Research: Using Crispr-Cas9 technology to investigate the role of NBS1 mutations on ciliogenesis as a model for the Nijmegen breakage syndrome.
WORK ADDRESS:
Department of Molecular Medicine, Viale Regina Elena 291, Roma
SUPERVISOR:
Prof. Marella Maroder
PREVIOUS RESEARCH EXPERIENCE:
September 2020 – October 2021 Student Internship in the Molecular Oncology laboratory (Prof. A. Gulino), Department of Molecular Medicine, University of Rome ‘La Sapienza’
Research lines:.Role of Nbs1 in cerebellar development and tumorigenesis
CAREER:
2021 – today: PhD student in Molecular Medicine at Department of Molecular Medicine, University of Rome ‘La Sapienza’.
2019 –2021 : Master’s degree in Medical Biotechnology at ‘Sapienza’ University of Rome. Graduation Cum Laude.
2016-2019 - Bachelor's Degree in Biotechnology at University of Rome Tor Vergata.
SKILLS:
Maintenance of primary and immortalized cell cultures
Generation of primary cultures from tissue
Transient transfection of nucleic acids
Infection with virus
RNA and DNA extraction and purification, PCR, RT-PCR and Q-PCR
Protein extraction, Bradford Assay, Western Blot,
Immunoprecipitation
Use of cryostat and staining of cryosections
Immunohistochemistry
Immunofluorescence
Light and confocal microscopy
Proliferative Test: BrdU/EdU