DAISY PROIETTI

Dottoressa di ricerca

ciclo: XXXIII


supervisore: Luca Madaro

Titolo della tesi: “MOLECULAR AND CELLULAR NETWORKS DRIVING NEUROGENIC MUSCLE ATROPHY”

During neurogenic muscle atrophy, the interruption of transmission of neurogenic signals to muscles, caused by loss of neuromuscular junction (NMJ) integrity, leads to muscle atrophy, an event that causes loss of muscle functionality with the obvious deleterious outcomes. NMJs degeneration is a prominent aspect of denervation, aging, and some pathological conditions, such as Amyotrophic Lateral Sclerosis (ALS). The precise molecular mechanisms and roles of muscle-resident cells, during neurogenic muscle atrophy, remain largely unknown. Evidence suggests that the effect on myofibers caused by neuron loss involves neighboring resident cells. Therefore, we analyzed the gene expression profiles at bulk and single-cell level of Itga7-expressing cells in muscle and revealed the existence of a subpopulation of muscle-resident glial cells, distinct from muscle satellite cells, that is selectively activated upon nerve injury. Upon nerve lesion, these cells expand and activate a neurotrophic program, that is turn off upon recovery of NMJ integrity. Moreover, these muscle-resident glial cells in the ALS mouse model increase during disease progression but exhibit impaired neurotrophic activity, suggesting that defective activation of glial cells could be implicated in ALS pathogenesis. Therefore, a better understanding of the cellular and molecular signaling pathways in muscle-resident cells during traumatic or pathological denervation is critical for developing better therapies against neurogenic muscle atrophy and degenerative diseases such as ALS.

Produzione scientifica

11573/1662908 - 2022 - Spatially resolved transcriptomics reveals innervation-responsive functional clusters in skeletal muscle
D'ercole, Chiara; D'angelo, Paolo; Ruggieri, Veronica; Proietti, Daisy; Virtanen, Laura; Parisi, Cristina; Riera, Carles Sanchez; Renzini, Alessandra; Macone, Alberto; Marzullo, Marta; Ciapponi, Laura; Bonvissuto, Davide; Sette, Claudio; Giordani, Lorenzo; Madaro, Luca - 01a Articolo in rivista
rivista: CELL REPORTS (Elsevier B.V. Cambridge MA: Cell Press) pp. 1-23 - issn: 2211-1247 - wos: WOS:000912450400001 (13) - scopus: 2-s2.0-85144347410 (13)

11573/1551537 - 2021 - Activation of skeletal muscle-resident glial cells upon nerve injury
Proietti, D.; Giordani, L.; De Bardi, M.; D'ercole, C.; Lozanoska-Ochser, B.; Amadio, S.; Volonte, C.; Marinelli, S.; Muchir, A.; Bouche, M.; Borsellino, G.; Sacco, A.; Puri, P. L.; Madaro, L. - 01a Articolo in rivista
rivista: JCI INSIGHT (Ann Arbor, Michigan : American Society for Clinical Investigation, [2016]-) pp. 1-16 - issn: 2379-3708 - wos: WOS:000638250400013 (15) - scopus: 2-s2.0-85103899038 (17)

11573/1412450 - 2020 - Skeletal-Muscle metabolic reprogramming in ALS-SOD1G93A mice predates disease onset and Is a promising therapeutic target
Scaricamazza, S.; Salvatori, I.; Giacovazzo, G.; Loeffler, J. P.; Rene, F.; Rosina, M.; Quessada, C.; Proietti, D.; Heil, C.; Rossi, S.; Battistini, S.; Giannini, F.; Volpi, N.; Steyn, F. J.; Ngo, S. T.; Ferraro, E.; Madaro, L.; Coccurello, R.; Valle, C.; Ferri, A. - 01a Articolo in rivista
rivista: ISCIENCE ([Cambridge MA] : Cell Press Elsevier Inc.) pp. 1-37 - issn: 2589-0042 - wos: WOS:000536743600045 (53) - scopus: 2-s2.0-85083884221 (56)

11573/1290577 - 2019 - Histaminergic transmission slows progression of amyotrophic lateral sclerosis
Apolloni, S.; Amadio, S.; Fabbrizio, P.; Morello, G.; Spampinato, A. G.; Latagliata, E. C.; Salvatori, I.; Proietti, D.; Ferri, A.; Madaro, L.; Puglisi-Allegra, S.; Cavallaro, S.; Volonte, C. - 01a Articolo in rivista
rivista: JOURNAL OF CACHEXIA, SARCOPENIA AND MUSCLE (Heidelberg ; Berlin : Springer) pp. 872-893 - issn: 2190-6009 - wos: WOS:000482975200014 (26) - scopus: 2-s2.0-85064572306 (27)

11573/1323383 - 2019 - Fibro–Adipogenic progenitors cross-talk in skeletal muscle: the social network
Biferali, B.; Proietti, Daisy; Mozzetta, C.; Madaro, L. - 01g Articolo di rassegna (Review)
rivista: FRONTIERS IN PHYSIOLOGY (Lausanne: Frontiers Research Foundation, 2010-) pp. - - issn: 1664-042X - wos: WOS:000482065900001 (144) - scopus: 2-s2.0-85072730581 (151)

11573/1212263 - 2018 - Do neurogenic and cancer-induced muscle atrophy follow common or divergent paths?
Bouchè, Marina; Lozanoska-Ochser, Biliana; Proietti, Daisy; Madaro, Luca - 01a Articolo in rivista
rivista: EUROPEAN JOURNAL OF TRANSLATIONAL MYOLOGY (- Padova : Pagepress -Padova : Unipress) pp. 393-400 - issn: 2037-7460 - wos: WOS:000455715500009 (9) - scopus: 2-s2.0-85059286275 (10)

11573/1121722 - 2018 - Role of miR-200c in myogenic differentiation impairment via p66Shc: implication in skeletal muscle regeneration of dystrophic mdx mice
D'agostino, Marco; Torcinaro, Alessio; Madaro, Luca; Marchetti, Lorenza; Sileno, Sara; Beji, Sara; Salis, Chiara; Proietti, Daisy; Imeneo, Giulia; C Capogrossi, Maurizio; De Santa, Francesca; Magenta, Alessandra - 01a Articolo in rivista
rivista: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY (London: Hindawi AUSTIN, USA, TX: LANDES BIOSCIENCE) pp. 1-10 - issn: 1942-0900 - wos: WOS:000425831200001 (19) - scopus: 2-s2.0-85048857158 (23)

11573/1290583 - 2018 - Denervation-activated STAT3–IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis
Madaro, Luca; Magda, Passafaro; David, Sala; Usue, Etxaniz; Francesca, Lugarini; Proietti, Daisy; Maria Vittoria Alfonsi, ; Chiara, Nicoletti; Sole, Gatto; Marco De Bardi, ; Ricardo, Rojas-García; Lorenzo, Giordani; Sara, Marinelli; Vittoria, Pagliarini; Claudio, Sette; Alessandra, Sacco; Pier Lorenzo, Puri. - 01a Articolo in rivista
rivista: NATURE CELL BIOLOGY (Nature America Incorporated:345 Park Avenue South, 6th Floor:New York, NY 10010:(888)331-6288, EMAIL: institutions@natureny.com, INTERNET: http://www.nature.com, Fax: (212)689-9108) pp. 917-927 - issn: 1465-7392 - wos: WOS:000440057000011 (186) - scopus: 2-s2.0-85051565989 (184)

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