has been observed that AtProPME17 is a functional PME performing a blockwise pattern of pectin de-methylesterification and showing an optimal activity at alkaline pH. Moreover, we demonstrated that the Pro-region acts as an intramolecular inhibitor of AtProPME17 activity.

Produzione scientifica

11573/1696995 - 2023 - Pro-pectin methylesterases as zymogens for plant cell wall mediated immunity
Coculo, Daniele; Del Corpo, Daniele; Lionetti, Vincenzo - 04d Abstract in atti di convegno
congresso: Plant Cell Wall Meeting: XVI PCWM 2023 (Málaga, Spain)
libro: Abstract book XVI Plant Cell Wall Meeting 2023 - ()

11573/1685957 - 2023 - Arabidopsis subtilases promote defense-related pectin methylesterase activity and robust immune responses to botrytis infection
Coculo, Daniele; Del Corpo, Daniele; Ozáez Martínez, Miguel; Vera, Pablo; Piro, Gabriella; De Caroli, Monica; Lionetti, Vincenzo - 01a Articolo in rivista
rivista: PLANT PHYSIOLOGY AND BIOCHEMISTRY (-Paris: Elsevier 1998- -Montrouge Cedex France: Gauthier-Villars) pp. - - issn: 0981-9428 - wos: WOS:001042891700001 (3) - scopus: 2-s2.0-85165323327 (5)

11573/1695998 - 2023 - SBT3.3 and pro-PME17 are secreted through distinct protein secretion pathways in the apoplast
Coculo, Daniele; Del Corpo, Daniele; Piro, Gabriella; De Caroli, Monica; Lionetti, Vincenzo - 04d Abstract in atti di convegno
congresso: Plant Cell Wall Meeting: XVI PCWM 2023 (Málaga, Spain)
libro: Abstract book XVI Plant Cell Wall Meeting 2023 - ()

11573/1696992 - 2023 - Protein precursors for effective plant immunity: the case of pectin methylesterases
Coculo, Daniele; Greco, Marco; Del Corpo, Daniele; Lionetti, Vincenzo - 04d Abstract in atti di convegno
congresso: XII Congress of the Italian Society of Plant Biology (Bari, Italy)
libro: Abstract book XII Congress of the Italian Society of Plant Biology - ()

11573/1696997 - 2022 - Subtilases trigger pectin-related immunity against pathogens
Coculo, Daniele; Del Corpo, Daniele; Lionetti, Vincenzo - 04d Abstract in atti di convegno
congresso: IX Cell Wall Research Conference (East Lansing, Michigan, U.S.A.)
libro: Abstract book IX Cell Wall Research Conference - ()

11573/1695987 - 2021 - Molecular factors underlying Arabidopsis PME activation against Botrytis
Coculo, Daniele; Pecatelli, Gabriele; Del Corpo, Daniele; Lionetti, Vincenzo - 04f Poster
congresso: PLANT BIOLOGY EUROPE 2021 (Turin, Italy)
libro: Abstract book PBE 2021 - ()

11573/1556642 - 2021 - New insight into the biochemical features of AtPME17, a functional Arabidopsis PME affecting plant resistance to pathogens, regulated by its pro-region
Miele, Rossella; Rosari Fullone, Maria; Del Corpo, Daniele; Pontiggia, Daniela; Vittozzi, Laura; Di Matteo, Adele; Giardina, Thierry; Duquesne, Katia; Lionetti, Vincenzo; Bellincampi, Daniela - 04d Abstract in atti di convegno
congresso: Plant Biology Europe 2021 (Tourin ( webinar meeting))
libro: Book of absracts Plant Biology Europe 2021 - ()

11573/1467444 - 2020 - AtPME17 is a functional arabidopsis thaliana pectin methylesterase regulated by its PRO region that triggers PME activity in the resistance to botrytis cinerea
Del Corpo, D.; Fullone, M. R.; Miele, R.; Lafond, M.; Pontiggia, D.; Grisel, S.; Kieffer-Jaquinod, S.; Giardina, T.; Bellincampi, D.; Lionetti, V. - 01a Articolo in rivista
rivista: MOLECULAR PLANT PATHOLOGY (Blackwell Science) pp. 1620-1633 - issn: 1464-6722 - wos: WOS:000575685000001 (37) - scopus: 2-s2.0-85092202911 (35)

11573/1326675 - 2019 - In silico identification of structural signatures in Arabidopsis thaliana pectin methylesterases
Bellincampi, Daniela; Andrea, Casciano; Del Corpo, Daniele; Lionetti, Vincenzo; Pascarella, Stefano - 04d Abstract in atti di convegno
congresso: 11th congress of the italian society of plant biology (Padova)
libro: SIBV - Abstract Book - ()

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